NMHS Progress Report (Period April 2019 to March 2020)
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NMHS Progress Report (Period April 2019 to March 2020) 1. Project Information Project ID: NMHS/2017-18/LG09/02 Sanction Date: Dated 22.12.2017 Conservation of Threatened Vertebrate Fauna in Indian Himalayan Region through Long-Term Monitoring and Project Title: Capacity Building BTG: Biodiversity Conservation and Management Dr. Kailash Chandra PI and Affiliation Zoological Survey of India, Prani Vigyan Bhawan (Institution): M-Block, New Alipore, Kolkata-700053, West Bengal Name & Address of the Co-PI, if ----NA---- any: In the Indian Himalayan Region (IHR) the Long-Term Monitoring Programs and capacity-building will aid in conservation and management of threatened vertebrates. Hence, to achieve the same in the project, we used a combination of different methods/ Structured techniques including sign surveys, camera trapping, non-invasive Abstract - DNA analysis as well as questionnaire surveys. The activities of the detailing the project started during April 2019. The intensive sampling was current year progress [Word conducted in all six districts/sites in IHR. Limit 250 words]: All the six sites (districts) were divided into 10×10 km grids and further 5×5 km girds for intensive sampling. The reconnaissance surveys were conducted to know the presence/absence and abundance of various vertebrate species. Further intensive areas were identified for long term and intensive monitoring of the faunal 1 | P a g e groups based on considering weather conditions, terrain, and/or availability of logistics into account. A total of 437 trails covering with an effort of 3848 km has been made, which resulted in collection of 3550 noninvasive samples. Furthermore, a total of 18864 camera trap night, yielded detection of 59 different vertebrate species. About 1560 samples has been processed for DNA analysis during the period. Furthermore, climate change prediction for Galliformes, Himalayan Langur and Brown bear were performed and predicted the future distribution. Based on the preliminary analysis, 10 species have been selected for the detail population genetics, demographic, phylogeography and to predict the future distribution under the climate change scenario. Among which phylogeography of the Ibex, landscape and population genetics of Red panda have been performed. Further, demographic history of Arunachal Macaque and other species have also been performed. Information on ethno-zoological and other socio-economics aspects was collected through questionnaire surveys in 318 villages and interviewed 3804 respondents at all 6 sites. Eight state level training workshops and one national level stakeholder workshops conducted. So far 8 manuscript published as an output of the project. One booklet on the threaten vertebrates of Indian Himalayan regions is in press which included the information about the species distribution, ecology, conservation threats and other major issue for 39 mammals, 49 birds, 19 fish, 4 amphibians and 15 reptiles. Flyers and other species specific information material have also been prepared for the awareness creation among the locals. Project Partner Name Affiliations Role & Responsibilities -----NIL----- -----NIL----- -----NIL----- 2 | P a g e 2. Project Site Details Lahaul East West Project Site Uttarkashi Darjeeling East Siang & Spiti Sikkim Kameng IHR States North Arunachal Arunachal HP Uttarakhand Sikkim Covered Bengal Pradesh Pradesh Long & Entire Entire Entire Entire Entire Entire district Lat. district district district district district Site Map Annexure-I (Detailed technical Report) Site Annexure-I (Detailed technical Report) Photograph 3. Project Activities Chart w.r.t. Timeframe [Gantt or PERT] Project Output Work Undertaken Activity Year 2019 - 2020 Qtr 1 Qtr 2 Qtr 3 Qtr 4 Objective 1 1. Procurement 1. Meeting with 1. Further 1. Awareness was 1. The objective of related, the State Forest intensive animal created among completed where To assess and chemicals, and Department for sign surveys and villagers, majority of monitor consumables. procuring camera trapping stakeholders and marked grids have threatened permissions for conducted and others and been covered with vertebrate 2. Preparation the Year-II and about 350 camera meeting with extensive fauna in the spatial submission of traps placed at Forest sampling in the IHR and distribution report to the different sites. Department five study areas of developing maps. different state Lahaul and Spiti, spatial forest Uttarkashi 3. Field based 2. Intensive 2. Continued the database department; Darjeeling, East data collected camera trapping, camera trapping Siang and East from the sign survey, and animal sign 2. Systematic Sikkim, for the different sites. other field based surveys in all the