DOCSLIB.ORG

Download (8MB)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Download (8MB) https://theses.gla.ac.uk/ Theses Digitisation: https://www.gla.ac.uk/myglasgow/research/enlighten/theses/digitisation/ This is a digitised version of the original print thesis. Copyright and moral rights for this work are retained by the author A copy can be downloaded for personal non-commercial research or study, without prior permission or charge This work cannot be reproduced or quoted extensively from without first obtaining permission in writing from the author The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the author When referring to this work, full bibliographic details including the author, title, awarding institution and date of the thesis must be given Enlighten: Theses https://theses.gla.ac.uk/ [email protected] BACTERIAL ASSOCIATIONS WITH COMMERCIALLY IMPORTANT MARINE BIVALVES by Eileen Lane Thesis presented for the Degree of Doctor of Philosophy in the Faculty of Science, University of Glasgow Department of Microbiology, May 1997 Copyright © ; Eileen Lane. ProQuest Number: 10391365 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a com plete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest ProQuest 10391365 Published by ProQuest LLO (2017). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States C ode Microform Edition © ProQuest LLO. ProQuest LLO. 789 East Eisenhower Parkway P.Q. Box 1346 Ann Arbor, Ml 48106- 1346 University o f ÇCosyoïV .S” GlASG0y;UM(VE8aiV LIBmV Dedicated to my mother and late father, Angela and Morgan Lane. To my family and friends, thank you for all the kindness that you have shown to me. 11 Many a mickle maks a muckle (Author unknown) Ill ACKNOWLEDGEMENTS I would like to thank Dr. Thomas H. Birkbeck for allowing me to do this project and for his help, guidance and patience throughout. I am also endebted to Prof. Alastair C. Wardlaw for his invaluable advice and assistance with the text. I am grateful to Mr. John Bayes, Seasalter Shellfish (Whitsable) Limited, Kent, Mr. Mark Dravers, Guernsey Sea Farms Limited, Guernsey, Channel Islands and Dr. Susan Utting, (Ministry of Agriculture, Fisheries and Food) Conwy, Wales, for the provision of specimens. I would also like to thank Dr. Jean-Louis Nicolas, Ifremer, Brest, Dr. Alicia E. Toranzo and Dr. Juan L. Barja, Universidad de Santiago de Compostela, Departamento de Microbiologia, Santiago, Spain and Dr. Pauline Munro, University of Glasgow, Scotland, for the provision of bacterial isolates. Thank you to Mr. John Redshaw for his assistance with the oyster embryo assay. To the members of the Microbiology Department and my friends, thank you all for your support, encouragement and friendship. Finally I would like to thank the Directorate General XIV of the European Community for providing the financial support for this project under the FAR programme. IV LIST OF ABBREVIATIONS A Absorbance BRD Brown Ring Disease C Control c.f.u. Colony forming units CAM Calcein-AM CLED Cysteine Lactose Electrolyte Deficient Medium C.P.D. Critical point dryer CT Ciliostatic toxin Da Dalton DMSO Dimethyl sulphoxide D-PBS Dulbecco's phosphate buffered saline ETHD Ethidium Homodimer FH Filtered haemolymph FSW Filtered sea water h Hour g Gram 1 Litre LD50 Lethal dose 50 LPS Lipopolysaccharide M.W. Molecular weight MA Marine agar MB Marine broth min Minute ml Millilitre mM Millimolar MOF Marine Oxidative/Fermentative Medium MPS Mytilus edulis physiological saline. NCIMB National Collection of Industrial and Marine Bacteria NMWL Nominal molecular weight limit OD Optical density ONPG 0-Nitrophenyl-^-D-galactopyranoside OVVD Oyster Velar Virus Disease PEG Polyethylene glycol PMS Phenazine methosulphate PT Pertussis toxin SDS Sodium dodecyl sulphate SEM Scanning Electron Microscopy Sj Jaccard Similarity Coefficient Sp. Speciea SSM Simple Matching Similarity Coefficient TCBS Thiosulphate Citrate Bile Salts Sucrose U Units \xg Microgram (il Microlitre jxm Micrometre UPGMA Unweighted pair group method with averages VP V oges-Proskauer v/v Volume per volume w/v Weight per volume XTT 2, 3 -bis [2-methoxy-4-nitro-5-sulfophenyl]-2-H-tetrazolium-5 carboxanilide inner salt VI INDEX OF FIGURES Figure number Page 1 Outline of hatchery culture. 