DOCSLIB.ORG

Nocardiopsis Proteases

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Nocardiopsis Proteases (19) & (11) EP 2 258 838 A1 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.: 08.12.2010 Bulletin 2010/49 C12N 9/52 (2006.01) C12N 15/57 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) (2006.01) (2006.01) (21) Application number: 10180249.4 A23K 1/165 A23L 1/305 C11D 3/386 (2006.01) (22) Date of filing: 17.06.2005 (84) Designated Contracting States: (72) Inventors: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR • Lassen, Soeren Flensted HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR 3520, Farum (DK) • Sjoeholm, Carsten (30) Priority: 21.06.2004 DK 200400969 3450, Alleroed (DK) • Oestergaard, Peter Rahbek (83) Declaration under Rule 32(1) EPC (expert 2830, Virum (DK) solution) • Fischer, Morten 2950, Vedbæk (DK) (62) Document number(s) of the earlier application(s) in accordance with Art. 76 EPC: Remarks: 05748647.4 / 1 766 001 This application was filed on 27-09-2010 as a divisional application to the application mentioned (71) Applicant: Novozymes A/S under INID code 62. 2880 Bagsvaerd (DK) (54) Nocardiopsis proteases (57) Proteases derived from Nocardiopsis dasson- their recombinant production in various hosts, including villei subsp. dassonvillei DSM 43235,Nocardiopsis transgenic plants and non-human animals, and their use prasina DSM 15649, Nocardiopsis prasina (previously in animal feed and detergents. The proteases are acid- alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocar- stable, alkali-stable, and/or thermostable. diopsis alkaliphila DSM 44657 and Nocardiopsis lucen- tensis DSM 44048, as well as homologous proteases; EP 2 258 838 A1 Printed by Jouve, 75001 PARIS (FR) EP 2 258 838 A1 Description Field of the Invention 5 [0001] The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid se- quences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the polypep- tides, in particular the use of the polypeptides in animal feed, and detergents. 10 Background of the Invention [0002] Proteases derived from Nocardiopsis sp. NRRL 18262 and Nocardiopsis dassonvillei NRRL 18133 are dis- closed in WO 88/03947. The DNA and amino acid sequences of the protease derived fromNocardiopsis sp. NRRL 18262 are shown in DK application no. 1996 00013. WO 01/58276 discloses the use in animal feed of acid-stable 15 proteases related to the protease derived fromNocardiopsis sp. NRRL 18262, as well as a protease derived from Nocardiopsis alba DSM 14010. [0003] JP 2-255081-A discloses a protease derived from Nocardiopsis sp. strain OPC- 210 (FERM P- 10508), however without sequence information. The strain is no longer available, as the deposit was withdrawn. [0004] DD 200432|8 discloses a proteolytic preparation derived from Nocardiopsis dassonvillei strain ZIMET 43647, 20 however without sequence information. The strain appears to be no longer available. [0005] JP 2003284571-A discloses the amino acid sequence and the corresponding DNA sequence of a protease derived from Nocardiopsis sp. TOA-1 (FERM P-18676). The sequence has been entered in GENESEQP with no. ADF43564. [0006] It is an object of the present invention to provide alternative proteases, in particular for use in animal feed and/or 25 detergents. Summary of the Invention [0007] A number of proteases were cloned, purified and characterized. These proteases are designated as follows: 30 Protease L1a derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235 (see SEQ ID NOs. 1 and 2); protease L1b derived from Nocardiopsis prasina DSM 15649 (see SEQ ID NOs: 3 and 4); protease L1c derived from Nocardiopsis prasina (previously alba) DSM 14010 (see SEQ ID NOs: 5 and 6); protease L2a derived from Nocardiopsis sp. DSM 16424 (see SEQ ID NOs: 7 and 8); protease L2b derived from Nocardiopsis alkaliphila DSM 44657 (see SEQ ID NOs: 9 and 10); and protease L2c derived from Nocardiopsis lucentensis DSM 44048 (see SEQ ID NOs: 11 and 12). 