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Oxygen Toxicity in the Mammalian Liver Dennis A Journal of the Arkansas Academy of Science Volume 31 Article 6 1977 Oxygen Toxicity in the Mammalian Liver Dennis A. Baeyens University of Arkansas at Little Rock M. J. Meier University of Arkansas at Little Rock Follow this and additional works at: http://scholarworks.uark.edu/jaas Part of the Biology Commons Recommended Citation Baeyens, Dennis A. and Meier, M. J. (1977) "Oxygen Toxicity in the Mammalian Liver," Journal of the Arkansas Academy of Science: Vol. 31 , Article 6. Available at: http://scholarworks.uark.edu/jaas/vol31/iss1/6 This article is available for use under the Creative Commons license: Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0). Users are able to read, download, copy, print, distribute, search, link to the full texts of these articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This Article is brought to you for free and open access by ScholarWorks@UARK. It has been accepted for inclusion in Journal of the Arkansas Academy of Science by an authorized editor of ScholarWorks@UARK. For more information, please contact [email protected], [email protected]. I Journal of the Arkansas Academy of Science, Vol. 31 [1977], Art. 6 Oxygen Toxicityinthe Mammalian Liver D.A. BAEYENS and M.J. MEIER University of Arkansas atLittleRock Department of Biology LittleRock, Ark. 72204 ABSTRACT The effect of hyperbaric oxygen tensions on the oxygen consumption of mouse liver homo- genates was investigated. Itwas found that hyperbaric oxygen rapidly inhibits the oxidative metabolism ofthe mammalian liver. Mouse liver homogenate exposed to an oxygen tension of 3837.8 mm Hg for 30 minutes demonstrated a 50.6% reduction in oxygen consumption compared to controls exposed to nitrogen at ambient pressure. The effects of reduced glutathione as a protective agent against hyperbaric oxygen tox- icity were also examined. Liver homogenates pretreated with reduced glutathione and ex- posed to hyperbaric oxygen tensions demonstrated greater activity than untreated controls. Itis concluded that: (1) Reduced glutathione protects important enzymes associated with oxidative metabolism by keeping them in a reduced and viable state, and (2) Reduced glutathione can stimulate oxygen consumption by increasing succinate formation through a reduced glutathione - succinate shunt. INTRODUCTION petri dish. The control petri dishes, one containing GSH and the other containing no, GSH, were exposed to a nitrogen environment Studies ofoxygen toxicityin vitrohave provided strong support for (Pn»=757 mm Hg) for0.5 hours. The two experimental petri dishes, the hypothesis that oxidation ofsulfhydryl groups on important tissue one containing GSH and the other without GSH, were placed in a enzymes plays an important role in the production of the symptoms table-top Bethlehem Model H-70-A Environmental Chamber. The of oxygen toxicity in vivo(Haugaard, 1968). The oxidation of sulf- chamber was filled with medical oxygen at a pressure of 3837.8 mm hydryl groups could either occur within a molecule or between differ- Hg. The total incubation time was 30 minutes, including a 15 minute ent molecules resulting in disulfide bridge formation and consequent purge with pure oxygen at a flow rate of 8 1/min. to completely enzyme inactivation. One group of sulfhydryl-containing enzymes, remove any residual gases. the dehydrogenases, has been shown to be particularly sensitive to Following the incubation period, the oxygen consumption of the le toxic effects of oxygen. An important glycolyticenzyme, lactate control and experimental tissue homogenates was measured by ehydrogenase, from the amphibian retina has been shown to be means of a YSI Model 53 Biological Oxygen Monitor. The system nhibited by exposure to normobaric oxygen tensions for 24 hours consists of a Clark-type oxygen electrode and four tissue bath cham- iaeyens and Hoffert, 1972). Inhibition ofenzymes involved inoxida- bers for the oxygen consumption measurements. The temperature of ve metabolism wouldbe reflected by a decreased oxygen consump- the oxygen electrode and the tissue chambers was regulated at on upon exposure to high oxygen tensions. Inmost in vitro studies 20±.l°C by means of a constant temperature water bath. The ealing with oxygen toxicity, the inhibitingeffects ofoxygen on cellu- electrode system was calibrated against an air-saturated solution of ar metabolism have been demonstrated only after exposure of the modified mammalian Krebs-Ringer of 3 ml vol. contained in one ssues to oxygen for a relatively long period of time or by exposing chamber of the tissue bath assembly. Each of the other three cham- lem to extremely highoxygen tensions. bers contained 3 ml ofair-saturated liver homogenate. The solutions Inthe cell, reduced glutathione (GSH) is a major sulfhydryl group were kept saturated with air by means of a magnetic stirer in each ontaining component. Reduced glutathione activates a number of chamber. To determine oxygen consumption, meter readings were