5 2 Phase contrast photographs (Ai and Bi, x 40) and scanning electron micrographs (Aii, x 200), (Bii, x 100) of oyster larvae {Ostrea edulis), (A) treated or (B) not treated with Benzalkonium chloride. 44 3 Cluster analysis of bacterial isolates from Guernsey, analysed by Jaccard Similarity Coefficient (S j) linked with UPGMA method and the programme. Bacterial Identifier (Bryant et al, 1986). A reference strain V006 (V. anguillarum 6) was included. 55 4 Cluster analysis of bacterial isolates from Guernsey, analysed by Simple Matching, Similarity Coefficient (SsM) linked with UPGMA method and the programme. Bacterial Identifier. 56 5 Cluster analysis of bacterial isolates from Conwy and Reculver analysed by Jaccard Similarity Coefficient (Sj) linked with the UPGMA method and the programme. Bacterial Identifier. 58 6 Cluster analysis of bacterial isolates from Conwy and Reculver, analysed by Simple Matching, Similarity Coefficient (SsM) linked with the UPGMA method and the programme. Bacterial Identifier. 59 7 Cluster analysis of bacterial isolates from Conwy, Guernsey and Reculver analysed by Jaccard, Similarity Coefficient (Sj) linked with the UPGMA method and the programme. Bacterial Identifier. 63 ï V il Figure number Page 8 Cluster analysis of bacterial isolates from Conwy, Guernsey and Reculver, analysed by Simple Matching, Similarity Coefficient (SsM) linked with the UPGMA method and the programme. Bacterial Identifier. 64 9 A simplified dendrogram of 113 isolates originating from France (16), Spain (54), United Kingdom (34) and reference bacterial strains (9) as produced by a group of French researchers. 65 10 The effects of bacteria on oyster larvae. 68 11 Visual observation of haemocyte survival indicated by the ability of the haemocyte to take up neutral red dye, after incubation with various bacteria suspended in filtered sea water and at different c.f.u. per haemocyte. 71 12 The effect of incubation of Mytilus haemocytes with various bacteria for 3 h at 20 ^C, deteiTOined by neutral red absorbance at 540 nm or fluorescence at 400 nm. Bacteria tested were suspended in filtered sea water. 73 13 The effect of bacteria on Mytilus haemocytes. XTT was used to determine the number of live haemocytes after incubation with different bacteria for . 45 min at 20 ^C. 74 14 Antibiotic toxicity screening using Mytilus haemocytes, XTT and antibiotic ■ concentrations ranging between 0.01 and 1 mg/0.1 ml. 76 15 The effect of different vibrios on Mytilus haemocytes as determined using calcein-AM. 78 16 Effects of increasing ethanol concentration on the viability of Mytilus haemocytes assessed by uptake of ethidium homodimer. 79 Vlll Figure number Page 17 Effect of haemocyte suspension volume and length of incubation with ETHD on the fluorescence signal. 81 18 The effect of increasing bacteria/haemocyte ratios on the toxicity of bacteria to haemocytes from Mytilus edulis. 82 19 The effect of 100 and 1000 c.f.u. per haemocyte, on the toxicity of Vibrio spp. (V6, V I197, V1338, V1339, V1340, V2166, V2981, V5679, V91079, VBl or VB51) or P I-1-1, suspended in FSW and when screened for their toxicity to Mytilus haemocytes using ethidium homodimer. 