DOCSLIB.ORG

Glucose : (Every Cell)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Glucose : (Every Cell) المقرر الكود المستوى المحاضرات نوع المادة العلمية بيانات التواصل من 6 إلى آخر أساسيات وأيض 203 ك ح الثاني محاضرة بالفصل PDF 01003899211 الكربوهيدرات الدراسي الثاني Course of General Metabolism 1. Lipids Metabolism (finished) 2. Carbohydrates Metabolism 3. Proteins Metabolism Metabolism In living organisms Microorganisms Plans Animals Human We have learned metabolism from other organisms Biological process in living organisms Digestion Absorption Excretion Metabolism Metabolism The Greek metabole, meaning change. It is the totality of an organism's chemical processes to maintain life. Anabolism Catabolism Amphibolic Building compounds Oxidative process Links between the need energy release free energy other two pathways (~P) e.g. Protein synthesis e.g. Krebs Cycle e.g. Glycolysis Biomedical Importance of metabolism Normal Metabolism Abnormal Metabolism A knowledge of It results from: ●- Nutritional deficiency metabolism in normal ●- Enzyme deficiency animal is a prerequisite to ●- Abnormal secretion of Hormones understand abnormal ●- Genetic diseases state underlying many e.g. diabetes mellitus diseases. It includes the variations and adaptations in metabolism due to periods of Starvation, Exercise, Pregnancy, and Lactation. Carbohydrates In human body 1. Glucose : (Every cell). 2. Galactose : (Lactose of milk & Galactolipids ). 3. Fructose : (Liver & Seminal fluid). 4. Lactose : (Blood & Milk of lactating female). 5. Glycogen : (Liver & Muscles). 6. Ribose and Deoxy-ribose : (RNA & DNA). 7. Glycoproteins and mucoproteins: (Prothrombin & Mucin in saliva). 8. Mucopolysaccharides : (as heparin and hyaluronic acid in connective tissues & as agglutinogens on RBCs giving what are called blood group antigens). 9. Others: (Vitamin C, D-Glucuronic acid, L-Ascorbic acid etc). Digestion of Carbohydrates ▲- A family of glycosidases degrade carbohydrates into monohexoses via catalyzing the hydrolysis of glycosidic bonds. ▲-These enzymes are usually specific to the type of bond to be broken. ▲-Each enzyme has its optimum pH. ▲-In the mouse, the digestion of complex carbohydrates starts by the action of amylase enzyme (pH= 6.8) 1. Carbohydrate Metabolism ▲-After intake and absorption of glucose (e.g. after eating or drinking ), liver condenses extra glucose to glycogen. ▲-Then: ●1/3 of the body’s glycogen is stored in liver ● it is released as glucose to bloodstream ● If blood glucose falls ( e.g. during fasting) ● liver hydrolyzes glycogen to glucose Glycogen is bulky, so, we store only so much for short term energy supply ▲-You must know that:Fats are the long term energy supply Carbohydrate Metabolism (Continued) Metabolism The chemical needed changes that take place in a cell that produce energy and basic materials for important life processes -Millions of cells are included -Multiple organs (liver, adipose, heart, brain) are involved during such process -Thousands of enzymes are used during the metabolic -Various conditions (fed, fasted, exercise, stress) 1. Digestion & Absorption of Carbohydrates. 2. Glucose Oxidation: i. Major Pathways: (Mitochondrial or aerobic pathway, TCA cycle or Krebs cycle). ii. Minor pathways: (Pentose phosphate pathway + Uronic acid pathway) 3. Metabolism of Fructose, Galactose & Mannose. 4. Metabolism of Glycogen (Glycogenolysis + Glycogenesis). 