DOCSLIB.ORG

Taqman® Human ABC Transporter Array

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Taqman® Human ABC Transporter Array TaqMan® Gene Signature Arrays TaqMan® Human ABC Transporter Array This array is part of a collection of TaqMan® Gene Signature References: Arrays that enable analysis of hundreds of TaqMan® Dean, M. 2002. The Human ATP-Binding Cassette (ABC) Gene Expression Assays on a micro fluidic card with Transporter Superfamily, http://www.ncbi.nlm.nih.gov/books/ minimal effort. bookres.fcgi/mono_001/mono_001.pdf ABC (ATP-binding cassette) transporters are a superfamily of Dean, M. 2005. The genetics of ATP-binding cassette membrane proteins that translocate a variety of substrates transporters. Methods Enzymol. 400:409–429. across intra- and extra-cellular membranes, and are character- Glavinas, H., Krajcsi, P., Cserepes, J., and Sarkadi, B. 2004. The ized by homologous ATP-binding and large multispanning role of ABC transporters in drug resistance, metabolism and transmembrane domains. Substrates include drugs, lipids, toxicity. Curr Drug Deliv 1(1):27–42. metabolic products and sterols. Multidrug resistance in cancer cell lines and tumors is a result of overexpression of certain Taschenberger, G., Mougey, A., Shen, S., Lester, L.B., ABC transporters and a spectrum of human disorders having LaFranchi, S., and Shyng, S-L. 2002. Identification of a familial Mendelian and complex inheritance results directly or hyperinsulinism-causing mutation in the sulfonylurea receptor indirectly from mutations in ABC transporter genes. Diseases 1 that prevents normal trafficking and function of KATP for which ABC transporters are involved, or which are targeted channels. J Biol Chem 277:17139–17146. with drugs, include cystic fibrosis, type II diabetes, cholesterol UniProtKB/Swiss-Prot family/domain classification: ABC and bile transport defects, drug response phenotypes, anemia, transporter family, http://ca.expasy.org/cgi-bin/get-similar?name retinal degeneration, and neurological disease. =ABC%20transporter%20family There are three major groups of ABC transporters involved in cancer multidrug resistance (MDR). Additionally, ABC transporters play important roles in Absorption-Distribution- Metabolism-Excretion (ADME) and toxicology, which are also important in drug research and development. The TaqMan Human ABC Transporter Array contains assays for 50 human genes in addition to 13 endogenous controls. The breakdown into the seven subfamilies is as follows: Subfamily # of genes Alternative subfamily name ABCA subfamily 13 ABCB subfamily 11 MDR subfamily ABCC subfamily 13 Conjugate transporter subfamily ABCD subfamily 4 ALD subfamily ABCE subfamily 1 ABCF subfamily 3 EF3 subfamily ABCG subfamily 5 White subfamily TaqMan ® Gene Signature Arrays Gene Signature Array Name # of Ta rg e t s / C o n t ro l s Format Pack