83 20 The effect of incubation time on the toxicity of V I339 or V2981 to Mytilus haemocytes. Haemocytes were incubated for (a) 3 h or (b) 6 h with V I339 or V2981 (50, 100 or 500 / haemocyte) and the subsequent viability of haemocytes assessed using ethidium homodimer. 85 21 The effect of caffeine and buffers on Myri/w.s'haemocytes. 86 22 The effect of incubation media, FSW (22 a) or FH (22 b), on the toxicity of V I339 to Mytilus haemocytes at 10 to 1000 c.f.u. per haemocyte and incubated for 3 h at 20 ^C. 87 23 The toxic effect of V1338, V1339, V2165, V2981 or V6 to Mytilus haemocytes when suspended in FH at bacterial ratios of 0.1 to 100 c.f.u. per haemocyte and incubated for 3 h at 20 88 24 The effect of Pseudomonas 1-1-1 or V2981, suspended in FH or FSW, on haemocytes of Mytilus edulis. 90 25 Kinetics of rounding of Mytilus haemocytes on interaction with V. anguillarum 2981 or V, anguillarum A7. 92 IX Figure number Page 26 Toxicity of bacterial isolates from various shellfish hatcheries to haemocytes at 50 c.f.u. per haemocyte. 97 27 Toxicity of 9 test bacteria to haemocytes of Mercenaria mercenaria. Tapes decussatus and Tapes semidecussatus. 102 28 Toxicity of 9 test bacteria to haemocytes of Crassostrea gigas, Ostrea edulis, Mytilus edulis and Pecten maximus. 106 29 Effect of incubation temperature on the toxicity of Vibrio anguillarum 2981 to haemocytes of Mytilus edulis. 107 30 Effect of bacterial and peptone concentrations on the toxicity of the V. anguillarum 29^1 to haemocytes. 109 31 SDS-PAGE analysis of filtered haemolymph before and after absorption with V. anguillarum 2981 lipopolysaccharide. 111 32 Effect of trypsin treatment of filtered haemolymph on the toxic effect of V. anguillarum 2981 to Mytilus haemocytes. 114 33 The effect of FSW, untreated FH or bacteria-treated FH as diluents, on the toxicity of V. anguillarum 2981 to Mytilus haemocytes. 115 34 Toxicity to Mytilus haemocytes of bacteria-free culture filtrates. 118 35 Cluster analysis of bacterial isolates from turbot hatcheries. 125 36 Vésiculation of haemocytes after incubation with bacterial isolate NT 17.
Load more

Recommended publications
  • Characterization of N-Acyl Homoserine Lactones in Vibrio Tasmaniensis LGP32 by a Biosensor-Based UHPLC-HRMS/MS Method
    sensors Article Characterization of N-Acyl Homoserine Lactones in Vibrio tasmaniensis LGP32 by a Biosensor-Based UHPLC-HRMS/MS Method Léa Girard 1, Élodie Blanchet 1, Laurent Intertaglia 2, Julia Baudart 1, Didier Stien 1, Marcelino Suzuki 1, Philippe Lebaron 1 and Raphaël Lami 1,* 1 Sorbonne Universités, UPMC Univ Paris 6, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, F-66650 Banyuls/Mer, France; [email protected] (L.G.); [email protected] (É.B.); [email protected] (J.B.); [email protected] (D.S.); [email protected] (M.S.); [email protected] (P.L.) 2 Sorbonne Universités, UPMC Univ Paris 06, CNRS, Observatoire Océanologique de Banyuls (OOB), F-66650 Banyuls/Mer, France; [email protected] * Correspondence: [email protected]; Tel.: +33-430-192-468 Academic Editors: Jean-Louis Marty, Silvana Andreescu and Akhtar Hayat Received: 4 April 2017; Accepted: 17 April 2017; Published: 20 April 2017 Abstract: Since the discovery of quorum sensing (QS) in the 1970s, many studies have demonstrated that Vibrio species coordinate activities such as biofilm formation, virulence, pathogenesis, and bioluminescence, through a large group of molecules called N-acyl homoserine lactones (AHLs). However, despite the extensive knowledge on the involved molecules and the biological processes controlled by QS in a few selected Vibrio strains, less is known about the overall diversity of AHLs produced by a broader range of environmental strains. To investigate the prevalence of QS capability of Vibrio environmental strains we analyzed 87 Vibrio spp. strains from the Banyuls Bacterial Culture Collection (WDCM911) for their ability to produce AHLs.
