Reptilian Skin As a Biomimetic Analogue for Design of Deterministic Tribo-Surfaces H
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Reptile-Like Physiology in Early Jurassic Stem-Mammals
bioRxiv preprint doi: https://doi.org/10.1101/785360; this version posted October 10, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Title: Reptile-like physiology in Early Jurassic stem-mammals Authors: Elis Newham1*, Pamela G. Gill2,3*, Philippa Brewer3, Michael J. Benton2, Vincent Fernandez4,5, Neil J. Gostling6, David Haberthür7, Jukka Jernvall8, Tuomas Kankanpää9, Aki 5 Kallonen10, Charles Navarro2, Alexandra Pacureanu5, Berit Zeller-Plumhoff11, Kelly Richards12, Kate Robson-Brown13, Philipp Schneider14, Heikki Suhonen10, Paul Tafforeau5, Katherine Williams14, & Ian J. Corfe8*. Affiliations: 10 1School of Physiology, Pharmacology & Neuroscience, University of Bristol, Bristol, UK. 2School of Earth Sciences, University of Bristol, Bristol, UK. 3Earth Science Department, The Natural History Museum, London, UK. 4Core Research Laboratories, The Natural History Museum, London, UK. 5European Synchrotron Radiation Facility, Grenoble, France. 15 6School of Biological Sciences, University of Southampton, Southampton, UK. 7Institute of Anatomy, University of Bern, Bern, Switzerland. 8Institute of Biotechnology, University of Helsinki, Helsinki, Finland. 9Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland. 10Department of Physics, University of Helsinki, Helsinki, Finland. 20 11Helmholtz-Zentrum Geesthacht, Zentrum für Material-und Küstenforschung GmbH Germany. 12Oxford University Museum of Natural History, Oxford, OX1 3PW, UK. 1 bioRxiv preprint doi: https://doi.org/10.1101/785360; this version posted October 10, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 13Department of Anthropology and Archaeology, University of Bristol, Bristol, UK. 14Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK. -
Reptile Genomes Open the Frontier for Comparative Analysis of Amniote Development and Regeneration MARC TOLLIS, ELIZABETH D
Int. J. Dev. Biol. 58: 863-871 (2014) doi: 10.1387/ijdb.140316kk www.intjdevbiol.com Reptile genomes open the frontier for comparative analysis of amniote development and regeneration MARC TOLLIS, ELIZABETH D. HUTCHINS and KENRO KUSUMI* School of Life Sciences, Arizona State University, Tempe, AZ, USA ABSTRACT Developmental genetic studies of vertebrates have focused primarily on zebrafish, frog and mouse models, which have clear application to medicine and well-developed genomic resources. In contrast, reptiles represent the most diverse amniote group, but have only recently begun to gather the attention of genome sequencing efforts. Extant reptilian groups last shared a common ancestor ~280 million years ago and include lepidosaurs, turtles and crocodilians. This phylogenetic diversity is reflected in great morphological and behavioral diversity capturing the attention of biologists interested in mechanisms regulating developmental processes such as somitogenesis and spinal patterning, regeneration, the evolution of “snake-like” morphology, the formation of the unique turtle shell, and the convergent evolution of the four-chambered heart shared by mammals and archosaurs. The complete genome of the first non-avian reptile, the green anole lizard, was published in 2011 and has provided insights into the origin and evolution of amniotes. Since then, the genomes of multiple snakes, turtles, and crocodilians have also been completed. Here we will review the current diversity of available reptile genomes, with an emphasis on their evolutionary relationships, and will highlight how these genomes have and will continue to facilitate research in developmental and regenerative biology. KEY WORDS: reptile, genomics, gene expression, somitogenesis, regeneration Introduction 2013) in addition to 28 avian genomes (Table 1; Zhang et al., 2014). -
A Review of Vertebrate Track-Bearing Formations
