Microfunnel Filter Funnels

™ MicroFunnel Filter Funnels Specifications Instructions for Use Materials of Construction Filter the Sample: Filter Media: GN-6 Metricel (mixed cellulose esters) Membrane 1. Assemble Manifold, Vacuum Flask Trap, Vacuum Pump, and Vacushield™ filter as Description Supor 200 (polyethersulfone) Membrane shown in Figure 1. Metricel Black (modified polyethersulfone) Membrane 2. Remove the funnel from the bag. Place funnel directly onto the Pall Life The MicroFunnel product is a disposable filter unit designed to recover and culture Support Pad: Cellulose Sciences aluminum manifold. If you do not have a Pall Life Sciences aluminum microorganisms from potable water, process water, and other aqueous samples. It Funnel, Base, LP Petri dish base: Polypropylene manifold, insert funnel adapter into no. 8 stopper, then place stopper/adapter will convert into its own Petri dish after filtration, or the membrane can be easily Funnel Cover: Polystyrene assembly into manifold or flask. removed and cultured in a separate Petri dish or on an agar plate. Plug: Polyethylene Funnel Adapter: Polyethylene (not autoclavable) 3. Remove cover (A) without touching the inner surface. Rinse inside walls of funnel and wet out membrane surface. Replace cover and turn on vacuum to draw Membrane Diameter rinse fluid through. Turn off vacuum. Ordering Information 47 mm 4. Remove cover (A) without touching the inner surface and pour sample into the Part Number Description Pkg Effective Filtration Area funnel. Replace cover . ® 13.46 cm2 4800 GN-6 Metricel Membrane, gridded, 0.45 µm, 100 mL, 50/pkg 5. Turn on the vacuum and draw the sample through the filter. Filter the entire sample Individually Bagged Maximum Vacuum first, then rinse the inside walls of the funnel with 60 mL of sterile buffer solution. 4801 GN-6 Metricel Membrane, gridded, 0.45 µm, 100 mL 50/pkg 635 mm Hg (25 in. Hg) (Vacuum Use Only) Allow the vacuum to remain for 30-45 seconds, then turn the vacuum off. 4804 GN-6 Metricel Membrane, gridded, 0.45 µm, 100 mL, 200/pkg Gamma Irradiation Information Culture the Sample: Individually Bagged Validated Dosage: 15-30 kGY (1.5-3.0 MRad) NOTE: To culture in base/lid Petri dish follow Method A. To remove the membrane 4810* GN-6 Metricel Membrane, gridded, 0.45 µm, 100 mL, 50/pkg Dimensions and culture the sample on an agar plate or Petri dish/pad, follow Method B or C. Individually Bagged 100 mL Capacity: Part references may be found in Figure 2. Method C part references may be found in Figure 3. MicroFunnel 300 mL capacity filter units cannot convert to a Petri dish 4826 GN-6 Metricel Membrane, plain, 0.45 µm, 100 mL, 50/pkg Height: 8.05 cm (3.17 in.) with lid kit and must be cultured using Method B. Individually Bagged 7.62 cm (3.00 in.) with cover 7.32 cm (2.88 in.) without cover Method A (Top Loading the Media) 4815 GN-6 Metricel Membrane, gridded, 0.45 µm, 300 mL, 20/pkg 1. Twist open the ampoule media of choice and discard the cap. Individually Bagged Diameter: 6.40 cm (2.52 in.) with lid kit 6.05 cm (2.38 in.) with cover 2. Remove the cover (A) from the funnel and gently squeeze the ampoule to 4805 Metricel Black Membrane, gridded, 0.45 µm, 100 mL, 50/pkg 5.74 cm (2.26 in.) without cover dispense the medium evenly onto the membrane surface. Use caution to avoid Individually Bagged 300 mL Capacity: excessive splashing and subsequent loss of the media