DOCSLIB.ORG

Aislamiento De Salmonella Spp., Y Escherichia Coli Resistente a Las Β

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Aislamiento De Salmonella Spp., Y Escherichia Coli Resistente a Las Β UNIVERSIDAD CENTRAL DEL ECUADOR FACULTAD DE MEDICINA VETERINARIA Y ZOOTECNIA CARRERA DE MEDICINA VETERINARIA Y ZOOTECNIA Aislamiento de Salmonella spp., y Escherichia coli resistente a las β- Lactamasas (BLEE) en contenido cecal y ganglios mesentéricos en cerdos faenados en la Empresa Pública Metropolitana de Rastro Quito (EMRAQ-EP) Trabajo de titulación, modalidad proyecto de investigación para la obtención del Título de Médico Veterinario Zootecnista AUTOR: Cajas Romero Bryan Vicente TUTORA: MSC. PhD. María Belén Cevallos Almeida Quito, 2020 DERECHOS DEL AUTOR Yo, Bryan Vicente Cajas Romero en calidad de autor y titular de los derechos morales y patrimoniales del trabajo de titulación “Aislamiento de Salmonella spp., y Escherichia coli resistente a las β-lactamasas (BLEE) en contenido cecal y ganglios mesentéricos en cerdos faenados en la Empresa Pública Metropolitana de Rastro Quito (EMRAQ-EP)”, modalidad proyecto de investigación, de conformidad con el Art. 114 del CÓDIGO ORGÁNICO DE LA ECONOMÍA SOCIAL DE LOS CONOCIMIENTOS, CREATIVIDAD E INNOVACIÓN, concedo a favor de la Universidad Central del Ecuador una licencia gratuita, intransferible y no exclusiva para el uso no comercial de la obra, con fines estrictamente académicos. Conservo a mi favor todos los derechos de autor sobre la obra, establecidos en la normativa citada. Así mismo, autorizo a la Universidad Central del Ecuador para que realice la digitalización y publicación de este trabajo de titulación en el repositorio virtual, de conformidad a lo dispuesto en el Art. 144 de la Ley Orgánica de Educación Superior. El autor declara que la obra objeto de la presente autorización es original en su forma de expresión y no infringe el derecho de autor de terceros, asumiendo la responsabilidad por cualquier reclamación que pudiera presentarse por esta causa y liberando a la Universidad de toda responsabilidad. ---------------------------------- Bryan Vicente Cajas Romero C.I.: 171816108-4 e-mail: [email protected] ii APROBACIÓN DEL TUTOR Yo, María Belén Cevallos Almeida en calidad de tutor del trabajo de titulación, modalidad proyecto de investigación cuyo título es “Aislamiento de Salmonella spp., y Escherichia coli resistente a las β-lactamasas (BLEE) en contenido cecal y ganglios mesentéricos en cerdos faenados en la Empresa Pública Metropolitana de Rastro Quito (EMRAQ-EP)” elaborado por el estudiante Bryan Vicente Cajas Romero, para la obtención del título de Médico Veterinario y Zootecnista, considero que el mismo reúne los requisitos y méritos necesarios en el campo metodológico y en el campo epistemológico, para ser sometido a la evaluación por parte del jurado examinador que se designe, por lo que APRUEBO, a fin de que el trabajo sea habilitado para continuar con el proceso de titulación determinado por la Universidad Central del Ecuador. En la ciudad de Quito, a los 10 días del mes de septiembre del 2020. --------------------------------- Dra. María Belén Cevallos MSC. PhD. e-mail: [email protected] iii APROBACIÓN DEL INFORME FINAL/TRIBUNAL El tribunal constituido por: Presidente / Lector 1: Dra. María Inés Baquero. Lector 2: Dr. Christian Vinueza. Luego de calificar el informe final de investigación del trabajo de titulación denominado “Aislamiento de Salmonella spp., y Escherichia coli resistente a las β-lactamasas (BLEE) en contenido cecal y ganglios mesentéricos en cerdos faenados en la Empresa Pública Metropolitana de Rastro de Quito (EMRAQ-EP)” previo a la obtención del título de Médico Veterinario y Zootecnista, presentado por el señor Bryan Vicente Cajas Romero. Emite el siguiente veredicto: (aprobado/reprobado) / ordena que se hagan las siguientes correcciones: ……………………………………………………………………………………. Fecha: ……………………………………… Para constancia de lo actuado firman (se detallan las calificaciones en el caso de aprobación o reprobación, en el caso de ordenar correcciones, estas se detallan en un documento anexo y no se consiga la calificación en el párrafo que sigue). DOCENTE CALIFICACIÓN FIRMA Presidente / Lector 1 Dra. María Inés Baquero ……………………….. ………………….…... Lector 2 Dr. Christian Vinueza ……...……………..... ..…………………..