Rangelands of Central Asia: Proceedings of the Conference on Transformations, Issues, and Future Challenges
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Discrimination of Three Ephedra Species and Their Geographical
www.nature.com/scientificreports OPEN Discrimination of three Ephedra species and their geographical origins based on multi-element Received: 6 December 2017 Accepted: 22 June 2018 fngerprinting by inductively Published: xx xx xxxx coupled plasma mass spectrometry Xiaofang Ma1, Lingling Fan1, Fuying Mao1,2, Yunsheng Zhao1,2,3, Yonggang Yan4, Hongling Tian5, Rui Xu1, Yanqun Peng1 & Hong Sui1,2 Discrimination of species and geographical origins of traditional Chinese medicine (TCM) is essential to prevent adulteration and inferior problems. We studied Ephedra sinica Stapf, Ephedra intermedia Schrenk et C.A.Mey. and Ephedra przewalskii Bge. to investigate the relationship between inorganic element content and these three species and their geographical origins. 38 elemental fngerprints from six major Ephedra-producing regions, namely, Inner Mongolia, Ningxia, Gansu, Shanxi, Shaanxi, and Sinkiang, were determined to evaluate the importance of inorganic elements to three species and their geographical origins. The contents of 15 elements, namely, N, P, K, S, Ca, Mg, Fe, Mn, Na, Cl, Sr, Cu, Zn, B, and Mo, of Ephedra samples were measured using inductively coupled plasma mass spectroscopy. Elemental contents were used as chemical indicators to classify species and origins of Ephedra samples using a radar plot and multivariate data analysis, including hierarchical cluster analysis (HCA), principal component analysis (PCA), and discriminant analysis (DA). Ephedra samples from diferent species and geographical origins could be diferentiated. This study showed that inorganic elemental fngerprint combined with multivariate statistical analysis is a promising tool for distinguishing three Ephedra species and their geographical origins, and this strategy might be an efective method for authenticity discrimination of TCM. -
Diet of Gazella Subgutturosa (G黮denstaedt, 1780) and Food
Folia Zool. – 61 (1): 54–60 (2012) Diet of Gazella subgutturosa (Güldenstaedt, 1780) and food overlap with domestic sheep in Xinjiang, China Wenxuan XU1,2, Canjun XIA1,2, Jie LIN1,2, Weikang YANG1*, David A. BLANK1, Jianfang QIAO1 and Wei LIU3 1 Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, China; e-mail: [email protected] 2 Graduate School of Chinese Academy of Sciences, Beijing 100039, China 3 School of Life Sciences, Sichuan University, Chengdu 610064, China Received 16 May 2011; Accepted 12 August 2011 Abstract. The natural diet of goitred gazelle (Gazella subgutturosa) was studied over the period of a year in northern Xinjiang, China using microhistological analysis. The winter food habits of the goitred gazelle and domestic sheep were also compared. The microhistological analysis method demonstrated that gazelle ate 47 species of plants during the year. Chenopodiaceae and Poaceae were major foods, and ephemeral plants were used mostly during spring. Stipa glareosa was a major food item of gazelle throughout the year, Ceratoides latens was mainly used in spring and summer, whereas in autumn and winter, gazelles consumed a large amount of Haloxylon ammodendron. Because of the extremely warm and dry weather during summer and autumn, succulent plants like Allium polyrhizum, Zygophyllum rosovii, Salsola subcrassa were favored by gazelles. In winter, goitred gazelle and domestic sheep in Kalamaili reserve had strong food competition; with an overlap in diet of 0.77. The number of sheep in the reserve should be reduced to lessen the pressure of competition. -
Sequence Analysis of Chloroplast Chlb Gene of Medicinal Ephedra Species and Its Application to Authentication of Ephedra Herb