5. Gluconeogenesis. Absorption of Carbohydrates Target: Simple Sugars (Glucose, Fructose, Galactose) Tissue Cell The product of digestion provide the tissues with Hexoses: (Fuel which are used to power the living process within the cells). Digestion of carbohydrates A- Pre-stomach : Via Salivary amylase a 1-4 endoglycosidase: G G G G G a Limit dextrins G G G G G G G G G G G amylase G G G G G G G a 1-6 link G G maltotriose G G a 1-4 link G G G G G maltose G G isomaltose B-Small Intestine: • Pancreatic enzymes a-amylase maltotriose maltose G G G G G G G G + G G a amylase amylose G G G G G G G G G G G G G G G G G amylopectin a Limit dextrins In the Small intestine (continued): - Portal for transport of virtually all nutrients - Water and electrolyte balance Enzymes associated with intestinal Surface membranes i. Sucrase ii. a dextrinase iii.Glucoamylase (maltase) iv. Lactase v. peptidases Oligosaccharide digestion (cont.) G G G G c-In The Stomach • No much carbohydrate digestion (because of the high G G pH of the stomach that inhibits salivary amylase) sucrase G • Acid and pepsin to unfold proteins a Limit dextrins • Ruminants have fore stomachs with extensive microbial populations to breakdown and an aerobically ferment G G feed G G maltase Glucoamylase (maltase) or G G G a- dextrinase G G a- dextrinase G G G G G Maltase: Specifically removes a single glucose from the nonreducing end of a linear α1-4 glucose G G G chain…breaking down maltose into glucose. (exosaccharidases) G Alpha dextinase : Cleaves 1,6-alpha glucosidic linkages Glucose transporter Carbohydrate absorption (GLUT, 14 types) isoforms GLUT1, GLUT3 and GLUT4: Are primarily Involved in Glucose transporter glucose uptake from the blood -GLUT2: liver, kidney can either transport glucose to these cells (hyperglycemia) or from them into the blood ( fasting or hypoglycemia). -GLUT5: Transporter of fructose in the small intestine and tests. (PAGE 95) apical basolateral Defects of Carbohydrates Digestion & Absorption 1. Lactase deficiency: The presence of Lactose in the intestine causes an Increase in the osmotic pressure. So, water will be drawn from the tissues (causing Dehydration) into the large intestine, a process which causes Diarrhea. The increase in the fermentation of Lactose by bacteria lead to production of CO2 gas. This cause distention and abdominal cramps. Treatment: remove milk from diet. 2. Sucrase deficiency: Rare condition show the same symptoms of lactase deficiency. it occurs early in childhood. 3. Monosacchrides malabsorption: A defect in the carrier mechanism (congenital conditions) of glucose and galactose. Treatment: No treatment. Carbohydrate malabsorption ●Lactose intolerance (hypolactasia). ● Decline lactase with age ● Lactose fermented in LI – • Gas and volatile FA • Water retention • diarrhea/bloating ● Not all populations (incidence of the enzyme deficiency): • Northern European – low incidence • Asian/African Americans – High b 1-4 linkage Glycolysis Carbohydrates serve as primary source of energy in the cell and Central to all metabolic processes. Glucose Cytosol - anaerobic Hexokinase Pentose Phosphate Glucose-6-P Glc-1- phosphate Shunt Glycolysis glycogen Pyruvate 1. Digestion & Absorption of Carbohydrates. a. Glucose Oxidation: i. Major Pathways: (Mitochondrial or aerobic pathway, TCA cycle or Krebs cycle). ii. Minor pathways: (Pentose phosphate pathway + Uronic acid pathway) b. Metabolism of Fructose, Galactose & Mannose. c. Metabolism of Glycogen (Glycogenolysis + Glycogenesis). d. Gluconeogenesis. Glycolysis (continued) * Oxidation of glucose is known as Glycolysis. * Glucose is oxidized to either Pyruvate or Lactate. •Under aerobic conditions (i.e. when oxygen is present), the dominant glycolytic product in most tissues is Pyruvate and the pathway is known as Aerobic Glycolysis. •Under anaerobic conditions (i.e. when oxygen is depleted) as for instance during prolonged vigorous exercise, the dominant glycolytic product in many tissues is Lactate and the pathway is known as Anaerobic Glycolysis. Glycolysis Stage 1 Energy requiring (consuming) stage - ATP - ATP Glycolysis Stage 2 (Energy producing stage) 2 Molecules. + 2 ATP + 2 ATP Oxygen requiring