… iv DEDICATORIA Con mucho cariño para los pilares fundamentales de mi vida, mis padres Marco Cajas y Mery Romero quienes me formaron como una gran persona y además me apoyaron tanto moralmente como económicamente durante toda mi vida estudiantil para poder cumplir mi sueño anhelado de ser un gran profesional. A mi hermano Marco quién con sus ocurrencias diarias y su ayuda constante me enseñó a ser una mejor persona. A mi novia Nathaly Paucar por confiar en mí y apoyarme de forma incondicional en todo el desarrollo de mi tesis. También para todos mis amig@s especialmente las pitufinas (Nathaly Chilla-Justina, Jessica Briones-La 5, Tania Riera-Molleja, Daysi Yánez-Pollito), Emilia Villagómez, Leydi Lema (Chiniz), Cristina García, Don Jorgito quienes me brindaron una grata amistad, un apoyo sincero y sobre todo sus grandes consejos. v AGRADECIMIENTOS A la Universidad Central del Ecuador, por haberme abierto sus puertas para realizar y cumplir mis estudios superiores. A la Facultad de Medicina Veterinaria y Zootecnia, la cual me acogió, me permitió conocer grandes amigos y lo más importante me brindo los conocimientos necesarios para culminar toda la carrera y desarrollarme como profesional. A la Empresa Publica Metropolitana de Rastro Quito (EMRAQ-EP) dirigida por el Dr. Ramiro Montesdeoca, el cual permitió el uso de las instalaciones durante la realización de mi proyecto de tesis y también a los operarios quienes siempre me colaboraron muy amablemente al momento de recolectar las muestras. Al Laboratorio de Bacteriología y Micología de la Facultad de Medicina Veterinaria y Zootecnia y a la Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR) por enseñarme, ayudarme y facilitarme el uso de sus instalaciones y sus equipos para desarrollar mi proyecto de pasantías y posteriormente mi tesis. A mi tutora de tesis, la Dra. María Belén Cevallos Almeida, gracias por haberme enseñado sus conocimientos en microbiología cuando fui estudiante y pasante. Además, le agradezco de corazón por su amabilidad, su gran paciencia y todo el apoyo brindado desde que inicio hasta que finalizo mi tesis. vi ÍNDICE GENERAL DERECHOS DEL AUTOR ............................................................................................ ii APROBACIÓN DEL TUTOR ........................................................................................ iii APROBACIÓN DEL INFORME FINAL/TRIBUNAL ..................................................... iv DEDICATORIA ............................................................................................................. v AGRADECIMIENTOS ................................................................................................. vi ÍNDICE GENERAL ...................................................................................................... vii LISTADO DE TABLAS ................................................................................................ ix LISTADO DE FIGURAS ............................................................................................... x LISTADO DE GRÁFICOS ........................................................................................... xi LISTADO DE ANEXOS ............................................................................................... xii RESUMEN .................................................................................................................. xiii ABSTRACT ................................................................................................................ xiv CAPITULO I ................................................................................................................. 1 INTRODUCCIÓN .......................................................................................................... 1 CAPITULO II ................................................................................................................ 4 OBJETIVOS ................................................................................................................. 4 OBJETIVO GENERAL .............................................................................................. 4 OBJETIVOS ESPECÍFICOS ..................................................................................... 4 CAPITULO III ............................................................................................................... 5 REVISIÓN BIBLIOGRÁFICA ....................................................................................... 5 Salmonella ................................................................................................................... 