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The Evolutionary Origins of Vertebrate Haematopoiesis: Insights from Non-Vertebrate Chordates
THE EVOLUTIONARY ORIGINS OF VERTEBRATE HAEMATOPOIESIS: INSIGHTS FROM NON-VERTEBRATE CHORDATES A thesis submitted to the University of Manchester for the degree of MPhil Evolutionary Biology in the Faculty of Life Sciences. 2015 PETER E D MILLS 2 LIST OF CONTENTS List of Contents 2 List of Figures 4 List of Abbreviations 5 Abstract 6 Declaration 7 Copyright Statement 7 Introduction 8 Haematopoiesis 11 In gnathostomes 11 In cyclostomes 18 In ascidians 20 In amphioxi 22 In sea urchins 24 In fruit flies 24 The evolution of haematopoiesis 26 Haematopoiesis and innate immune cells 26 Lymphocytes 26 Erythrocytes 27 Aims 28 The basic aim 28 Choice of genes 30 Homologue identification 31 Analysis of EST collections 31 In situ hybridisations 32 gata1/2/3 rescue experiment 34 gata1/2/3 reporter experiment 36 3 Methods 39 Homologue identification 39 Analysis of EST data 39 Collection of amphioxus embryos 40 Collection of ascidian embryos 40 Collection of zebrafish embryos 40 Cloning genes 41 Whole mount in situ hybridisations 41 Knockdown and rescue of gata1 42 gata1/2/3:mCherry reporter 42 Results Homologues in non-gnathostomes 44 Gene expression in ascidian and amphioxus embryos 48 Knockdown of gata1 and rescue with gata1/2/3 51 gata1/2/3:mCherry reporter expression 52 Discussion Non-chordate gene expression 54 Amphioxus gene expression 54 Ascidian gene expression 56 Cyclostome gene expression 59 gata1/2/3 rescue experiment 60 gata1/2/3 reporter experiment 62 Evolution of erythrocytes 63 Evolution of lymphocytes 67 Conclusion 72 References 75 (Word count: 26,246) 4 LIST OF FIGURES Figure 1 – Phylogenetic relationships between the organisms studied in this thesis. -
Age, Growth, and Sexual Maturity of the Deepsea Skate, Bathyraja
AGE, GROWTH, AND SEXUAL MATURITY OF THE DEEPSEA SKATE, BATHYRAJA ABYSSICOLA A Thesis Presented to the Faculty of Alaska Pacific University In Partial Fulfillment of the Requirements For the Degree of Master of Science in Environmental Science by Cameron Murray Provost April 2016 Pro Q u est Nu m b er: 10104548 All rig hts reserv e d INF O RM ATI O N T O ALL USERS Th e q u a lity of this re pro d u ctio n is d e p e n d e nt u p o n th e q u a lity of th e c o p y su b mitt e d. In th e unlik e ly e v e nt th a t th e a uth or did n ot se n d a c o m ple t e m a nuscript a n d th ere are missin g p a g es, th ese will b e n ot e d. Also, if m a t eria l h a d to b e re m o v e d, a n ot e will in dic a t e th e d e le tio n. Pro Q u est 10104548 Pu blish e d b y Pro Q u est LL C (2016). C o p yrig ht of th e Dissert a tio n is h e ld b y th e A uth or. All rig hts reserv e d. This w ork is prot e ct e d a g a inst un a uth orize d c o p yin g un d er Title 17, Unit e d St a t es C o d e Microform Editio n © Pro Q u est LL C . -
Barndoor Skate, Dipturus Laevis, Life History and Habitat Characteristics
NOAA Technical Memorandum NMFS-NE-173 Essential Fish Habitat Source Document: Barndoor Skate, Dipturus laevis, Life History and Habitat Characteristics U. S. DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration National Marine Fisheries Service Northeast Region Northeast Fisheries Science Center Woods Hole, Massachusetts March 2003 Recent Issues in This Series: 155. Food of Northwest Atlantic Fishes and Two Common Species of Squid. By Ray E. Bowman, Charles E. Stillwell, William L. Michaels, and Marvin D. Grosslein. January 2000. xiv + 138 p., 1 fig., 7 tables, 2 app. NTIS Access. No. PB2000-106735. 156. Proceedings of the Summer Flounder Aging Workshop, 1-2 February 1999, Woods Hole, Massachusetts. By George R. Bolz, James Patrick Monaghan, Jr., Kathy L. Lang, Randall W. Gregory, and Jay M. Burnett. May 2000. v + 15 p., 5 figs., 5 tables. NTIS Access. No. PB2000-107403. 157. Contaminant Levels in Muscle of Four Species of Recreational Fish from the New York Bight Apex. By Ashok D. Deshpande, Andrew F.J. Draxler, Vincent S. Zdanowicz, Mary E. Schrock, Anthony J. Paulson, Thomas W. Finneran, Beth L. Sharack, Kathy Corbo, Linda Arlen, Elizabeth A. Leimburg, Bruce W. Dockum, Robert A. Pikanowski, Brian May, and Lisa B. Rosman. June 2000. xxii + 99 p., 6 figs., 80 tables, 3 app., glossary. NTIS Access. No. PB2001-107346. 158. A Framework for Monitoring and Assessing Socioeconomics and Governance of Large Marine Ecosystems. By Jon G. Sutinen, editor, with contributors (listed alphabetically) Patricia Clay, Christopher L. Dyer, Steven F. Edwards, John Gates, Tom A. Grigalunas, Timothy Hennessey, Lawrence Juda, Andrew W. Kitts, Philip N. -