35 [0008] In a first aspect, the invention relates to an isolated polypeptide having protease activity, selected from the group consisting of: (a) a polypeptide having an amino acid sequence which has a degree of identity to amino acids 1-192 of SEQ ID NO: 6 of at least 71.5%; (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under very high stringency conditions with (i) nucleotides 574-1149 of SEQ ID NO:5, (ii) a subsequence of (i) of at least 100 nucleotides, and/or (iii) a complementary strand of (i), or (ii); (c) a variant of the polypeptide having an amino acid 40 sequence of amino acids 1-192 of SEQ ID NO: 6 comprising a substitution, deletion, extension, and/or insertion of one or more amino acids; (d) an allelic variant of (a), or (b); and (e) a fragment of (a), (b), or (d) that has protease activity. [0009] In five alternative aspects, corresponding to the five sets of particular embodiments set forth at the end of the present description, the present invention also relates to: 45 An isolated polypeptide having protease activity, selected from the group consisting of: (a) a polypeptide having an amino acid sequence which has a degree of identity to amino acids 1-192 of SEQ ID NO: 4 of at least 69.9%; (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under low stringency conditions with (i) nucleotides 574-1149 of SEQ ID NO:3, (ii) a subsequence of (i) of at least 100 nucleotides, and/or (iii) a comple- mentary strand of (i), or (ii); (c) a variant of the polypeptide having an amino acid sequence of amino acids 1-192 50 of SEQ ID NO: 4 comprising a substitution, deletion, extension, and/or insertion of one or more amino acids; (d) an allelic variant of (a), or (b); and (e) a fragment of (a), (b), or (d) that has protease activity. An isolated polypeptide having protease activity, selected from the group consisting of: (a) a polypeptide having an amino acid sequence which has a degree of identity to amino acids 1-192 of SEQ ID NO: 2 of at least 75.1%; (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under low stringency conditions with (i) 55 nucleotides 568-1143 of SEQ ID NO: 1, (ii) a subsequence of (i) of at least 100 nucleotides, and/or (iii) a comple- mentary strand of (i), or (ii); (c) a variant of the polypeptide having an amino acid sequence of amino acids 1-192 of SEQ ID NO: 2 comprising a substitution, deletion, extension, and/or insertion of one or more amino acids; (d) an allelic variant of (a), or (b); and (e) a fragment of (a), (b), or (d) that has protease activity. 2 EP 2 258 838 A1 An isolated polypeptide having protease activity, selected from the group consisting of: (a) a polypeptide having an amino acid sequence which has a degree of identity to amino acids 1-189 of SEQ ID NO: 8 of at least 92.2%; (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under low stringency conditions with (i) nucleotides 586-1152 of SEQ ID NO: 7, (ii) a subsequence of (i) of at least 100 nucleotides, and/or (iii) a comple- 5 mentary strand of (i), or (ii); (c) a variant of the polypeptide having an amino acid sequence of amino acids 1-189 of SEQ ID NO: 8 comprising a substitution, deletion, extension, and/or insertion of one or more amino acids; (d) an allelic variant of (a), or (b); and (e) a fragment of (a), (b), or (d) that has protease activity. An isolated polypeptide having protease activity, selected from the group consisting of: (a) a polypeptide having an amino acid sequence which has a degree of identity to amino acids 1-189 of SEQ ID NO: 10 of at least 93.2%; (b) 10 a polypeptide which is encoded by a nucleic acid sequence which hybridizes under low stringency conditions with (i) nucleotides 586-1149 of SEQ ID NO: 9, (ii) a subsequence of (i) of at least 100 nucleotides, and/or (iii) a com- plementary strand of (i), or (ii); (c) a variant of the polypeptide having an amino acid sequence of amino acids 1-189 of SEQ ID NO: 10 comprising a substitution, deletion, extension, and/or insertion of one or more amino acids; (d) an allelic variant of (a), or (b); and (e) a fragment of (a), (b), or (d) that has protease activity. 15 An isolated polypeptide having protease activity, selected from the group consisting of: (a) a polypeptide having an amino acid sequence which has a degree of identity to amino acids 1-189 of SEQ ID NO: 12 of at least 83.3%; (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under low stringency conditions with (i) nucleotides 586-1152 of SEQ ID NO: 11, (ii) a subsequence of (i) of at least 100 nucleotides, and/or (iii) a complementary strand of (i), or (ii); (c) a variant of the polypeptide having an amino acid sequence of amino acids 20 1-189 of SEQ ID NO: 12 comprising a substitution, deletion, extension, and/or insertion of one or more amino acids; (d) an allelic variant of (a), or (b); and (e) a fragment of (a), (b), or (d) that has protease activity. [0010] The invention also relates to isolated nucleic acid sequences encoding the above polypeptides and to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and 25 using the polypeptides, in particular within animal feed, and detergents.