5 Lockley, M.G. & Lucas, S.G., eds., 2014, Fossil footprints of western North America: NMMNHS Bulletin 62 A REVIEW OF VERTEBRATE TRACK-BEARING FORMATIONS FROM THE MESOZOIC AND EARLIEST CENOZOIC OF WESTERN CANADA WITH A DESCRIPTION OF A NEW THEROPOD ICHNOSPECIES AND REASSIGNMENT OF AN AVIAN ICHNOGENUS RICHARD T. MCCREA1, LISA G. BUCKLEY1, A. GUY PLINT2, PHILIP J. CURRIE3, JAMES W. HAGGART4, CHARLES W. HELM1 AND S. GEORGE PEMBERTON5 1Peace Region Palaeontology Research Centre; Box 1540; Tumbler Ridge, British Columbia; V0C 2W0; CANADA; 2Department of Earth Sciences; University of Western Ontario; London, Ontario; N6A 5B7; CANADA; 3Department of Biological Sciences; University of Alberta, Edmonton, Alberta; T6G 2E9; CANADA; 4Geological Survey of Canada; 1500-605 Robson Street; Vancouver, British Columbia; V6B 5J3; CANADA; 5Department of Earth and Atmospheric Sciences; University of Alberta; Edmonton, Alberta; T6G 2E3; CANADA Abstract—The past quarter century has seen a marked increase in the recognition of fossil vertebrate tracksites in western Canada. Most of these finds were made in Alberta and British Columbia, but the Yukon Territory can lay claim to at least one tracksite and probably has the potential to yield more sites. The record of dinosaur tracks with skin impressions has increased dramatically, and is now represented by specimens of ankylosaurs, large ornithopods, small theropods and tyrannosauroids. Notable new finds include the first record of sauropods in Canada, evidence of herding behavior in ankylosaurs and the first pterosaur tracks in Canada. First discoveries of track specimens from several formations in western Canada include the Mountain Park Member of the Gates Formation in Alberta, and the Boulder Creek, Goodrich, Kaskapau, Cardium and Marshybank formations in northeastern British Columbia. -
History and Function of Scale Microornamentation in Lacertid Lizards
JOURNALOFMORPHOLOGY252:145–169(2002) HistoryandFunctionofScaleMicroornamentation inLacertidLizards E.N.Arnold* NaturalHistoryMuseum,CromwellRoad,LondonSW75BD,UK ABSTRACTDifferencesinsurfacestructure(ober- mostfrequentlyinformsfromdryhabitatsorformsthat hautchen)ofbodyscalesoflacertidlizardsinvolvecell climbinvegetationawayfromtheground,situations size,shapeandsurfaceprofile,presenceorabsenceoffine wheredirtadhesionislessofaproblem.Microornamen- pitting,formofcellmargins,andtheoccurrenceoflongi- tationdifferencesinvolvingotherpartsofthebodyand tudinalridgesandpustularprojections.Phylogeneticin- othersquamategroupstendtocorroboratethisfunctional formationindicatesthattheprimitivepatterninvolved interpretation.Microornamentationfeaturescandevelop narrowstrap-shapedcells,withlowposteriorlyoverlap- onlineagesindifferentordersandappeartoactadditively pingedgesandrelativelysmoothsurfaces.Deviations inreducingshine.Insomecasesdifferentcombinations fromthisconditionproduceamoresculpturedsurfaceand maybeoptimalsolutionsinparticularenvironments,but havedevelopedmanytimes,althoughsubsequentovert lineageeffects,suchaslimitedreversibilityanddifferent reversalsareuncommon.Likevariationsinscaleshape, developmentalproclivities,mayalsobeimportantintheir differentpatternsofdorsalbodymicroornamentationap- peartoconferdifferentandconflictingperformancead- genesis.Thefinepitsoftenfoundoncellsurfacesare vantages.Theprimitivepatternmayreducefrictiondur- unconnectedwithshinereduction,astheyaresmaller inglocomotionandalsoenhancesdirtshedding,especially thanthewavelengthsofmostvisiblelight.J.Morphol. -
Chapter18 Reptilia.Pdf
Chapter 18: Amniote Origins & Reptilian Groups Phylum: Chordata Subphylum: Vertebrata Class Reptilia (~8000 spp.) Order Chelonia: turtles & tortoises Order Squamata: lizards & snakes Order Rhynchocephalia : tuatara Order Crocodilia: crocodiles & alligators Reptiles • Characteristics – amniotic egg • chorion - outermost membrane • allantois - surrounds waste cavity • amnion - encases embryo • yolk sac - surrounds yolk (food) – dry skin – thoracic breathing 1 Amniote Origins amphibians tied to water a) lack shelled eggs b) often have gill-breathing larvae 3 monophyletic assemblage called Amniota named after innermost of three extraembryonic membranes, amnion before the end of the Paleozoic amniotes truly terrestrial 1 developed an egg lungs 2 Amniotes led to the three vertebrate groups a) reptiles b) birds c) mammals Diversity 1. paraphyletic class Reptilia include the first truly terrestrial vertebrates 2. Age of Reptiles lasted over 165 million years & included dinosaurs 3. mass extinction at the end of Mesozoic; modern reptiles represent surviving lineages 4. Tuatara (living fossil), sole survivor of a group that otherwise disappeared 100 mya New Zealand broke off from Australia 100 mya burrowers, nocturnal, eat insects, millipedes, worms reasons for its survival?? 