Size Part Number Human ABC Transporter Array 50/14 Format 64 4 arrays/pack 4378700 Human ABC Transporter Array A ABCF1 ABCF1 ABCF1 ABCD3 ABCD3 ABCD3 ABCD1 ABCD1 ABCD1 18S 18S 18S ABCC8 ABCC8 ABCC8 ABCC2 ABCC2 ABCC2 ABCB6 ABCB6 ABCB6 ABCA4 ABCA4 ABCA4 1 B ABCB1 ABCB1 ABCB1 ABCA3 ABCA3 ABCA3 ABCC6 ABCC6 ABCC6 ABCB11 ABCB11 ABCB11 ABCG2 ABCG2 ABCG2 ABCB8 ABCB8 ABCB8 ABCA7 ABCA7 ABCA7 ABCB7 ABCB7 ABCB7 C ABCD2 ABCD2 ABCD2 ABCA1 ABCA1 ABCA1 ABCC4 ABCC4 ABCC4 ABCF3 ABCF3 ABCF3 ABCC1 ABCC1 ABCC1 ABCG4 ABCG4 ABCG4 ABCG5 ABCG5 ABCG5 ABCG8 ABCG8 ABCG8 2 D ABCB4 ABCB4 ABCB4 TAP2 TAP2 TAP2 ABCA2 ABCA2 ABCA2 ABCG1 ABCG1 ABCG1 ABCD4 ABCD4 ABCD4 ABCC9 ABCC9 ABCC9 ABCC12 ABCC12 ABCC12 ABCA12 ABCA12 ABCA12 E ABCA9 ABCA9 ABCA9 CFTR CFTR CFTR ABCC3 ABCC3 ABCC3 ABCA5 ABCA5 ABCA5 ABCA6 ABCA6 ABCA6 ABCC10 ABCC10 ABCC10 ABCC13 ABCC13 ABCC13 ABCB10 ABCB10 ABCB10 3 F ABCA13 ABCA13 ABCA13 ABCF2 ABCF2 ABCF2 ABCB9 ABCB9 ABCB9 ABCB5 ABCB5 ABCB5 ABCA10 ABCA10 ABCA10 ABCA11 ABCA11 ABCA11 ABCA8 ABCA8 ABCA8 TAP1 TAP1 TAP1 G ABCC11 ABCC11 ABCC11 ABCC5 ABCC5 ABCC5 ABCE1 ABCE1 ABCE1 ACTB ACTB ACTB B2M B2M B2M GAPDH GAPDH GAPDH GUSB GUSB GUSB HMBS HMBS HMBS 4 H HPRT1 HPRT1 HPRT1 POLR2A POLR2A POLR2A PPIA PPIA PPIA RPLP0 RPLP0 RPLP0 TBP TBP TBP YWHAZ YWHAZ YWHAZ 18S 18S 18S PGK1 PGK1 PGK1 I ABCF1 ABCF1 ABCF1 ABCD3 ABCD3 ABCD3 ABCD1 ABCD1 ABCD1 18S 18S 18S ABCC8 ABCC8 ABCC8 ABCC2 ABCC2 ABCC2 ABCB6 ABCB6 ABCB6 ABCA4 ABCA4 ABCA4 5 J ABCB1 ABCB1 ABCB1 ABCA3 ABCA3 ABCA3 ABCC6 ABCC6 ABCC6 ABCB11 ABCB11 ABCB11 ABCG2 ABCG2 ABCG2 ABCB8 ABCB8 ABCB8 ABCA7 ABCA7 ABCA7 ABCB7 ABCB7 ABCB7 K ABCD2 ABCD2 ABCD2 ABCA1 ABCA1 ABCA1 ABCC4 ABCC4 ABCC4 ABCF3 ABCF3 ABCF3 ABCC1 ABCC1 ABCC1 ABCG4 ABCG4 ABCG4 ABCG5 ABCG5 ABCG5 ABCG8 ABCG8 ABCG8 6 L ABCB4 ABCB4 ABCB4 TAP2 TAP2 TAP2 ABCA2 ABCA2 ABCA2 ABCG1 ABCG1 ABCG1 ABCD4 ABCD4 ABCD4 ABCC9 ABCC9 ABCC9 ABCC12 ABCC12 ABCC12 ABCA12 ABCA12 ABCA12 M ABCA9 ABCA9 ABCA9 CFTR CFTR CFTR ABCC3 ABCC3 ABCC3 ABCA5 ABCA5 ABCA5 ABCA6 ABCA6 ABCA6 ABCC10 ABCC10 ABCC10 ABCC13 ABCC13 ABCC13 ABCB10 ABCB10 ABCB10 7 N ABCA13 ABCA13 ABCA13 ABCF2 ABCF2 ABCF2 ABCB9 ABCB9 ABCB9 ABCB5 ABCB5 ABCB5 ABCA10 ABCA10 ABCA10 ABCA11 ABCA11 ABCA11 ABCA8 ABCA8 ABCA8 TAP1 TAP1 TAP1 O ABCC11 ABCC11 ABCC11 ABCC5 ABCC5 ABCC5 ABCE1 ABCE1 ABCE1 ACTB ACTB ACTB B2M B2M B2M GAPDH GAPDH GAPDH GUSB GUSB GUSB HMBS HMBS HMBS 8 P HPRT1 HPRT1 HPRT1 POLR2A POLR2A POLR2A PPIA PPIA PPIA RPLP0 RPLP0 RPLP0 TBP TBP TBP YWHAZ YWHAZ YWHAZ 18S 18S 18S PGK1 PGK1 PGK1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Port More arrays will be available soon! Register to receive new Gene Signature Array product announcements, or suggest an array at taqmanarray.appliedbiosystems.com For Research Use Only. Not for use in diagnostic procedures. Practice of the patented 5’ Nuclease Process requires a license from Applied Biosystems. The purchase of TaqMan® Human ABC Transporter Array includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research when used with the separate purchase of an Authorized 5’ Nuc- lease Core Kit. No other patent rights are conveyed expressly, by implication, or by estoppel. For further information on purchasing licenses contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. The TaqMan® Array is covered by U.S. patents. Micro Fluidic Card developed in collaboration with 3M Company. © Copyright 2008, 2010. Applied Biosystems. All rights reserved. Applied Biosystems, and AB (Design) are registered trademarks of Applied Biosystems or its subsidiaries in the US and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Printed in the USA, 09/2010 Publication 127MI49-02 Headquarters International Sales 850 Lincoln Centre Drive | Foster City, CA 94404 USA For our office locations please call the division Phone 650.638.5800 | Toll Free 800.345.5224 headquarters or refer to our Web site at www.appliedbiosystems.com www.appliedbiosystems.com/about/offices.cfm.
Load more

Recommended publications
  • Targeted Sequence Capture and High-Throughput Sequencing in the Molecular Diagnosis of Ichthyosis and Other Skin Diseases
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE providedCA by Elsevier Scott - Publisheret al. Connector Sequence Capture in Molecular Diagnosis of Skin Diseases Immunohistochemical staining indi- 1The Wellcome Trust Center for Human cause of Olmsted syndrome. Am J Hum cated that MBTPS2 is mainly expressed Genetics, Nuffield Department of Clinical Genet 90:558–64 Medicine, University of Oxford, Oxford, UK; Mevorah B, Goldberg I, Sprecher E et al. (2005) in the upper granular layer in normal 2 Centre for Cutaneous Research, The Blizard Olmsted syndrome: mutilating palmo- skin, as previously shown (Aten et al., Institute, Barts & The London School of plantar keratoderma with periorificial 2010); however, in OS skin, MBTPS2 Medicine and Dentistry, Queen Mary keratotic plaques. J Am Acad Dermatol 53: University of London, London, UK; S266–72 was expressed throughout the epi- 3 dermis (Figure 2c). There was no Department of Dermatology, Jundishapur Naiki M, Mizuno S, Yamada K et al. (2012) University of Medical Sciences, Ahvaz, Iran; MBTPS2 mutation causes BRESEK/BRESHECK 4 apparent difference in MBTPS2 locali- Genetic Department, Kerman University of syndrome. Am J Med Genet A 158A:97–102 zation in the skin of a KFSD patient with 5 Medical Sciences, Kerman, Iran and Darwin Oeffner F, Fischer G, Happle R et al. (2009) IFAP the p.N508S mutation (Aten et al., Building, University College London Genetics syndrome is caused by deficiency in MBTPS2, 2010). It is unclear why this is but it Institute, University College London, London, an intramembrane zinc metalloprotease essen- may be because of differences in UK tial for cholesterol homeostasis and ER stress E-mail: [email protected] response.