Load more

Recommended publications
  • Nocardiopsis Algeriensis Sp. Nov., an Alkalitolerant Actinomycete Isolated from Saharan Soil
    Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil Noureddine Bouras, Atika Meklat, Abdelghani Zitouni, Florence Mathieu, Peter Schumann, Cathrin Spröer, Nasserdine Sabaou, Hans-Peter Klenk To cite this version: Noureddine Bouras, Atika Meklat, Abdelghani Zitouni, Florence Mathieu, Peter Schumann, et al.. Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil. Antonie van Leeuwenhoek, Springer Verlag, 2015, 107 (2), pp.313-320. 10.1007/s10482-014-0329-7. hal- 01894564 HAL Id: hal-01894564 https://hal.archives-ouvertes.fr/hal-01894564 Submitted on 12 Oct 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. 2SHQ$UFKLYH7RXORXVH$UFKLYH2XYHUWH 2$7$2 2$7$2 LV DQ RSHQ DFFHVV UHSRVLWRU\ WKDW FROOHFWV WKH ZRUN RI VRPH 7RXORXVH UHVHDUFKHUVDQGPDNHVLWIUHHO\DYDLODEOHRYHUWKHZHEZKHUHSRVVLEOH 7KLVLVan author's YHUVLRQSXEOLVKHGLQhttp://oatao.univ-toulouse.fr/20349 2IILFLDO85/ http://doi.org/10.1007/s10482-014-0329-7 7RFLWHWKLVYHUVLRQ Bouras, Noureddine and Meklat, Atika and Zitouni, Abdelghani and Mathieu, Florence and Schumann, Peter and Spröer, Cathrin and Sabaou, Nasserdine and Klenk, Hans-Peter Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil. (2015) Antonie van Leeuwenhoek, 107 (2). 313-320. ISSN 0003-6072 $Q\FRUUHVSRQGHQFHFRQFHUQLQJWKLVVHUYLFHVKRXOGEHVHQWWRWKHUHSRVLWRU\DGPLQLVWUDWRU WHFKRDWDR#OLVWHVGLIILQSWRXORXVHIU Nocardiopsis algeriensis sp.
    [Show full text]
  • Nocardiopsis Fildesensis Sp. Nov., an Actinomycete Isolated from Soil
    International Journal of Systematic and Evolutionary Microbiology (2014), 64, 174–179 DOI 10.1099/ijs.0.053595-0 Nocardiopsis fildesensis sp. nov., an actinomycete isolated from soil Shanshan Xu, Lien Yan, Xuan Zhang, Chao Wang, Ge Feng and Jing Li Correspondence College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong, 266003, Jing Li PR China [email protected] or [email protected] A filamentous actinomycete strain, designated GW9-2T, was isolated from a soil sample collected from the Fildes Peninsula, King George Island, West Antarctica. The strain was identified using a polyphasic taxonomic approach. The strain grew slowly on most media tested, producing small amounts of aerial mycelia and no diffusible pigments on most media tested. The strain grew in the presence of 0–12 % (w/v) NaCl (optimum, 2–4 %), at pH 9.0–11.0 (optimum, pH 9.0) and 10– 37 6C (optimum, 28 6C). The isolate contained meso-diaminopimelic acid, no diagnostic sugars and MK-9(H4) as the predominant menaquinone. The major phospholipids were phosphatidylglycerol, phosphatidylcholine and phosphatidylmethylethanolamine. The major fatty acids were iso-C16 : 0, anteiso-C17 : 0,C18 : 1v9c, iso-C15 : 0 and iso-C17 : 0. DNA–DNA relatedness was 37.6 % with Nocardiopsis lucentensis DSM 44048T, the nearest phylogenetic relative (97.93 % 16S rRNA gene sequence similarity). On the basis of the results of a polyphasic study, a novel species, Nocardiopsis fildesensis sp. nov., is proposed. The type strain is GW9-2T (5CGMCC 4.7023T5DSM 45699T5NRRL B-24873T). The genus Nocardiopsis was initially described by Meyer 28 uC and stored at 4 uC.
