TaqMan® Gene Signature Arrays

TaqMan® Human ABC Transporter Array

This array is part of a collection of TaqMan® Gene Signature References: Arrays that enable analysis of hundreds of TaqMan® Dean, M. 2002. The Human ATP-Binding Cassette (ABC) Gene Expression Assays on a micro fluidic card with Transporter Superfamily, http://www.ncbi.nlm.nih.gov/books/ minimal effort. bookres.fcgi/mono_001/mono_001.pdf

ABC (ATP-binding cassette) transporters are a superfamily of Dean, M. 2005. The genetics of ATP-binding cassette membrane proteins that translocate a variety of substrates transporters. Methods Enzymol. 400:409–429. across intra- and extra-cellular membranes, and are character- Glavinas, H., Krajcsi, P., Cserepes, J., and Sarkadi, B. 2004. The ized by homologous ATP-binding and large multispanning role of ABC transporters in drug resistance, metabolism and transmembrane domains. Substrates include drugs, lipids, toxicity. Curr Drug Deliv 1(1):27–42. metabolic products and sterols. Multidrug resistance in cancer cell lines and tumors is a result of overexpression of certain Taschenberger, G., Mougey, A., Shen, S., Lester, L.B., ABC transporters and a spectrum of human disorders having LaFranchi, S., and Shyng, S-L. 2002. Identification of a familial Mendelian and complex inheritance results directly or hyperinsulinism-causing mutation in the sulfonylurea receptor indirectly from mutations in ABC transporter genes. Diseases 1 that prevents normal trafficking and function of KATP for which ABC transporters are involved, or which are targeted channels. J Biol Chem 277:17139–17146. with drugs, include cystic fibrosis, type II diabetes, cholesterol UniProtKB/Swiss-Prot family/domain classification: ABC and bile transport defects, drug response phenotypes, anemia, transporter family, http://ca.expasy.org/cgi-bin/get-similar?name retinal degeneration, and neurological disease. =ABC%20transporter%20family There are three major groups of ABC transporters involved in cancer multidrug resistance (MDR). Additionally, ABC transporters play important roles in Absorption-Distribution- Metabolism-Excretion (ADME) and toxicology, which are also important in drug research and development.

The TaqMan Human ABC Transporter Array contains assays for 50 human genes in addition to 13 endogenous controls. The breakdown into the seven subfamilies is as follows:

Subfamily # of genes Alternative subfamily name

ABCA subfamily 13

ABCB subfamily 11 MDR subfamily

ABCC subfamily 13 Conjugate transporter subfamily

ABCD subfamily 4 ALD subfamily

ABCE subfamily 1

ABCF subfamily 3 EF3 subfamily

ABCG subfamily 5 White subfamily TaqMan ® Gene Signature Arrays

Gene Signature Array Name # of Ta rg e t s / C o n t ro l s Format Pack Size Part Number

