DOCSLIB.ORG

Effects of Nitrogen Supplementation on Saccharomyces Cerevisiae JP14

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Effects of Nitrogen Supplementation on Saccharomyces Cerevisiae JP14 a OSSN 0101-2061 (Print) OSSN 1678-457X (Dnline) Food Science and Technology DDO: https://doi.org/10.1590/fst.11219 Effects of nitrogen supplementation on Saccharomyces cerevisiae JP14 fermentation for mead production Eduardo Luís Menezes de ALMEODA1, Gustavo MDREORA E SOLVA2, Ogor de Albuquerque VASSALLO2, Mayara Salgado SOLVA3, Weyder Cristiano Santana4, Paulo Henrique Alves da SOLVA1, Monique Renon ELLER1* Abstract Honey must supplementation is necessary for mead production due to the deficiency in nitrogen materials in this feedstock, despite its high fermentative sugar content. The nitrogen limitation can halt or slow fermentation and lead to the production of unpleasant sensorial compounds, such as sulfur derivatives. The yeast JP14, aSaccharomyces cerevisiae isolated from Jataí bee’s pollen, was inoculated in 25 °Brix honey must with – 0 (control); 0,3; 0,7 and 1,0 g∙L-1 – of di-ammonium phosphate (DAP) and ammonium sulfate (AS). The addition of both supplements resulted in increased cell viability in the first 5 days of fermentation at 20 °C, but did not affect the final acidity of the produced meads. Supplementation also leads to increased sugar consumption, and sugar conversion into ethanol increased as nitrogen supplementation increased, especially with DAP. This indicates that these compounds also regulate yeast metabolic pathways. Supplementary nitrogen acts both in protein anabolism and the gene expression of glycolytic and fermentative pathway components, favoring, in this case, sugar conversion into ethanol. This is the first work describing how different DAP and AS concentrations influences mead production and showing the comparison between these two supplements. Keywords: yeasts; beverages; ammonium; additives. Practical Application: Dptimization of mead production. 1 Introduction On nature, yeasts are capable of using a wide range of compounds Ammonium sulfate (AS) and di-ammonium phosphate (DAP) containing nitrogen. Some of these compounds are metabolized are efficient alternatives for fermentation in sugarcane must, more efficiently and optimize growth and the metabolic activity, Vidal et al. (2013), grape must, Childs et al. (2015) and apple, Barbosa et al. (2012). During the fermentative process, nitrogen Kelkar & Dolan (2012). Mendes-Ferreira et al. (2010) evaluated limitation can halt or slow fermentation, besides stimulating the DAP supplementation together with acids, but, to the present production of unpleasant sensorial compounds, such as sulfur date, no works were found with only DAP addition compared derivatives, Mendes-Ferreira et al. (2004). to AS, or the determination of its respective optimal points, as Mead is considered the oldest known alcoholic beverage supplements for honey must. The use of these sources must be Aquarone et al. (1983). However, mead production is not evaluated specifically for each must and the fermentation agent standardized and techniques and ingredients from winemaking are utilized, in order to obtain the ideal type and concentration of often used. However, using different feedstocks requires different these agents for each beverage to be produced. Therefore, this upstream operations, including honey must supplementation, work aims to study ammonium sulfate (AS) and di-ammonium which is a feedstock deficient in nitrogen sources. phosphate (DAP) as sources for honey-based must for mead production by yeast JP14. On search for new yeasts for mead fermentation, our research group isolated and identified yeasts from different apicultural 2 Materials and methods sources and selected them for their fermentative capacity. Among the isolated yeasts, the strain JP14, identified as aSaccharomyces 2.1 Pre-inoculum preparation and yeast stock maintenance cerevisiae, stands out for its great potential to produce mead and The yeast JP14 was obtained from the microorganism bank