    [Show full text]
  • Exploring and Exploiting the Microbial Resource of Hot and Cold Deserts
    BioMed Research International BIODESERT: Exploring and Exploiting the Microbial Resource of Hot and Cold Deserts Guest Editors: Ameur Cherif, George Tsiamis, Stéphane Compant, and Sara Borin BIODESERT: Exploring and Exploiting the Microbial Resource of Hot and Cold Deserts BioMed Research International BIODESERT: Exploring and Exploiting the Microbial Resource of Hot and Cold Deserts Guest Editors: Ameur Cherif, George Tsiamis, Stephane´ Compant, and Sara Borin Copyright © 2015 Hindawi Publishing Corporation. All rights reserved. This is a special issue published in “BioMed Research International.” All articles are open access articles distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Contents BIODESERT: Exploring and Exploiting the Microbial Resource of Hot and Cold Deserts,AmeurCherif, George Tsiamis, Stephane´ Compant, and Sara Borin Volume 2015, Article ID 289457, 2 pages The Date Palm Tree Rhizosphere Is a Niche for Plant Growth Promoting Bacteria in the Oasis Ecosystem, Raoudha Ferjani, Ramona Marasco, Eleonora Rolli, Hanene Cherif, Ameur Cherif, Maher Gtari, Abdellatif Boudabous, Daniele Daffonchio, and Hadda-Imene Ouzari Volume 2015, Article ID 153851, 10 pages Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land, Amel Khessairi, Imene Fhoula, Atef Jaouani, Yousra Turki, Ameur Cherif, Abdellatif Boudabous, Abdennaceur Hassen, and Hadda Ouzari
    [Show full text]
  • Evaluation of Antagonistic Activity of Actinobacteria in Saxicolous Lichens
    INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY ISSN Print: 1560–8530; ISSN Online: 1814–9596 16–339/2017/19–2–219–225 DOI: 10.17957/IJAB/15.0234 http://www.fspublishers.org Full Length Article Evaluation of Antagonistic Activity of Actinobacteria in Saxicolous Lichens Dongsheng Wang* and Haike Ren College of Life Science, Shanxi Normal University, Linfen, Shanxi 041000, China *For corresponding author: [email protected] Abstract Actinobacteria are important producers of novel bioactive compounds. New sources need to be explored for isolating previously unknown bioactive compound-producing actinobacteria. We evaluated the potential of saxicolous lichens as a natural source of novel bioactive actinobacterial species. Saxicolous lichen samples were collected from three climatic belts at different elevations (1600–3400 m) on Qin Mountain, Shaanxi Province, China. Actinobacteria were cultivated, enumerated, and isolated using serial dilution and spread-plate techniques under normal nutrient and oligotrophic conditions. Antimicrobial activity of actinobacterial isolates was analyzed using an agar block method against fifteen typical bacterial and fungal species and plant pathogens. The dominant isolates and isolates with broad-spectrum antagonistic activity were identified by 16S rRNA-based sequence analysis. Results showed that colony counts, total number of species, and the number of bioactive species of actinobacteria in saxicolous lichens were higher in elevation <3000 m than in elevation >3000 m. The dominant isolates were classified into 42 species of 10 genera. Antagonistic activities were detected in approximately 66% of the actinobacteria isolates. Of these, 42 isolates (28%) showed broad-spectrum antagonistic activity against ≥5 microorganisms tested. In conclusion, the saxicolous lichen possesses a high diversity of actinobacteria and serves as a natural source of bioactive compound-producing actinobacteria.
