Recombinant Human FGF10 Recombinant Human Protein Expressed in E

Catalog # Aliquot Size

F830-40N-25 25 µg F830-40N-100 100 µg

Recombinant Human FGF10 Recombinant human protein expressed in E. coli cells

Catalog # F830-40N Lot # G3211-30

Product Description Specific Activity

Recombinant Human FGF10 was expressed in E. coli cells. The protein accession number is O15520

Gene Aliases

FGF-10, FGFA, Keratinocyte growth factor-2 Recombinant Human Fibroblast Growth Factor 10 (FGF10)

Endotoxin Level

<1.0 EU/µg of recombinant protein as determined by the LAL method.

Formulation The bioactivity was determined in a NIH/3T3 cell proliferation Lyophilized from 0.2 µm filtered solution in PBS (pH 7.0) and 1mM assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.

DTT Purity

Reconstitution Protocol Recombinant Human FGF10 A quick spin of the vial followed by reconstitution in distilled water resolved on a 15% SDS_PAGE gel to a concentration not less than 0.1 mg/mL. This solution can then under reducing conditions and be diluted into other buffers. stained with Coomassie Blue G- 250 Storage and Stability Approx. MW 19.0 kDa Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Scientific Background Recombinant Human FGF10 FGF-10 is involved in the initial budding as well as the continuous Recombinant human protein expressed in E. coli cells outgrowth of vertebrate limbs. FGF-10 mRNA is expressed preferentially in neurons but not in glial cells and may have a Catalog Number F830-40N distinct role in the brain. Human FGF-10 is mitogenic for fetal rat Specific Lot Number G3211-30 keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant Purity 95% FGF-10 induces the proliferation of human urothelial cells in vitro Stability 6 mos. at –20oC from date of and induces the proliferation of transitional epithelium. FGF-10 is shipment secreted by cultured mouse pre- adipocytes, prevention of FGF- Storage & Shipping Storage The lyophilized protein is 10 signaling inhibits subsequent differentiation. The ability of stable for at least one year from date of receipt at -70°C. Upon embryonic fibroblasts derived from FGF-10 knock-out mice to reconstitution, this cytokine can be differentiate into adipocytes is also impaired. stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.