Extinction Risk and Conservation of the World's Sharks and Rays
RESEARCH ARTICLE elife.elifesciences.org Extinction risk and conservation of the world’s sharks and rays Nicholas K Dulvy1,2*, Sarah L Fowler3, John A Musick4, Rachel D Cavanagh5, Peter M Kyne6, Lucy R Harrison1,2, John K Carlson7, Lindsay NK Davidson1,2, Sonja V Fordham8, Malcolm P Francis9, Caroline M Pollock10, Colin A Simpfendorfer11,12, George H Burgess13, Kent E Carpenter14,15, Leonard JV Compagno16, David A Ebert17, Claudine Gibson3, Michelle R Heupel18, Suzanne R Livingstone19, Jonnell C Sanciangco14,15, John D Stevens20, Sarah Valenti3, William T White20 1IUCN Species Survival Commission Shark Specialist Group, Department of Biological Sciences, Simon Fraser University, Burnaby, Canada; 2Earth to Ocean Research Group, Department of Biological Sciences, Simon Fraser University, Burnaby, Canada; 3IUCN Species Survival Commission Shark Specialist Group, NatureBureau International, Newbury, United Kingdom; 4Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, United States; 5British Antarctic Survey, Natural Environment Research Council, Cambridge, United Kingdom; 6Research Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, Australia; 7Southeast Fisheries Science Center, NOAA/National Marine Fisheries Service, Panama City, United States; 8Shark Advocates International, The Ocean Foundation, Washington, DC, United States; 9National Institute of Water and Atmospheric Research, Wellington, New Zealand; 10Global Species Programme, International Union for the Conservation -
Skates and Rays Diversity, Exploration and Conservation – Case-Study of the Thornback Ray, Raja Clavata
UNIVERSIDADE DE LISBOA FACULDADE DE CIÊNCIAS DEPARTAMENTO DE BIOLOGIA ANIMAL SKATES AND RAYS DIVERSITY, EXPLORATION AND CONSERVATION – CASE-STUDY OF THE THORNBACK RAY, RAJA CLAVATA Bárbara Marques Serra Pereira Doutoramento em Ciências do Mar 2010 UNIVERSIDADE DE LISBOA FACULDADE DE CIÊNCIAS DEPARTAMENTO DE BIOLOGIA ANIMAL SKATES AND RAYS DIVERSITY, EXPLORATION AND CONSERVATION – CASE-STUDY OF THE THORNBACK RAY, RAJA CLAVATA Bárbara Marques Serra Pereira Tese orientada por Professor Auxiliar com Agregação Leonel Serrano Gordo e Investigadora Auxiliar Ivone Figueiredo Doutoramento em Ciências do Mar 2010 The research reported in this thesis was carried out at the Instituto de Investigação das Pescas e do Mar (IPIMAR - INRB), Unidade de Recursos Marinhos e Sustentabilidade. This research was funded by Fundação para a Ciência e a Tecnologia (FCT) through a PhD grant (SFRH/BD/23777/2005) and the research project EU Data Collection/DCR (PNAB). Skates and rays diversity, exploration and conservation | Table of Contents Table of Contents List of Figures ............................................................................................................................. i List of Tables ............................................................................................................................. v List of Abbreviations ............................................................................................................. viii Agradecimentos ........................................................................................................................ -
A Practical Guide to Effective Design and Management of Mpas For