5 Historia ..................................................................................................................... 5 Taxonomía ............................................................................................................... 5 Características Bacteriológicas ............................................................................. 6 Características Antigénicas ................................................................................... 7 Epidemiología .......................................................................................................... 7 Factores de virulencia ............................................................................................ 8 Colonización y Patogenicidad
Load more

Recommended publications
  • Escherichia Coli and Shigella Species M
    INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Apr. 1988, p. 201-206 Vol. 38. No. 2 0020-7713/88/040201-06$02.OO/O Specificity of a Monoclonal Antibody for Alkaline Phosphatase in Escherichia coli and Shigella Species M. 0. HUSSON,1*2*P. A. TRINELY2C. MIELCAREK,2 F. GAVINI,2 C. CARON,lq2D. IZARD,l AND H. LECLERC' Faculte' de Me'decine, Laboratoire de Bacte'riologie A, 59045 Lille Cedex, France' and Unite' Institut National de la Sante' et de la Recherche Me'dicale 146, Domaine dir CERTIA, 59650 Villeneuve-d'Ascq Cedex, France2 The specificity of monoclonal antibody 2E5 for the alkaline phosphatase of Escherichia coli was studied against the alkaline phosphatases of 251 other bacterial strains. The organisms used included members of the six species of the genus Escherichia (E. coli, E. fergusonii, E. hermannii, E. blattae, E. vulneris, E. adecarboxylata), 41 species representing the family Enterobacteriaceae, and, in addition, Pseudomonas aeruginosa, Aeromonas spp., Plesiomonas shigelloides, Acinetobacter calcoaceticus, and Vibrio cholerae non-01. Three methods were used. An enzyme-linked immunosorbent assay was performed against 21U of alkaline phosphatase per ml; immunofluorescence against bacterial cells and Western blotting against periplasmic proteins were also used. All of our experiments demonstrated the high specificity of monoclonal antibody 2E5. This antibody recognized only E. coli (118 strains tested) and the four species of the genus Shigella (S. sonnei, S. flexneri, S. boydii, S. dysenteriae; 12 strains tested). Since the description of hybridoma production by Kohler mouse immunized with purified Escherichia coli ATCC and Milstein in 1975 (14), many monoclonal antibodies 10536 alkaline phosphatase as described elsewhere (12).
    [Show full text]
  • International Journal of Systematic and Evolutionary Microbiology (2016), 66, 5575–5599 DOI 10.1099/Ijsem.0.001485
    International Journal of Systematic and Evolutionary Microbiology (2016), 66, 5575–5599 DOI 10.1099/ijsem.0.001485 Genome-based phylogeny and taxonomy of the ‘Enterobacteriales’: proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Mobolaji Adeolu,† Seema Alnajar,† Sohail Naushad and Radhey S. Gupta Correspondence Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Radhey S. Gupta L8N 3Z5, Canada [email protected] Understanding of the phylogeny and interrelationships of the genera within the order ‘Enterobacteriales’ has proven difficult using the 16S rRNA gene and other single-gene or limited multi-gene approaches. In this work, we have completed comprehensive comparative genomic analyses of the members of the order ‘Enterobacteriales’ which includes phylogenetic reconstructions based on 1548 core proteins, 53 ribosomal proteins and four multilocus sequence analysis proteins, as well as examining the overall genome similarity amongst the members of this order. The results of these analyses all support the existence of seven distinct monophyletic groups of genera within the order ‘Enterobacteriales’. In parallel, our analyses of protein sequences from the ‘Enterobacteriales’ genomes have identified numerous molecular characteristics in the forms of conserved signature insertions/deletions, which are specifically shared by the members of the identified clades and independently support their monophyly and distinctness. Many of these groupings, either in part or in whole, have been recognized in previous evolutionary studies, but have not been consistently resolved as monophyletic entities in 16S rRNA gene trees. The work presented here represents the first comprehensive, genome- scale taxonomic analysis of the entirety of the order ‘Enterobacteriales’.
