Electronic Supplementary Material (ESI) for RSC Advances. This journal is © The Royal Society of Chemistry 2017

Table S1 Total components of PO saponification wastewater

Chemical name Percentage CAS# Water 94.35％ 7732-18-5 Dichloroisopropyl ether 0.05％ 108-60-1 Methyl dichloroacetate 0.03％ 116-54-1 1,7-Dichloro-4-oxaheptane 0.04％ 629-36-7 2,4-Dimethyl-2-pentanol 0.08％ 625-06-9 Calcium hypochlorite 0.02％ 7778-54-3 Calcium chloride 5.39％ 7774-34-7 Sodium sulfate 0.03％ 7757-82-6 Sodium hydroxide 0.01％ 1310-73-2 Table S2 The main phylum of in PO saponification wastewater treatment plants

Abundance in aeration Abundance in contact Phylum tank oxidation tank Q value for variance* GN04 3 1089 0 Proteobacteria 22171 12646 2.55116E-311 Firmicutes 176 997 3.1213E-192 Chlorobi 191 780 6.9433E-129 Chloroflexi 418 24 4.74769E-51 Spirochaetes 0 119 1.63935E-43 TM6 31 100 5.59127E-15 5324 4375 2.49964E-13 Tenericutes 1 39 1.64055E-11 Cyanobacteria 34 89 3.75193E-11 OD1 4 37 2.5175E-09 Gemmatimonadetes 2 31 2.30322E-08 Dictyoglomi 0 12 0.000419747 Planctomycetes 2 19 0.000484315 NKB19 0 11 0.00085437 Acidobacteria 0 15 0.001055658 Actinobacteria 911 704 1 Chlamydiae 162 98 1 TM7 12 5 1 Verrucomicrobia 22 12 1 *Q value between the two groups was calculated using Metastats. Table S3 The main class of bacteria in PO saponification wastewater treatment plants

Abundance in aeration Class tank Abundance in contact oxidation tank Alphaproteobacteria 17897 7004 Bacteroidia 4020 1433 Gammaproteobacteria 3946 4747 Rhodothermi 998 378 Actinobacteria 599 375 Chloroflexi 415 1 Acidimicrobiia 204 291 Deltaproteobacteria 192 692 Ignavibacteria 184 688 Chlamydiia 162 98 Clostridia 155 759 136 2068 Saprospirae 131 72 Betaproteobacteria 123 80 Thermoleophilia 104 32 Cytophagia 38 420 Chloroplast 34 15 SJA-4 31 98 Pedosphaerae 22 11 Bacilli 21 238 Epsilonproteobacteria 13 114 TM7-3 11 4 OPB56 4 91 unclassified 4 47 GN15 3 1089 Gemm-5 1 25 Mollicutes 1 39 Dehalococcoidetes 0 22 Dictyoglomia 0 12 Leptospirae 0 24 Oscillatoriophycideae 0 23 Phycisphaerae 0 16 Solibacteres 0 14 Spirochaetes 0 95 Synechococcophycideae 0 44 Table S4 The main genera of bacteria in PO saponification wastewater treatment plants

Abundance in Abundance in contact oxidation Genera aeration tank tank Q value for variance* Thalassospira 0 836 5.77698E-312 Mesorhizobium 1847 305 7.3791E-154 Pseudidiomarina 0 303 7.0446E-111 Paracoccus 626 2 2.20378E-96 Marinobacter 2041 600 1.86356E-86 Methylophaga 562 9 2.20232E-83 Sporotomaculum 5 248 5.98278E-82 Owenweeksia 136 422 9.0241E-56 KSA1 934 234 2.65478E-48 Inquilinus 0 126 1.02498E-45 Aquicella 0 125 5.1539E-45 Sulfurimonas 0 101 4.00238E-36 Halothiobacillus 3 92 2.21144E-28 Francisella 54 174 1.30584E-23 Parvibaculum 0 51 1.2122E-17 Desulfovibrio 4 63 3.48597E-17 Novispirillum 307 417 1.1236E-16 Arenimonas 0 45 7.26332E-16 Alcanivorax 0 44 3.79764E-15 Lactococcus 1 47 7.77848E-15 Yonghaparkia 34 107 9.29268E-14 Paenibacillus 0 40 1.00115E-13 Persicobacter 13 69 6.72036E-13 Idiomarina 0 34 6.58686E-12 Clostridium 28 84 4.30259E-11 Chelativorans 236 64 4.43516E-11 Cryomorpha 0 31 7.59773E-11 Bdellovibrio 2 38 1.14871E-10 Desulfurispora 0 35 1.14871E-10 Rhodovulum 21 69 3.30191E-10 Demequina 0 29 3.6537E-10 Balneola 0 28 8.31668E-10 Aeromicrobium 72 5 3.40853E-08 Leptonema 0 24 5.60007E-08 Dehalogenimonas 0 22 1.27885E-07 Amycolatopsis 49 1 2.91499E-06 Spirulina 0 19 3.68205E-06 Lysobacter 0 16 4.41621E-05 Solibacillus 0 16 4.41621E-05 Wandonia 0 15 4.41621E-05 SC3-56 0 15 0.000101156 Devosia 430 210 0.00025821 Ammoniphilus 0 11 0.002934975 Anaerovorax 0 13 0.004401745 Oceanicaulis 0 10 0.006443324 Phaeospirillum 0 10 0.006443324 Clostridiisalibacter 79 23 0.008347593 Pseudonocardia 6 16 0.133543572 unclassified 21595 15529 1 Mycobacterium 259 157 1 Rhodococcus 18 6 1 Bacillus 13 19 1 Sphingomonas 11 14 1 Pseudomonas 9 14 1 Table S5 The main family of bacteria in PO saponification wastewater treatment plants