A PRACTICAL GUIDE TO THE REPORT EFFECTIVE DESIGN AND 2019 MANAGEMENT OF MPAs FOR SHARKS AND RAYS This project has been a collaboration between the Centre LEAD AUTHOR: for Sustainable Tropical Fisheries and Aquaculture Cassandra L Rigby, James Cook (CSTFA) at James Cook University, Australia, and WWF. University ABOUT WWF AUTHORS: WWF is one of the largest and most experienced Colin Simpendorfer, James Cook independent conservation organizations, with over University 5 million supporters and a global network active in Andy Cornish, WWF-Hong Kong more than 100 countries. WWF´s mission is to stop the degradation of the planet´s natural environment and to build a future in which humans live in harmony with HOW TO CITE THIS WORK: nature, by conserving the world´s biological diversity, Rigby, C.L., Simpfendorfer, C.A. ensuring that the use of renewable resources is and A. Cornish (2019) A Practical sustainable, and promoting the reduction of pollution Guide to Effective Design and and wasteful consumption. WWF works to reverse Management of MPAs for Sharks declining shark populations through Sharks: Restoring and Rays. WWF, Gland, Switzerland. the Balance, a global initiative. www.panda.org DESIGN AND PRODUCTION: sharks.panda.org Evan Jeffries, Catherine Perry – Swim2Birds Ltd ABOUT CSTFA www.swim2birds.co.uk Research within the Centre for Sustainable Tropical Fisheries and Aquaculture (CSTFA) focuses not only Published in May 2019 by WWF on the aquatic and aquaculture systems that produce – World Wide Fund for Nature, food, but also the industries and communities that Gland, Switzerland utilise them. Multidisciplinary collaborations between our researchers provide the synergies to address Any reproduction in full or part substantial research problems in a way that individual must mention the title and credit research groups cannot. -
A Systematic Revision of the South American Freshwater Stingrays (Chondrichthyes: Potamotrygonidae) (Batoidei, Myliobatiformes, Phylogeny, Biogeography)
W&M ScholarWorks Dissertations, Theses, and Masters Projects Theses, Dissertations, & Master Projects 1985 A systematic revision of the South American freshwater stingrays (chondrichthyes: potamotrygonidae) (batoidei, myliobatiformes, phylogeny, biogeography) Ricardo de Souza Rosa College of William and Mary - Virginia Institute of Marine Science Follow this and additional works at: https://scholarworks.wm.edu/etd Part of the Fresh Water Studies Commons, Oceanography Commons, and the Zoology Commons Recommended Citation Rosa, Ricardo de Souza, "A systematic revision of the South American freshwater stingrays (chondrichthyes: potamotrygonidae) (batoidei, myliobatiformes, phylogeny, biogeography)" (1985). Dissertations, Theses, and Masters Projects. Paper 1539616831. https://dx.doi.org/doi:10.25773/v5-6ts0-6v68 This Dissertation is brought to you for free and open access by the Theses, Dissertations, & Master Projects at W&M ScholarWorks. It has been accepted for inclusion in Dissertations, Theses, and Masters Projects by an authorized administrator of W&M ScholarWorks. For more information, please contact [email protected]. INFORMATION TO USERS This reproduction was made from a copy of a document sent to us for microfilming. While the most advanced technology has been used to photograph and reproduce this document, the quality of the reproduction is heavily dependent upon the quality of the material submitted. The following explanation of techniques is provided to help clarify markings or notations which may appear on this reproduction. 1.The sign or “target” for pages apparently lacking from the document photographed is “Missing Pagefs)”. If it was possible to obtain the missing page(s) or section, they are spliced into the film along with adjacent pages. This may have necessitated cutting through an image and duplicating adjacent pages to assure complete continuity. -
Outcome of HELCOM MARITIME 17-2017