    [Show full text]
  • The Translational Expression of ABCA2 and ABCA3 Is a Strong Prognostic Biomarker for Multidrug Resistance in Pediatric Acute Lymphoblastic Leukemia
    Journal name: OncoTargets and Therapy Article Designation: Original Research Year: 2017 Volume: 10 OncoTargets and Therapy Dovepress Running head verso: Aberuyi et al Running head recto: ABCA2/A3 transporters and multidrug-resistant ALL open access to scientific and medical research DOI: http://dx.doi.org/10.2147/OTT.S140488 Open Access Full Text Article ORIGINAL RESEARCH The translational expression of ABCA2 and ABCA3 is a strong prognostic biomarker for multidrug resistance in pediatric acute lymphoblastic leukemia Narges Aberuyi1 Purpose: The aim of this work was to study the correlation between the expressions of the Soheila Rahgozar1 ABCA2 and ABCA3 genes at the mRNA and protein levels in children with acute lymphoblastic Zohreh Khosravi Dehaghi1 leukemia (ALL) and the effects of this association on multidrug resistance (MDR). Alireza Moafi2 Materials and methods: Sixty-nine children with de novo ALL and 25 controls were Andrea Masotti3,* enrolled in the study. Mononuclear cells were isolated from the bone marrow. The mRNA Alessandro Paolini3,* levels of ABCA2 and ABCA3 were measured by real-time polymerase chain reaction (PCR). Samples with high mRNA levels were assessed for respective protein levels by Western blot- 1 Department of Biology, Faculty ting. Following the first year of treatment, persistent monoclonality of T-cell gamma receptors of Science, University of Isfahan, 2Department of Pediatric- or immunoglobulin H (IgH) gene rearrangement was assessed and considered as the MDR. Hematology-Oncology, Sayed-ol- The tertiary structure of ABCA2 was predicted using Phyre2 and I-TASSER web systems Shohada Hospital, Isfahan University and compared to that of ABCA3, which has been previously reported.
    [Show full text]
  • EXTENDED CARRIER SCREENING Peace of Mind for Planned Pregnancies
    Focusing on Personalised Medicine EXTENDED CARRIER SCREENING Peace of Mind for Planned Pregnancies Extended carrier screening is an important tool for prospective parents to help them determine their risk of having a child affected with a heritable disease. In many cases, parents aren’t aware they are carriers and have no family history due to the rarity of some diseases in the general population. What is covered by the screening? Genomics For Life offers a comprehensive Extended Carrier Screening test, providing prospective parents with the information they require when planning their pregnancy. Extended Carrier Screening has been shown to detect carriers who would not have been considered candidates for traditional risk- based screening. With a simple mouth swab collection, we are able to test for over 419 genes associated with inherited diseases, including Fragile X Syndrome, Cystic Fibrosis and Spinal Muscular Atrophy. The assay has been developed in conjunction with clinical molecular geneticists, and includes genes listed in the NIH Genetic Test Registry. For a list of genes and disorders covered, please see the reverse of this brochure. If your gene of interest is not covered on our Extended Carrier Screening panel, please contact our friendly team to assist you in finding a gene test panel that suits your needs. Why have Extended Carrier Screening? Extended Carrier Screening prior to pregnancy enables couples to learn about their reproductive risk and consider a complete range of reproductive options, including whether or not to become pregnant, whether to use advanced reproductive technologies, such as preimplantation genetic diagnosis, or to use donor gametes.