    [Show full text]
  • Intrahepatic Bacterial Metataxonomic Signature in Non-Alcoholic Fatty Liver
    Hepatology ORIGINAL RESEARCH Intrahepatic bacterial metataxonomic signature in Gut: first published as 10.1136/gutjnl-2019-318811 on 2 January 2020. Downloaded from non- alcoholic fatty liver disease Silvia Sookoian ,1,2 Adrian Salatino,1,3 Gustavo Osvaldo Castaño,4 Maria Silvia Landa,1,3 Cinthia Fijalkowky,1,3 Martin Garaycoechea,5 Carlos Jose Pirola 1,3 ► Additional material is ABSTRact published online only. To view Objective We aimed to characterise the liver tissue Significance of this study please visit the journal online bacterial metataxonomic signature in two independent (http:// dx. doi. org/ 10. 1136/ What is already known on this subject? gutjnl- 2019- 318811). cohorts of patients with biopsy- proven non- alcoholic fatty liver disease (NAFLD) diagnosis, as differences in ► The natural history of non- alcoholic fatty liver For numbered affiliations see disease (NAFLD) is modulated by genetic and end of article. the host phenotypic features—from moderate to severe obesity—may be associated with significant changes in environmental factors. ► Recent discoveries revealed the role of the Correspondence to the microbial DNA profile. Dr Silvia Sookoian, Institute Design and methods Liver tissue samples from 116 gut microbiota in human health and disease, of Medical Research A Lanari, individuals, comprising of 47 NAFLD overweight or including NAFLD. However, the impact of the University of Buenos Aires moderately obese patients, 50 NAFLD morbidly obese liver tissue microbial DNA profiling on the Faculty of Medicine, Buenos disease biology remains unknown. Aires, 10109 CABA, Argentina; patients elected for bariatric surgery and 19 controls, ssookoian@ intramed. net were analysed using high- throughput 16S rRNA gene What are the new findings? Dr Carlos Jose Pirola; sequencing.
    [Show full text]
  • Bacterial Bloodstream Infections in a Tertiary Infectious Diseases Hospital
    Dat et al. BMC Infectious Diseases (2017) 17:493 DOI 10.1186/s12879-017-2582-7 RESEARCHARTICLE Open Access Bacterial bloodstream infections in a tertiary infectious diseases hospital in Northern Vietnam: aetiology, drug resistance, and treatment outcome Vu Quoc Dat1,2,3* , Hieu Ngoc Vu1, Hung Nguyen The1, Hoa Thi Nguyen1, Long Bao Hoang3, Dung Vu Tien Viet3, Chi Linh Bui1, Kinh Van Nguyen2, Trung Vu Nguyen1,2, Dao Tuyet Trinh2, Alessandro Torre3, H. Rogier van Doorn3,4, Behzad Nadjm3,4 and Heiman F.L. Wertheim3,4,5 Abstract Background: Bloodstream infections (BSIs) are associated with high morbidity and mortality worldwide. However their aetiology, antimicrobial susceptibilities and associated outcomes differ between developed and developing countries. Systematic data from Vietnam are scarce. Here we present aetiologic data on BSI in adults admitted to a large tertiary referral hospital for infectious diseases in Hanoi, Vietnam. Methods: A retrospective study was conducted at the National Hospital for Tropical Diseases between January 2011 and December 2013. Cases of BSI were determined from records in the microbiology department. Case records were obtained where possible and clinical findings, treatment and outcome were recorded. BSI were classified as community acquired if the blood sample was drawn ≤48 h after hospitalization or hospital acquired if >48 h. Results: A total of 738 patients with BSI were included for microbiological analysis. The predominant pathogens were: Klebsiella pneumoniae (17.5%), Escherichia coli (17.3%), Staphylococcus aureus (14.9%), Stenotrophomonas maltophilia (9.6%) and Streptococcus suis (7.6%). The overall proportion of extended spectrum beta-lactamase (ESBL) production among Enterobacteriaceae was 25.1% (67/267 isolates) and of methicillin-resistance in S.