Baltic Marine Environment Protection Commission Maritime Working Group MARITIME 17-2017 St. Petersburg, Russia, 10-12 October 2017 OUTCOME OF THE 17TH MEETING OF THE MARITIME WORKING GROUP (MARITIME 17-2017) Table of Contents Introduction………………………………………………………………………………………………………………………………………………….2 Agenda Item 1 Adoption of the Agenda ................................................................................................ 2 Agenda Item 2 Matters arising from HELCOM meetings ....................................................................... 2 Agenda Item 3 Ballast water .................................................................................................................. 4 Agenda Item 4 Airborne emissions from ships and related measures .................................................. 5 Agenda Item 5 Sewage discharges from ships and port reception facilities ......................................... 7 Agenda Item 6 Other ship-generated wastes and port reception facilities (including marine litter) ... 8 Agenda Item 7 Anti-fouling systems .................................................................................................... 10 Agenda Item 8 Detection, investigation and prosecution of offenders of anti-pollution regulations 10 Agenda Item 9 Safety of navigation and related matters .................................................................... 10 Agenda Item 10 HELCOM publications, implementation and reporting ............................................... 11 Agenda Item 11 Activities within other -
Spatial Ecology and Fisheries Interactions of Rajidae in the Uk
UNIVERSITY OF SOUTHAMPTON FACULTY OF NATURAL AND ENVIRONMENTAL SCIENCES Ocean and Earth Sciences SPATIAL ECOLOGY AND FISHERIES INTERACTIONS OF RAJIDAE IN THE UK Samantha Jane Simpson Thesis for the degree of DOCTOR OF PHILOSOPHY APRIL 2018 UNIVERSITY OF SOUTHAMPTON 1 2 UNIVERSITY OF SOUTHAMPTON ABSTRACT FACULTY OF NATURAL AND ENVIRONMENTAL SCIENCES Ocean and Earth Sciences Doctor of Philosophy FINE-SCALE SPATIAL ECOLOGY AND FISHERIES INTERACTIONS OF RAJIDAE IN UK WATERS by Samantha Jane Simpson The spatial occurrence of a species is a fundamental part of its ecology, playing a role in shaping the evolution of its life history, driving population level processes and species interactions. Within this spatial occurrence, species may show a tendency to occupy areas with particular abiotic or biotic factors, known as a habitat association. In addition some species have the capacity to select preferred habitat at a particular time and, when species are sympatric, resource partitioning can allow their coexistence and reduce competition among them. The Rajidae (skate) are cryptic benthic mesopredators, which bury in the sediment for extended periods of time with some species inhabiting turbid coastal waters in higher latitudes. Consequently, identifying skate fine-scale spatial ecology is challenging and has lacked detailed study, despite them being commercially important species in the UK, as well as being at risk of population decline due to overfishing. This research aimed to examine the fine-scale spatial occurrence, habitat selection and resource partitioning among the four skates across a coastal area off Plymouth, UK, in the western English Channel. In addition, I investigated the interaction of Rajidae with commercial fisheries to determine if interactions between species were different and whether existing management measures are effective. -
Revised Palette of Measures for Reducing Phosphorus and Nitrogen Losses from Agriculture
Revised Palette of measures for reducing phosphorus and nitrogen losses from agriculture This document is a part of the 2013 HELCOM Ministerial Declaration and was endorsed by the 2013 HELCOM Ministerial Meeting Baltic Marine Environment Protection Commission REVISED LIST OF EXAMPLES (PALETTE) OF MEASURES FOR REDUCING PHOSPHORUS AND NITROGEN LOSSES FROM AGRICULTURE The document is based on the original “Examples of measures for reducing phosphorus and nitrogen losses from agriculture” adopted by the HELCOM Ministerial Meeting held in Krakow, Poland on 15 November 2007 and is updated with relevant regional and European experience and knowledge on application of agri-environmental measures the Baltic Sea catchment. It is intended to support implementation of part II Annex III of the 1992 Helsinki Convention Helsinki Convention “Criteria and measures concerning the prevention of pollution from land- based sources”. The Palette contains technical, managerial and legislative measures, based on best available knowledge and sought to help in implementation of the aforementioned provisions through e.g. review and further implementation of programmes for river basin