    [Show full text]
  • ABCG1 (ABC8), the Human Homolog of the Drosophila White Gene, Is a Regulator of Macrophage Cholesterol and Phospholipid Transport
    ABCG1 (ABC8), the human homolog of the Drosophila white gene, is a regulator of macrophage cholesterol and phospholipid transport Jochen Klucken*, Christa Bu¨ chler*, Evelyn Orso´ *, Wolfgang E. Kaminski*, Mustafa Porsch-Ozcu¨ ¨ ru¨ mez*, Gerhard Liebisch*, Michael Kapinsky*, Wendy Diederich*, Wolfgang Drobnik*, Michael Dean†, Rando Allikmets‡, and Gerd Schmitz*§ *Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93042 Regensburg, Germany; †National Cancer Institute, Laboratory of Genomic Diversity, Frederick, MD 21702-1201; and ‡Departments of Ophthalmology and Pathology, Columbia University, Eye Research Addition, New York, NY 10032 Edited by Jan L. Breslow, The Rockefeller University, New York, NY, and approved November 3, 1999 (received for review June 14, 1999) Excessive uptake of atherogenic lipoproteins such as modified low- lesterol transport. Although several effector molecules have been density lipoprotein complexes by vascular macrophages leads to proposed to participate in macrophage cholesterol efflux (6, 9), foam cell formation, a critical step in atherogenesis. Cholesterol efflux including endogenous apolipoprotein E (10) and the cholesteryl mediated by high-density lipoproteins (HDL) constitutes a protective ester transfer protein (11), the detailed molecular mechanisms mechanism against macrophage lipid overloading. The molecular underlying cholesterol export in these cells have not yet been mechanisms underlying this reverse cholesterol transport process are characterized. currently not fully understood. To identify effector proteins that are Recently, mutations of the ATP-binding cassette (ABC) trans- involved in macrophage lipid uptake and release, we searched for porter ABCA1 gene have been causatively linked to familial HDL genes that are regulated during lipid influx and efflux in human deficiency and Tangier disease (12–14).
    [Show full text]
  • Molecular Characterization and Structural Implications of 25 New ABCB4 Mutations in Progressive Familial Intrahepatic Cholestasis Type 3 (PFIC3)
    European Journal of Human Genetics (2007) 15, 1230–1238 & 2007 Nature Publishing Group All rights reserved 1018-4813/07 $30.00 www.nature.com/ejhg ARTICLE Molecular characterization and structural implications of 25 new ABCB4 mutations in progressive familial intrahepatic cholestasis type 3 (PFIC3) Dario Degiorgio1, Carla Colombo2, Manuela Seia1, Luigi Porcaro1, Lucy Costantino1, Laura Zazzeron2, Domenico Bordo3 and Domenico A Coviello*,1 1Laboratorio di Genetica Medica, Fondazione IRCCS, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milano, Italy; 2Centro Fibrosi Cistica, Universita` degli Studi di Milano, Fondazione IRCCS, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milano, Italy; 3Bioinformatica e Proteomica Strutturale, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy Progressive familial intrahepatic cholestasis type 3 (PFIC3) is an autosomal-recessive disorder due to mutations in the ATP-binding cassette, subfamily B, member 4 gene (ABCB4). ABCB4 is the liver-specific membrane transporter of phosphatidylcholine, a major and exclusive component of mammalian bile. The disease is characterized by early onset of cholestasis with high serum c-glutamyltranspeptidase activity, which progresses into cirrhosis and liver failure before adulthood. Presently, about 20 distinct ABCB4 mutations associated to PFIC3 have been described. We report the molecular characterization of 68 PFIC3 index cases enrolled in a multicenter study, which represents the largest cohort of PFIC3 patients screened for ABCB4 mutations to date. We observed 31 mutated ABCB4 alleles in 18 index cases with 29 distinct mutations, 25 of which are novel. Despite the lack of structural information on the ABCB4 protein, the elucidation of the three-dimensional structure of bacterial homolog allows the three-dimensional model of ABCB4 to be built by homology modeling and the position of the mutated amino-acids in the protein tertiary structure to be located.