June 2006 Biol. Pharm. Bull. 29(6) 1207—1211 (2006) 1207 Sequence Analysis of Chloroplast chlB Gene of Medicinal Ephedra Species and Its Application to Authentication of Ephedra Herb a a b b b Yahong GUO, Ayako TSURUGA, Shigeharu YAMAGUCHI, Koji OBA, Kasumi IWAI, c,1) ,a Setsuko SEKITA and Hajime MIZUKAMI* a Graduate School of Pharmaceutical Sciences, Nagoya City University; 3–1 Tanabe-dori, Mizuho-ku, Nagoya 467–8603, Japan: b Research and Development Department, Asgen Pharmaceutical Co., Ltd.; 2–28–8 Izumi, Higashi-ku, Nagoya 461–8531, Japan: and c Tsukuba Medicinal Plant Research Station, National Institute of Health Sciences; 1 Hachimandai, Tsukuba, Ibaraki 305–0843, Japan. Received December 26, 2005; accepted February 15, 2006 Chloroplast chlB gene encoding subunit B of light-independent protochlorophyllide reductase was amplified from herbarium and crude drug specimens of Ephedra sinica, E. intermedia, E. equisetina, and E. przewalskii. Se- quence comparison of the chlB gene indicated that all the E. sinica specimens have the same sequence type (Type S) distinctive from other species, while there are two sequence types (Type E1 and Type E2) in E. equisetina. E. intermedia and E. prezewalskii revealed an identical sequence type (Type IP). E. sinica was also identified by di- gesting the chlB fragment with Bcl I. A novel method for DNA authentication of Ephedra Herb based on the se- quences of the chloroplast chlB gene and internal transcribed spacer of nuclear rRNA genes was developed and successfully applied for identification of the crude drugs obtained in the Chinese market. Key words chloroplast chlB; DNA authentication; Ephedra Herb; polymerase chain reaction-restriction fragment length poly- morphism Ephedra Herb is an important crude drug which has been nucleotide deletions were present in the trnL/trnF spacer of used in Chinese and Japanese traditional (Kampo) medi- E. -
Interdisciplinary Investigation on Ancient Ephedra Twigs from Gumugou Cemetery (3800B.P.) in Xinjiang Region, Northwest China
MICROSCOPY RESEARCH AND TECHNIQUE 00:00–00 (2013) Interdisciplinary Investigation on Ancient Ephedra Twigs From Gumugou Cemetery (3800b.p.) in Xinjiang Region, Northwest China 1,2 1,2 3 1,2 MINGSI XIE, YIMIN YANG, * BINGHUA WANG, AND CHANGSUI WANG 1Laboratory of Human Evolution, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing, 100044, China 2Department of Scientific History and Archaeometry, University of Chinese Academy of Sciences, Beijing 100049, China 3School of Chinese Classics, Renmin University of China, Beijing 100872, China KEY WORDS Ephedra; SEM; chemical analysis; GC-MS ABSTRACT In the dry northern temperate regions of the northern hemisphere, the genus Ephedra comprises a series of native shrub species with a cumulative application history reach- ing back well over 2,000 years for the treatment of asthma, cold, fever, as well as many respira- tory system diseases, especially in China. There are ethnological and philological evidences of Ephedra worship and utilization in many Eurasia Steppe cultures. However, no scientifically verifiable, ancient physical proof has yet been provided for any species in this genus. This study reports the palaeobotanical finding of Ephedra twigs discovered from burials of the Gumugou archaeological site, and ancient community graveyard, dated around 3800 BP, in Lop Nor region of northwestern China. The macro-remains were first examined by scanning electron microscope (SEM) and then by gas chromatography-mass spectrometry (GC-MS) for traits of residual bio- markers under the reference of modern Ephedra samples. The GC-MS result of chemical analy- sis presents the existence of Ephedra-featured compounds, several of which, including benzaldehyde, tetramethyl-pyrazine, and phenmetrazine, are found in the chromatograph of both the ancient and modern sample. -
1. EPHEDRA Linnaeus, Sp. Pl. 2: 1040. 1753. 麻黄属 Ma Huang Shu Morphological Characters and Geographical Distribution Are the Same As Those of the Family
Flora of China 4: 97–101. 1999. 1 1. EPHEDRA Linnaeus, Sp. Pl. 2: 1040. 1753. 麻黄属 ma huang shu Morphological characters and geographical distribution are the same as those of the family. 1a. Bracts of seed cones almost completely free, connate only at base, light brown and membranous at maturity ......................................................................................................................................... 1. E. przewalskii 1b. Bracts of seed cones usually connate for 1/3–5/6 their length, red and fleshy at maturity. 2a. Seeds prominently longitudinally ridged, with dense, tiny projections .............................. 3. E. rhytidosperma 2b. Seeds smooth, rarely finely longitudinally striate. 3a. Integument tube 3–5 mm, usually spirally twisted ............................................................. 2. E. intermedia 3b. Integument tube 1–2(–2.5) mm, straight, curved, or slightly twisted. 