Monoclonal Mouse Anti-Human Cd79acy

Monoclonal Mouse Anti-Human CD79cy/APC, Clone HM57 Code C7252 Monoclonal Mouse Anti-Human CD79cy/RPE, Clone HM57 Code R7159

Analyte specific reagent. Analytical and performance characteristics are not established.

Summary and explanation CD79 is a disulphide-linked transmembrane heterodimer with a molecular mass of 82-95 kDa belonging to the immunoglobulin superfamily. It comprises the two glycoproteins, CD79 with a molecular mass of 40-45 kDa, and CD79 with a molecular mass of 37 kDa. CD79 is non-covalently associated with surface Ig, forming the B-cell receptor complex, which is required for antigen recognition. In precursor B cells, the CD79 protein chains are already expressed in the cytoplasm (CyCD79). Surface expression of CD79 begins at the pro-B cell stage and persists throughout the B-cell differentiation. CD79 is more strongly expressed by B cells in the follicular mantle zone than by germinal centre B cells, suggesting that activation of mature B cells downregulates CD79 expression. CD79 expression ceases around the onset of plasma cell differentiation, with only a proportion of plasma cells containing CD79 (1, 2). The HM57 antibody was clustered as Anti-CD79αcy at the Fifth International Workshop on Human Leucocyte Differentiation Antigens (3).

Reagent provided C7252 is a purified monoclonal mouse antibody conjugated with allophycocyanin (APC). R7159 is a purified monoclonal mouse antibody conjugated with R-phycoerythrin (RPE). The conjugates are provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 15 mmol/L NaN3, pH 7.2. Isotype: IgG1, kappa. Conjugate concentration mg/L: See label on vial.

Immunogen A synthetic peptide, corresponding to amino acids 202-216 from the CD79 protein (4).

Precautions 1. Analyte specific reagent. Analytical and performance characteristics are not established. 2. For professional users.

3. This product contains sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 4. As with any product derived from biological sources, proper handling procedures should be used. 5. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 6. Unused solution should be disposed of according to local, State and Federal regulations.

Storage Store in the dark at 2-8 C. Do not use after expiration date stamped on vial. If reagents are stored under any conditions other than those specified, the conditions must be verified by the user. There are no obvious signs to indicate instability of this product. If unexpected staining is observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Dako Technical Support.

References 1. Comans-Bitter WM, De Bruin-Versteeg S, Broe MK, De Groot R, De Vries E, Van Dongen JJM. BC20.1. CD79 workshop: intracellular CD79 expression in precursor B cells tested with the CD79 panel of monoclonal antibodies. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 182-4. 2. Nakamura T. BC20. CD79 workshop panel report. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 180-2. 3. Engel P, Wagner N, Tedder TF. B23. CD79 workshop report. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al., editors. Leucocyte typing V. White cell differentiation antigens. Proceedings of the 5th International Workshop and Conference; 1993 Nov 3-7; Boston, USA. Oxford, New York, Tokyo: Oxford University Press; 1995. Volume 1. p. 667-70. 4. Mason DY, Cordell JL, Tse AGD, van Dongen JJM, van Noesel JM, Micklem K, et al. The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol 1991;147:2474-82.

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Revision 2019.02

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