Rabbit Anti-Human STAM2 (Phospho-Tyr192) Polyclonal Antibody (CABT-L4115) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use

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Rabbit Anti-Human STAM2 (Phospho-Tyr192) polyclonal antibody (CABT-L4115) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview This antibody detects endogenous levels of STAM2 only when phosphorylated at Tyr192. Specificity Target Modification: Phospho. Modification Sites: Human: Y192; Mouse: Y192 Target Human STAM2 (Phospho-Tyr192) Immunogen The antiserum was produced against synthesized peptide derived from human STAM2 around the phosphorylation site of Tyr192. Immunogen range: 161-210 Isotype IgG Source/Host Rabbit Species Reactivity Human, Mouse Purification Affinity Purified Conjugate Unconjugated Applications WB, ELISA Molecular Weight 58 kDa Preparation The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. Format Liquid Concentration Lot specific Buffer Rabbit IgG in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol. Preservative 0.02% Sodium Azide Storage Stable at -20°C for at least 1 year. Ship Wet ice Warnings For research use only. 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved BACKGROUND Introduction The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation. Keywords STAM2;signal transducing adaptor molecule (SH3 domain and ITAM motif) 2;Hbp;STAM2A;STAM2B;signal transducing adapter molecule 2;STAM-2;HSE1 homolog;Hrs- binding protein;STAM-like protein containing SH3 and ITAM domains 2; GENE INFORMATION Gene Name STAM2 signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 [ Homo sapiens (human) ] Official Symbol STAM2 Synonyms HBP, HRS binding protein, Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2, Signal transducing adaptor molecule 2A, Signal transducing adaptor molecule 2B Entrez Gene ID 8986 UniProt ID O75886 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.

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1 Supporting Information for a Microrna Network Regulates
Supporting Information for A microRNA Network Regulates Expression and Biosynthesis of CFTR and CFTR-ΔF508 Shyam Ramachandrana,b, Philip H. Karpc, Peng Jiangc, Lynda S. Ostedgaardc, Amy E. Walza, John T. Fishere, Shaf Keshavjeeh, Kim A. Lennoxi, Ashley M. Jacobii, Scott D. Rosei, Mark A. Behlkei, Michael J. Welshb,c,d,g, Yi Xingb,c,f, Paul B. McCray Jr.a,b,c Author Affiliations: Department of Pediatricsa, Interdisciplinary Program in Geneticsb, Departments of Internal Medicinec, Molecular Physiology and Biophysicsd, Anatomy and Cell Biologye, Biomedical Engineeringf, Howard Hughes Medical Instituteg, Carver College of Medicine, University of Iowa, Iowa City, IA-52242 Division of Thoracic Surgeryh, Toronto General Hospital, University Health Network, University of Toronto, Toronto, Canada-M5G 2C4 Integrated DNA Technologiesi, Coralville, IA-52241 To whom correspondence should be addressed: Email: [email protected] (M.J.W.); yi- [email protected] (Y.X.); Email: [email protected] (P.B.M.) This PDF file includes: Materials and Methods References Fig. S1. miR-138 regulates SIN3A in a dose-dependent and site-specific manner. Fig. S2. miR-138 regulates endogenous SIN3A protein expression. Fig. S3. miR-138 regulates endogenous CFTR protein expression in Calu-3 cells. Fig. S4. miR-138 regulates endogenous CFTR protein expression in primary human airway epithelia. Fig. S5. miR-138 regulates CFTR expression in HeLa cells. Fig. S6. miR-138 regulates CFTR expression in HEK293T cells. Fig. S7. HeLa cells exhibit CFTR channel activity. Fig. S8. miR-138 improves CFTR processing. Fig. S9. miR-138 improves CFTR-ΔF508 processing. Fig. S10. SIN3A inhibition yields partial rescue of Cl- transport in CF epithelia.
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