    [Show full text]
  • Update on Modern Bacterial Taxonomy for Bench Microbiologists
    11/5/2018 Update on Modern Bacterial Taxonomy for Bench Microbiologists J. Michael Janda Kern County Public Health Laboratory Bakersfield CA The “Name Game” Which Ones Different? Bacterial Taxonomy for Why is Taxonomy Important? Clinical Microbiologists • Think of it as a “LABEL” NOMENCLATURE CLASSIFICATION • We communicate with colleagues around the world regarding • Correct naming and use of • Determines where specific infectious diseases using a standardized communication bacterial names species or groups reside system, microbial taxonomy • Codified compared to others • Basic rules are in • Determines • We describe new diseases, disease outbreaks, epidemics, or pandemics, and emerging syndromes using a binomial name the “Red Book” phylogenetic • to indicate the infectious agent(s) Unlike classification, position relatively stable • Not codified • A lack of a standardized communication system can lead to: but subject to • Can change rapidly – Misidentifications change • What is accepted is – Inappropriate therapeutic decisions based upon common – Inability to determine disease prognosis, treatment and pathogenicity usage and acceptance by the – Identify important outbreaks and epidemics scientific community 1 11/5/2018 Description of a New Species [Idealized] Some Requirements for a Valid Species • Type strain must be identified and defined (etymology); deposited Bios in at least two recognized international culture collections Morpho & 16S • Properties of the taxon, including phenotypic characteristics must Physio NEW be described and compared to closest neighbors on both a phylogenetic as swell as phenotypic basis SPECIES – Cell shape, motility, color, Gram stain DDH, Chemo ANI – Physiologic properties: pH, temperature range Type • Strain G+C mol% • Full 16S rRNA gene sequence, DDH, and/or ANI Classification Pathways to Species Validation Enterobacter Cronobacter Proposed New sakazakii sakazakii Species (Farmer, 1980) (Iversen, 2008) C.
    [Show full text]
  • A Review of the Taxonomy, Genetics and Biology of the Genus Escherichia and the Type Species Escherichia Coli
    Canadian Journal of Microbiology A Review of the Taxonomy, Genetics and Biology of the Genus Escherichia and the Type Species Escherichia coli Journal: Canadian Journal of Microbiology Manuscript ID cjm-2020-0508.R1 Manuscript Type: Review Date Submitted by the 13-Feb-2021 Author: Complete List of Authors: Yu, Daniel ; University of Alberta School of Public Health, Room 357 South Academic Bldg, University of Alberta Banting, Graham; University of Alberta School of Public Health, Room 357 South Academic Bldg, University of Alberta Neumann, DraftNorman; University of Alberta School of Public Health, Room 357 South Academic Bldg, University of Alberta; Keyword: Escherichia coli, taxonomy, host specificity, ecotype, genetics Is the invited manuscript for consideration in a Special Not applicable (regular submission) Issue? : © The Author(s) or their Institution(s) Page 1 of 67 Canadian Journal of Microbiology A Review of the Taxonomy, Genetics and Biology of the Genus Escherichia and the Type Species Escherichia coli Daniel Yu1, Graham Banting1, and Norman F. Neumann1* 1School of Public Health, University of Alberta, Edmonton, Alberta, Canada, T6G IC9 Draft * Corresponding Author: Dr. Norman F. Neumann Professor and Vice Dean School of Public Health Room 3-381 Edmonton Clinic Health Academy University of Alberta Edmonton, Alberta, Canada. T6G 1C9 Email: [email protected] 1 © The Author(s) or their Institution(s) Canadian Journal of Microbiology Page 2 of 67 ABSTRACT Historically, bacteriologists have relied heavily on biochemical and structural phenotypes for bacterial taxonomic classification. However, advances in comparative genomics has led to greater insights into the remarkable genetic diversity within the microbial world, and even within well-accepted species such as Escherichia coli.