    [Show full text]
  • Genome-Based Taxonomic Classification of the Phylum
    ORIGINAL RESEARCH published: 22 August 2018 doi: 10.3389/fmicb.2018.02007 Genome-Based Taxonomic Classification of the Phylum Actinobacteria Imen Nouioui 1†, Lorena Carro 1†, Marina García-López 2†, Jan P. Meier-Kolthoff 2, Tanja Woyke 3, Nikos C. Kyrpides 3, Rüdiger Pukall 2, Hans-Peter Klenk 1, Michael Goodfellow 1 and Markus Göker 2* 1 School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom, 2 Department Edited by: of Microorganisms, Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Braunschweig, Martin G. Klotz, Germany, 3 Department of Energy, Joint Genome Institute, Walnut Creek, CA, United States Washington State University Tri-Cities, United States The application of phylogenetic taxonomic procedures led to improvements in the Reviewed by: Nicola Segata, classification of bacteria assigned to the phylum Actinobacteria but even so there remains University of Trento, Italy a need to further clarify relationships within a taxon that encompasses organisms of Antonio Ventosa, agricultural, biotechnological, clinical, and ecological importance. Classification of the Universidad de Sevilla, Spain David Moreira, morphologically diverse bacteria belonging to this large phylum based on a limited Centre National de la Recherche number of features has proved to be difficult, not least when taxonomic decisions Scientifique (CNRS), France rested heavily on interpretation of poorly resolved 16S rRNA gene trees. Here, draft *Correspondence: Markus Göker genome sequences
    [Show full text]
  • 中国科技论文在线 International Journal of Systematic and Evolutionary Microbiology (2001), 51, 357–363 Printed in Great Britain
    中国科技论文在线 http://www.paper.edu.cn International Journal of Systematic and Evolutionary Microbiology (2001), 51, 357–363 Printed in Great Britain Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae Xiao-Long Cui,1 Pei-Hong Mao,2 Min Zeng,3 Wen-Jun Li,1 Li-Ping Zhang,1 Li-Hua Xu1 and Cheng-Lin Jiang1 Author for correspondence: Cheng-Lin Jiang. Tel: j86 871 5034139. Fax: j86 871 5173878. e-mail: lihxu!ynu.edu.cn 1 Yunnan Institute of Actinomycete strain YIM 90002T (l CCTCC 99003T l CCRC 16284T) was isolated Microbiology, Yunnan from a soil sample collected from a salt lake in the west of China. The aerial University, Kunming 650091, Yunnan, China mycelium of this organism is well developed but not fragmented and, at maturity, forms short chains of spores. Spores in short chains are oval- to rod- 2 Institute of Microbiology, Xinjiang Agriculture shaped and have wrinkled surfaces. Substrate mycelium is branched with non- Academy of Sciences, fragmenting hyphae and forms single oval to round spores borne on Wulumuqi 830000, sporophores or dichotomously branching sporophores. Single spores have Xinjiang, China wrinkled surfaces. Single spores and spores in short chains are non-motile. 3 Food Industry Research & Strain YIM 90002T contains meso-diaminopimelic acid, DD-diaminopimelic acid, Development Institute, PO Box 246, Hsinchu, glycine, lysine and aspartic acid in its cell wall and has glucose, galactose, 300 Taiwan, China ribose, xylose, arabinose and mannose as whole-cell sugars (no diagnostic sugars). The phospholipids are phosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The major menaquinones are MK-9(H6), MK-10(H2) and MK-10(H4).