other beverages. However, fermentative process scheduling led of the Laboratory of Biochemical and Fermentative Processes to productivity problems that could affect the industrial scale of the Department of Food Technology of the Universidade application viability. Federal de Viçosa. The yeast was propagated in YEPG medium Must supplementation with nitrogen sources reduces the [0,5% (w/v) yeast extract - KASVO, 1% (w/v) peptone - OMEDOA harmful effects from low nutrient availability in feedstock. e 2% (w/v) glucose - NEDN] at 30 °C until 107 cel∙mL-1 count. Received 08 May, 2019 Accepted 23 Oct., 2019 1 Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa – UFV, Viçosa, MG, Brasil 2 Departmento de Química, Universidade Federal de Viçosa – UFV, Viçosa, MG, Brasil 3 Instituto Federal do Ceará, Campus Limoeiro do Norte, Limoeiro de Norte, CE, Brasil 4 Departmento de Entomologia, Universidade Federal de Viçosa – UFV, Viçosa, MG, Brasil *Corresponding author: [email protected], [email protected] 336 336/343 Food Sci. Technol, Campinas, 40(Suppl. 1): 336-343, June 2020 Almeida et al. The pre-inoculum was centrifuged at 3370x g for 10 min and 300 μL of DNS reagent, Sumner (1921); Vasconcelos et al. resuspended in 50 mL of must with 5% (w/v) honey containing (2013). The mixture was agitated and incubated in water bath 0.015% (w/v) sodium metabisulfite (Ondupropil). After 12 h at 90 °C for 10 min. After cooling, 1.6 mL of distillated water of adaptation, at 30 °C without agitation, it was transferred were added, and the absorbance reading was conducted in a to Erlenmeyers with 25 °Brix honey must. The pre-inoculum spectrophotometer at 540 nm. Distillated water was used to was incubated for 24 h at 30 °C and 150 rpm until 108 cel∙mL-1 replace sample aliquots for blanking. A standardized curve count, utilized for must inoculation (item 2.2.) at 106 cel∙mL-1. was generated to estimate the reducing sugars present in the Silva (2016) samples, using glucose at concentrations between 0 and 2.0 g∙L-1. The yeast JP14 was maintained at -15 °C in YEPG medium The samples were diluted when necessary. containing 20% (w/v) glycerol until the moment of its use. The ethanol concentration was determined through High Performance Liquid Chromatography. For such, aliquots 2.2 Must preparation and inoculation of 1 mL of each sample were centrifuged at 2.236 x g and the supernatant was frozen at -20 °C until the moment the Six different musts were prepared, besides one control, in analysis was performed. Later, the samples were injected in triplicate, which totaled 21 fermentation vessels. Three musts an Aminex HPX-87H column with sulfuric acid at 5 mM as were supplemented with 0.3; 0.7 and 1.0 g∙L-1 of di-ammonium mobile phase (VETEC) and identified by a refraction index phosphate (DAP), while the other three received ammonium detector. The ethanol concentrations used for the preparation sulfate (AS) at the same concentrations. The control treatment of the standard curve were 3, 5, 10, 25, 50, 75, 100 and 150 mM. corresponded to the must without any nitrogen supplementary The peak area obtained from the chromatogram was used to sources. determine the ethanol concentration in the samples. On must preparation, honey was diluted in sterile mineral water until 25 °Brix and added of 0.015% (w/v) of sodium The free amino nitrogen (FAN) was determined by the metabisulfite. Next, they were supplemented with the nitrogen spectrophotometric method, Abernathy et al. (2009), using dye sources, as mentioned above. The musts were pasteurized at reagent ninidrine 2% in glycol etilene/sodium acetate (pH 5,5) 65 °C for 30 min, immediately cooled, and then inoculated with buffer and glycine to prepare a standard curve. The absorbance the yeast JP14 at approximately 106 cel∙mL-1. The system was of each reaction was measured at 575 nm. homogenized and assembled using airlocks to allow gas exhaust and sampling, so as to prevent oxygen entrance in the system. 