    [Show full text]
  • ABCB7 Gene ATP Binding Cassette Subfamily B Member 7
    ABCB7 gene ATP binding cassette subfamily B member 7 Normal Function The ABCB7 gene provides instructions for making a protein known as an ATP-binding cassette (ABC) transporter. ABC transporter proteins carry many types of molecules across membranes in cells. The ABCB7 protein is located in the inner membrane of cell structures called mitochondria. Mitochondria are involved in a wide variety of cellular activities, including energy production, chemical signaling, and regulation of cell growth and division. In the mitochondria of developing red blood cells (erythroblasts), the ABCB7 protein plays a critical role in the production of heme. Heme contains iron and is a component of hemoglobin, the protein that carries oxygen in the blood. The ABCB7 protein is also involved in the formation of certain proteins containing clusters of iron and sulfur atoms (Fe-S clusters). Researchers suspect that the ABCB7 protein transports Fe-S clusters from mitochondria, where they are formed, to the surrounding cellular fluid (cytosol), where they can be incorporated into proteins. Overall, researchers believe that the ABCB7 protein helps maintain an appropriate balance of iron (iron homeostasis) in developing red blood cells. Health Conditions Related to Genetic Changes X-linked sideroblastic anemia and ataxia At least three mutations in the ABCB7 gene have been identified in people with X-linked sideroblastic anemia with ataxia. Each of these mutations changes a single protein building block (amino acid) in the ABCB7 protein, slightly altering its structure. These changes disrupt the protein's usual role in heme production and iron homeostasis. Anemia results when heme cannot be produced normally, and therefore not enough hemoglobin is made.
    [Show full text]
  • Structures and Functions of Mitochondrial ABC Transporters
    ATP-binding cassette transporters: from mechanism to organism 943 Structures and functions of mitochondrial ABC transporters Theresia A. Schaedler*, Belinda Faust†, Chitra A. Shintre†, Elisabeth P. Carpenter†, Vasundara Srinivasan‡, Hendrik W. van Veen§ and Janneke Balk1 *Department of Biological Chemistry and Crop Protection, Rothamsted Research, West Common, Harpenden, AL5 2JQ, U.K. †Structural Genomics Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, OX3 7DQ, U.K. ‡LOEWE center for synthetic microbiology (SYNMIKRO) and Philipps University, D-35043 Marburg, Germany §Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1PD, U.K. John Innes Centre and University of East Anglia, Colney Lane, Norwich, NR4 7UH, U.K. Abstract A small number of physiologically important ATP-binding cassette (ABC) transporters are found in mitochondria. Most are half transporters of the B group forming homodimers and their topology suggests they function as exporters. The results of mutant studies point towards involvement in iron cofactor biosynthesis. In particular, ABC subfamily B member 7 (ABCB7) and its homologues in yeast and plants are required for iron-sulfur (Fe-S) cluster biosynthesis outside of the mitochondria, whereas ABCB10 is involved in haem biosynthesis. They also play a role in preventing oxidative stress. Mutations in ABCB6 and ABCB7 have been linked to human disease. Recent crystal structures of yeast Atm1 and human ABCB10 have been key to identifying substrate-binding sites and transport mechanisms. Combined with in vitro and in vivo studies, progress is being made to find the physiological substrates of the different mitochondrial ABC transporters. Sequence analysis of mitochondrial ABC The ABCB7 group, which includes the ABC transporters transporters of the mitochondria Atm1 in yeast and ATM3 in Arabidopsis, Mitochondria of most eukaryote species harbour 2–4 can be found in virtually all eukaryotic species.