onto the inside of the 4827 Metricel Black Membrane, plain, 0.45 µm, 100 mL, 50/pkg funnel cylinder. Individually Bagged Height: 9.09 cm (3.58 in.) with cover 8.94 cm (3.52 in.) without cover 3. Briefly apply vacuum to absorb the medium into the absorbent pad, using 4817 Metricel Black Membrane, gridded, 0.45 µm, 300 mL, 20/pkg caution to minimize the loss of the fluid volume. Turn off the vacuum, and Individually Bagged Diameter: 8.84 cm (3.48 in.) with cover replace the cover onto the filter unit. 8.53 cm (3.36 in.) without cover 4819 Metricel Black Membrane, gridded, 0.8 µm, 300 mL, 20/pkg 4. Remove the MicroFunnel filter unit from the manifold, and cap the media port Individually Bagged Typical Thickness with the plug (F) supplied. Remove the cover and separate the cylinder (B) from GN-6 Metricel Membrane 4803 Supor® Membrane, gridded, 0.2 µm, 100 mL, 50/pkg the base (E) by gently squeezing the funnel cylinder (B) near the top. Disengage 0.45 µm: 6 mils (152 µm) Individually Bagged the cylinder, and transfer the cover to the Petri base. Discard the funnel cylinder. Metricel Black Membrane 4806 Supor Membrane, plain, 0.2 µm, 100 mL, 50/pkg 5. Transfer the inverted Petri base to an incubator, and incubate (inverted) at the 0.45 µm: 5.1 mils (130 µm) Individually Bagged specified temperature and time. 0.8 µm: 5.8 mils (147 µm) 4818 Supor Membrane, gridded, 0.2 µm, 300 mL, 20/pkg 6. Count the colonies using a binocular microscope at 15X. Supor Membrane Individually Bagged 0.2 µm: 5.7 mils (145 µm) 7. Discard the Petri dish following appropriate federal, state, and local guidelines 4852 Supor Membrane, gridded, 0.45 µm, 100 mL, 50/pkg 0.45 µm: 5.5 mils (140 µm) for disposal of biohazardous material. Individually Bagged Recovery % in Comparison to Pour/Spread Plate 8. The MicroFunnel filter unit is designed for SINGLE USE ONLY. 4828 Supor Membrane, gridded, 0.45 µm, 300 mL, 20/pkg GN-6 Metricel Membrane Method B (Transferring the Membrane) Individually Bagged 0.45 µm: ≥ 90 1. Hold MicroFunnel filter unit with one hand and gently squeeze the funnel cylinder near the top. Carefully remove the funnel cylinder (B) from the base (E). * MicroFunnel filter funnel with lid petri dish Metricel Black Membrane 0.45 µm: ≥ 85 2. Dip the tips of the stainless steel forceps into alcohol and flame to sterilize. 0.8 µm: ≥ 85 3. Insert forceps and remove the membrane from the base leaving behind the Membranes Supor Membrane support pad. 0.2 µm: ≥ 90 4. Place the membrane filter on a prepared agar plate or Petri dish/absorbent pad GN-6 Metricel Membrane: A 0.45 µm mixed cellulose ester membrane used in 0.45 µm: ≥ 90 the Membrane Filter (MF) Technique for the determination of Total Coliforms, combination soaked with broth. E. coli, yeast, mold, Streptococcal strains, and total bacterial contamination. Typical Water Flow Rate 5. Follow steps 5-8 from Method A. mL/min/cm2 at 0.7 bar ( 70 kPa, 10 psi) Supor Membrane: 0.2 or 0.45 µm polyethersulfone membrane ideal for low Method C (For Use with PN 4810) binding of antibiotics or other inhibitory substances. GN-6 Metricel Membrane 1. Remove lid Petri dish (G) from top of funnel and dispense 2 mL ampoule media 0.45 µm: > 65 Metricel Black Membrane: 0.45 or 0.8 µm modified polyethersul fone membrane onto dry pad. used with the MF Technique to provide better contrast to light colored colonies of Metricel Black Membrane 2. Hold MicroFunnel filter unit with one hand and gently squeeze the funnel near bacteria, yeast and mold. 0.45 µm: > 34 the top. Carefully remove the funnel cylinder (B) from the base (E). 