2.5 Statistical analysis The inoculated musts were maintained at 20 °C for fermentation. The experiments produced in this work were arranged in a completely randomized design, with three replications. Data 2.3 Monitoring of the fermentative process analysis was conducted with the aid of the ASSOSTAT software During the seven first days of fermentation (tumultuous system, Silva & Azevedo (2016). An analysis of variance was fermentation), cell viability, total soluble solids, reducing sugar, carried out between the samples using the F test at a 5% acid content, turbidity (Dptical Density – D. D., at 600 nm) probability level. When differences were detected, the Tukey test and free amino nitrogen (FAN) were monitored every 24 h. was conducted, at the same probability level, for the comparison After the seven first days, the analysis were carried out every between different nitrogen sources. The evaluation of the effect seven days, until day 28. The liquids were transferred to new of different concentrations for the same supplementary source flasks and cooled at 10 °C for the stabilization (maturation) was analyzed through a regression analysis using ANDVA. of the product. On the end of 30 d, the samples were analyzed for ethanol quantification through High Performance Liquid 3 Results Chromatography (HPLC). Finally, 50% (w/v) of the clarifying The meads produced with DAP and AS showed significant agent bentonite (Ondupropil) was added. After 48 h, the samples differences between them and when compared to the control were transferred to new flasks, and the mead production process (Table 1). Nitrogen supplementation leads to musts with similar was concluded. FAN and reducing sugar concentrations, but supplementation with DAP leads to more efficiency in sugar conversion into ethanol by 2.4 Analytical methods the yeast. However, the increase in FAN content did not result The cell concentration was calculated based on viable cell in a significant difference in final acidity and viable cell count. counting in Neubauer Chamber using methylene blue vital Supplementations with both DAP and AS increased cell viability dye, Pierce (1970). The content of soluble solids (°Brix) was (Figure 1A and B) in musts until the third fermentation day, determined with the aid of an analogic refractometer (ATC).
Load more

Recommended publications
  • Free Amino Nitrogen in Brewing
    fermentation Review Free Amino Nitrogen in Brewing Annie E. Hill * and Graham G. Stewart International Centre for Brewing & Distilling, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland; [email protected] * Correspondence: [email protected]; Tel.: +44-1314513458 Received: 22 January 2019; Accepted: 13 February 2019; Published: 18 February 2019 Abstract: The role of nitrogenous components in malt and wort during the production of beer has long been recognized. The concentration and range of wort amino acids impact on ethanolic fermentation by yeast and on the production of a range of flavour and aroma compounds in the final beer. This review summarizes research on Free Amino Nitrogen (FAN) within brewing, including various methods of analysis. Keywords: brewing; fermentation; free amino nitrogen; wort; yeast 1. Introduction The earliest written account of brewing dates from Mesopotamian times [1]. However, our understanding of the connection with yeast is relatively recent, starting with Leeuwenhoek’s microscope observations in the 17th century followed by the work of Lavoisier, Gay-Lussac, Schwann and others during the 18th and 19th centuries. It was not until the late 19th century that Pasteur demonstrated that fermented beverages result from the action of living yeast’s transformation of glucose (and other sugars) into ethanol [2–4]. Since then, our knowledge has expanded exponentially, particularly with the development of molecular biology techniques [5]. In this review, we cover the particular contribution that wort nitrogen components play in beer production during fermentation. A number of terms are used to define wort nitrogenous components: Free Amino Nitrogen (FAN) is a measure of the nitrogen compounds that may be assimilated or metabolised by yeast during fermentation.
