Ecological Epigenetics in Timema Cristinae Stick Insects: on the Patterns, Mechanisms and Ecological Consequences of DNA Methylation in the Wild
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Ecomorph Convergence in Stick Insects (Phasmatodea) with Emphasis on the Lonchodinae of Papua New Guinea
Brigham Young University BYU ScholarsArchive Theses and Dissertations 2018-07-01 Ecomorph Convergence in Stick Insects (Phasmatodea) with Emphasis on the Lonchodinae of Papua New Guinea Yelena Marlese Pacheco Brigham Young University Follow this and additional works at: https://scholarsarchive.byu.edu/etd Part of the Life Sciences Commons BYU ScholarsArchive Citation Pacheco, Yelena Marlese, "Ecomorph Convergence in Stick Insects (Phasmatodea) with Emphasis on the Lonchodinae of Papua New Guinea" (2018). Theses and Dissertations. 7444. https://scholarsarchive.byu.edu/etd/7444 This Thesis is brought to you for free and open access by BYU ScholarsArchive. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of BYU ScholarsArchive. For more information, please contact [email protected], [email protected]. Ecomorph Convergence in Stick Insects (Phasmatodea) with Emphasis on the Lonchodinae of Papua New Guinea Yelena Marlese Pacheco A thesis submitted to the faculty of Brigham Young University in partial fulfillment of the requirements for the degree of Master of Science Michael F. Whiting, Chair Sven Bradler Seth M. Bybee Steven D. Leavitt Department of Biology Brigham Young University Copyright © 2018 Yelena Marlese Pacheco All Rights Reserved ABSTRACT Ecomorph Convergence in Stick Insects (Phasmatodea) with Emphasis on the Lonchodinae of Papua New Guinea Yelena Marlese Pacheco Department of Biology, BYU Master of Science Phasmatodea exhibit a variety of cryptic ecomorphs associated with various microhabitats. Multiple ecomorphs are present in the stick insect fauna from Papua New Guinea, including the tree lobster, spiny, and long slender forms. While ecomorphs have long been recognized in phasmids, there has yet to be an attempt to objectively define and study the evolution of these ecomorphs. -
Insecta: Phasmatodea) and Their Phylogeny
insects Article Three Complete Mitochondrial Genomes of Orestes guangxiensis, Peruphasma schultei, and Phryganistria guangxiensis (Insecta: Phasmatodea) and Their Phylogeny Ke-Ke Xu 1, Qing-Ping Chen 1, Sam Pedro Galilee Ayivi 1 , Jia-Yin Guan 1, Kenneth B. Storey 2, Dan-Na Yu 1,3 and Jia-Yong Zhang 1,3,* 1 College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China; [email protected] (K.-K.X.); [email protected] (Q.-P.C.); [email protected] (S.P.G.A.); [email protected] (J.-Y.G.); [email protected] (D.-N.Y.) 2 Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada; [email protected] 3 Key Lab of Wildlife Biotechnology, Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, China * Correspondence: [email protected] or [email protected] Simple Summary: Twenty-seven complete mitochondrial genomes of Phasmatodea have been published in the NCBI. To shed light on the intra-ordinal and inter-ordinal relationships among Phas- matodea, more mitochondrial genomes of stick insects are used to explore mitogenome structures and clarify the disputes regarding the phylogenetic relationships among Phasmatodea. We sequence and annotate the first acquired complete mitochondrial genome from the family Pseudophasmati- dae (Peruphasma schultei), the first reported mitochondrial genome from the genus Phryganistria Citation: Xu, K.-K.; Chen, Q.-P.; Ayivi, of Phasmatidae (P. guangxiensis), and the complete mitochondrial genome of Orestes guangxiensis S.P.G.; Guan, J.-Y.; Storey, K.B.; Yu, belonging to the family Heteropterygidae. We analyze the gene composition and the structure D.-N.; Zhang, J.-Y. -
De Novo Characterization of the Timema Cristinae Transcriptome Facilitates Marker Discovery and Inference of Genetic Divergence