management. Application of measures and their relative cost-efficiency is often case-specific and is subject to national considerations when selecting specific measures for application. The Palette will be reviewed by the expert community within HELCOM Baltic Agriculture and Environment Forum to reflect changes and development of those measures, including new knowledge when it becomes available. The Palette was compiled on the basis of the following main materials: 1. EU JRC Report on Pilot River Basins Network On Agricultural Issues (2008-2009): Sharing experiences and views 2. Comparative Study of Pressures and Measures in the Major River Basin Management Plans 3. -
DEEP SEA LEBANON RESULTS of the 2016 EXPEDITION EXPLORING SUBMARINE CANYONS Towards Deep-Sea Conservation in Lebanon Project
DEEP SEA LEBANON RESULTS OF THE 2016 EXPEDITION EXPLORING SUBMARINE CANYONS Towards Deep-Sea Conservation in Lebanon Project March 2018 DEEP SEA LEBANON RESULTS OF THE 2016 EXPEDITION EXPLORING SUBMARINE CANYONS Towards Deep-Sea Conservation in Lebanon Project Citation: Aguilar, R., García, S., Perry, A.L., Alvarez, H., Blanco, J., Bitar, G. 2018. 2016 Deep-sea Lebanon Expedition: Exploring Submarine Canyons. Oceana, Madrid. 94 p. DOI: 10.31230/osf.io/34cb9 Based on an official request from Lebanon’s Ministry of Environment back in 2013, Oceana has planned and carried out an expedition to survey Lebanese deep-sea canyons and escarpments. Cover: Cerianthus membranaceus © OCEANA All photos are © OCEANA Index 06 Introduction 11 Methods 16 Results 44 Areas 12 Rov surveys 16 Habitat types 44 Tarablus/Batroun 14 Infaunal surveys 16 Coralligenous habitat 44 Jounieh 14 Oceanographic and rhodolith/maërl 45 St. George beds measurements 46 Beirut 19 Sandy bottoms 15 Data analyses 46 Sayniq 15 Collaborations 20 Sandy-muddy bottoms 20 Rocky bottoms 22 Canyon heads 22 Bathyal muds 24 Species 27 Fishes 29 Crustaceans 30 Echinoderms 31 Cnidarians 36 Sponges 38 Molluscs 40 Bryozoans 40 Brachiopods 42 Tunicates 42 Annelids 42 Foraminifera 42 Algae | Deep sea Lebanon OCEANA 47 Human 50 Discussion and 68 Annex 1 85 Annex 2 impacts conclusions 68 Table A1. List of 85 Methodology for 47 Marine litter 51 Main expedition species identified assesing relative 49 Fisheries findings 84 Table A2. List conservation interest of 49 Other observations 52 Key community of threatened types and their species identified survey areas ecological importanc 84 Figure A1. -
Stock Assessment and Fishery Evaluation of Skate Species (Rajidae)
16. Gulf of Alaska Skates by Sarah Gaichas1, Nick Sagalkin2, Chris Gburski1, Duane Stevenson1, and Rob Swanson3 1NMFS Alaska Fisheries Science Center, Seattle WA 2ADF&G Commercial Fisheries Division, Kodiak AK 3NMFS Alaska Fisheries Science Center, Kodiak AK Executive Summary Summary of Major Changes Changes in the input data: 1. Total catch weight for GOA skates is updated with 2004 and partial 2005 data. 2. Biomass estimates from the 2005 GOA bottom trawl survey are incorporated. 3. Life history information has been updated with recent research results. 4. Information on the position of skates within the GOA ecosystem and the potential ecosystem effects of skate removals are included. Changes in assessment methodology: There are no changes to the Tier 5 assessment methodology. Changes in assessment results: No directed fishing for skates in the GOA is recommended, due to high incidental catch in groundfish and halibut fisheries. Skate biomass changed between the last NMFS GOA trawl survey in 2003 and the most recent survey in 2005, which changes the Tier 5 assessment results based on survey biomass. The recommendations for 2005 based on the three most recent survey biomass estimates for skates and M=0.10 are: Western Central GOA Eastern GOA GOA (610) (620, 630) (640, 650) Bathyraja skates Gulfwide Big skate ABC 695 2,250 599 ABC 1,617 OFL 927 3,001 798 OFL 2,156 Longnose skate ABC 65 1,969 861 OFL 87 2,625 1,148 Responses to SSC Comments SSC comments specific to the GOA Skates assessment: From the December, 2004 SSC minutes: The SSC is grateful to samplers with ADF&G who collected catch data and biological samples for Kodiak landings.