    [Show full text]
  • Solubleproteins and Free Amino Nitrogen Content in Must and Wine of Cv
    Vitis37(3), 139-142(1998) Solubleproteins and free amino nitrogen content in must and wine of cv. Viura in La Rioja by FERNANDA Ru1z-LARREA 1l, RosA L6PEz2l, PILAR SANTAMARiA2>, MIRIAM SACRISTAN 1l, M. CARMEN Ru1z 1>, MYRIAM ZARAZAGA 1l, ANA RosA GuTIERREz 1l and CARM EN ToRREs 1l 1 ) Oepartment of Food and Agriculture, University of La Rioja, Logrof\o, Spain 2) Center of Agricultural Research ofLa Rioja (CIDA), Logrofio, Spain S u m m a r y : The protein fraction of white musts and wines obtained from grapes ( Vitis vinifera L. cv. Yiura) grown in northem Spain (Rioja) was investigated by SOS-PAGE. Under different fermentation conditions, e.g. aeration, must nitrogen content and yeast strain, SOS-PAGE pattems showed several bands with molecular weights ranging from 16 to 200 kOa. Higher weight proteins were glycosylated, whereas lower weight proteins were not. Under the experimental fermentation conditions none ofthe proteins showed any chemical modification that would alter the electrophoretical mobility or the covalent binding to their glycosylated moiety. K e y wo r d s : wine, soluble proteins, glycoproteins, free amino nitrogen content, grape juice. Introduction obtaining further knowledge ofprotein fraction ofwhite wine of cv. Viura. Different yeast strains were investigated and Solubleproteins in grape juice and wines mainly come different must nitrogen content and oxidising conditions from grapes; the protein content increases during grape during fermentation were used in order to establish possible maturation (ZoECKLEJN et al. 1995). It is weil established that modifications ofprotein content during alcoholic fermenta­ protein synthesis is activated after veraison and parallels tion.
    [Show full text]
  • To Foliar Nitrogen Fertilizati
    HORTSCIENCE 51(3):262–267. 2016. considering effect on grape amino acid content (Garde-Cerdan et al., 2014). Foliar N application can also be considered Response of ‘Italian Riesling’ Leaf as a tool for improvement of wine sensory characteristics since positive effect on grape Nitrogen Status and Fruit Composition volatile composition (Garde-Ceradan et al., 2014) and enhancement of grape and wine (Vitis vinifera L.) to Foliar Nitrogen phenolic content (Portu et al., 2015a, 2015b) have been determined. However, comparison of foliarly applied Fertilization different N forms did not include the study of Danijela Janjanin their effect on vine N supply, in addition to Institute of Agriculture and Tourism, Karla Huguesa 8, 52440 Porec, Croatia their effect on grape amino acid content. Also, as previously reported, foliar nitro- Marko Karoglan1 and Mirjana Herak Custi c gen application is usually performed around Faculty of Agriculture, University of Zagreb, Svetosimunska cesta 25, 10000 veraison (Cheng et Martinson, 2009; Jreij et al., 2009; Lacroux et al., 2008), which Zagreb, Croatia proved to be an effective way to improve Marijan Bubola berry YAN content since nitrogen transloca- tion in that period is directed mainly to Institute of Agriculture and Tourism, Karla Huguesa 8, 52440 Porec, Croatia berries. However, in vineyards with general Mirela Osrecak and Igor Palcic nitrogen deficiency caused by both soil and grapevine low N status that can occur as Faculty of Agriculture, University of Zagreb, Svetosimunska cesta 25, 10000 a problem since general improvement of Zagreb, Croatia nitrogen vine supply is required. As suggested by Neilsen et al. (2010) Additional index words.