Human ABC Transporter Array 50/14 Format 64 4 arrays/pack 4378700

Human ABC Transporter Array

A ABCF1 ABCF1 ABCF1 ABCD3 ABCD3 ABCD3 ABCD1 ABCD1 ABCD1 18S 18S 18S ABCC8 ABCC8 ABCC8 ABCC2 ABCC2 ABCC2 ABCB6 ABCB6 ABCB6 ABCA4 ABCA4 ABCA4 1 B ABCB1 ABCB1 ABCB1 ABCA3 ABCA3 ABCA3 ABCC6 ABCC6 ABCC6 ABCB11 ABCB11 ABCB11 ABCG2 ABCG2 ABCG2 ABCB8 ABCB8 ABCB8 ABCA7 ABCA7 ABCA7 ABCB7 ABCB7 ABCB7 C ABCD2 ABCD2 ABCD2 ABCA1 ABCA1 ABCA1 ABCC4 ABCC4 ABCC4 ABCF3 ABCF3 ABCF3 ABCC1 ABCC1 ABCC1 ABCG4 ABCG4 ABCG4 ABCG5 ABCG5 ABCG5 ABCG8 ABCG8 ABCG8 2 D ABCB4 ABCB4 ABCB4 TAP2 TAP2 TAP2 ABCA2 ABCA2 ABCA2 ABCG1 ABCG1 ABCG1 ABCD4 ABCD4 ABCD4 ABCC9 ABCC9 ABCC9 ABCC12 ABCC12 ABCC12 ABCA12 ABCA12 ABCA12 E ABCA9 ABCA9 ABCA9 CFTR CFTR CFTR ABCC3 ABCC3 ABCC3 ABCA5 ABCA5 ABCA5 ABCA6 ABCA6 ABCA6 ABCC10 ABCC10 ABCC10 ABCC13 ABCC13 ABCC13 ABCB10 ABCB10 ABCB10 3 F ABCA13 ABCA13 ABCA13 ABCF2 ABCF2 ABCF2 ABCB9 ABCB9 ABCB9 ABCB5 ABCB5 ABCB5 ABCA10 ABCA10 ABCA10 ABCA11 ABCA11 ABCA11 ABCA8 ABCA8 ABCA8 TAP1 TAP1 TAP1 G ABCC11 ABCC11 ABCC11 ABCC5 ABCC5 ABCC5 ABCE1 ABCE1 ABCE1 ACTB ACTB ACTB B2M B2M B2M GAPDH GAPDH GAPDH GUSB GUSB GUSB HMBS HMBS HMBS 4 H HPRT1 HPRT1 HPRT1 POLR2A POLR2A POLR2A PPIA PPIA PPIA RPLP0 RPLP0 RPLP0 TBP TBP TBP YWHAZ YWHAZ YWHAZ 18S 18S 18S PGK1 PGK1 PGK1 I ABCF1 ABCF1 ABCF1 ABCD3 ABCD3 ABCD3 ABCD1 ABCD1 ABCD1 18S 18S 18S ABCC8 ABCC8 ABCC8 ABCC2 ABCC2 ABCC2 ABCB6 ABCB6 ABCB6 ABCA4 ABCA4 ABCA4 5 J ABCB1 ABCB1 ABCB1 ABCA3 ABCA3 ABCA3 ABCC6 ABCC6 ABCC6 ABCB11 ABCB11 ABCB11 ABCG2 ABCG2 ABCG2 ABCB8 ABCB8 ABCB8 ABCA7 ABCA7 ABCA7 ABCB7 ABCB7 ABCB7 K ABCD2 ABCD2 ABCD2 ABCA1 ABCA1 ABCA1 ABCC4 ABCC4 ABCC4 ABCF3 ABCF3 ABCF3 ABCC1 ABCC1 ABCC1 ABCG4 ABCG4 ABCG4 ABCG5 ABCG5 ABCG5 ABCG8 ABCG8 ABCG8 6 L ABCB4 ABCB4 ABCB4 TAP2 TAP2 TAP2 ABCA2 ABCA2 ABCA2 ABCG1 ABCG1 ABCG1 ABCD4 ABCD4 ABCD4 ABCC9 ABCC9 ABCC9 ABCC12 ABCC12 ABCC12 ABCA12 ABCA12 ABCA12 M ABCA9 ABCA9 ABCA9 CFTR CFTR CFTR ABCC3 ABCC3 ABCC3 ABCA5 ABCA5 ABCA5 ABCA6 ABCA6 ABCA6 ABCC10 ABCC10 ABCC10 ABCC13 ABCC13 ABCC13 ABCB10 ABCB10 ABCB10 7 N ABCA13 ABCA13 ABCA13 ABCF2 ABCF2 ABCF2 ABCB9 ABCB9 ABCB9 ABCB5 ABCB5 ABCB5 ABCA10 ABCA10 ABCA10 ABCA11 ABCA11 ABCA11 ABCA8 ABCA8 ABCA8 TAP1 TAP1 TAP1 O ABCC11 ABCC11 ABCC11 ABCC5 ABCC5 ABCC5 ABCE1 ABCE1 ABCE1 ACTB ACTB ACTB B2M B2M B2M GAPDH GAPDH GAPDH GUSB GUSB GUSB HMBS HMBS HMBS 8 P HPRT1 HPRT1 HPRT1 POLR2A POLR2A POLR2A PPIA PPIA PPIA RPLP0 RPLP0 RPLP0 TBP TBP TBP YWHAZ YWHAZ YWHAZ 18S 18S 18S PGK1 PGK1 PGK1

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Port

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For Research Use Only. Not for use in diagnostic procedures. Practice of the patented 5’ Nuclease Process requires a license from Applied Biosystems. The purchase of TaqMan® Human ABC Transporter Array includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research when used with the separate purchase of an Authorized 5’ Nuc- lease Core Kit. No other patent rights are conveyed expressly, by implication, or by estoppel. For further information on purchasing licenses contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. The TaqMan® Array is covered by U.S. patents. Micro Fluidic Card developed in collaboration with 3M Company. © Copyright 2008, 2010. Applied Biosystems. All rights reserved. Applied Biosystems, and AB (Design) are registered trademarks of Applied Biosystems or its subsidiaries in the US and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Printed in the USA, 09/2010 Publication 127MI49-02

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