    [Show full text]
  • Datasheet: MCA2682A647 Product Details
    Datasheet: MCA2682A647 Description: RAT ANTI MOUSE ABCA2:Alexa Fluor® 647 Specificity: ABCA2 Other names: ATP BINDING CASSETTE 2 Format: ALEXA FLUOR® 647 Product Type: Monoclonal Antibody Clone: 9A2-51.3 Isotype: IgG2a Quantity: 100 TESTS/1ml Product Details Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Flow Cytometry (1) Neat - 1/10 Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. (1)Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose. Target Species Mouse Product Form Purified IgG conjugated to Alexa Fluor® 647- liquid Max Ex/Em Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 Preparation Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Buffer Solution Phosphate buffered saline Preservative 0.09% Sodium Azide (NaN3) Stabilisers 1% Bovine Serum Albumin Approx. Protein IgG concentration 0.05mg/ml Concentrations Immunogen ABCA2 transfected HeLa cells. External Database UniProt: Links Page 1 of 3 P41234 Related reagents Entrez Gene: 11305 Abca2 Related reagents Synonyms Abc2 Specificity Rat anti Mouse ABCA2 antibody, clone 9A2-51.3 recognizes murine adenosine triphosphate (ATP) binding cassette transporter 2 (ABCA2).
    [Show full text]
  • 5' Untranslated Region Elements Show High Abundance and Great
    International Journal of Molecular Sciences Article 0 5 Untranslated Region Elements Show High Abundance and Great Variability in Homologous ABCA Subfamily Genes Pavel Dvorak 1,2,* , Viktor Hlavac 2,3 and Pavel Soucek 2,3 1 Department of Biology, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic 2 Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic; [email protected] (V.H.); [email protected] (P.S.) 3 Toxicogenomics Unit, National Institute of Public Health, 100 42 Prague, Czech Republic * Correspondence: [email protected]; Tel.: +420-377593263 Received: 7 October 2020; Accepted: 20 November 2020; Published: 23 November 2020 Abstract: The 12 members of the ABCA subfamily in humans are known for their ability to transport cholesterol and its derivatives, vitamins, and xenobiotics across biomembranes. Several ABCA genes are causatively linked to inborn diseases, and the role in cancer progression and metastasis is studied intensively. The regulation of translation initiation is implicated as the major mechanism in the processes of post-transcriptional modifications determining final protein levels. In the current bioinformatics study, we mapped the features of the 50 untranslated regions (50UTR) known to have the potential to regulate translation, such as the length of 50UTRs, upstream ATG codons, upstream open-reading frames, introns, RNA G-quadruplex-forming sequences, stem loops, and Kozak consensus motifs, in the DNA sequences of all members of the subfamily. Subsequently, the conservation of the features, correlations among them, ribosome profiling data as well as protein levels in normal human tissues were examined. The 50UTRs of ABCA genes contain above-average numbers of upstream ATGs, open-reading frames and introns, as well as conserved ones, and these elements probably play important biological roles in this subfamily, unlike RG4s.
    [Show full text]
  • Molecular Structure of a Novel Cholesterol-Responsive a Subclass ABC Transporter, ABCA9
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by University of Regensburg Publication Server BBRC Biochemical and Biophysical Research Communications 295 (2002) 408–416 www.academicpress.com Molecular structure of a novel cholesterol-responsive A subclass ABC transporter, ABCA9 Armin Piehler,1 Wolfgang E. Kaminski,1 Juurgen€ J. Wenzel, Thomas Langmann, and Gerd Schmitz* Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauß-Allee 11, D-93042, Regensburg, Germany Received 25 May 2002 Abstract We recently identified a novel ABC A subclass transporter, ABCA6, in human macrophages. Here, we report the molecular cloning of an additional ABC A subfamily transporter from macrophages denoted ABCA9. The identified coding sequence is 4.9 kb in size and codes for a 1624 amino acid protein product. In accordance with the proposed nomenclature, the novel transporter was designated ABCA9. The putative full-length ABC transporter polypeptide consists of two transmembrane domains and two nu- cleotide binding folds and thus conforms to the group of full-size ABC transporters. We identified alternative ABCA9 mRNA variants in human macrophages that predict the existence of three truncated forms of the novel transporter. Among the human ABC A subfamily transporters, ABCA9 exhibits the highest amino acid sequence homology with ABCA8 (72%) and ABCA6 (60%), respectively. The striking amino acid sequence similarity between these transporter molecules supports the notion that they represent an evolutionary more recently emerged subgroup within the family of ABC A transporters, which we refer to as ‘‘ABCA6-like transporters.’’ ABCA9 mRNA is ubiquitously expressed with the highest mRNA levels in heart, brain, and fetal tissues.