    [Show full text]
  • Implications of Nitrogen Nutrition for Grapes, Fermentation and Wine
    242 Implications of nitrogen nutrition Australian Journal of Grape and Wine Research 11, 242–295, 2005 Implications of nitrogen nutrition for grapes, fermentation and wine SALLY-JEAN BELL and PAUL A. HENSCHKE The Australian Wine Research Institute, PO Box 197, Glen Osmond,Adelaide, SA 5064,Australia Corresponding authors: Sally-Jean Bell, facsimile: +61 8 8303 6601, email [email protected]; Paul A. Henschke, facsimile: +61 8 8303 6601, email [email protected] Abstract This review discusses the impacts of nitrogen addition in the vineyard and winery, and establishes the effects that nitrogen has on grape berry and wine composition and the sensory attributes of wine. Nitrogen is the most abundant soil-derived macronutrient in a grapevine, and plays a major role in many of the biological functions and processes of both grapevine and fermentative microorganisms. Manipulation of grapevine nitrogen nutrition has the potential to influence quality components in the grape and, ultimately, the wine. In addition, fermentation kinetics and formation of flavour-active metabolites are also affected by the nitrogen status of the must, which can be further manipulated by addition of nitrogen in the winery. The only consistent effect of nitrogen application in the vineyard on grape berry quality components is an increase in the concentration of the major nitrogenous compounds, such as total nitrogen, total amino acids, arginine, proline and ammonium, and consequently yeast- assimilable nitrogen (YAN). Both the form and amount of YAN have significant implications for wine quality. Low must YAN leads to low yeast populations and poor fermentation vigour, increased risk of sluggish/stuck/slow fermentations, increased production of undesirable thiols (e.g.
    [Show full text]
  • Technical Overview on Cider Production 23 23
    TALLINN, ESTONIA TALLINN, ESTONIA APRIL 25, 2017 APRIL 25, 2017 TECHNICAL OVERVIEW ON CIDER PRODUCTION TECHNICAL OVERVIEW TECHNICAL OVERVIEW ON CIDER PRODUCTION 23 23 LALLEMAND OENOLOGY LALLEMAND OENOLOGY TALLINN, ESTONIA, APRIL 25, 2017 TECHNICAL OVERVIEW ON CIDER PRODUCTION PROCEEDINGS OF THE XXVIIes ENTRETIENS SCIENTIFIQUES LALLEMAND ISBN 978-2-9815255-8-1 (printed version) ISBN 978-2-9815255-9-8 (pdf version) Legal deposit Bibliothèque et Archives nationales du Québec 2018 Library and Archives Canada 2018 DISCLAIMER: Lallemand has compiled the information contained herein and, to the best of its knowledge, the information is true and accurate. Lallemand offers this publication for use by winemaking professionals world- wide as a compendium of existing knowledge, both scientific and anecdotal. It is the user’s sole responsibility to determine whether any of the information contained herein is of benefit. The information, techniques and procedures presented in this publication are not to be considered as any type of expressed or implied guarantee for any aspect of the winemaking process in any wine-producing country. Lallemand Inc. Montréal, Canada H1W 2N8 The reprint or digital publication of any part of this book without permission from Lallemand is prohibited and illegal. CONTENTS CIDER CIDER: AN ANCIENT ART CIDERMAKING VS. WINEMAKING - IS THERE A IN A MODERN WORLD ............................................5 DIFFERENCE? ...........................................................19 Rebecca Mussell Amanda Stewart A TECHNICAL OVERVIEW OF FRENCH CIDER: LOCAL IMPORTANCE OF CIDER PRODUCTION IN FROM SPOILAGE CONTROL TO AROMATIC PROFILE ESTONIA (LOCAL PRODUCER SIIDRIKODA AS A PILOT CHARACTERIZATION ................................................9 PLANT FOR SCALE-UP YEAST APPLICATION) .........25 H. Guichard, P. Poupard, Jean-Michel Le Quéré and Rain Kuldjärv R.