data collection Camera trapping, from the data were sites except the 4. Recruitment sign survey and different sites of collected from Lahaul and Spiti. of the project questionnaire 10 x10 km Lahaul and Spiti Staff for vacant survey and project staff grids; 5x5 Km noninvasive positions. based on the 3.Data entry, sample collection. study area size 3 | P a g e Project Output Work Undertaken Activity Year 2019 - 2020 writing and 2. A total of 623 3. Intensive compiling of camera traps has camera trapping, Research paper been deployed sign survey and and internal which resulted to DNA based evaluation 18864 camera study. meeting. trap night, yielding 59 DNA extraction Recruitment of individual from the the project staff vertebrate species Noninvasive for vacant from all study samples positions. areas. collected from the different 3. Developed the study sites. spatial data base for the threaten 6. Preparation of vertebrates of Geo-spatial IHR and Booklet layers is ‘in press’ with all relevant information. (Please see Detailed technical report attached for more details). Objective 2 1. Sample 2. A total of 741 1. Sample 1. A total 693 1. A total of 3550 collection has samples has collection was in samples collected animal samples so To study the been started in been collected progress at all six so far. A total of far collected from population Lahaul and from Lahaul and proposed sites in 496 samples has all the six sites in viability of Spiti, Himachal Spiti, Darjeeling the IHR. A total also been IHR. selected Pradesh. A total and East Siang of 567 non- processed for threatened of 192 samples districts in the invasive samples DNA extraction 2. DNA extraction species in IHR have been IHR. of different and further of 1672 samples for developing collected from animals have been genetic analysis. Sambar, Goral, long-term the area. collected. Species got Snow leopard, conservation identified using Brown bear, strategies 2. DNA 2. Analysis of 2. DNA extraction Black bear, Blue species genetic analysis. extraction and and PCR Species specific sheep, Ibex and occurrence data Primer amplification of markers have other species. from the Lahaul selection and samples for been identified procurement of and Spiti to population Demographic and identify the and primer got population primers for the viability and synthesized. different conservation population genetics analysis priority areas 4. Performed the of Red Panda, 4 | P a g e Project Output Work Undertaken Activity Year 2019 - 2020 species. genetics analysis. impact of climate Ibex, Blue sheep, change data Arunachal 3. Collection of analysis for Macaque and detail data on Galliformes, other related the different 3. DNA sequencing of Brown bear and species have been habitat Himalayan langur documented. about 471 parameters, in the Indian preparation of samples from the Apart from this, different study Himalayan raster files and regions and molecular other GIS sites, identify the taxonomy of IHR species, DNA predict the future related work. distribution. mammals also sequence quality been conducted check and for the 9 ungulate performed other species using the bioinformatics mitochondrial analysis. CYT B gene. (Please see detailed technical report attached for more details). Objective 3: 1. Long term 1. Field sign 1. Field sign 1. By conducting 1. Partially monitoring surveys and surveys, camera the field surveys, achieved. In the To set long- plots camera trapping trapping, sample potential areas are most of the sites term established in conducted in collection and being identified to we have identified monitoring the Lahaul and Darjeeling, East Socioeconomic be chosen as plots the long term protocol for Spiti, Sikkim, data collected for long-term monitoring plots threatened Uttarkashi, Uttarkashi, West from the all the monitoring. and repetitive vertebrate Darjeeling Kameng, study sites. samples from faunal groups based on the districts. 2. Around five these areas also in different preliminary 2. One manuscript areas have been been started from parts of IHR data analysis. 2. Analysis in were published on identified in the Lahaul and and to develop comparing Monitoring of Uttarkashi, 10 in Spiti and adaptive different mammals of Lahaul and Spiti, Uttarkashi, management monitoring trans-Himalaya 5 in Darjeeling Darjeeling. strategies for protocols of region and and 5 in East long-term mammals another was to Siang, and 5 in 2. Established the conservation distributed in the identifying the West Kameng. effecting of those Darjeeling and conservation monitoring species Lahaul and Spiti priority areas for protocols using through district. the brown bear in the Camera community the Lahaul valley. trapping and engagement noninvasive genetics 5 | P a g e Project Output Work Undertaken Activity Year 2019 - 2020 sampling. Objective 4: 1 General