5. lizards & snakes radiated into diverse & abundant groups 6. 300-million-year-old history of reptile life on earth complicated by widespread convergent & parallel evolution among many lineages 2 Changes in Traditional Classification of Reptilian Groups 1. Cladistic methodology insists on hierarchical arrangement of monophyletic groups 2. disqualifies traditional class Reptilia as a valid taxon because not monophyletic 3. Class Reptilia excludes birds, which descend from most recent common ancestor of reptiles 4. makes class Reptilia a paraphyletic group because does not include all descendants & their most recent common ancestor 5. -
FGF Signalling Regulates Early Tail Denticle Formation in Sharks Rory L
Cooper et al. EvoDevo (2017) 8:8 DOI 10.1186/s13227-017-0071-0 EvoDevo RESEARCH Open Access Developing an ancient epithelial appendage: FGF signalling regulates early tail denticle formation in sharks Rory L. Cooper, Kyle J. Martin, Liam J. Rasch and Gareth J. Fraser* Abstract Background: Vertebrate epithelial appendages constitute a diverse group of organs that includes integumentary structures such as reptilian scales, avian feathers and mammalian hair. Recent studies have provided new evidence for the homology of integumentary organ development throughout amniotes, despite their disparate fnal morpholo- gies. These structures develop from conserved molecular signalling centres, known as epithelial placodes. It is not yet certain whether this homology extends beyond the integumentary organs of amniotes, as there is a lack of knowl- edge regarding their development in basal vertebrates. As the ancient sister lineage of bony vertebrates, extant chon- drichthyans are well suited to testing the phylogenetic depth of this homology. Elasmobranchs (sharks, skates and rays) possess hard, mineralised epithelial appendages called odontodes, which include teeth and dermal denticles (placoid scales). Odontodes constitute some of the oldest known vertebrate integumentary appendages, predating the origin of gnathostomes. Here, we used an emerging model shark (Scyliorhinus canicula) to test the hypothesis that denticles are homologous to other placode-derived amniote integumentary organs. To examine the conservation of putative gene regulatory network (GRN) member function, we undertook small molecule inhibition of fbroblast growth factor (FGF) signalling during caudal denticle formation. Results: We show that during early caudal denticle morphogenesis, the shark expresses homologues of conserved developmental gene families, known to comprise a core GRN for early placode morphogenesis in amniotes. -
Comparative Morphology of the Skin of Natrix Tessellata (Family: Colubridae) and Cerastes Vipera (Family: Viperidae) Author(S): Rasha E
Comparative Morphology of the Skin of Natrix tessellata (Family: Colubridae) and Cerastes vipera (Family: Viperidae) Author(s): Rasha E. Abo-Eleneen and Ahmed A. Allam Source: Zoological Science, 28(10):743-748. Published By: Zoological Society of Japan DOI: http://dx.doi.org/10.2108/zsj.28.743 URL: http://www.bioone.org/doi/full/10.2108/zsj.28.743 BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use. Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder. BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research. ZOOLOGICAL SCIENCE 28: 743–748 (2011) ¤ 2011 Zoological Society of Japan Comparative Morphology of the Skin of Natrix tessellata (Family: Colubridae) and Cerastes vipera (Family: Viperidae) Rasha E. Abo-Eleneen1 and Ahmed A. Allam1,2* 1Department of Zoology, Faculty of Science, Beni-suef University, Beni-Suef 65211, Egypt 2King Saud University, College of Science, Zoology Department, Riyadh 11345, Saudi Arabia We studied beneficial difference of the skin of two snakes. -
Reptile Scale Paradigm: Evo-Devo, Pattern Formation and Regeneration