    [Show full text]
  • Cell Wall Teichoic Acids: Structural Diversity, Species Speci¢City in the Genus Nocardiopsis, and Chemotaxonomic Perspective
    FEMS Microbiology Reviews 25 (2001) 269^283 www.fems-microbiology.org Cell wall teichoic acids: structural diversity, species speci¢city in the genus Nocardiopsis, and chemotaxonomic perspective Irina B. Naumova a;*, Alexander S. Shashkov b, Elena M. Tul'skaya a, Galina M. Streshinskaya a, Yuliya I. Kozlova a, Nataliya V. Potekhina a, Lyudmila I. Evtushenko c, Erko Stackebrandt d a School of Biology, Lomonosov Moscow State University, Moscow 119899, Russia b Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prospect 47, Moscow 117913, Russia c VKM ^ All Russian Collection of Microorganisms, Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142292, Moscow Region, Russia d DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany Received 11 September 2000; received in revised form 10 November 2000; accepted 15 November 2000 Abstract Data on the structures of cell wall teichoic acids, the anionic carbohydrate-containing polymers, found in many Gram-positive bacteria have been summarized and the polymers of the actinomycete genus Nocardiopsis have been considered from the taxonomic standpoint. The structures of these polymers or their combinations have been demonstrated to be indicative of each of seven Nocardiopsis species and two subspecies, verified by the DNA^DNA relatedness data, and to correlate well with the grouping of the organisms based on 16S rDNA sequences. As each of the intrageneric taxa discussed is definable by the composition of teichoic acids, the polymers are considered to be valuable taxonomic markers for the Nocardiopsis species and subspecies. The 13C NMR spectra of the polymers, data on the products of their chemical degradation, and distinguishing constituents of whole cell walls derived from teichoic acids are discussed, which are useful for identification of certain polymers and members of the genus Nocardiopsis at the species and subspecies level in microbiological practice.
    [Show full text]
  • The Genus Nocardiopsis Represents a Phylogenetically Coherent Taxon and a Distinct Actinomycete Lineage: Prodosal of Nocardiomaceae Fam
    INTERNATIONAL JOURNALOF SYSTEMATICBACTERIOLOGY, Oct. 1996, p. 1088-1092 Vol. 46, No. 4 0020-7713/96/$04.00+0 Copyright 0 1996, International Union of Microbiological Societies The Genus Nocardiopsis Represents a Phylogenetically Coherent Taxon and a Distinct Actinomycete Lineage: ProDosal of Nocardiomaceae fam. nov. FRED A. RAINEY,” NAOMI WARD-RAINEY, REINER M. KROPPENSTEDT, AND ERKO STACKEBRANDT DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany The genus Nocardiopsis was shown to be phylogenetically coherent and to represent a distinct lineage within the radiation of the order Actinomycetales. The closest relatives of the genus Nocardiopsis are members of the genera Actinomadura, Thermomonospora,Streptosporangium, and Microtetraspora. The intrageneric structure of the genus Nucardiupsis is shown to consist of a highly related species group containing Nucardiupsis dassonvillei, Nocardiopsis alborubida, and Nocardiopsis antarctica and a second group of less highly related species compris- ing Nocardiopsis alba subsp. alba, Nocardiopsis alba subsp. prasina, and Nocardiopsis listeri. Nocardiopsis lucen- tensis occupies a position intermediate between the two species groups. The results of a 16s ribosomal DNA sequence analysis are generally consistent with the available chemotaxonomic, phenotypic, and DNA-DNA hybridization data. The phylogenetic position and the morpho- and chemotaxonomic properties of Nocardiopsis species support the creation of a family for the genus Nocardiopsis, Nocardiopsaceae fam. nov. Meyer described the genus Nocardiopsis for the species Ac- present. Diagnostic for all members of the genus Nocardiopsis tinomadura dassonvillei in 1976 on the basis of the morpholog- is the combination of 15 to 20% anteiso-C,,:, (14-methylhexa- ical characteristics and cell wall type of this organism (21).