Abundance in Abundance in contact Family aeration tank oxidation tank Rhodobacteraceae 12793 550 Phyllobacteriaceae 2969 496 Alteromonadaceae 2041 603 Balneolaceae 934 262 Marinicellaceae 792 662 Rhodospirillaceae 709 1797 Piscirickettsiaceae 562 11 Hyphomicrobiaceae 445 304 YCB18 415 1 Mycobacteriaceae 259 157 Nocardioidaceae 213 32 Parachlamydiaceae 162 6 Ignavibacteriaceae 160 299 136 468 C111 121 71 Clostridiaceae 107 114 Alcaligenaceae 102 44 Coxiellaceae 90 157 Rhodothermaceae 63 116 Rickettsiaceae 60 307 Pseudonocardiaceae 55 17 Francisellaceae 54 632 Microbacteriaceae 43 119 Flammeovirgaceae 38 392 Erythrobacteraceae 32 247 Saprospiraceae 31 13 Ruminococcaceae 29 172 Beijerinckiaceae 27 304 OTUb7 24 130 Sphingomonadaceae 23 158 Pedosphaeraceae 22 2 Legionellaceae 21 141 Nocardiaceae 18 6 Bacillaceae 17 33 Microthrixaceae 16 94 Helicobacteraceae 13 111 Caulobacteraceae 11 9 Pseudomonadaceae 10 14 Aurantimonadaceae 9 11 Peptococcaceae 6 296 Desulfovibrionaceae 4 74 Halothiobacillaceae 3 92 Bdellovibrionaceae 2 38 Paenibacillaceae 1 122 Planococcaceae 1 22 Streptococcaceae 1 49 Acidaminobacteraceae 0 67 Alcanivoracaceae 0 44 Amoebophilaceae 0 19 Cellulomonadaceae 0 29 Chitinophagaceae 0 49 Dehalococcoidaceae 0 22 Desulfobacteraceae 0 17 Desulfobulbaceae 0 351 Dictyoglomaceae 0 12 Ectothiorhodospiraceae 0 41 Flavobacteriaceae 0 308 Hyphomonadaceae 0 196 Idiomarinaceae 0 337 Kiloniellaceae 0 836 Leptospiraceae 0 24 lheB3-7 0 247 Melioribacteraceae 0 11 Mogibacteriaceae 0 13 PAUC26f 0 14 Pseudanabaenaceae 0 44 SB-1 0 123 Simkaniaceae 0 90 Spirochaetaceae 0 95 Spirulinaceae 0 19 Xanthomonadaceae 0 68 Table S6 Distribution of the major unidentified OTUs and BLAST results (%) Contact Similari Aeration tank oxidation OTU ty (%) Genera tank A C E G I J OTU00 0.2999 0.2794 0.2712 0.2432 0.2809 0.003985 99 Roseovarius 01 61 06 05 91 72 OTU00 0.1361 0.1137 0.1098 0.1477 0.1212 0.044166 89 Cytophaga 02 14 65 89 94 12 OTU00 0.1025 0.0883 0.1000 0.0901 0.1102 Roseibacteriu 0.013051 98 03 82 56 29 84 17 m OTU00 0.0259 0.0271 0.0241 0.0317 0.0239 Mesorhizobiu 0.003059 99 06 23 22 55 76 92 m OTU00 0.0190 0.0313 0.0268 0.0315 0.0306 0.029518 --- 05 08 71 7 32 25 OTU00 0.0029 0.0030 0.0036 0.0036 0.0552 Halochromati 0.003852 93 15 96 49 65 02 09 um OTU00 8.06E- 1.62E- 0.0535 0 0 5.02E-05 96 Owenweeksia 19 06 05 46 OTU00 0.0001 6.4E- 3.08E- 0.0480 0 0.000116 --- 26 1 05 05 16 OTU00 2.93E- 5.62E- 0.0001 7.9E- 0.0230 4.13E-05 --- 41 05 05 32 05 51 OTU00 1.58E- 1.62E- 0.0209 0 0 0 91 Micavibrio 42 05 05 61