    [Show full text]
  • TYGS Is an Automated High-Throughput Platform for State-Of-The-Art Genome-Based Taxonomy
    ARTICLE https://doi.org/10.1038/s41467-019-10210-3 OPEN TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy Jan P. Meier-Kolthoff 1 & Markus Göker 1 Microbial taxonomy is increasingly influenced by genome-based computational methods. Yet such analyses can be complex and require expert knowledge. Here we introduce TYGS, the Type (Strain) Genome Server, a user-friendly high-throughput web server for genome-based 1234567890():,; prokaryote taxonomy, connected to a large, continuously growing database of genomic, taxonomic and nomenclatural information. It infers genome-scale phylogenies and state-of- the-art estimates for species and subspecies boundaries from user-defined and automatically determined closest type genome sequences. TYGS also provides comprehensive access to nomenclature, synonymy and associated taxonomic literature. Clinically important examples demonstrate how TYGS can yield new insights into microbial classification, such as evidence for a species-level separation of previously proposed subspecies of Salmonella enterica. TYGS is an integrated approach for the classification of microbes that unlocks novel scientific approaches to microbiologists worldwide and is particularly helpful for the rapidly expanding field of genome-based taxonomic descriptions of new genera, species or subspecies. 1 Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany. Correspondence and requests for materials should be addressed to J.P.M.-K. (email: [email protected]) NATURE COMMUNICATIONS | (2019) 10:2182 | https://doi.org/10.1038/s41467-019-10210-3 | www.nature.com/naturecommunications 1 ARTICLE NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-019-10210-3 arth is dominated by microbes as these regulate and sustain species are published, recently made it mandatory for such Elife in various ways1,2.
    [Show full text]
  • 206494Orig1s000
    CENTER FOR DRUG EVALUATION AND RESEARCH APPLICATION NUMBER: 206494Orig1s000 STATISTICAL REVIEW(S) NDA 206494 Ceftazidime-Avibactam MEMORANDUM Date 2/11/2015 From Thamban Valappil, PhD, Statistical Team Leader NDA#: 206494 Drug Name: Ceftazidime-Avibactam Applicant: AztraZeneca-Forest-Cerexa Indication(s): Complicated intra-abdominal infections (cIAI) and Complicated urinary tract infections (cUTI) Submission Date: 25 June, 2014 PDUFA due date: 25 February, 2015 Biometrics Division DB4 Concurring Reviewer(s) Dionne Price, PhD, Division Director Primary Statistical Reviewer: Margaret Gamalo-Siebers, PhD Clinical Reviewer: Benjamin Lorenz, MD Project Manager: Carmen DeBellas, PharmD Subject : Secondary Statistical Review and Evaluation Reference : Primary Statistical Review, and other Agency communications 1. Introduction and background This secondary review is intended to summarize and highlight some of the findings in the primary statistical review of Dr. Gamalo-Siebers and includes some additional comments. In the statistical review, Dr. Gamalo-Siebers included a set of novel statistical approaches that warrant further exploration in the future. The findings from those exploratory analyses have not been included in this memorandum. Reference ID: 3701514 NDA 206494 Ceftazidime-Avibactam The new drug application (NDA) 206494 evaluates ceftazidime-avibactam (CAZ-AVI), which is a combination of ceftazidime, a third-generation cephalosporin antibacterial drug, and avibactam, a non-β-lactam, β-lactamase inhibitor (BLI). According to the Applicant,