    [Show full text]
  • Pecten Maximus) Larvae
    Vibrio pectenicida sp. nov., a pathogen of scallop (Pecten maximus) larvae. Christophe Lambert, Jean-Louis Nicolas, Valérie Cilia, Sophie Corre To cite this version: Christophe Lambert, Jean-Louis Nicolas, Valérie Cilia, Sophie Corre. Vibrio pectenicida sp. nov., a pathogen of scallop (Pecten maximus) larvae.. International Journal of Systematic Bacteriology, So- ciety for General Microbiology, 1998, 48 (2), pp.481-487. 10.1099/00207713-48-2-481. hal-00447706 HAL Id: hal-00447706 https://hal.archives-ouvertes.fr/hal-00447706 Submitted on 1 Feb 2010 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Vibrio pectenicida sp. nov., a pathogen of scallop ( Pecten maximus ) larvae C. Lambert 1, J. L. Nicolas 1, V. Cilia 1 and S. Corre 2 Author for correspondence: J. L. Nicolas. Tel: + 33 2 98 22 43 99. Fax: +33 2 98 22 45 47. e-mail: [email protected] 1 Laboratoire de Physiologie des Invertébrés Marins, DRV/A, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France 2 Micromer Sarl, ZI du vernis, Brest, France Abstract : Five strains were isolated from moribund scallop ( Pecten maximus ) larvae over 5 years (1990–1995) during outbreaks of disease in a hatchery (Argenton, Brittany, France).
    [Show full text]
  • Vibrio Spp. in the German Bight
    VIBRIO SPP. IN THE GERMAN BIGHT by Sonja Oberbeckmann A thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Marine Microbiology Approved, Thesis Committee: Prof. Dr. Karen H. Wiltshire Jacobs University Bremen Alfred Wegener Institute for Marine and Polar Research Dr. Gunnar Gerdts Alfred Wegener Institute for Marine and Polar Research Dr. Antje Wichels Alfred Wegener Institute for Marine and Polar Research Prof. Dr. Matthias Ullrich Jacobs University Bremen Date of Defense: March 7, 2011 School of Engineering and Science TABLE OF CONTENTS INTRODUCTION 1 RESEARCH AIMS 8 OUTLINE 10 CHAPTER I 13 A polyphasic approach for the differentiation of environmental Vibrio isolates from temperate waters. CHAPTER II 41 Occurrence of Vibrio parahaemolyticus and Vibrio alginolyticus in the German Bight over a seasonal cycle. CHAPTER III 67 Seasonal dynamics and predictive modeling of a Vibrio community in coastal waters of the North Sea. GENERAL DISCUSSION 93 SUMMARY 103 REFERENCES 105 ACKNOWLEDGEMENTS 128 DECLARATION 131 INTRODUCTION INTRODUCTION Region in focus: The North Sea The North Sea is a semi-enclosed shelf sea, which stretches between the Northern European mainland and the Atlantic Ocean. It has an average depth of 95 m with a maximum of 750 m in the south of Norway. The average water temperature is 6°C in winter and 17°C in summer. North Sea water has a salinity of 34 – 35, but the salinity around river discharges is much lower (MUMM, 2000, Howarth, 2001). The North Sea is constantly in motion in response to tides, wind and the inflow of Atlantic Ocean water or freshwater, with tides representing the strongest influence.
    [Show full text]
  • New Vibrio Species Associated to Molluscan Microbiota: a Review
    REVIEW ARTICLE published: 02 January 2014 doi: 10.3389/fmicb.2013.00413 New Vibrio species associated to molluscan microbiota: a review Jesús L. Romalde*, Ana L. Diéguez, Aide Lasa and Sabela Balboa Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela, Spain Edited by: The genus Vibrio consists of more than 100 species grouped in 14 clades that are widely Daniela Ceccarelli, University of distributed in aquatic environments such as estuarine, coastal waters, and sediments. Maryland, USA A large number of species of this genus are associated with marine organisms like fish, Reviewed by: molluscs and crustaceans, in commensal or pathogenic relations. In the last decade, more Patrick Monfort, Centre National de la Recherche Scientifique, France than 50 new species have been described in the genus Vibrio, due to the introduction of Eva Benediktsdóttir, University of new molecular techniques in bacterial taxonomy, such as multilocus sequence analysis or Iceland, Iceland fluorescent amplified fragment length polymorphism. On the other hand, the increasing *Correspondence: number of environmental studies has contributed to improve the knowledge about the Jesús L. Romalde, Departamento de familyVibrionaceae and its phylogeny. Vibrio crassostreae, V. breoganii, V. celticus are some Microbiología y Parasitología, CIBUS-Facultad de Biología, of the new Vibrio species described as forming part of the molluscan microbiota. Some Universidad de Santiago de of them have been associated with mortalities of different molluscan species, seriously Compostela, Campus Vida s/n, affecting their culture and causing high losses in hatcheries as well as in natural beds. Santiago de Compostela 15782, Spain e-mail: [email protected] For other species, ecological importance has been demonstrated being highly abundant in different marine habitats and geographical regions.