    [Show full text]
  • Saccharomyces Cerevisiae in the Production of Fermented Beverages
    beverages Review Saccharomyces cerevisiae in the Production of Fermented Beverages Graeme M Walker 1,* and Graham G Stewart 2 1 Abertay University, Dundee, Scotland DD1 1HG, UK 2 Heriot-Watt University, Edinburgh, Scotland EH14 4AS, UK; [email protected] * Correspondence: [email protected]; Tel.: +44-1382-308658 Academic Editor: Edgar Chambers IV Received: 20 October 2016; Accepted: 11 November 2016; Published: 17 November 2016 Abstract: Alcoholic beverages are produced following the fermentation of sugars by yeasts, mainly (but not exclusively) strains of the species, Saccharomyces cerevisiae. The sugary starting materials may emanate from cereal starches (which require enzymatic pre-hydrolysis) in the case of beers and whiskies, sucrose-rich plants (molasses or sugar juice from sugarcane) in the case of rums, or from fruits (which do not require pre-hydrolysis) in the case of wines and brandies. In the presence of sugars, together with other essential nutrients such as amino acids, minerals and vitamins, S. cerevisiae will conduct fermentative metabolism to ethanol and carbon dioxide (as the primary fermentation metabolites) as the cells strive to make energy and regenerate the coenzyme NAD+ under anaerobic conditions. Yeasts will also produce numerous secondary metabolites which act as important beverage flavour congeners, including higher alcohols, esters, carbonyls and sulphur compounds. These are very important in dictating the final flavour and aroma characteristics of beverages such as beer and wine, but also in distilled beverages such as whisky, rum and brandy. Therefore, yeasts are of vital importance in providing the alcohol content and the sensory profiles of such beverages. This Introductory Chapter reviews, in general, the growth, physiology and metabolism of S.
    [Show full text]
  • Increasing Fermentation Reliability and Flavour Compound Formation by Wine Yeast
    Increasing fermentation reliability and flavour compound formation by wine yeast FINAL REPORT to GRAPE AND WINE RESEARCH & DEVELOPMENT CORPORATION Project Number: UA 00/5 Principal Investigator: Dr Vladimir Jiranek Research Organisation: University of Adelaide Date: February 2003 1 Final Report - February 2003 Project Title: Increasing fermentation reliability and flavour compound formation by wine yeast. Project File No: UA 00/5 Prepared by: Vladimir Jiranek and Jennie Gardner School of Agriculture and Wine, The University of Adelaide, PMB 1 Glen Osmond, SA 5064. Ph: (08) 8303 6651, email: [email protected] Abbreviations: CDGJM, chemically defined grape juice medium, FAN, free amino nitrogen, HNE, high nitrogen efficiency, PCR, polymerase chain reaction, ?, deletion, HPLC, high performance liquid chromatograph. Background The availability of a variety of wine yeast strains allows the winemaker to have greater control over the characteristics of oenological fermentation. Even so problem fermentations, specifically those which are sluggish or fail to complete are common. With the recognition of the central role of juice nitrogen deficiencies in many of these problem ferments, the use of nitrogen supplements has become widespread and routine. Nevertheless this strategy is not always successful. Nitrogen additions are a valuable tool for prevention and control of stuck fermentations, yet the timing of addition is critical (Bely et al., 1990) and the presence of residual nitrogen might favour microbial spoilage (Henschke and Jiranek, 1993) and formation of a carcinogen, ethyl carbamate (An and Ough, 1991). At any rate it is desirable to reduce additives made to premium wines. Another option for the prevention of stuck fermentation, which has yet to be employed is the use of “nitrogen efficient” wine yeast strains (Jiranek et al., 1991, 1995, Gardner et al., 2002).