step Pathway of Cytoplasmic Glycolysis of Glucose Special feature of Anaerobic Glycolysis in RBCs 1. RBCs contain no mitochondria. Therefore, they depend only on anaerobic glycolysis to produce 2 ATP. 2. The RBCs have the ability to form 2,3 Bisphospho- glycerate (2,3-DPG) through Rapoport- Luebering cycle using mutase enzyme. *. The presence of 2,3 DPG lowers the affinity of Hb to O2 leading to good oxygenation of tissues and formation of Rapoport-Luebering cycle pyruvate. Special Features of Anaerobic Glycolysis in RBCs Clinical significance of 2,3-DPG: 1. During storage of blood in blood banks, 2,3-DPG concentration decreases gradually to reach 0.5 mg after 10 days (normal: 4.5 mg). This lead to high O2 affinity of the stored blood which is not desirable for blood transfusion. *. As 2,3 DPG can not penetrate RBCs, addition of Inosine (a substance can penetrate erythrocytes and changed into 2,3-DPG through HMP pathway) is advisable. 2. Also, persons living at high altitudes undergo a state of low O2 affinity due to increase of 2,3-DPG. *. This disappears on returning to the Sea Level I.e. a state of normal oxygen affinity is obtained. 3. Moreover, fetal hemoglobin (HbF) binds less strongly with 2.3-DPG than adult hemoglobin (HbA) consequently it has a higher O2 affinity. This may be a source of pathogenesis in patients carrying such type of haemoglobin. Energy (ATP) Production of Cytoplasmic Glycolysis Generally, ATP Production = ATP Produced – ATP Utilized 1-Anaerobic Glycolysis: Anaerobic ATP production = 4 ATP – 2 ATP = 2 2-Aerobic Glycolysis (if oxygen get available) Aerobic aATP p)roduction == 4 + 6* (or 4 + 4*) ATP – 2 ATP = 8 (or 6) •From extra-mitochondrial oxidation of 2 NADH + H+ by respiratory chain phosphorylation in mitochondria using two special carriers for hydrogen. Carbohydrates • Serve as primary source of energy in the cell • Central to all metabolic processes Glucose Cytosol - anaerobic Hexokinase Pentose Phosphate Glucose-6-P Glc-1- phosphate Shunt glycolysis glycogen Pyruvate cytosol Pyruvate mitochondria
Load more

Recommended publications
  • 4202-B: Nucleic Acids and Carbohydrates L-4 1 Deoxy Sugars
    4202-B: Nucleic acids and Carbohydrates L-4 Deoxy sugars In these sugars one of the OH groups is replaced by a hydrogen. 2-Deoxyribose (oxygen missing at C-2 position) is an important example of a deoxy sugar. It is important component of DNA, and lack of C-2 hydroxyl provide additional stability to it as compared to RNA as no intramolecular nuclephilic attack on phosphate chain can occur. Amino sugars In amino sugars one of the OH groups is replaced by an amino group. These molecules allow proteins and sugars to combine and produce structures of remarkable variety and beauty. The most common amino sugars are N-acetyl glucosamine and N-acetyl galactosamine, which differ only in stereochemistry. The hard outer skeletons of insects and crustaceans contain chitin, a polymer very like cellulose but made of N-acetyl glucosamine instead of glucose itself. It coils up in a similar way and provides the toughness of crab shells and beetle cases. Some important antibiotics contain amino sugars. For example, the three subunits of the antibiotic gentamicin are deoxyamino sugars (the middle subunit is missing the ring oxygen). N-Acetyl glucosamine N-Acetyl galactosamine Gentamicin, an antibiotic Cell membranes must not be so impermeable as they need to allow the passage of water and complex molecules. These membranes contain glycoproteins—proteins with amino sugar residues attached to asparagine, serine, or threonine in the protein. The attachment is at the anomeric position so that these compounds are O- or N-glycosides of the amino sugars. The structure below shows N-acetyl galactosamine attached to an asparagine residue as an N-glycoside.