    [Show full text]
  • Vibrio Fortis Sp. Nov. and Vibrio Hepatarius Sp. Nov., Isolated from Aquatic Animals and the Marine Environment
    International Journal of Systematic and Evolutionary Microbiology (2003), 53, 1495–1501 DOI 10.1099/ijs.0.02658-0 Vibrio fortis sp. nov. and Vibrio hepatarius sp. nov., isolated from aquatic animals and the marine environment F. L. Thompson,1,2 C. C. Thompson,1,2 B. Hoste,2 K. Vandemeulebroecke,2 M. Gullian3 and J. Swings1,2 Correspondence 1,2Laboratory for Microbiology1 and BCCM/LMG Bacteria Collection2, Ghent University, K. L. F. L. Thompson Ledeganckstraat 35, Ghent 9000, Belgium [email protected] 3National Center for Marine and Aquaculture Research, Guayaquil, Ecuador In this study, the taxonomic positions of 19 Vibrio isolates disclosed in a previous study were evaluated. Phylogenetic analysis based on 16S rDNA sequences partitioned these isolates into groups that were closely related (98?8–99?1 % similarity) to Vibrio pelagius and Vibrio xuii, respectively. DNA–DNA hybridization experiments further showed that these groups had <70 % similarity to other Vibrio species. Two novel Vibrio species are proposed to accommodate these groups: Vibrio fortis sp. nov. (type strain, LMG 21557T=CAIM 629T) and Vibrio hepatarius sp. nov. (type strain, LMG 20362T=CAIM 693T). The DNA G+C content of both novel species is 45?6 mol%. Useful phenotypic features for discriminating V. fortis and V. hepatarius from other Vibrio species include production of indole and acetoin, utilization of cellobiose, fermentation of amygdalin, melibiose and mannitol, b-galactosidase and tryptophan deaminase activities and fatty acid composition. INTRODUCTION haemolymph of healthy L. vannamei. Certain Vibrio strains have been reported to be potential probiotics for this Vibrios are among the most abundant culturable microbes shrimp (Gomez-Gil et al., 1998, 2000, 2002).
    [Show full text]
  • Bacterial Secretion of D-Arginine Controls Environmental Microbial Biodiversity
    The ISME Journal (2018) 12, 438–450 © 2018 International Society for Microbial Ecology All rights reserved 1751-7362/18 www.nature.com/ismej ORIGINAL ARTICLE Bacterial secretion of D-arginine controls environmental microbial biodiversity Laura Alvarez1, Alena Aliashkevich1, Miguel A de Pedro2 and Felipe Cava1 1Department of Molecular Biology, Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, Umeå University, Umeå, Sweden and 2Centro de Biología Molecular ‘Severo Ochoa’, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Spain Bacteria face tough competition in polymicrobial communities. To persist in a specific niche, many species produce toxic extracellular effectors to interfere with the growth of nearby microbes. These effectors include the recently reported non-canonical D-amino acids (NCDAAs). In Vibrio cholerae, the causative agent of cholera, NCDAAs control cell wall integrity in stationary phase. Here, an analysis of the composition of the extracellular medium of V. cholerae revealed the unprecedented presence of D-Arg. Compared with other D-amino acids, D-Arg displayed higher potency and broader toxicity in terms of the number of bacterial species affected. Tolerance to D-Arg was associated with mutations in the phosphate transport and chaperone systems, whereas D-Met lethality was suppressed by mutations in cell wall determinants. These observations suggest that NCDAAs target different cellular processes. Finally, even though virtually all Vibrio species are tolerant to D-Arg, only a few can produce this D-amino acid. Indeed, we demonstrate that D-Arg may function as part of a cooperative strategy in vibrio communities to protect non-producing members from competing bacteria.