    [Show full text]
  • Soil Nitrogen Fertilization Increases Yeast Assimilable Nitrogen
    HORTSCIENCE 55(8):1345–1355. 2020. https://doi.org/10.21273/HORTSCI15028-20 The rapid growth of the hard-cider (henceforth referred to as cider) industry within the United States during the past Soil Nitrogen Fertilization Increases decade offers an opportunity for apple [Malus ·domestica (Borkh.)] growers in the Yeast Assimilable Nitrogen United States to expand and diversify pro- duction, and potentially to increase their Concentrations in ‘Golden Russet’ and profitability with the cultivation of heirloom and high-tannin cultivars specifically for ci- der production (Peck and Knickerbocker, 2018; ‘Medaille d’Or’ Apples Used for Cider Peck et al., 2013). Many high-acid- and high- tannin-concentration cultivars sought after by Production cider producers for the sensory attributes of their juice are not available in sufficient Adam D. Karl and Michael G. Brown quantities to meet demand within the United School of Integrative Plant Science, Horticulture Section, Cornell University, States (Pashow, 2018). However, many of 121 Plant Science Building, Ithaca, NY 14853 these cultivars possess horticultural traits that pose challenges to their production in com- Sihui Ma, Ann Sandbrook, and Amanda C. Stewart mercial orchards, such as biennial bearing Department of Food Science and Technology, Virginia Polytechnic Institute and vigorous vegetative growth (Merwin and State University, HABB1 Room 401F, 1230 SW Washington Street, et al., 2008). Growers need information on Blacksburg, VA 24060 how horticultural management practices im- pact economic returns, fruit quality, and the Lailiang Cheng sensory attributes of the finished ciders to School of Integrative Plant Science, Horticulture Section, Cornell University, remain competitive and continue improving the quality of ciders available in the U.S.
    [Show full text]
  • Thesis Style Document
    Thesis Title: “Metabolism of nitrogenous wort components by brewers’ yeast” Christoforos Lekkas Submitted for the degree of Doctor of Philosophy Heriot-Watt University School of Life Sciences/ICBD June 2010 The copyright in this thesis is owned by the author. Any quotation from the thesis or use of any of the information contained in it must acknowledge this thesis as the source of the quotation or information. ABSTRACT A quantitative and qualitative identification of the nitrogenous constituents of unfermented and final fermented wort has been performed for five industrial yeast strains. Results highlight the importance of three key amino acids in yeast fermentation performance. Supplementation of wort using individual amino acids and ammonia clearly demonstrate lysine and arginine to be marker nitrogen wort constituents. Synergistic effects of individual wort free amino acids and ammonia on yeast fermentation efficiency were also examined. Further analysis of nitrogeneous wort constituents included examination of oligopeptides with a molecular weight of less than 500 Daltons and the influence of yeast proteases on the availability of small peptides during fermentation. Both lager and ale yeast can simultaneously use amino acids and small peptides as sources of assimilable nitrogen. Laboratory malt and mash analysis of 28 malt types revealed that 80-95% of total free amino nitrogen originates during malting and the remaining 5-20% during mashing. ACKNOWLEDGEMENT I would like to express my gratitude to all those who gave me the possibility to complete this thesis. I want to thank Scottish Courage Ltd for partially funding this thesis and especially my industrial supervisors Drs. Behnam Taidi and Jeff Hodgson for their scientific assistance on various project research related issues.
    [Show full text]
  • Impact of Protease Activity of Yeasts on Wine Fermentation and Formation of Volatile and Non-Volatile Metabolites
    Institute of Nutritional Science Justus Liebig-University Giessen, Germany and Section of Microbiology and Biochemistry Geisenheim Research Center, Germany Impact of protease activity of yeasts on wine fermentation and formation of volatile and non-volatile metabolites Thesis submitted in partial fulfilment of the requirements for the degree of Doctor oeconomiae trophologiaeque (Dr. oec. troph.) Submitted by Ni-orn Chomsri Lampang/Thailand 2008 This Ph.D. work was approved by the committee (Faculty 09: Agricultural and Nutritional Sciences, Home Economics and Environmental Management) of Justus Liebig-University Giessen, as a thesis to award the Doctor degree of oeconomiae trophologiaeque (Dr. oec. troph.) 