    [Show full text]
  • Identification of L-Iduronic Acid As a Constituent of the Major Extracellular Polysaccharide Produced by Butyriuibrio Fibrisoluens Strain X6C61
    FEMS Microbiology Letters 51 (1988) 1-6 Published by Elsevier FEM 03178 Identification of L-iduronic acid as a constituent of the major extracellular polysaccharide produced by Butyriuibrio fibrisoluens strain X6C61 Robert J. Stack, Ronald D. Plattner and Gregory L. Cote Northern Regional Research Cen:er,. Agricultural Research Service, u.s. Department ofAgriculture, 181:J N. University St., Peoria, 11., U.S.A. Received 8 February 1988 Accepted 12 February 1988 Key words: L-iduronic acid; Iduronolactone; Butyriuibrio fibrisolvens; Rumen; Extracellular polysaccharide 1. SUMMARY 2. INTRODUCTION Butyrivibrio fibrisolvens strain X6C61 produces Butyrivibrio fibrisolvens is one of the most fre­ two extracellular polysaccharides (EPS-I and EPS­ quently isolated species of ruminal bacteria [1,2]. II) separable by anion-exchange chromatography. There are, at present, a large number of isolates The neutral sugar constituents of EPS-I were iden­ that fit the species description, with correspond­ tified by gas-liquid chromatography (GLC) as the ingly wide range of reported metabolic activities alditol acetates of rhamnose, mannose, galactose, [3]. glucose, and an unidentified component. These Stack [4] has recently reported that many strains results were confirmed using thin-layer chro­ of B. fibrisolvens produce EPS containing unusual matography (TLC). Neutral sugar analysis of monosaccharide constituents. For example, B. EPS-II, which eluted from DEAE-Sephadex at 0.4 fibrisolvens strain CF3 produces an EPS which M NaCl, yielded the alditol acetates of rhamnose, contains L-altrose [5], the first reported occurrence galactose, glucose, and idose. However, idose was of this hexose in nature. However, analysis of not found when hydrolysates of EPS-II were L-altrose-containing EPS by conventional alditol analysed by TLC.
    [Show full text]
  • Oxidation of Uronic Acids by a Large Excess of Glucose Oxidase Preparations
    1 •k J. Appl. Glycosci., Vol.46, No.1, p.1-7 (1999)•l Oxidation of Uronic Acids by a Large Excess of Glucose Oxidase Preparations Mikihiko Kobayashi,* Hirofumi Nishihara1 and Shoichi Kobayashi National Food Research Institute (2-1-2, Kannondai, Tsukuba 305-8642, Japan)1 Department of Applied Bioresource Science, School of Agriculture, Ibaraki University (3998 Ami-machi, Ibaraki 300-0393, Japan) Three commercial enzyme preparations of glucose oxidase (GOD) showed an oxidizing ability of glucuronic acid and galacturonic acid to form sugar acids. The optimum conditions of GOD reaction were at pH 8.0 and 50•Ž, whereass those of uronic acid oxidase (UOD) reaction were at pH 3.5 and 40•Ž. In spite of extensive attempts to separate GOD from UOD, the isolation of each activity was unsuccessful, and UOD reaction resulted from the wide substrate specificity of GOD reaction. More than 6-fold higher Km values for glucuronic acid and galacturonic acid than for glucose might suggest that UOD reaction was the alternative reaction of GOD enzyme. However, a more definite conclusion on UOD activity should be drawn from further studies. The reaction products from uronic acids were analyzed by HPLC and paper chromatography, and some products were shown to be identical with sugar acids. Beet pulp contained a large amount of uronic oxidation of uronic acids with glucose oxidase acids, which were found not only in the pectic (GOD) preparation from commercial sources. fraction, but also in the hemicellulose and cellulose, fractions.1) Although the effective MATERIALS AND METHODS saccharification of beet pulp might increase biomass content about 1.4-fold for the ethanol Materials.