    [Show full text]
  • ABCB6 Is a Porphyrin Transporter with a Novel Trafficking Signal That Is Conserved in Other ABC Transporters Yu Fukuda University of Tennessee Health Science Center
    University of Tennessee Health Science Center UTHSC Digital Commons Theses and Dissertations (ETD) College of Graduate Health Sciences 12-2008 ABCB6 Is a Porphyrin Transporter with a Novel Trafficking Signal That Is Conserved in Other ABC Transporters Yu Fukuda University of Tennessee Health Science Center Follow this and additional works at: https://dc.uthsc.edu/dissertations Part of the Chemicals and Drugs Commons, and the Medical Sciences Commons Recommended Citation Fukuda, Yu , "ABCB6 Is a Porphyrin Transporter with a Novel Trafficking Signal That Is Conserved in Other ABC Transporters" (2008). Theses and Dissertations (ETD). Paper 345. http://dx.doi.org/10.21007/etd.cghs.2008.0100. This Dissertation is brought to you for free and open access by the College of Graduate Health Sciences at UTHSC Digital Commons. It has been accepted for inclusion in Theses and Dissertations (ETD) by an authorized administrator of UTHSC Digital Commons. For more information, please contact [email protected]. ABCB6 Is a Porphyrin Transporter with a Novel Trafficking Signal That Is Conserved in Other ABC Transporters Document Type Dissertation Degree Name Doctor of Philosophy (PhD) Program Interdisciplinary Program Research Advisor John D. Schuetz, Ph.D. Committee Linda Hendershot, Ph.D. James I. Morgan, Ph.D. Anjaparavanda P. Naren, Ph.D. Jie Zheng, Ph.D. DOI 10.21007/etd.cghs.2008.0100 This dissertation is available at UTHSC Digital Commons: https://dc.uthsc.edu/dissertations/345 ABCB6 IS A PORPHYRIN TRANSPORTER WITH A NOVEL TRAFFICKING SIGNAL THAT
    [Show full text]
  • ABCA12 (N-16): Sc-48435
    SAN TA C RUZ BI OTEC HNOL OG Y, INC . ABCA12 (N-16): sc-48435 BACKGROUND CHROMOSOMAL LOCATION The ATP binding cassette (ABC) transporters, or traffic ATPases, constitute Genetic locus: ABCA12 (human) mapping to 2q35; Abca12 (mouse) mapping an expansive family of proteins accountable for the transport of a wide to 1 C3. variety of substrates across cell membranes in both prokaryotic and eukary - otic cells. They also aid in the regulation of lipid transport and membrane SOURCE trafficking. ABCA12 (ATP-binding cassette, subfamily A, member 12) contains ABCA12 (N-16) is an affinity purified goat polyclonal antibody raised against two transmembrane (TM) domains, each with six membrane-spanning seg - a peptide mapping within an internal region of ABCA12 of human origin. ments and two nucleotide-binding domains (NBDs), which are located in the cytoplasm. ABCA12 is expressed in normal human keratinocytes (RT-PCR PRODUCT reveals expression in placenta, testis, fetal brain and skin) and is upregulated during keratinization. Immunoelectron microscopy reveals that the ABCA12 Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide protein is located in lamellar granules in the upper epidermal keratinocytes and 0.1% gelatin. of human skin. The ABCA12 gene, which synthesizes a 2,595 amino acid Blocking peptide available for competition studies, sc-48435 P, (100 µg protein, may produce an alternative splice variant with an in-frame deletion peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). leading to truncation of 79 amino acids. APPLICATIONS REFERENCES ABCA12 (N-16) is recommended for detection of ABCA12 of mouse, rat and 1.
    [Show full text]