Ethoxyquin: an Antioxidant Used in Animal Feed

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Hindawi Publishing Corporation International Journal of Food Science Volume 2013, Article ID 585931, 12 pages http://dx.doi.org/10.1155/2013/585931 Review Article Ethoxyquin: An Antioxidant Used in Animal Feed Alina BBaszczyk,1 Aleksandra Augustyniak,1 and Janusz Skolimowski2 1 Department of General Genetics, Molecular Biology and Plant Biotechnology, Faculty of Biology and Environmental Protection, University of Łod´ z,Banacha12/16,90-237Ł´ od´ z,´ Poland 2 Department of Organic Chemistry, Faculty of Chemistry, University of Łod´ z,´ Tamka 12, 91-403 Łod´ z,´ Poland Correspondence should be addressed to Alina Błaszczyk; [email protected] Received 15 January 2013; Accepted 2 April 2013 Academic Editor: Angel´ Medina-Vaya´ Copyright © 2013 Alina Błaszczyk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Ethoxyquin (EQ, 6-ethoxy-1,2-dihydro-2,2,4-trimethylquinoline) is widely used in animal feed in order to protect it against lipid peroxidation. EQ cannot be used in any food for human consumption (except spices, e.g., chili), but it can pass from feed to farmed fish, poultry, and eggs, so human beings can be exposed to this antioxidant. The manufacturer Monsanto Company (USA) performed a series of tests on ethoxyquin which showed its safety. Nevertheless, some harmful effects in animals and people occupationally exposed to it were observed in 1980’s which resulted in the new studies undertaken to reevaluate its toxicity. Here, we present the characteristics of the compound and results of the research, concerning, for example, products of its metabolism and oxidation or searching for new antioxidants on the EQ backbone. 1. Introduction pharmaceuticals and cosmetics. There are many controversies abouttheuseofthesetwoantioxidantsinfoods.Some During storage of animal feed many different processes may experimental studies have reported that both BHT and occur which alter their initial natural proprieties. First of all, BHA have tumour-promoting activity [1, 2]. On the other lipids undergo peroxidation, the process during which they hand, there were reports on anticarcinogenic properties of are deteriorated in a free radical autocatalytic oxidation chain these antioxidants when they are used at low concentrations reaction with atmospheric oxygen. Lipid autooxidation is a [3]. Human exposures are at least 1000-fold below those cascade phenomenon ensuring continuous delivery of free associated with any neoplastic actions in laboratory animals radicals, which initiate continuous peroxidation. This process thusitisassumedthattheyarenotharmfulforhumanbeings results in food rancidity which manifests itself as the change [3, 4]. of taste, scent, and color as well as decrease in shelf life of the The third compound, EQ, is one of the best known feed product. Natural or synthetic antioxidants are usually used to antioxidants for domestic animal and fish. Its unquestionable slow down or stop lipid peroxidation and in consequence to advantage is its high antioxidant capacity and low production preserve freshness of the product. Many natural antioxidants, costs. However, some of the authors have suggested that it such as tocopherols, vitamin C, flavonoids, for a short is responsible for a wide range of health-related problems period, may be effective in food preserving, but in many in dogs as well as in humans [5–9]. Due to the increased cases such protection is not sufficient. Therefore synthetic use of this antioxidant it was nominated by FDA (US Food antioxidants are widely used, among which BHT (butylated and Drug Administration) for carcinogenicity testing [10]. hydroxytoluene), BHA (butylated hydroxyanisole), and EQ ThetestswerecarriedoutbyMonsantoCompany(USA),EQ (ethoxyquin) are the most frequent. However, some effects of producer, and after that in 1977 FDA requested for optional synthetic antioxidants are not always beneficial for our health. lowering of the maximum level of EQ in complete dog foods Antioxidants such as BHA or BHT have been widely used from allowed 150 ppm (0.015%) to 75 ppm (0.0075%). At the for many years to preserve freshness, flavor, and colour of same time new studies were started by the Pet Food Institute foodsandanimalfeedsaswellastoimprovethestabilityof to determine whether even lower EQ levels (between 30 and 2 International Journal of Food Science 60 ppm) would provide antioxidant protection for dog food Table 1: Permitted amounts of EQ in different products approved ∗ [11]. by FDA . Ethoxyquin is also known as Santoquin, Santoflex, Product Dose [ppm] Quinol. It was originally developed in rubber industry to prevent rubber from cracking due to oxidation of isoprene Animal feed 150 [12]. The Monsanto Company (USA) taking into account its Spices 150 high antioxidant efficiency and stability as well as low costs Uncooked fat of meat from animals (except poultry) 5 of synthesis refined it later for use as a preservative in animal Uncookedliverandfatofpoultry 3 feeds because it protects against lipid peroxidation and Uncooked muscle meat of animals 0.5 stabilizes fat soluble vitamins (A, E). Presently, ethoxyquin is Poultry eggs 0.5 used primarily as an antioxidant in canned pet food and in Milk 0 feed intended for farmed fish or poultry. Pear 3 Theuseofethoxyquinisnotpermittedinfoodsintended ∗ According to the Code of Federal Regulations (CFR), Title 21, Parts 573.380, for human, except preserving powdered paprika and chili 573.400, 172.140. colour and using it as an antiscald agent in pears and apples (inhibition of “brown spots” development). However, because EQ is used as a feed antioxidant it can be also found in other products intended for human consumption like fish meal, fish The other metabolic pathways include hydroxylation and oils, and other oils, fats, and meat (Table 1). An acceptable glucuronidation at position 8-C, deethylation at 6-C and −1 daily intake (ADI) of EQ for human (0–0.005 mg kg bw) epoxidation between positions 3-C and 4-C [21]. The main based on the results obtained from studies on dogs was metabolites observed may be different depending on ani- established in 1998 [13, 14]. mal species. In mice mainly glucuronide metabolites were This paper presents characteristics of ethoxyquin with detected while in rats those result from conjugation of EQ regard to its properties, metabolism, toxicity, possible car- with sulfate. cinogenicity, and antioxidant activity. In the studies of Bohne et al. [23, 26] parent EQ, dem- ethylated EQ (DEQ), quinone imine (QI), and EQ dimer (EQDM) were observed in salmonid fish after long-term 2. Physical and Chemical Properties of dietary exposure to EQ. It was in agreement with the results Ethoxyquin (EQ) obtained earlier by Skaare and Roald [27]. EQ is considered asamodelinducerofphaseIIenzymesinvolvedinthe For the first time EQ was synthesized in 1921 by Knoevenagel metabolism of xenobiotics, but influence of EQ on phase I [15]. The synthesis was based on condensation of aniline with enzyme gene transcript levels was also observed [28, 29]. The molecules of acetone or its analogues [15, 16]. Błaszczyk et keyroleinmediatingphaseIreactions(e.g.,oxidationor al. [16] synthesized EQ from p-phenetidine (4-ethoxyaniline) reduction) producing more hydrophilic compounds is played and diacetone alcohol in the presence of p-toluenesulfonic by the CYP (cytochrome P450) enzyme family. Bohne et al. acid or iodine. Pure ethoxyquin (EQ; 6-ethoxy-1,2-dihydro- [28, 29] observed the alteration of CYP3A gene expression; 2,2,4-trimethylquinoline; CAS number 91-53-2; Figure 1)is an increase in the amount of CYP3A transcripts was detected a light yellow liquid, but it changes color to brown if it in salmon after feeding them with the diet containing EQ at −1 is exposed to oxygen [12]. It also tends to polymerize on the highest dose used (1800 mg kg ). The authors speculate exposure to light and air. The scent of EQ is described as that EQ may regulate CYP3 gene expression by interaction, mercaptan like. As a nonpolar substance EQ is soluble only for example, with pregnane X nuclear receptor (PXR) whose in organic solvents. Some of the additional properties of EQ function is to sense the presence of toxic xenobiotics and are presented in Table 2. in response enhance the expression of proteins involved in their detoxification. On the other hand, CYP1A1 gene 3. Biotransformation of EQ expression, which was described as an exposure biomarker to both endogenous and exogenous compounds [30], was 3.1. Animals. Ethoxyquin is rapidly absorbed from gastroin- not increased after dietary exposure of salmonid fish toEQ testinal tract of laboratory animals like rats and mice. Peak and during the depuration period a trend toward down- blood concentration of the compound is observed within regulation was noted [28, 29]. Such an effect was observed 1h.DistributionofEQinanimalbodyissimilarwhenit despite the increase in the expression of AhR mRNA (AhR, is administered orally and intravenously. Small amounts of cytosolic transcription factor responsible for changes in gene parent EQ were detected in liver, kidney, and adipose tissue transcription). This effect can be explained in several ways. and fish muscles [20–24]. It is excreted predominantly as For example, the parent EQ may bind CYP1A1 protein metabolites via urine. Metabolism of EQ was studied in rats, and as a result may inhibit the gene expression and activ-
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