Molecular Ecology Resources (2012) doi: 10.1111/j.1755-0998.2012.03121.x De novo characterization of the Timema cristinae transcriptome facilitates marker discovery and inference of genetic divergence AARON A. COMEAULT,* MATHEW SOMMERS,* TANJA SCHWANDER,† C. ALEX BUERKLE,‡ TIMOTHY E. FARKAS,* PATRIK NOSIL* and THOMAS L. PARCHMAN‡ *Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, CO 80303, USA, †Center for Ecology and Evolutionary Studies, University of Groningen, 9700CC Groningen, The Netherlands, ‡Department of Botany, University of Wyoming, Laramie, WY 82071, USA Abstract Adaptation to different ecological environments can promote speciation. Although numerous examples of such ‘ecological speciation’ now exist, the genomic basis of the process, and the role of gene flow in it, remains less understood. This is, at least in part, because systems that are well characterized in terms of their ecology often lack genomic resources. In this study, we characterize the transcriptome of Timema cristinae stick insects, a system that has been researched intensively in terms of ecological speciation, but for which genomic resources have not been previously developed. Specifically, we obtained >1 million 454 sequencing readsthatassembledinto84937contigsrepresenting approximately 18 282 unique genes and tens of thousands of potential molecular markers. Second, as an illustration of their utility, we used these geno- mic resources to assess multilocus genetic divergence within both an ecotype pair and a species pair of Timema stick insects. The results suggest variable levels of genetic divergence and gene flow among taxon pairs and genes and illustrate afirststeptowardsfuturegenomicworkinTimema. Keywords: gene flow, isolation with migration, next-generation sequencing, speciation, transcriptome Received 3 November 2011; revision received 6 January 2012; accepted 13 January 2012 Introduction resources (some notable exceptions aside, such as three- spine stickleback; Peichel et al. -
Phasmida (Stick and Leaf Insects)
● Phasmida (Stick and leaf insects) Class Insecta Order Phasmida Number of families 8 Photo: A leaf insect (Phyllium bioculatum) in Japan. (Photo by ©Ron Austing/Photo Researchers, Inc. Reproduced by permission.) Evolution and systematics Anareolatae. The Timematodea has only one family, the The oldest fossil specimens of Phasmida date to the Tri- Timematidae (1 genus, 21 species). These small stick insects assic period—as long ago as 225 million years. Relatively few are not typical phasmids, having the ability to jump, unlike fossil species have been found, and they include doubtful almost all other species in the order. It is questionable whether records. Occasionally a puzzle to entomologists, the Phasmida they are indeed phasmids, and phylogenetic research is not (whose name derives from a Greek word meaning “appari- conclusive. Studies relating to phylogeny are scarce and lim- tion”) comprise stick and leaf insects, generally accepted as ited in scope. The eggs of each phasmid are distinctive and orthopteroid insects. Other alternatives have been proposed, are important in classification of these insects. however. There are about 3,000 species of phasmids, although in this understudied order this number probably includes about 30% as yet unidentified synonyms (repeated descrip- Physical characteristics tions). Numerous species still await formal description. Stick insects range in length from Timema cristinae at 0.46 in (11.6 mm) to Phobaeticus kirbyi at 12.9 in (328 mm), or 21.5 Extant species usually are divided into eight families, in (546 mm) with legs outstretched. Numerous phasmid “gi- though some researchers cite just two, based on a reluctance ants” easily rank as the world’s longest insects. -
Epigenetics Mechanisms in Insects: a Review
Int.J.Curr.Microbiol.App.Sci (2020) 9(5): 2961-2971 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 5 (2020) Journal homepage: http://www.ijcmas.com Review Article https://doi.org/10.20546/ijcmas.2020.905.339 Epigenetics Mechanisms in Insects: A Review U. Pirithiraj1, R. P. Soundararajan2* and C. Gailce Leo Justin1 1Anbil Dharmalingam Agricultural College and Research Institute, Tiruchirappalli-620 027, India 2Horticultural College and Research Institute (Women), Tiruchirappalli-620 027, India *Corresponding author ABSTRACT Epigenetics is the study of heritable changes in gene expression that do not involve changes in the underlying DNA sequence (or) a change in phenotype without a change in genotype. Phenotype is K e yw or ds the observable or measurable characteristics of an organism i.e., height, behaviour, colour, shape, and size. Insects are being examined for their epigenetic phenomena and the underlying mechanism Epigenetics, Insects, behind