    [Show full text]
  • Genus I. Escherichia Castellani and Chalmers 1919, 941TAL
    FAMILY I. ENTEROBACTERIACEAE 607 kazakii in most biochemical reactions, including production of a by Kosako et al. (1996) indicated that Enterobacter kobei is the yellow pigment. However, they were only 43% related to E. sa- closest relative of Enteric Group 69. kazakii by DNA–DNA hybridization. Recent DNA relatedness data Genus I. Escherichia Castellani and Chalmers 1919, 941TAL FLEMMING SCHEUTZ AND NANCY A. STROCKBINE Esch.er.iЈchi.a. M.L. fem. n. Escherichia named after Theodor Escherich, who isolated the type species of the genus. -lm, occurring singly cannot be used by E. coli and E. vulneris, whereas a smaller pro 6.0–2.0 ן Straight cylindrical rods, 1.1–1.5 or in pairs. Conform to the general definition of the family En- portion of E. fergusonii and E. hermannii exhibit immediate or terobacteriaceae. Gram negative. Motile by peritrichous flagella or delayed use of this substrate. Growth on Simmons’ citrate agar nonmotile. Aerobic and facultatively anaerobic having both a by E. blattae is variable and probably strain specific. respiratory and a fermentative type of metabolism, but anaero- Lysine is decarboxylated by the majority of strains. Exceptions genic biotypes occur. Oxidase negative. Chemoorganotrophic. include “metabolically inactive” E. coli strains, the majority of Both acid and gas are formed from most fermentable carbo- enteroinvasive E. coli strains (EIEC), and E. hermannii. Ornithine hydrates, but i-inositol is not utilized and d-adonitol is utilized is decarboxylated by all species except by E. vulneris and a little only by Escherichia fergusonii. Lactose is fermented by most strains less than half of E.
    [Show full text]
  • Tese De Doutorado
    Universidade Federal do Rio Grande do Sul Instituto de Ciência e Tecnologia de Alimentos Programa de Pós-graduação em Ciência e Tecnologia de Alimentos (PPGCTA) TESE DE DOUTORADO Glória Alberto Manhique AVALIAÇÃO DAS CONDIÇÕES HIGIÊNICO-SANITÁRIAS E CONTAMINAÇÃO MICROBIOLÓGICA DE ALIMENTOS, MANIPULADORES E UTENSÍLIOS UTILIZADOS NA PREPARAÇÃO DE ALIMENTOS EM MERCADOS E NAS RUAS DE MAPUTO, MOÇAMBIQUE. Porto Alegre Novembro, 2020 Universidade Federal do Rio Grande do Sul Instituto de Ciência e Tecnologia de Alimentos Programa de Pós-graduação em Ciência e Tecnologia de Alimentos (PPGCTA) GLÓRIA ALBERTO MANHIQUE BIÓLOGA – UPM/FCNM MESTRE EM CIÊNCIA E EDUCAÇÃO AGRÁRIA – UEM/FAEF TEMA: AVALIAÇÃO DAS CONDIÇÕES HIGIÊNICO-SANITÁRIAS E CONTAMINAÇÃO MICROBIOLÓGICA DE ALIMENTOS, MANIPULADORES E UTENSÍLIOS UTILIZADOS NA PREPARAÇÃO DE ALIMENTOS EM MERCADOS E NAS RUAS DE MAPUTO, MOÇAMBIQUE. Orientador: Prof. Dr. Eduardo Cesar Tondo Tese apresentada ao Programa de Pós- Graduação em Ciência e Tecnologia de Alimentos (Área de Concentração Ciência e Tecnologia de alimentos), como requisito para obtenção do Grau de Doutor em Ciência e Tecnologia de Alimentos. Porto Alegre Novembro, 2020 iii iv Tese de Doutorado Avaliação das condições higiênico-sanitárias e contaminação microbiológica de alimentos, manipuladores e utensílios utilizados na preparação de alimentos em mercados e nas ruas de Maputo, Moçambique. Glória Alberto Manhique Tese apresentada ao Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos (PPGCTA) - Universidade Federal do Rio Grande
    [Show full text]
  • C G M 2 0 1 8 [0 4 on D Er Z O E K S R a Pp O