Int. J. Dev. Biol. 53: 813-826 (2009) DEVELOPMENTALTHE INTERNATIONAL JOURNAL OF doi: 10.1387/ijdb.072556cc BIOLOGY www.intjdevbiol.com Reptile scale paradigm: Evo-Devo, pattern formation and regeneration CHENG CHANG1,2, PING WU1, RUTH E. BAKER3, PHILIP K. MAINI3,4, LORENZO ALIBARDI*,5 and CHENG-MING CHUONG*,1 1Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA, 2School of Life Science, Lanzhou University, Lanzhou, Gansu, China, 3Centre for Mathematical Biology, Mathematical Institute, University of Oxford, 4Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, UK and 5Dipartimento di Biologia Evoluzionistica Sperimentale, University of Bologna, Bologna, Italy ABSTRACT The purpose of this perspective is to highlight the merit of the reptile integument as an experimental model. Reptiles represent the first amniotes. From stem reptiles, extant reptiles, birds and mammals have evolved. Mammal hairs and feathers evolved from Therapsid and Sauropsid reptiles, respectively. The early reptilian integument had to adapt to the challenges of terrestrial life, developing a multi-layered stratum corneum capable of barrier function and ultraviolet protection. For better mechanical protection, diverse reptilian scale types have evolved. The evolution of endothermy has driven the convergent evolution of hair and feather follicles: both form multiple localized growth units with stem cells and transient amplifying cells protected in the proximal follicle. This topological arrangement allows them to elongate, molt and regenerate without structural constraints. Another unique feature of reptile skin is the exquisite arrangement of scales and pigment patterns, making them testable models for mechanisms of pattern formation. Since they face the constant threat of damage on land, different strategies were developed to accommodate skin homeostasis and regeneration. -
Identification and Comparative Analysis of the Epidermal
www.nature.com/scientificreports OPEN Identification and comparative analysis of the epidermal differentiation complex in snakes Received: 14 November 2016 Karin Brigit Holthaus1,2, Veronika Mlitz1, Bettina Strasser1, Erwin Tschachler1, Accepted: 22 February 2017 Lorenzo Alibardi2 & Leopold Eckhart1 Published: 27 March 2017 The epidermis of snakes efficiently protects against dehydration and mechanical stress. However, only few proteins of the epidermal barrier to the environment have so far been identified in snakes. Here, we determined the organization of the Epidermal Differentiation Complex (EDC), a cluster of genes encoding protein constituents of cornified epidermal structures, in snakes and compared it to the EDCs of other squamates and non-squamate reptiles. The EDC of snakes displays shared synteny with that of the green anole lizard, including the presence of a cluster of corneous beta-protein (CBP)/beta-keratin genes. We found that a unique CBP comprising 4 putative beta-sheets and multiple cysteine-rich EDC proteins are conserved in all snakes and other squamates investigated. Comparative genomics of squamates suggests that the evolution of snakes was associated with a gene duplication generating two isoforms of the S100 fused-type protein, scaffoldin, the origin of distinct snake-specific EDC genes, and the loss of other genes that were present in the EDC of the last common ancestor of snakes and lizards. Taken together, our results provide new insights into the evolution of the skin in squamates and a basis for the characterization of the molecular composition of the epidermis in snakes. Snakes are reptiles that have lost their limbs during evolution and developed a unique predatory lifestyle that involves the ability to swallow prey of a diameter larger than that of their own body1–3. -
Fiftee N Vertebrate Beginnings the Chordates
Hickman−Roberts−Larson: 15. Vertebrate Beginnings: Text © The McGraw−Hill Animal Diversity, Third The Chordates Companies, 2002 Edition 15 chapter •••••• fifteen Vertebrate Beginnings The Chordates It’s a Long Way from Amphioxus Along the more southern coasts of North America, half buried in sand on the seafloor,lives a small fishlike translucent animal quietly filtering organic particles from seawater.Inconspicuous, of no commercial value and largely unknown, this creature is nonetheless one of the famous animals of classical zoology.It is amphioxus, an animal that wonderfully exhibits the four distinctive hallmarks of the phylum Chordata—(1) dorsal, tubular nerve cord overlying (2) a supportive notochord, (3) pharyngeal slits for filter feeding, and (4) a postanal tail for propulsion—all wrapped up in one creature with textbook simplicity. Amphioxus is an animal that might have been designed by a zoologist for the