0.8 µm: > 102 The membrane contained in MicroFunnel filter funnels has been manufactured for 3. Dip the tips of the stainless steel forceps into alcohol and flame to sterilize. Supor Membrane microbiological analysis in accordance with the Membrane Filtration procedures 4. Insert forceps and remove the membrane from the base leaving behind the 0.2 µm: > 19 referenced in “Standard Methods for the Examination of Water and Wastewater,” support pad. 0.45 µm: > 38 Current Edition, and the US Environmental Protection Agency’s “Microbiological 5. Place the membrane filter on the broth-soaked pad in the lid Petri dish (G). Methods for Monitoring the Environment,” 600/8-78-017. 6. Follow steps 5-8 from Method A. • Recovery testing has shown that the grid lines do not enhance or inhibit growth of organisms. Culturing Coliform Organisms: NOTE: The Top Loading method of culturing is not recommended for culturing • The sterilization process did not enhance or inhibit subsequent growth of coliforms using MF-Endo Broth ampoule media. To obtain maximum sheen organisms. production, remove the membrane to a broth-soaked pad or agar MF-Endo • Recovery of Pseudomonas aeruginosa for 0.2 µm Supor membrane, total and medium as described in Method B. fecal coliform for 0.45 µm GN-6 Metricel membrane, and Saccharomyces cerevisiae for 0.45 and 0.8 µm Metricel Black membrane indicates that there is no influence on microbial growth and development due to chemical extractables. Instructions for Use (continued) WARNING Employment of the products in applications not specified, or failure to follow Figure 1 all instructions contained in this product information insert, may result in Filtration manifold setup detailing various options of attaching filter units to manifold. improper functioning of the product, personal injury, or damage to property or the product. See Statement of Warranty in our most recent catalog. OPTION 3 OPTION 2 OPTION 1 ATTENTION Adapter PN 4701 (sold separately) L’utilisation de nos produits dans des applications pour lesquelles ils ne Adapter (autoclavable) sont pas spécifiés ou le non-respect du mode d’emploi qui figure sur ce (disposable) document, peut entrainer un disfonctionnement du produit, endommager le produit ou d’autres biens matériels ou représenter un risque pour l’utilisateur. AttachesAttaches No. 8 Stopper directly to Se référer à la clause de garantie de notre catalogue le plus récent. directly to No.8 Stopper manifoldmanifold ACHTUNG Der Einsatz dieses Produktes in Anwendungen für die es nicht spezifiziert ist, Vacuum Source oder das Nichtbeachten einiger, in dieser Bedienungsanleitung gegebenen or Vacuum Pump Hinweise kann zu einem schlechteren Ergebnis, oder Zerstörung des Produktes oder anderer Dinge oder gar zu Verletzungen führen. Beachten Sie auch unsere Garantiebedingungen im aktuellen Katalog.

ADVERTENCIA El uso de este producto en aplicaciones no especificadas o el no considerar Vacushield las instrucciones indicadas en la hoja de información del producto puede vent device ocasionar un mal funcionamiento del producto, daños en las instalaciones o en el producto y riesgo para el personal del laboratorio. Consulte el apartado de Garantía en nuestro último catálogo.