    Update of the bacterial the of bacterial Update intaxonomy the classification lists of COGEM CGM 2018 - 04 ONDERZOEKSRAPPORT report Update of the bacterial taxonomy in the classification lists of COGEM July 2018 COGEM Report CGM 2018-04 Patrick L.J. RÜDELSHEIM & Pascale VAN ROOIJ PERSEUS BVBA Ordering information COGEM report No CGM 2018-04 E-mail: [email protected] Phone: +31-30-274 2777 Postal address: Netherlands Commission on Genetic Modification (COGEM), P.O. Box 578, 3720 AN Bilthoven, The Netherlands Internet Download as pdf-file: http://www.cogem.net → publications → research reports When ordering this report (free of charge), please mention title and number. Advisory Committee The authors gratefully acknowledge the members of the Advisory Committee for the valuable discussions and patience. Chair: Prof. dr. J.P.M. van Putten (Chair of the Medical Veterinary subcommittee of COGEM, Utrecht University) Members: Prof. dr. J.E. Degener (Member of the Medical Veterinary subcommittee of COGEM, University Medical Centre Groningen) Prof. dr. ir. J.D. van Elsas (Member of the Agriculture subcommittee of COGEM, University of Groningen) Dr. Lisette van der Knaap (COGEM-secretariat) Astrid Schulting (COGEM-secretariat) Disclaimer This report was commissioned by COGEM. The contents of this publication are the sole responsibility of the authors and may in no way be taken to represent the views of COGEM. Dit rapport is samengesteld in opdracht van de COGEM. De meningen die in het rapport worden weergegeven, zijn die van de auteurs en weerspiegelen niet noodzakelijkerwijs de mening van de COGEM. 2 | 24 Foreword COGEM advises the Dutch government on classifications of bacteria, and publishes listings of pathogenic and non-pathogenic bacteria that are updated regularly.
    [Show full text]
  • Enterobacteria in the 21St Century: a Review Focused on Taxonomic Changes
    Review Enterobacteria in the 21st century: a review focused on taxonomic changes 1 2 1 1 Soraya Morales-López , Jayr A Yepes , Juan C Prada-Herrera , Augusto Torres-Jiménez 1 CINBIOS Research Group, Department of Microbiology, Universidad Popular del Cesar, Valledupar, Colombia 2 Research Group of Biotechnology and Enviromental Genotoxicity, Department of Microbiology, Universidad Popular del Cesar, Valledupar, Colombia Abstract Introduction: Enterobacteria are the main group causing infections in humans. The aim of this review is to present the new genera and the taxonomic changes that the Enterobacteriacea family has experienced in recent years. Methodology: a systematic search of papers published in databases from January 2000 to July 2018 was done. Additionally, the bibliographic references of each document were reviewed and each paper citing the article was reviewed in search of clinical cases. Results: Nineteen new genera of Enterobacteria have been described since 2000. The genera Yersinia, Morganella and Erwinia do not belong to the family Enterobacteriacea anymore. Conclusions: for an adequate clinical and epidemiological interpretation, it is advisable to update the libraries of the commercial systems used for the identification of the microorganisms, as well as to train the staff in the taxonomic changes of microorganisms. Key words: Enterobacteriaceae; Escherichia; Enterobacterales; Morganellaceae. J Infect Dev Ctries 2019; 13(4):265-273. doi:10.3855/jidc.11216 (Received 09 January 2019 – Accepted 23 January 2019) Copyright © 2019 Morales-López et al. This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
    [Show full text]