4a. Shrubs or subshrubs, usually 50–150 cm. 5a. Bracts of seed cones with margin broad, membranous, often erose; integument tube ca. 1.5 mm, slightly spirally twisted; seeds 2 or 3; subshrubs usually to 50 cm .......... 4. E. lomatolepis 5b. Bracts of seed cones with margin narrower, entire or almost so; integument tube 1–2 mm, straight or slightly curved; seeds 1 or 2; shrubs or subshrubs often more than 50 cm. 6a. Apical pair of bracts of seed cones connate for 3/4–8/9 their length; seeds finely striate dorsally ................................................................................................. 9. E. likiangensis 6b. Apical pair of bracts of seed cones connate for 1/2–2/3 their length; seeds completely smooth. 7a. Herbaceous branches virgate, often pruinose, 1–1.5 mm in diam., rigid; integument tube to 2 mm, straight or slightly curved; plants to 100 cm or more ................. -
Arid Vegetation Species in Xinjiang in Northwest China
Arid vegetation species in Xinjiang in Northwest China Yang Meilin Department of Management of Ecosystems and Environmental Changes in Arid Lands Xinjiang Institute of Ecology and Geography, CAS 12th of December 2015, Munich There are more than 1160 arid vegetation species in China. Turpan Eremophytes Botanical Garden(TEBG) has collected more than 700 arid vegetation species now, occupy 60 % arid vegetation of China. TEBG is called "Bank of desert plant". TEBG belongs to Xinjiang Institute of Ecology and Geography, CAS. TEBG joined to Botanic Gardens Conservation International(BGCI) in 1994. TEBG is not only a 3A scenic spot but also a popular science educational bases. Turpan Eremophytes Botanical Garden TEBG was built in 1976. TEBG is the largest eremophytes botanical garden in China,the area is 150ha. TEBG is the lowest eremophytes botanical garden in the world. Location Turpan Eremophytes Botanical Garden(TEBG) in Turpan of eastern Xinjiang in China (42°51’52.5”N, 89°11’06.8”E; 75-80 m below sea level. The climate is characterized by low rainfall, high evaporation, high temperatures and dry winds. Annual Minimum Maximum Annual Annual mean temperature temperaturemean mean temperature precipitation evaporation 13.9°C -28.0°C 47.6°C 16.4mm 3000mm Introduce There are 9 Specific Categorized Plants Gardens in TEBG. National Medicinal Botanical Garden Ornamental Garden Rare & Endangered Species Botany Saline Desert Botany Tamaricaceae Calligonum L. Energy Plant Area Desert Economic Plant Arid Land Vineyard National Medicinal Botanical Garden National Medicinal Botanical Garden was built in 1992, and covers an area of 0.5ha It has collected more than 60 medicinal vegetation species The species contain Uygur herbs, Kazakhstan herbs, Mongolia herbs and so on Capparis spinosa L. -
Secondary Metabolites of the Choosen Genus Iris Species
ACTA UNIVERSITATIS AGRICULTURAE ET SILVICULTURAE MENDELIANAE BRUNENSIS Volume LX 32 Number 8, 2012 SECONDARY METABOLITES OF THE CHOOSEN GENUS IRIS SPECIES P. Kaššák Received: September 13,2012 Abstract KAŠŠÁK, P.: Secondary metabolites of the choosen genus iris species. Acta univ. agric. et silvic. Mendel. Brun., 2012, LX, No. 8, pp. 269–280 Genus Iris contains more than 260 species which are mostly distributed across the North Hemisphere. Irises are mainly used as the ornamental plants, due to their colourful fl owers, or in the perfume industry, due to their violet like fragrance, but lot of iris species were also used in many part of the worlds as medicinal plants for healing of a wide spectre of diseases. Nowadays the botanical and biochemical research bring new knowledge about chemical compounds in roots, leaves and fl owers of the iris species, about their chemical content and possible medicinal usage. Due to this researches are Irises plants rich in content of the secondary metabolites. The most common secondary metabolites are fl avonoids and isofl avonoids. The second most common group of secondary metabolites are fl avones, quinones and xanthones. This review brings together results of the iris research in last few decades, putting together the information about the secondary metabolites research and chemical content of iris plants. Some clinical studies show positive results in usage of the chemical compounds obtained from various iris species in the treatment of cancer, or against the bacterial and viral infections. genus iris, secondary metabolites, fl avonoids, isofl avonoids, fl avones, medicinal plants, chemical compounds The genus Iris L. -
Ephedra: Asking for Trouble?