it. Epigenetics is well studied in fruit fly, Drosophila melanogaster. Gene is segments of the DNA methylation, DNA sequence that store the information to synthesize proteins or RNAs that carry out specific Histone functions. Epigenetics is important in cellular differentiation which is responsible for the phenotypic modification and plasticity. Epigenetics is been investigated in plants and animals. On comparing other organisms RNAi insects possess a high degree of phenotypic variation. This variation is due to switch on and off mechanism of gene. Gene expression and repression in insects is regulated through epigenetic Article Info mechanisms i.e. DNA Methylation, Histone modification and Noncoding RNAs which influence the Accepted: phenotypic modification. -
Entertainment-Education in Science Education Available on Mobile Devices Interactive Tasks Allow You to Quickly Verify the Acquired Knowledge
Entertainment-education in science education the monograph edited by Grzegorz Karwasz & Małgorzata Nodzyńska 1 2 Entertainment-education in science education the monograph edited by Grzegorz Karwasz & Małgorzata Nodzyńska TORUŃ 2017 3 The monograph edited by: Grzegorz Karwasz & Małgorzata Nodzyńska Rewievers: Cover: Ewelina Kobylańska ISBN .......... 4 Introduction Jan Amos Komensky in „Great Didactics” (Amsterdam, 1657) defined didactics not as a mere process of teaching, but as teaching efficient, lasting and pleasant. He wrote (p. 131) “The school itself should be a pleasant place, and attractive to the eye both within and without. […] If this is done, boys will, in all probability, go to school with as much pleasure as to fairs, where they always hope to see and hear something new.” Further (p. 167) Komensky added: “The desire to know and to learn should be excited in boys in every possible manner.” The idea of linking the fun with didactics finds many followers, expressed also in tittles of activities like “Science is Fun” or “Physics is Fun”. In (Karwasz, Kruk, 2012) we defined three complementary aspects of any bit of information (an exhibition object, a film, a lecture): entertainment (“ludico” in Italian), didactics, and science. The first aspect gives an impression to a student/ visitor/ listener: “how funny it is!”. The didactical aspect induces: “How simple it is!” And the aspect of scientific curiosity induces in best students a question: “How complex it is!” These three functions add-up like three basic colors to give a full spectrum of enlightenment. The entertainment function can be performed in different forms – school, extra-school, complementary to school. -
Phasmid Studies ISSN 0966-0011 Volume 9, Numbers 1 & 2
Phasmid Studies ISSN 0966-0011 volume 9, numbers 1 & 2. Contents Species Report PSG. 122, Anisomorpha monstrosa Hebard Paul A. Hoskisson . 1 Cigarrophasma, a new genus of stick-insect (Phasmatidae) from Australia Paul D. Brock & Jack Hasenpusch . 0 •••••• 0 ••• 0 ••••••• 4 A review of the genus Medaura Stal, 1875 (Phasmatidae: Phasmatinae), including the description of a new species from Bangladesh Paul Do Brock & Nicolas Cliquennois 11 First records and discovery of two new species of Anisomorpha Gray (Phasmida: Pseudophasmatidae) in Haiti and Dominican Republic Daniel E. Perez-Gelabert 0 .. .. 0 • • • • • • 0 • • • • 0 • • 0 • 0 • • 0 0 • • • 27 Species report on Pharnacia biceps Redtenbacher, PSG 203 Wim Potvin 0 ••• 28 How Anisomorpha got its stripes? Paul Hoskisson . 33 Reviews and Abstracts Book Reviews . 35 Phasmid Abstracts 38 Cover illustr ation : Orthonecroscia pulcherrima Kirby, drawing by PoE. Bragg. Species Report PSG. 122, Anisomorpha monstrosa Hebard Paul A. Hoskisson, School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, 13 3AF, UK. With illustrations by P.E. Bragg. Abstract This report summarises the care and breeding of Anisomorpha monstrosa Hebard, the largest species in the genus. Behaviour and defence mechanism are also discussed along with descriptions of the eggs, nymphs, and adults. Key words Phasmida, Anisomorpha monstrosa, Pseudophasmatinae, Rearing, Distribution, Defence. Taxonomy Anisomorpha monstrosa belongs to the sub-family Pseudophasmatinae. It was described in 1932 by Hebard (1932: 214) and is the largest species in the genus. The type specimen is a female collected from Merida, in Yucatan, Mexico. Culture History The original culture of this species was collected in Belize, approximately 150km north of Belize City by Jan Meerman in 1993 or 1994 (D'Hulster, personal communication). -