    [Show full text]
  • Structure and Bioactivity of a Polysaccharide Containing Uronic Acid
    Carbohydrate Polymers 152 (2016) 222–230 Contents lists available at ScienceDirect Carbohydrate Polymers j ournal homepage: www.elsevier.com/locate/carbpol Structure and bioactivity of a polysaccharide containing uronic acid from Polyporus umbellatus sclerotia a,b b,c,∗ c c,d b,∗ Pengfei He , Anqiang Zhang , Fuming Zhang , Robert J. Linhardt , Peilong Sun a College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China b College of Ocean, Zhejiang University of Technology, Hangzhou 310032, China c Departments of Chemistry and Chemical Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA d Department of Biological Science, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA a r t i c l e i n f o a b s t r a c t Article history: Polyporus umbellatus is a medicinal fungus, has been used in traditional Chinese medicine for thou- Received 22 April 2016 sands years for treatment of edema, scanty urine, vaginal discharge, jaundice and diarrhea. The structure Received in revised form 29 June 2016 of a soluble polysaccharide (named PUP80S1), purified from the sclerotia of Polyporus umbellatus was Accepted 3 July 2016 elucidated by gas chromatography (GC), GC–mass spectrometry and nuclear magnetic resonance spec- Available online 4 July 2016 troscopy. PUP80S1 is a branched polysaccharide containing approximately 8.5% uronic acid and having an average molecular weight of 8.8 kDa. Atomic force microscopy of PUP80S1 reveals a globular chain confor- Keywords: mation in water. Antioxidant tests, Oxygen radical absorption capacity and 2,2-diphenyl-1-picrylhydrazyl Polyporus umbellatus Polysaccharide radical scavenging assays indicate that PUP80S1 possesses significant antioxidant activity.
    [Show full text]
  • Carohydrate Metabolism Part 2 By
    CAROHYDRATE METABOLISM PART 2 BY PROF.DR. SOUAD ABOAZMA OXIDATION OF GLUCOSE The pathways for oxidation of glucose are classified into two main groups: a- The major pathways for complete oxidation of glucose into CO2, H2O and energy are: 1- Glycolysis → convert one molecule of glucose into 2 mol of pyruvic acid + 2 NADH.H+. 2- Oxidative decarboxylation of pyruvic to acetyl CoA + NADH.H++CO2 3- Complete oxidation of acetyl CoA in Kerb’s cycle into CO2, H2O and energy . b- The minor pathways for oxidation, which are not for energy production. 1- Hexose monophosphate pathway (HMP). 2- Uronic acid pathway. GLYCOLYSIS EMBDEN-MEYERHOF PATHWAY Def.: oxidation of glucose to give pyruvic acid in presence of O2 and lactic acid in absence of mitochondria (RBCs) and in absence of O2 . Site: Cytoplasm of all cells especially muscles and RBCs. Steps: H – C = O H – C = O H C – OH H – C – OH Hexokinase, glucokinase OH – C – H OH – C – H H – C – OH Mg H – C – OH H – C – OH ATP ADP H – C – OH CH2OH CH2O-P D-Glucose G-6-P Mechanism of oxidation of glyceraldehydes 3-phosphate. Enz: glyceraldehydes 3-P dehydrogenase which is inhibited by the –SH poison iodoacetate, thus able to inhibit glycolysis. ENERGY PRODUCTION FROM GLYCOLYSIS: A. glycolysis in presence of O2 (Aerobic glycolysis): Reaction catalyzed by ATP production Stage I 1. Hexokinase/Glucokinase reaction (for -1 ATP phosphorylation) 2. Phosphofrutokinase-1 (for phosphorylation) -1 ATP Stage III 3. Glyceraldehyde-3-P dehydrogenase (oxidation of + 6 or +4 ATP 2 NADH in electron transport chain) 4.