Ephedra: Asking For Trouble? By Scot Peterson A member of the phylum Gnetophyta, the Ephedra genus is a perennial, dioecious shrub that reaches 1 1/2 to 4 feet tall (7). There are multiple species of this genus that inhabit the desert regions in certain parts of the world. The three species E. sinica, E. intermedia, and E. equisetina are found in Asia, particularly China and Mongolia. Ephedra distacha is from Europe. India and Pakistan are home to E. gerardiana. North American species consist of E. nevadensis (Mormon tea), E. viridis (desert tea), E. americana, and E. trifurca (7). It takes an average of four years for the shrub to achieve maturation (10) and is harvested in the fall (11). Ephedra has been used medicinally for hundreds, even thousands of years in the regions where it grows. For more than 5000 years, Ephedra's stems have been dried to cure multiple ailments in China. The first records of its use can be found in a Chinese compilation of herbs called Shen Nong Ben Cao Jing (11), which dates back to the first century A.D. (5) E. sinica, called Tsaopen-Ma Huang (2), is the most common species used. Ma Huang refers to the stem and branch, whereas Ma Huanggen refers to the root and rhizome. Ma Huang was used primarily in the treatment of the common cold, asthma, hay fever, bronchitis, edema, arthritis, fever, hypotension, and urticaria (hives). Ma Huanggen's effect is believed to oppose that of the stem and branches. Its use was limited to the treatment of profuse night sweating" (7). -
Yunatov's Records of Wild Edible Plant Used By
Yunatov’s Records of Wild Edible Plant Used by the Mongols in Mongolia During 1940- 1951: Ethnobotanical Arrangements and Discussions YanYing Zhang Inner Mongolia Normal University https://orcid.org/0000-0003-4560-6930 Wurhan Wurhan Inner Mongolia Normal University Sachula Sachula Inner Mongolia Normal University Khasbagan Khasbagan ( [email protected] ) Inner Mongolia Normal University https://orcid.org/0000-0002-9236-317X Research Keywords: Yunatov, the Mongols in Mongolia, Wild Edible Plants, Ethnobotany Posted Date: September 14th, 2020 DOI: https://doi.org/10.21203/rs.3.rs-69220/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/24 Abstract Background: Researchers have rarely studied traditional botanical knowledge in Mongolia over the past 60 years, and existing studies had been based on the theory and methodology of ethnobotany. However, Russian scientists who studied plants in Mongolia in the 1940s and 1950s collected valuable historical records of indigenous knowledge and information on Mongolian herdsmen utilizing local wild plants. One of the most comprehensive works is titled: "Forage plants on grazing land and mowing grassland in the People's Republic of Mongolia" (FPM) by A. A. Yunatov (1909-1967). Yunatov’s work focused on forage plants in Mongolia from 1940 to 1951, which was published in 1954 as his early research. Later, the original FPM was translated into Chinese and Cyrillic Mongolian in 1958 and 1968, respectively. Materials: In addition to morphological characteristics, distribution, habitat, phenology, palatability and nutrition of forage plants, Yunatov recorded the local names, the folk understanding and evaluation of the forage value, as well as other relevant cultural meanings and the use of local wild plants in FPM through interviews. -
Tulip Meadows of Kazakhstan & the Tien Shan Mountains
Tulip Meadows of Kazakhstan & the Tien Shan Mountains Naturetrek Tour Report 14 - 29 April 2018 Hulthemia persica (national flower of Kaz) Tulipa greig ii Allium karataviense Iris orchioides Report and images by Neil Anderson Naturetrek Mingledown Barn Wolf's Lane Chawton Alton Hampshire GU34 3HJ UK T: +44 (0)1962 733051 E: [email protected] W: www.naturetrek.co.uk Tour Report Tulip Meadows of Kazakhstan & the Tien Shan Mountains Tour participants: Neil Anderson (leader) and Vladimir Epiketov (local botanist) Amina Farakhov (interpreter) with nine Naturetrek clients. Day 1 Saturday 14th April Six group members met up with Neil at Gatwick airport for a mid-morning flight to Istanbul, where we had a five-hour wait before our connecting flight to Almaty. Day 2 Sunday 15th April Lake Kapchagai After our night flight