DNA Methylation Suppresses Chitin Degradation and Promotes the Wing
Xu et al. Epigenetics & Chromatin (2020) 13:34 https://doi.org/10.1186/s13072-020-00356-6 Epigenetics & Chromatin RESEARCH Open Access DNA methylation suppresses chitin degradation and promotes the wing development by inhibiting Bmara-mediated chitinase expression in the silkworm, Bombyx mori Guanfeng Xu1,2†, Yangqin Yi1,2†, Hao Lyu1,2, Chengcheng Gong1,2, Qili Feng1,2, Qisheng Song3, Xuezhen Peng1,2, Lin Liu1,2 and Sichun Zheng1,2* Abstract Background: DNA methylation, as an essential epigenetic modifcation found in mammals and plants, has been implicated to play an important role in insect reproduction. However, the functional role and the regulatory mecha- nism of DNA methylation during insect organ or tissue development are far from being clear. Results: Here, we found that DNA methylation inhibitor (5-aza-dC) treatment in newly molted pupae decreased the chitin content of pupal wing discs and adult wings and resulted in wing deformity of Bombyx mori. Transcriptome analysis revealed that the up-regulation of chitinase 10 (BmCHT10) gene might be related to the decrease of chitin content induced by 5-aza-dC treatment. Further, the luciferase activity assays demonstrated that DNA methylation suppressed the promoter activity of BmCHT10 by down-regulating the transcription factor, homeobox protein arau- can (Bmara). Electrophoretic mobility shift assay, DNA pull-down and chromatin immunoprecipitation demonstrated that Bmara directly bound to the BmCHT10 promoter. Therefore, DNA methylation is involved in keeping the structural integrity of the silkworm wings from unwanted chitin degradation, as a consequence, it promotes the wing develop- ment of B. mori. Conclusions: This study reveals that DNA methylation plays an important role in the wing development of B. -
Could Medauroidea Extradentata (Brunner Von Wattenwyl 1907) Survive in Provence (France)? Gabriel Olive, Gilles Olive
Could Medauroidea extradentata (Brunner von Wattenwyl 1907) survive in Provence (France)? Gabriel Olive, Gilles Olive To cite this version: Gabriel Olive, Gilles Olive. Could Medauroidea extradentata (Brunner von Wattenwyl 1907) survive in Provence (France)?. Phasmid Studies, Phasmid Studies Group, 2019, 20, pp.4-14. hal-01995824 HAL Id: hal-01995824 https://hal.archives-ouvertes.fr/hal-01995824 Submitted on 27 Jan 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Phasmid Studies 20 Could Medauroidea extradentata (Brunner von Wattenwyl 1907) survive in Provence (France)? Gabriel Olive and Gilles Olive École Industrielle et Commerciale de la Ville de Namur, Laboratoire C2A, 2B, Rue Pépin, B-5000 Namur, Belgium. [email protected] Abstract Several cases of accidental introduction of insects far away from their biotope have already been re- ported. Could Medauroidea extradentata, a stick insect originally from the district of Annam, Viet- nam, survive in Provence (France)? To answer this question, two sets of experiments were performed. In the first one, specimens were put together with five plants growing only in the Mediterranean areas (bear’s breeches (Acanthus mollis), almond tree (Prunus dulcis), fig blanche d’Argenteuil variety (Ficus carica), fig violette de Solliès variety (Ficus carica), olive (Olea europaea), Aleppo pine (Pinus halepensis)) to investigate their adaptability to these diets. -
Analysis of the Stick Insect (Clitarchus Hookeri) Genome Reveals a High Repeat Content and Sex- Biased Genes Associated with Reproduction Chen Wu1,2,3* , Victoria G