    [Show full text]
  • Kent Academic Repository Kent Academic Repository Full Text Document (Pdf)
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Kent Academic Repository Kent Academic Repository Full text document (pdf) Citation for published version Asem, Mipeshwaree Devi and Salam, Nimaichand and Idris, Hamidah and Zhang, Xiao-Tong and Bull, Alan T. and Li, Wen-Jun and Goodfellow, Michael (2020) Nocardiopsis deserti sp. nov., isolated from a high altitude Atacama Desert soil. International Journal of Systematic and Evolutionary Microbiology . ISSN 1466-5026. DOI https://doi.org/10.1099/ijsem.0.004158 Link to record in KAR https://kar.kent.ac.uk/81008/ Document Version Publisher pdf Copyright & reuse Content in the Kent Academic Repository is made available for research purposes. Unless otherwise stated all content is protected by copyright and in the absence of an open licence (eg Creative Commons), permissions for further reuse of content should be sought from the publisher, author or other copyright holder. Versions of research The version in the Kent Academic Repository may differ from the final published version. Users are advised to check http://kar.kent.ac.uk for the status of the paper. Users should always cite the published version of record. Enquiries For any further enquiries regarding the licence status of this document, please contact: [email protected] If you believe this document infringes copyright then please contact the KAR admin team with the take-down information provided at http://kar.kent.ac.uk/contact.html TAXONOMIC DESCRIPTION Asem et al., Int. J. Syst. Evol. Microbiol. DOI 10.1099/ijsem.0.004158 Nocardiopsis deserti sp.
    [Show full text]
  • OUL6602.Pdf (10.82Mo)
    République Algérienne Démocratique et Populaire Ministère de l’Enseignement Supérieur et de la Recherche Scientifique Université Constantine 1 Faculté des Sciences de la Nature et de la Vie Département de Microbiologie N° d’ordre : 91/DS/2014 N° de série : 05/MBIO/2014 Thèse présentée par : OULMI Lamia En vue de l’obtention du Diplôme de : DOCTORAT EN SCIENCES Option : Biochimie et Microbiologie Appliquées ÉTUDE DES INFECTIONS CAUSÉES PAR LES ACTINOMYCÈTES AÉROBIE AUTRES QUE LES MYCOBACTÉRIES DANS LA RÉGION DE CONSTANTINE Soutenue le 22/09/2014 devant le jury Présidente : Pr. MIHOUBI Ilhem Univ. Constantine 1 Directeur de thèse : Pr. BOULAHROUF Abderrahmane Univ. Constantine 1 Examinateur : Pr. AITKAKI Zahia Univ. Constantine 3 Pr. KARAM Noureddine Univ. Essania-Oran Pr. MOULAHEM Tayeb Univ. Constantine 3 Année universitaire 2013/2014 DEDICACES A mes très chers parents En hommage à tous les sacrifices que vous avez consenti pour moi. Je vous remercie d'avoir fait de moi ce que je suis. Aucune dédicace, aucun mot, ne saurait exprimer réellement mon profond amour, mon respect et ma vive gratitude. Veuillez trouver dans ce travail le fruit de toutes vos espérances et vos sacrifices. A mon très cher époux qui m’a encouragée, qui m’a donné la force et la volonté de surmonter tous les obstacles et les difficultés. Merci pour m'avoir soutenu dans les moments les plus difficiles. A mon cher poussin, Moncef A ma famille, je dédie ce mémoire Quoique je fasse, je ne pourrais vous rendre ce que vous avez fait pour moi. Si j’en suis là, c’est bien grâce à vous.