    [Show full text]
  • Deep-Sea Hydrothermal Vent Bacteria Related to Human Pathogenic Vibrio
    Deep-sea hydrothermal vent bacteria related to human PNAS PLUS pathogenic Vibrio species Nur A. Hasana,b,c, Christopher J. Grima,c,1, Erin K. Lippd, Irma N. G. Riverae, Jongsik Chunf, Bradd J. Haleya, Elisa Taviania, Seon Young Choia,b, Mozammel Hoqg, A. Christine Munkh, Thomas S. Brettinh, David Bruceh, Jean F. Challacombeh, J. Chris Detterh, Cliff S. Hanh, Jonathan A. Eiseni, Anwar Huqa,j, and Rita R. Colwella,b,c,k,2 aMaryland Pathogen Research Institute, cUniversity of Maryland Institute for Advanced Computer Studies, and jInstitute for Applied Environmental Health, University of Maryland, College Park, MD 20742; bCosmosID, College Park, MD 20742; dEnvironmental Health Science, College of Public Health, University of Georgia, Athens, GA 30602; eDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, CEP 05508-900 São Paulo, Brazil; fSchool of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea; gDepartment of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh; hGenome Science Group, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545; iUniversity of California Davis Genome Center, Davis, CA 95616; and kBloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21205 Contributed by Rita R. Colwell, April 15, 2015 (sent for review September 5, 2014; reviewed by John Allen Baross, Richard E. Lenski, and Carla Pruzzo) Vibrio species are both ubiquitous and abundant in marine coastal of vibrios, and suggested Vibrio populations generally comprise waters, estuaries, ocean sediment, and aquaculture settings world- approximately 1% (by molecular techniques) of the total bac- wide. We report here the isolation, characterization, and genome terioplankton in estuaries (19), in contrast to culture-based studies sequence of a novel Vibrio species, Vibrio antiquarius, isolated from demonstrating that vibrios can comprise up to 10% of culturable a mesophilic bacterial community associated with hydrothermal marine bacteria (20).
    [Show full text]
  • Effects of Biofloc Promotion on Water Quality, Growth, Biomass Yield and Heterotrophic Community in Litopenaeus Vannamei (Boone, 1931) Experimental Intensive Culture
    Italian Journal of Animal Science ISSN: (Print) 1828-051X (Online) Journal homepage: http://www.tandfonline.com/loi/tjas20 Effects of Biofloc Promotion on Water Quality, Growth, Biomass Yield and Heterotrophic Community in Litopenaeus Vannamei (Boone, 1931) Experimental Intensive Culture Irasema E. Luis-Villaseñor, Domenico Voltolina, Juan M. Audelo-Naranjo, María R. Pacheco-Marges, Víctor E. Herrera-Espericueta & Emilio Romero- Beltrán To cite this article: Irasema E. Luis-Villaseñor, Domenico Voltolina, Juan M. Audelo-Naranjo, María R. Pacheco-Marges, Víctor E. Herrera-Espericueta & Emilio Romero-Beltrán (2015) Effects of Biofloc Promotion on Water Quality, Growth, Biomass Yield and Heterotrophic Community in Litopenaeus Vannamei (Boone, 1931) Experimental Intensive Culture, Italian Journal of Animal Science, 14:3, 3726 To link to this article: http://dx.doi.org/10.4081/ijas.2015.3726 ©Copyright I.E. Luis-Villaseñor et al. Published online: 17 Feb 2016. Submit your article to this journal Article views: 193 View related articles View Crossmark data Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=tjas20 Download by: [93.179.90.222] Date: 01 July 2016, At: 06:04 Italian Journal of Animal Science 2015; volume 14:3726 PAPER nated in both systems; in both some isolates Effects of biofloc promotion were potential pathogens, and diversity was Corresponding author: Dr. Juan M. Audelo- on water quality, growth, higher in the control than in the BFT treat- Naranjo, Universidad Autónoma de Sinaloa, ment. The advantages of BFT technology are Facultad de Ciencias del Mar, Mazatlán, 610 biomass yield and confirmed by the significantly lower TAN and Sinaloa, Mexico.