Wu et al. BMC Genomics (2017) 18:884 DOI 10.1186/s12864-017-4245-x RESEARCH ARTICLE Open Access Assembling large genomes: analysis of the stick insect (Clitarchus hookeri) genome reveals a high repeat content and sex- biased genes associated with reproduction Chen Wu1,2,3* , Victoria G. Twort1,2,4, Ross N. Crowhurst3, Richard D. Newcomb1,3 and Thomas R. Buckley1,2 Abstract Background: Stick insects (Phasmatodea) have a high incidence of parthenogenesis and other alternative reproductive strategies, yet the genetic basis of reproduction is poorly understood. Phasmatodea includes nearly 3000 species, yet only thegenomeofTimema cristinae has been published to date. Clitarchus hookeri is a geographical parthenogenetic stick insect distributed across New Zealand. Sexual reproduction dominates in northern habitats but is replaced by parthenogenesis in the south. Here, we present a de novo genome assembly of a female C. hookeri and use it to detect candidate genes associated with gamete production and development in females and males. We also explore the factors underlying large genome size in stick insects. Results: The C. hookeri genome assembly was 4.2 Gb, similar to the flow cytometry estimate, making it the second largest insect genome sequenced and assembled to date. Like the large genome of Locusta migratoria,the genome of C. hookeri is also highly repetitive and the predicted gene models are much longer than those from most other sequenced insect genomes, largely due to longer introns. Miniature inverted repeat transposable elements (MITEs), absent in the much smaller T. cristinae genome, is the most abundant repeat type in the C. hookeri genome assembly. -
10 Walking Sticks: Natural Selection for Cryptic Coloration on Different Host Plants
Case Studies in Ecology and Evolution DRAFT 10 Walking sticks: natural selection for cryptic coloration on different host plants While she was a graduate student at the University of California, Christina Sandoval discovered a new species of insect. Timema christinae is an inconspicuous stick insect that lives in the chaparral of Southern California. It is only about 2 cm long and it feeds mostly at night. During the day it remains still and hides by mimicking the branches and leaves of its host plant. Because they are such good mimics of the host plants they feed on they are called “stick insects” or “walking sticks”. Eggs hatch on ground and young climb into a nearby host plant. Sometimes they never leave that single plant. Despite their inactivity, Sandoval noticed some very interesting differences between the insects. There were two color types. Some of the walking sticks were plain green while the others had a long white stripe on their http://paradisereserve.ucnrs.org/Timem a.html back. Moreover, those two color morphs were associated with two different species of host plant, with one type found on one host plant and the other on the second host. One of the first possibilities she considered was that the two forms were different species. Sandoval brought them back to the lab and found that the two types could interbreed freely, which showed that they were simply color variants of a single species of walking stick. Why, then, were there two colors types? Why were they segregated on different host plant species? She suspected that this was an example of natural selection at work. -
Classification: Phamatodea
NOMINA INSECTA NEARCTICA 637 CLASSIFICATION The primary data comes from unpublished database files of the author. The classification is based on: Bradley, J.D., and B.S. Galil. 1977. The taxonomic arrangement of the Phasmatodea with keys to the subfamilies and tribes. Proceedings of the Entomological Society of Washington, 79:176-208. HETERONEMIIDAE Heteronemiinae: Diapheromera, Manomera, Megaphasma, Pseudosermyle, Sermyle. Pachymorphinae: Parabacillus. PHASMATIDAE Cladomorphinae: Aplopus. TIMEMATIDAE Timema. PSEUDOPHASMATIDAE Pseudophasmatinae: Anisomorpha. Family # Names # Valid Heteronemiidae 29 20 Phasmatidae 1 1 Pseudophasmatidae 5 2 Timematidae 10 10 Total 45 33 PHASMATODEA 638 NOMINA INSECTA NEARCTICA HETERONEMIIDAE Anisomorpha Gray 1835 Anisomorpha buprestoides Stoll 1813 (Phasma) Diapheromera Gray 1835 Phasma vermicularis Stoll 1813 Syn. Spectrum bivittatum Say 1828 Syn. Diapheromera arizonensis Caudell 1903 (Diapheromera) Phasma calamus Burmeister 1838 Syn. Diapheromera carolina Scudder 1901 (Diapheromera) Anisomorpha ferruginea Beauvois 1805 (Phasma) Diapheromera covilleae Rehn and Hebard 1909 (Diapheromera) Diapheromera femoratum Say 1824 (Spectrum) Diapheromera sayi Gray 1835 Syn. Bacunculus laevissimus Brunner 1907 Syn. Diapheromera persimilis Caudell 1904 (Diapheromera) TIMEMATIDAE Bacunculus texanus Brunner 1907 Syn. Diapheromera dolichocephala Brunner 1907 Syn. Diapheromera tamaulipensis Rehn 1909 (Diapheromera) Diapheromera torquata Hebard 1934 (Diapheromera) Timema Scudder 1895 Diapheromera velii Walsh 1864 (Diapheromera)