    [Show full text]
  • Differential Gas-Liquid Chromatography Method for Determination of Uronic Acids in Carbohydrate Mixtures
    ANALYTICAL BIOCHEMISTRY 115, 410-418 (1981) 4889 Differential Gas-Liquid Chromatography Method for Determination of Uronic Acids in Carbohydrate Mixtures JACOB LEHRFELD Northern Regional Research Center. Agricultural Research. Science and Education Administration. U. S. Department of Agriculture.' Peoria. Jllinois 61604 Received March 17, 1981 An integrated gas-liquid chromatography method is described for the quantitation of mix­ tures containing simple monosaccharides in addition to mannuronic, glucuronic, and/or ga­ lacturonic acids. A hydrolyzed sample is divided into two portions. One portion is analyzed by the standard aldononitrile method. Glucuronic, galacturonic, and mannuronic acids are converted into compounds that do not chromatograph in the region of the standard aldononitrile acetates. Thus, this analysis gives an accurate estimation of the neutral monosaccharide con­ tent. The second portion is analyzed by a modified alditol acetate procedure. The reduction step is repeated three times to convert mannuronic, galacturonic, and glucuronic acids to their corresponding alditols via their intermediate lactones. The results of this gas-liquid chro­ matography analysis reflect the sum of the monosaccharides present plus their corresponding uronic acids. The difference between the values obtained by the aldononitrile acetate method and the modified alditol acetate method, therefore, is a measure of the uronic acid(s) present. Heteropolysaccharides containing only heating time must be carefully controlled, neutral sugars are readily analyzed by the as well as the temperature of the reaction alditol acetate (1-4) or the aldononitrile ac­ when the sulfuric acid is added. The presence etate (5-9) procedures. However, the pres­ of other neutral sugars requires the addition ence of uronic acids complicates the analysis.
    [Show full text]
  • Lecture: 2 OCCURRENCE and STRUCTURE of MONOSACCHARIDES the Simplest Monosaccharide That Possesses a Hydroxyl Group and a Carbony
    Lecture: 2 OCCURRENCE AND STRUCTURE OF MONOSACCHARIDES The simplest monosaccharide that possesses a hydroxyl group and a carbonyl group with an asymmetric carbon atom is the aldotriose -glyceraldehyde. (A carbon is said to be asymmetric if four different groups or atoms are attached to it. The carbon is also called as a chiral center). Glyceraldehyde is considered as a reference compound and it exists in two optically active forms, D and L The two families of monosaccharides, D-and L occur based on the configuration of D and L glyceraldehydes. In general, the D-family of sugars occur in nature. For monosaccharides with two or more asymmetric carbons, the prefixes D or L refer to the configuration of the penultimate carbon (i.e, the asymmetric carbon farthest from the carbonyl carbon). If the hydroxyl group on the penultimate carbon is on the right-hand side of the carbon chain when the aldehyde or ketone group is written at the top of the formula it belongs to the D family and if on the left hand side it belongs to L family. The D or L has nothing to do with optical activity. D sugars may be dextro- or levorotatory. The important monosaccharides containing aldehyde group belonging to the D family are the aldotetrose - D-erythrose the aldopentoses - D-ribose, D-arabinose and D-xylose the aldohexoses - D-glucose, D-mannose and D-galactose . The important monosaccharide belonging to the L-family is L-arabinose. The important ketoses are Ketotriose - dihydroxy acetone (It is optically inactive since there is no asymmetric carbon); the ketotetrose - D-erythrulose; the ketopentoses - D-ribulose and D-xylulose the ketohexose - D-fructose Cyclic structure of Monosaccharides: The monosaccharides exist either in cyclic or acyclic form.
    [Show full text]
  • Peer-Review Article
    PEER-REVIEWED ARTICLE bioresources.com SIMULTANEOUS SEPARATION AND QUANTITATIVE DETERMINATION OF MONOSACCHARIDES, URONIC ACIDS, AND ALDONIC ACIDS BY HIGH PERFORMANCE ANION- EXCHANGE CHROMATOGRAPHY COUPLED WITH PULSED AMPEROMETRIC DETECTION IN CORN STOVER PREHYDROLYSATES Xing Wang,a,b Yong Xu,a,b,c,* Li Fan,a,b Qiang Yong,a,b and Shiyuan Yu a,b A method for simultaneous separation and quantitative determination of arabinose, galactose, glucose, xylose, xylonic acid, gluconic acid, galacturonic acid, and glucuronic acid was developed by using high performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). The separation was performed on a CarboPacTM PA-10 column (250 mm × 2 mm) with a various gradient elution of NaOH-NaOAc solution as the mobile phase. The calibration curves showed good linearity (R2 ≥ 0.9993) for the monosaccharides, uronic acids, and aldonic acids in the range of 0.1 to 12.5 mg/L. The detection limits (LODs) and the quantification limits (LOQs) were 4.91 to 18.75 μg/L and 16.36 to 62.50 μg/L, respectively. Relative standard deviations (RSDs) of the retention times and peak areas for the seven consecutive determinations of an unknown amount of mixture were 0.15% to 0.44% and 0.22% to 2.31%, respectively. The established method was used to separate and determine four monosaccharides, two uronic acids, and two aldonic acids in the prehydrolysate from dilute acid steam-exploded corn stover within 21 min. The spiked recoveries of monosaccharides, uronic acids, and aldonic acids ranged from 91.25% to 108.81%, with RSDs (n=3) of 0.04% ~ 6.07%.