classroom. During the nineteenth century,with inter- est in vertebrate ancestry running high, amphioxus was considered by many to resemble closely the direct ancestor of the vertebrates. Its exalted position was later acknowledged by Philip Pope in a poem sung to the tune of “Tipperary.”It ends with the refrain: It’s a long way from amphioxus It’s a long way to us, It’s a long way from amphioxus To the meanest human cuss. Well,it’s good-bye to fins and gill slits And it’s welcome lungs and hair, It’s a long, long way from amphioxus But we all came from there. But amphioxus’place in the sun was not to endure.For one thing,amphioxus lacks one of the most important of vertebrate charac- teristics,a distinct head with special sense organs and the equipment for shifting to an active predatory mode of life. -
Development and Characterization of a Transdermal Formula for an Extract of the Medicinal Plant Harpagophytum Procumbens
Development and characterization of a transdermal formula for an extract of the medicinal plant Harpagophytum procumbens by Naushaad Ebrahim (B.Pharm.; M.Pharm.) submitted in the fulfilment for the requirements for the degree Philosophiae Doctor in the Faculty of Natural Sciences, School of Pharmacy (Pharmaceutics) at the University of the Western Cape. Supervisor: Prof. Reinhard Uebel Bellville, August, 2013 Table of Contents LIST OF FIGURES vi LIST OF TABLES xii ABBREVIATIONS xiv ABSTRACT xv ACKNOWLEDGEMENTS xix THESIS OVERVIEW xx CHAPTER ONE: Introduction and problem statement. 1 1.1 Introduction 1 1.2 Aim and objectives 5 CHAPTER TWO: Plant Medicine 7 2.1 Introduction to plant medicine 7 2.2.1 Synergy 10 2.2.2 Polyvalence 12 2.3 Formulation of plant extracts into conventional dosage forms. 13 2.4 Current therapy for inflammatory conditions. 14 2.4.1 Non-steroidal anti-inflammatory drugs (NSAIDs) 14 2.4.2 Selective COX-2 inhibitors 14 2.2.3 Iridiodes 15 2.5 Harpagophytum procumbens (Devil’s Claw) 15 2.5.1 Macroscopic description 16 2.5.2 Uses 16 2.5.3 Chemical composition 16 2.5.4 Harpagoside 17 2.5.5 Harpagide 17 2.5.6 Preparations 18 2.5.7 Clinical studies 18 2.5.8 Animal studies 19 2.5.9 In Vitro studies 19 2.5.9.1 Cyclooxygenase 2 19 2.5.9.2 DuP-697 21 i CHAPTER THREE: Transdermal Drug Permeation 23 3.1 Introduction 23 3.2 The skin structure 25 3.2.1 Stratum corneum 25 3.2.2 Viable epidermis 25 3.2.3 Dermis and Hypodermis 26 3.2.4 Skin appendages 26 3.3 Percutaneous absorption 26 3.4 Routes of transdermal delivery 28 3.4.1 Transcellular -
Keratins in the Epidermis of Chelonians, Lepidosaurians, and Archosaurians 1N 2 LORENZO ALIBARDI and ROGER H
JOURNAL OF EXPERIMENTAL ZOOLOGY 293:27–38 (2002) Immunocytochemical Analysis of Beta (b)Keratins in the Epidermis of Chelonians, Lepidosaurians, and Archosaurians 1n 2 LORENZO ALIBARDI AND ROGER H. SAWYER 1Dipartimento di Biologia evoluzionistica sperimentale, University of Bologna, 40126, Bologna, Italy 2 Biological Sciences Department, University of South Carolina, Columbia, South Carolina 29208 ABSTRACT Beta (b) keratins are present only in the avian and reptilian epidermises. Although much is known about the biochemistry and molecular biology of the b keratins in birds, little is known for reptiles. In this study we have examined the distribution of b keratins in the adult epidermis of turtle, lizard, snake, tuatara, and alligator using light and electron immunocytochemistry with a well-characterized antiserum (anti-b1 antiserum) made against a known avian scale type b keratin. In lizard, snake, and tuatara epidermis this antiserum reacts strongly with the beta-layer, more weakly with the oberhautchen before it merges with the beta-layer, and least intensely with the mesos layer. In addition, the anti-b1 antiserum reacts speci¢cally with the setae of climbing pads in gekos, the plastron and carapace of turtles, and the stratum corneum of alligator epidermis. Electron microscopic studies con¢rm that the reaction of the anti-b1 antiserum is exclusively with characteristic bundles of the 3-nm b keratin ¢laments in the cells of the forming beta-layer, and with the densely packed electron-lucent areas of b keratin in the mature bet- layer.These immunocytochemical results suggest that the 3-nm b keratin ¢laments of the reptilian integument are phylogenetically related to those found in avian epidermal appendages.