to Almaty, we transferred to the Kazzhol Hotel around 8am and headed into the dining area for a sumptuous breakfast. Here we met the other three group members, Steve, Rami and Katie, who had arrived the previous day. After getting changed we headed off to Lake Kapchagai, some 60+ kilometres north of Almaty, and the largest reservoir in the country. As we were driving in we could see our first tulips. These were the yellow Tulipa kolpakowskiana , a species we saw quite frequently over the next couple of days. We soon encountered another yellow flowered species Tulipa behmiana, which we found only here. Also providing some colour was the attractive blue Iris tenuifolia, a plant of the sands. The small creamy flowers of the small Tulipa busheana, were now well past their best. -
N. S. Volume LVIII ANNALI DI BOTANICA 2000 at This Meeting The
n. s. Volume LVIII ANNALI DI BOTANICA 2000 A PRELIMINARY LIST OF RARE OR ENDANGERED IRIDACEAE SPECIES At this meeting the importance of focussing on rare and endangered Iridaceae species in each country became evident. A complete check list of the conservation status of rare and endangered Irid a cea e species is not yet estabilished. Here we present (Appendix) the list of the World Conservation Monitoring Centre (WCMC)* for rare and endangered Iridaceae, according to the IUCN categories. Some problems occur in listing rare and endangered species. For example, although in Israel legislation has protected all Iris species since 1964, so that we have now a complete list, this is not the case in other countries and for other genera. Furthermore, unsolved taxonomic problems confused the issue in some species and the exact distribution of others is still unknown. Here, we want to stimulate colleagues working on Irid a c e a e to send new information to the monitoring centre to improve the current red list. In Italy, we have species of bearded irises which are endangered because of animal and/or human influence. For example, in some areas of Italy, wild boar populations have been promoted to favour hunting, with resulting damage to Iris populations which have been rapidly reduced to a few individuals, as I. relicta Colas, on Monte delle Fate (Southern Lazio), because the boars dig up Iris rhizomes. In other areas, the intensive construction of houses has endangered some populations, for example, in the case of/, setina Colas. (Latina), a bearded Iris that flowers in February. -
Identification of Two Cold Water-Soluble Polysaccharides from the Stems of Ephedra Sinica Stapf
Chinese Medicine, 2010, 1, 63-68 doi:10.4236/cm.2010.13013 Published Online December 2010 (http://www.SciRP.org/journal/cm) Identification of Two Cold Water-Soluble Polysaccharides from the Stems of Ephedra sinica Stapf Yonggang Xia, Jun Liang, Bingyou Yang, Qiuhong Wang, Haixue Kuang* Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China Email: [email protected] Received December 4, 2010; revised December 8, 2010; accepted December 10, 2010 Abstract Two polysaccharides (ESP-A1 and ESP-A2) were isolated from the cold water extract of Ephedra sinica Stapf and purified through ethanol precipitation, deproteinization and by ion exchange and gel-filtration chromatography. Their molecular weight was determined using high performance size exclusion chromatog- raphy and evaporative light scattering detector (HPSEC-ELSD) and their monosaccharide composition was analyzed by high performance capillary electrophoresis (HPCE) based on pre-column derivatization with 1- phenyl-3-methyl-5-pyrazolone (PMP). It was shown that ESP-A1 consisted of xylose, arabinose, glucose, mannose and galactose and ESP-A2 consisted of xylose, arabinose, rhamnose and galactose, in a molar ratio (%) of 3.2: 61.1: 11.1: 12.9: 11.6 and 20.6: 67.7: 5.0: 6.7, respectively. The molecular weights (Mw) of ESP-A1 and ESP-A2 were 5.83 × 104 Da and more than 200 × 104 Da, respectively. To the best of our knowledge, two neutral polysaccharides are now being reported for the first time in this study. Keywords: Ephedra sinica Stapf, Polysaccharides, Isolation and Purification, Molecular Weight, Monosaccharide Composition 1. Introduction monosaccharide composition so as to further investigate its mechanism of action.