1. Supervisor: Professor Dr. Hans Brueckner 2. Supervisor: Professor Dr. Doris Rauhut 3. Supervisor: Professor Dr. Sylvia Schnell Date of disputation: 10.07.2008 Contents 1 INTRODUCTION 1 1.1 Statement and significance of the study 1 1.2 Objectives 3 2 LITERATURE REVIEW 4 2.1 Yeasts 4 2.2 Proteolytic enzymes of yeasts 12 2.3 Nitrogen-containing compounds in winemaking 18 3 MATERIALS AND METHODS 33 4 RESULTS 44 4.1 Screening of wine yeasts that secrete extracellular proteases for the use in 44 grape juice fermentation 4.1.1 Protease production in synthetic medium 44 4.1.2 Protease production in grape juice 47 4.1.3 Effect of proteins isolated from must on yeast growth 48 4.2 Fermentative characteristics of non-Saccharomyces yeasts exhibiting 53 extracellular proteases in enological fermentation 4.3 Effect of yeast producing protease
    [Show full text]
  • Wine Proteins and Protein Stability
    Dr. Bruce Zoecklein WINE PROTEINS AND PROTEIN STABLITY DR. BRUCE ZOECKIEIN, PROFESSOR EMERITUS, VIRGINIA TECH Subject Outline Prefermentation Processing Considerations Fermentation and Post-Fermentation Processing Considerations Effect of Protein on Wine Stability Processing Considerations and Protein Stability Methods for Evaluation of Protein Stability Protein haze remains a significant problem for the wine industry and requires costly treatment with bentonite. Most of these costs are related to the loss of value of the wine recovered from the bentonite lees. The wine proteins responsible for haze are grape-related proteins. The must and subsequent wine protein content are affected by the following: variety vintage maturity condition of the fruit pH processing methodology Protein nitrogen content of wines varies between 10 and 275 mg/L. Despite the Dr. Bruce Zoecklein vast literature on protein instability, however, the actual protein levels at which wines will remain protein-stable are unknown (Waters et al., 2005). The mechanism of protein haze formation is not fully understood. Slow denaturation of wine proteins is thought to lead to protein aggregation, flocculation into a hazy suspension and, finally, formation of visual precipitates. The importance of non-proteinaceous factors, such as proanthocyanidins (Koch and Sajak, 1959; Yokotsuka et al., 1991), in white wine protein haze formation have been suspected. Other factors, such as polysaccharides, alcohol levels, and pH, have also been implicated (Mesquita et al., 2001; Siebert et al., 1996). Protein added to model wine does not precipitate or produce haze when heated, whereas visually obvious hazes occur when the same protein is added to a commercial wine. These observations led to the belief that precipitation involves two steps.
    [Show full text]
  • Nitrogen and Sulphur Foliar Fertilisation
    Nitrogen and Sulphur Foliar Fertilisation F. A. Bruwer, W. du Toit, A. Buica* Department of Viticulture and Oenology, P/Bag X1 Matieland, Stellenbosch 7600, South Africa Submitted for publication: October 2018 Accepted for publication: February 2019 Key words: Foliar fertilisation, nitrogen, sulphur, Chenin blanc, Sauvignon blanc, yeast assimilable nitrogen (YAN), glutathione, thiols The effects of fertilisation can reverberate from grapes through to wines. In wine, non-volatile compounds mainly influence the taste and flavour of wines, while volatile compounds play an important role in the aromatic expression. This review includes information on the presence of non-volatile and volatile compounds reportedly affected by nitrogen and sulphure foliar fertilisation, (bio)synthesis, and evolution throughout winemaking, their chemical properties, and their implications. The second part presents the status of the research on elucidating the influence and contribution of foliar fertilisation practices on the chemical compounds throughout winemaking, from the grape to the wine. INTRODUCTION A wine’s aroma, taste and flavour can contribute to the specific cultivar varieties. wine’s overall quality and determine if winemakers and The first part of this literature review will focus on consumers find it appealing (Marais, 1994). The aroma of various wine compounds and classes of compounds present a wine is the result of various interactions between different in grapes and wines that were shown to be affected by foliar chemical compounds found in the wine. These compounds fertilisation. The second part will present the status of the are generated at different stages and through various research on elucidating the influence and contribution of processes; some compounds originate from the grapes, while different fertilisation practices in relation to the chemical others are generated during fermentation or wine ageing.
    [Show full text]