nzymes, and it was proposed by Barron (1955) that the main func- taken at the start and finish of a five-minute recording period. By on of this substance is to maintain sulfhydryl groups of enzymes in substracting the concentration of oxygen remaining after five heir reduced and active state. The early rationale forusing GSH as a minutes from the initial concentration of oxygen at zero time and jrotective agent against oxygen toxicity was mainly because of this knowing the solubilitycoefficient of oxygen in mammalian Krebs- sulfhydryl-sparing effect." More recently it has been observed that Ringer ( =0.0310) (Umbreit et al., 1964) at 20°C, it was possible to GSH may stimulate oxidative metabolism by another mechanism determine the oxygen consumption of the tissue homogenate. Oxy- Sanders et al., 1969). Glutathione can be converted to succinate by a gen utilization was expressed as fi\ oi oxygen consumed per hour umber of different tissues, and this increased production of suc- per mg wet weight. Allvalues were corrected to standard tempera- inate can serve as a secondary support system for the maintenance ture and pressure dry (STPD). Astudent t-test was used to determine f high energy levels. significant differences within and between control and experimental purpose of our study was two-fold. First, does oxygen at ele- groups. an ted partial pressures exert inhibitory effect on the mammalian RESULTS er and secondly, ifsuch an inhibitory effect does exist, can it be eviated by the presence ofGSH? tThe Preliminary experiments were performed to determine ifexposing the control liverhomogenates to 100% nitrogen at ambient pressures MATERIALSANDMETHODS had any detrimental effect on oxygen consumption. There were no significant differences (P<0.1) in oxygen consumption between Male and female adult Swiss Webster mice (approximate weight homogenates incubated inroom air and those incubated in nitrogen 5-40 g) were used in all experiments. Mice were killed by cervical at ambient pressure for30 minutes. islocation and a piece of liver weighing approximately 850 mg was Table Igives the mean values for liver oxygen consumption from emoved and the wet weight was recorded. The hepatic tissue was 16 animals. Astatistical analysis revealed that there were no signifi- hen homogenized in phosphate buffer for 15 seconds. A2-ml sample cant differences (P<0.1) inliver oxygen consumption between ani- f the liverhomogenate was placed in each of two 50-ml Erlenmeyer mals. The toxic effects of oxygen on oxidative metabolism were asks containing 22 of dextrose and 20 ml of mammalian Krebs- demonstrated by a 50.6% reduction in oxygen of the =mg consumption inger solution (pH 7.60, osmolarity 289.25 m Osm/Kg) modified experimental homogenates exposed to hyperbaric oxygen for 30 icontain 2.94 g NaHCOi. Inaddition, 78 mg of reduced glutathione minutes. This oxygen-induced reduction in oxygen consumption was was added to one of the flasks making the finalconcentration ofGSH significant at a P<0.001. n the flask 12 m M/l. Two 10-ml portions were then withdrawn from Control homogenates pretreated with GSH showed significantly oth Erlenmeyer flasks and each portion was placed in a separate higher (P<0.01) oxygen consumptions than controls containing no Academy Proceedings, XXXI, Arkansas of Science Vol. 1977 17 Published by Arkansas Academy of Science, 1977 17 Journal of the Arkansas Academy of Science, Vol. 31 [1977], Art. 6 D.A.Baeyens and M.J. Meier Table I: Oxygen consumption of mouse liver as influenced by creased production of ATP,but also utilizes more oxygen than other hyperbaric oxygen. Oxygen utilization determinations were made oxidative phosphorylation substrates. This explains the increased after 30 minutes at 20°C and were expressed in terms of 1of oxygen oxygen consumptions of liver homogenates treated with GSH in our consumed per hour per mg wet weight. experiments. The GSH increases succinate production by means of resulting oxygen consumption 0 CONSUMPTION the GSH-succinate shunt in increased 2 ''> the tissue tomaintain the energetic efficiency ofATP formation. TISSUE PREPARATION'INCUBATION GAS Mean x S.E. (N) CHANGF.*** by CONTROL P =756.7 mm Hr 63.25 + 2.86(48)* conclusion, hepatic oxygen N In the 50.6% reduction in LIVER HOMOGENATE 2 consumption in the experimental liver homogenates exposed to - + LIVER°HOMOGENATE V56 7 ™ «« "••»+».•«?« «-' hyperbaric oxygen indicates that oxygen can directly inactivate an 12HMGSH enzyme or enzymes of oxidative metabolism. The fact that liver P EXPERIMENTAL O,=3837.8 mm Hg 31.27 ~+ 1.94f48)** - homogenates pretreated with GSH demonstrated higher rates of 2 50 f LIVER HOMOGENATE ' ' oxygen consumption than the homogenates without GSH can be ex- EXPERIMENTAL P 9«-50 + 3.36(48) O,--'383Mi7'-18' mm HR +15.8 First, LIVER HOMOGENATE+ 2 "R plained by two different mechanisms. GSH protects the 12mM GSH J ] l enzymes of oxidative metabolism by maintaining them in a reduced ?Significantly different from CSH treatments at 13! level. and viable state.
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