    [Show full text]
  • Vibrio Aerogenes Sp. Nov., a Facultatively Anaerobic Marine Bacterium That Ferments Glucose with Gas Production
    International Journal of Systematic and Evolutionary Microbiology (2000), 50, 321–329 Printed in Great Britain Vibrio aerogenes sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production Wung Yang Shieh, Aeen-Lin Chen† and Hsiu-Hui Chiu Author for correspondence: Wung Yang Shieh. Tel. ­886 2 23636040 ext. 417 Fax: ­886 2 23626092. e-mail: winyang!ms.cc.ntu.edu.tw Institute of Oceanography, A mesophilic, facultatively anaerobic, marine bacterium, designated strain National Taiwan FG1T, was isolated from a seagrass bed sediment sample collected from University, PO Box 23-13, Taipei, Taiwan Nanwan Bay, Kenting National Park, Taiwan. Cells grown in broth cultures were motile, Gram-negative rods; motility was normally achieved by two sheathed flagella at one pole of the cell. Strain FG1T required NaM for growth, and exhibited optimal growth at 30–35 SC, pH 6–7 and about 4% NaCl. It grew anaerobically by fermenting glucose and other carbohydrates with production of various organic acids, including acetate, lactate, formate, malate, oxaloacetate, propionate, pyruvate and succinate, and the gases CO2 and H2. The strain did not require either vitamins or other organic growth factors for growth. Its DNA GMC content was 45<9 mol%. It contained C12:0 as the most abundant cellular fatty acid. Characterization data, together with the results of a 16S rDNA-based phylogenetic analysis, indicate that strain FG1T represents a new species of the genus Vibrio. Thus, the name Vibrio aerogenes sp. nov. is proposed for this new bacterium. The type strain is FG1T (¯ ATCC 700797T ¯ CCRC 17041T). Keywords: Vibrio aerogenes sp.
    [Show full text]
  • Vibrio Breoganii Sp. Nov., a Non-Motile, Alginolytic, Marine Bacterium Within the Vibrio Halioticoli Clade
    International Journal of Systematic and Evolutionary Microbiology (2009), 59, 1589–1594 DOI 10.1099/ijs.0.003434-0 Vibrio breoganii sp. nov., a non-motile, alginolytic, marine bacterium within the Vibrio halioticoli clade Roxana Beaz Hidalgo,1 Ilse Cleenwerck,2 Sabela Balboa,1 Susana Prado,1 Paul De Vos2 and Jesu´s L. Romalde1 Correspondence 1Departamento de Microbiologı´a y Parasitologı´a, Centro de Investigaciones Biolo´gicas (CIBUS)- Jesu´s L. Romalde Facultad de Biologı´a, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, [email protected] Spain 2BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ghent, Belgium Seven non-motile, facultatively anaerobic, alginolytic marine bacteria were isolated from the cultured clams Ruditapes philippinarum and Ruditapes decussatus. Phylogenetic analysis based on 16S rRNA gene sequences showed that these marine bacteria were closely related to the recently described species Vibrio comitans, Vibrio rarus and Vibrio inusitatus (¢99.0 % sequence similarity). Phylogenetic analysis based on the housekeeping genes rpoA, recA and atpA grouped the isolates together and allocated them to the Vibrio halioticoli species group. Amplified fragment length polymorphism DNA fingerprinting also grouped them together and enabled them to be differentiated from recognized species of the V. halioticoli clade. DNA–DNA hybridizations showed that the isolates belonged to a novel species; phenotypic features such as the ability to grow at 4 6C and in the presence of 6 % NaCl also enabled them to be separated from other species. The DNA G+C content of RD 15.11T is 44.4 mol%. The genotypic and phenotypic data showed that the isolates represent a novel species in the V.