    [Show full text]
  • Synthesis of Glycosaminoglycans by Undifferentiated and Differentiated HT29 Human Colonie Cancer Cells1
    [CANCER RESEARCH 47, 4478-4484, August 15, 1987] Synthesis of Glycosaminoglycans by Undifferentiated and Differentiated HT29 Human Colonie Cancer Cells1 Patricia Simon-Assmann,2 FrançoiseBouziges,3 Daniele Daviaud, Katy Haffen, and MichèleKedinger INSERM Unité61,Biologie Cellulaire et Physiopathologie Digestives, 3 avenue Molière,67200 Strasbourg Hautepierre, France ABSTRACT colon carcinoma cell line established by Fogh and Trempe (11). Indeed, as discovered by Zweibaum's group (12-14), this cell Among the extracellular matrix components which have been sug gested to be involved in developmental and neoplastic changes are gly- line displays typical enterocytic differentiation characteristics cosaminoglycans (GAGs). To try to correlate their amount and nature (apical brush borders, tight junctions, presence of hydrolases) with the process of enterocytic differentiation, we studied glycosamino- when cultured in the total absence of sugar, in contrast to cells glycan synthesis of human colonie adenocarcinoma cells (HT29 cell line) grown with glucose which always remain undifferentiated (14). by [3H]glucosamine and |35S]sulfate incorporation. Enterocytic differen The differentiation process starts after the cells have reached tiation of the cells obtained in a sugar-free medium (for review, see A. confluency and continues for at least 20 days (13). Zweibaum ft al. In: Handbook of Physiology. Intestinal Transport of the In the present study, sulfated and nonsulfated GAGs from Gastrointestinal System, in press, 1987) resulted in a marked increase in undifferentiated (HT29G+) and differentiated (HT29G-) cells total incorporation of labeled precursors (20-fold for |3H)glucosamine, 4.5-fold for |35S|sulfate) as well as in uronic acid content (5-fold); most were analyzed.
    [Show full text]
  • Production of Hexaric Acids from Biomass
    International Journal of Molecular Sciences Review Production of Hexaric Acids from Biomass Riku Sakuta and Nobuhumi Nakamura * Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan * Correspondence: [email protected] Received: 29 June 2019; Accepted: 24 July 2019; Published: 26 July 2019 Abstract: Sugar acids obtained by aldohexose oxidation of both the terminal aldehyde group and the hydroxy group at the other end to carboxyl groups are called hexaric acids (i.e., six-carbon aldaric acids). Because hexaric acids have four secondary hydroxy groups that are stereochemically diverse and two carboxyl groups, various applications of these acids have been studied. Conventionally, hexaric acids have been produced mainly by nitric acid oxidation of aldohexose, but full-scale commercialization has not been realized; there are many problems regarding yield, safety, environmental burden, etc. In recent years, therefore, improvements in hexaric acid production by nitric acid oxidation have been made, while new production methods, including biocatalytic methods, are actively being studied. In this paper, we summarize these production methods in addition to research on the application of hexaric acids. Keywords: aldaric acids; biorefinery; biofuel cell; bioprocess; biorefinery; carbohydrates; electrochemistry; green chemistry; oxidation; sustainable chemistry 1. Introduction The International Energy Agency defines a biorefinery as “the sustainable processing of biomass into a spectrum of marketable products and energy”, which is the most comprehensive and commonly accepted definition [1]. Because inexpensive petroleum-derived chemicals are already mass produced, the production of bio-based chemicals has been limited to those with structures that are too complex for the fine-chemicals market to justify their expensive production costs [2].