ATTENZIONE Figure 2 Complementary Products L’impiego dei prodotti in applicazioni non specificate, o il mancato rispetto Product Nos. 4800, 4801, 4803, 4805, 4806, 4815, 4817, 4818* Part Number Description Pkg di tutte le istruzioni contenute nel presente bollettino tecnico, potrebbero portare ad un utilizzo improprio del prodotto, ferire gli operatori, o danneggiare Ampoule Media le caratteristiche del prodotto stesso. Consultare la dichiarazione di garanzia Funnel Cover (A) in plastic ampoules, 2 mL: pubblicata nel nostro più recente catalogo. Funnel Cover (A) 4302 M-FC Broth with rosolic acid, Fecal Coliform 50/pkg 4306 Pseudomonas Broth, Pseudomonas sp. 50/pkg 4307 Trypticase Soy Broth-USP, Total Bacteria 50/pkg Funnel Cylinder (B) Membrane (C) 4352 HPC Broth with TTC indicator, Total Bacteria 50/pkg Cellulose 68105 MF-Endo Broth, Total Coliform 50/pkg Absorbent Pad (D) Membrane (C) 68106 M-TGE Broth, Total Bacteria 50/pkg Funnel Base (E) 68107 M-Green YM Broth, Yeast and Mold 50/pkg Cellulose Absorbent Pad (D) 68108 KF Streptococcal Broth, Fecal Streptococcus 50/pkg Plug (F) 68109 Orange Serum Broth, Acidophilic Bacteria 50/pkg Funnel Base (E) in wide mouth glass ampoules, 2 mL: 68100 M-FC Broth, Fecal Coliform 20/pkg Petri Dish Conversion 68101 M-FC Broth with rosolic acid, Fecal Coliform 20/pkg 100 mL Funnels only 68102 MF-Endo Broth, Total Coliform 20/pkg in 100 mL bottles: 4313 MF-Endo Broth, Total Coliform 1/pkg Figure 3 Hardware and Accessories Product No. 4810 15402 3-Port Aluminum Manifold 1/pkg 15403 6-Port Aluminum Manifold 1/pkg Funnel Cover (A) Funnel Cover (A) 13157 Vacuum/Pressure Pump, 115 V 1/pkg 13158 Vacuum/Pressure Pump, 230 V CE 1/pkg Cellulose Membrane (C) Absorbent Pad (D) 51147 Stainless Steel Forceps, Black 1/pkg Cellulose 4690 Stainless Steel Forceps, Multicolor 3/pkg Lid Petri Dish (G) Absorbent Pad (D) 4701 Autoclavable Funnel Adapter 3/pkg Pall Life Sciences Australia, Cheltenham, VIC, 03 9584 8100 Austria, Wien, 00 1 49 192 0 Funnel Lid Petri Dish (G) 82728 No. 8 Rubber Stopper 1/pkg 600 South Wagner Road Cylinder (B) Ann Arbor, MI 48103-9019 USA Canada, Ontario, 905-542-0330 4402 Vacushield Vent Device 3/pkg Canada, Québec, 514-332-7255 4713 Vacuum Pump Funnel Adapter 1/pkg 800.521.1520 USA and Canada China, P.R., Beijing, 86-10-6780 2288 (+)800.PALL.LIFE Outside USA and Canada France, St. Germain-en-Laye, 01 30 61 32 32 Use of Lid Petri Dish Membrane (C) Petri Dishes and Absorbent Pads 734.665.0651 phone Germany, Dreieich, 06103-307 333 7245 Petri dish with Absorbent Pads, 50 mm, sterile 100/pkg 734.913.6114 fax India, Mumbai, 91 (0) 22 67995555 Italy, Buccinasco, +3902488870.1 Cellulose 7242 Petri dish without Absorbent Pads, 50 mm, sterile 100/pkg Absorbent Pad (D) Japan, Tokyo, 03-6901-5800 66025 Absorbent Pads with Dispenser, 47 mm, sterile 1000/pkg Visit us on the Web at www.pall.com/lab Korea, Seoul, 82-2-560-8711 Funnel Base (E) E-mail us at [email protected] Malaysia, Selangor, +60 3 5569 4892 Poland, Warszawa, 22 510 2100 Russia, Moscow, 5 01 787 76 14 Singapore, 65 6 389-6500 South Africa, Johannesburg, +27-11-2662300 Spain, Madrid, 91-657-9876 Sweden, Lund, (0)46 158400 Switzerland, Basel, 061-638 39 00 Pall, , Metricel, MicroFunnel, Supor, and Taiwan, Taipei, 886 2 2545 5991 Vacushield are trademarks of Pall Corporation. Thailand, Bangkok, 66 2937 1055 ® indicates a registered trademark in the USA. United Kingdom, Farlington, 02392 302600