    [Show full text]
  • Deep-Sea Hydrothermal Vent Bacteria Related to Human Pathogenic Vibrio
    Deep-sea hydrothermal vent bacteria related to human PNAS PLUS pathogenic Vibrio species Nur A. Hasana,b,c, Christopher J. Grima,c,1, Erin K. Lippd, Irma N. G. Riverae, Jongsik Chunf, Bradd J. Haleya, Elisa Taviania, Seon Young Choia,b, Mozammel Hoqg, A. Christine Munkh, Thomas S. Brettinh, David Bruceh, Jean F. Challacombeh, J. Chris Detterh, Cliff S. Hanh, Jonathan A. Eiseni, Anwar Huqa,j, and Rita R. Colwella,b,c,k,2 aMaryland Pathogen Research Institute, cUniversity of Maryland Institute for Advanced Computer Studies, and jInstitute for Applied Environmental Health, University of Maryland, College Park, MD 20742; bCosmosID, College Park, MD 20742; dEnvironmental Health Science, College of Public Health, University of Georgia, Athens, GA 30602; eDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, CEP 05508-900 São Paulo, Brazil; fSchool of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea; gDepartment of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh; hGenome Science Group, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545; iUniversity of California Davis Genome Center, Davis, CA 95616; and kBloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21205 Contributed by Rita R. Colwell, April 15, 2015 (sent for review September 5, 2014; reviewed by John Allen Baross, Richard E. Lenski, and Carla Pruzzo) Vibrio species are both ubiquitous and abundant in marine coastal of vibrios, and suggested Vibrio populations generally comprise waters, estuaries, ocean sediment, and aquaculture settings world- approximately 1% (by molecular techniques) of the total bac- wide. We report here the isolation, characterization, and genome terioplankton in estuaries (19), in contrast to culture-based studies sequence of a novel Vibrio species, Vibrio antiquarius, isolated from demonstrating that vibrios can comprise up to 10% of culturable a mesophilic bacterial community associated with hydrothermal marine bacteria (20).
    [Show full text]
  • Vibrio Mytili Sp. Nov., from Mussels MAR~A-JESUS PUJALTE,'" MARGARITA ORTIGOSA,~MARIA-CAMINO URDACI,~ ESPERANZA GARAY,L and PATRICK A
    INTERNATIONAL JOURNAL OF SYSTEMATICBACTERIOLOGY, Apr. 1993, p. 358-362 Vol. 43, No. 2 0020-7713/93/020358-05$02.00/0 Copyright 0 1993, International Union of Microbiological Societies Vibrio mytili sp. nov., from Mussels MAR~A-JESUS PUJALTE,'" MARGARITA ORTIGOSA,~MARIA-CAMINO URDACI,~ ESPERANZA GARAY,l AND PATRICK A. D. GRIMONT2 Departamento de Microbiologia, Facultad de Ciencias Biologicas, Universitat de Valencia, Burjassot, 46100 Valencia, Spain, and Unit6 des Enterobacteries, U199, Institut National de la Sante et de la Recherche Mkdicale, Institut Pasteur, F75724 Paris Ceda 15, France2 Five strains isolated from mussels (harvested off the Atlantic Spanish coast in 1985 and 1986) were found to be phenotypically distinct from previously described Vibrio species, They showed 94 to 100% intragroup relatedness as determined by DNA-DNA hybridization (S1 nuclease method) but were found to be only 1 to 25% related to other Vibrio species. These strains have all of the properties that define the genus Vibrio and can be clearly differentiated from other species by their positive responses in tests for Thornley's arginine dihydrolase, gas production from glucose, growth in media containing 10% NaCl, and acid production from sucrose, L-arabinose, D-xylose, and D-cellobiose and their negative responses in tests for lysine decarboxylase, the Voges-Proskauer reaction, growth without NaCl and at 40°C, hydrolysis of gelatin, casein, starch, DNA, and alginate, and acid production from D-mannose. The G+C ratio of the DNA is 45 to 46 mol%. The name Vibriu mytili is proposed for the new species; strain 165 (= CECT 632) is the type strain.
    [Show full text]