    [Show full text]
  • Correlation Between Structure and Function of Heparin (Mucopolysaccharide/Anticoagulant Function) ROBERT D
    Proc. Natl. Acad. Sci. USA Vol. 76, No. 3, pp. 1218-1222, March 1979 Biochemistry Correlation between structure and function of heparin (mucopolysaccharide/anticoagulant function) ROBERT D. ROSENBERG AND LUN LAM The Sidney Farber Cancer Institute, Beth Israel Hospital; and Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115 Communicated by K. Frank Austen, January 2, 1979 ABSTRACT We have fractionated crude porcine heparin glucuronide (Koch-Light Laboratories, Colnbrook, England) to obtain highly active as well as relatively inactive species of as substrate (5). The activity of a-iduronidase was measured molecular weight ;7000 with specific anticoagulant activities by a spectrophotometric technique utilizing phenyl iduronide of 360 and 12 units/mg, respectively. Nitrous acid degradation of both of these polymers yielded a tetrasaccharide fraction, 1B, (Calbiochem) as substrate (6). The level of iduronate sulfatase that contained equimolar amounts of iduronic and glucuronic activity was estimated by a high-voltage electrophoretic method acids, possessed an internal N-acetylated glucosamine, and using iduronsyl-2-sulfate-[H3]anhydromannitol-6-sulfate as carried anhydromannitol at the reducing end position. The 1:B substrate (7). tetrasaccharide derived from the highly active heparin, fla, The molecular weights of heparin samples were determined was recovered in a yield of 1.1 mol/7000 daltons. Our analyses on a Sephadex G-100 column (0.6 X 190 indicate that at least 95% of the lfSa is a single structure that by gel chromatography consists of the following unique monosaccharide sequence: L- cm) equilibrated with 0.5 M NaCl/0.01 M Tris-HCl, pH 7.5. iduronic acid - N-acetylated D-glucosamine-6sulate -D The column was calibrated with heparin standards whose glucuronic acid - N-sulfate D-glucosamine-6sulfate.
    [Show full text]
  • Selective Oxidation of Uronic Acids Into Aldaric Acids Over Gold Catalyst
    RSC Advances View Article Online PAPER View Journal | View Issue Selective oxidation of uronic acids into aldaric acids over gold catalyst† Cite this: RSC Adv.,2015,5,19502 a a a a b Sari Rautiainen, Petra Lehtinen, Jingjing Chen, Marko Vehkamaki,¨ Klaus Niemela,¨ a a Markku Leskel¨a and Timo Repo* Herein, uronic acids available from hemicelluloses and pectin were used as raw material for the synthesis of aldaric acids. Au/Al2O3 catalyst oxidized glucuronic and galacturonic acids quantitatively to the corresponding glucaric and galactaric acids at pH 8–10 and 40–60 C with oxygen as oxidant. The pH Received 29th January 2015 has a significant effect on the initial reaction rate as well as desorption of acid from the catalyst surface. Accepted 9th February 2015 At pH 10, a TOF value close to 8000 hÀ1 was measured for glucuronic acid oxidation. The apparent DOI: 10.1039/c5ra01802a activation energy Ea for glucuronic acid oxidation is dependent on the pH which can be attributed to the www.rsc.org/advances higher energy barrier for desorption of acids at lower pH. Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Introduction active. Metal oxide supported Au NPs, e.g. Au/Al2O3, have shown very high selectivity and long-term stability in oxidation of 20 Carbohydrates are abundant raw materials for the sustainable glucose to gluconic acid. Other aldoses are also selectively production of chemicals.1,2 Aldaric acids, diacids of sugars, are oxidized; however, the effect of carbohydrate structure on 16 important building block chemicals3 and have numerous reactivity is not quite clear.
    [Show full text]