    [Show full text]
  • Nocardiopsis Algeriensis Sp. Nov., an Alkalitolerant Actinomycete Isolated from Saharan Soil
    2SHQ$UFKLYH7RXORXVH$UFKLYH2XYHUWH 2$7$2 2$7$2 LV DQ RSHQ DFFHVV UHSRVLWRU\ WKDW FROOHFWV WKH ZRUN RI VRPH 7RXORXVH UHVHDUFKHUVDQGPDNHVLWIUHHO\DYDLODEOHRYHUWKHZHEZKHUHSRVVLEOH 7KLVLVan author's YHUVLRQSXEOLVKHGLQhttp://oatao.univ-toulouse.fr/20349 2IILFLDO85/ http://doi.org/10.1007/s10482-014-0329-7 7RFLWHWKLVYHUVLRQ Bouras, Noureddine and Meklat, Atika and Zitouni, Abdelghani and Mathieu, Florence and Schumann, Peter and Spröer, Cathrin and Sabaou, Nasserdine and Klenk, Hans-Peter Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil. (2015) Antonie van Leeuwenhoek, 107 (2). 313-320. ISSN 0003-6072 $Q\FRUUHVSRQGHQFHFRQFHUQLQJWKLVVHUYLFHVKRXOGEHVHQWWRWKHUHSRVLWRU\DGPLQLVWUDWRU WHFKRDWDR#OLVWHVGLIILQSWRXORXVHIU Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil • • • Noureddine Bouras Atika Meklat Abdelghani Zitouni • • • Florence Mathieu Peter Schumann Cathrin Spro¨er • Nasserdine Sabaou Hans-Peter Klenk Abstract An alkalitolerant actinomycete strain, des- (H4) and MK-11 (H4). The major fatty acids were T ignated B32 , was isolated from a Saharan soil sample found to be iso-C16:0 and anteiso-C15:0. The diagnostic collected from Adrar province (South of Algeria), and phospholipids detected were phosphatidylcholine, then investigated using a polyphasic taxonomic phosphatidylmethylethanolamine, diphosphatidyl- approach. The strain was observed to produce short glycerol, phosphatidylglycerol and phosphatidyletha- chains of spores on the dichotomous branched aerial nolamine. The chemotaxonomic properties of strain mycelium and formed a fragmented substrate myce- B32T are consistent with those shared by members of lium. The optimum NaCl concentration for growth the genus Nocardiopsis. 16S rRNA gene sequence was found to be 0–5 % (w/v) and the optimum growth analysis indicated that strain B32T is most closely temperature and pH were found to be 25–35 °C and related to Nocardiopsis alba DSM 43377T (98.7 %), 7.0–10.0 °C, respectively.
    [Show full text]
  • Polyphasic Taxonomy of Thermophilic
    Polyphasic Taxonomy of Thermophilic Actinomycetes Thesis submitted in accordance with the requirement of the University of Newcastleupon Tyne, U. K. for the Degreeof Doctor of Philosophy by Bongcheol Kim (B. Sc., Seoul National University, Seoul, Korea) Department of Agricultural and Environmental Science, University of Newcastle upon Tyne, U. K. February1999 NEWCASTLE-UNIVERSITY-LIBRARY -------------------------- 098 14280 5 --------- l-ý'ýý7 ýý ý__I Q-1çovJ ýý ýý ýýý ý ý'ý ý"ýý -4/ t-ýt? Dedicated to my loving father and mother Acknowledgements I would like to express my sincere gratitude to my supervisor Professor Michael Goodfellow for his guidance, patience and advice during the preparation of the thesis and, most of all, for his care and concern about my life in Newcastle. I am also grateful to Professor David E. Minnikin for his sound advice on the chemotaxonomic work and to Dr. Alan C. Ward for his time and help with the computing. I am also indebted to Professor Yung Chi] Hah for introducing me to microbial systematics and for his interest in my work. Special thanks go to Dr. Nevzat Sahin whose project provided the basis for much of my research work. I am also very grateful to Drs. Seung Bum Kim and Jongsik Chun for their invaluableadvice on my project. I am grateful to Professor Marian Mordarski and Dr. Jolanta Zakrzewska- Czerwinska for arranging my stay in Wroclaw, Poland; to Maria Guzik and Bashia Lis for their wonderful assistance with the DNA: DNA hybridisation experiments and to Dr. Stefan Rowinski for his friendship and his `passionate' tours of Poland.
    [Show full text]