Family Genus Outgroup: Chalcidoidea Pteromalidae

Title Hybrid capture data unravel a rapid radiation of pimpliform parasitoid wasps (Hymenoptera: Ichneumonidae: Pimpliformes)

Authors Klopfstein, S; Langille, B; Spasojevic, T; Broad, G; Cooper, SJB; Austin, AD; Niehuis, O

Date Submitted 2020-09-01 Supplementary File S2. Taxon sampling including detailed collection data. From Klopfstein et al. - Hybrid capture data unravels a rapid radiation of pimpliform parasitoid wasps (Hymenoptera: Ichneumonidae: Pimpliformes). Systematic Entomology. Higher grouping (Sub)family Genus Outgroup: Chalcidoidea Pteromalidae Thaumasura Outgroup: Evanioidea Gasteruptiidae Gasteruption Outgroup: Braconidae Alysiinae Dacnusa Outgroup: Braconidae Aphidiinae Aphidius Outgroup: Braconidae Aphidiinae Diaeretus Outgroup: Braconidae Homolobinae Homolobus Outgroup: Braconidae Macrocentrinae Macrocentrus Outgroup: Braconidae Microgastrinae Cotesia Outgroup: Braconidae Rogadinae Aleiodes Xoridiformes Xoridinae Xorides Ophioniformes Anomaloninae Heteropelma Ophioniformes Banchinae Apophua Ophioniformes Campopleginae Campoplex Ophioniformes Campopleginae Hyposoter Ophioniformes Cremastinae Dimophora Ophioniformes Ctenopelmatinae Xenoschesis Ophioniformes Mesochorinae Astiphromma Ophioniformes Metopiinae Colpotrochia Ophioniformes Ophioninae Leptophion Ophioniformes Tersilochinae Diaparsis Ophioniformes Tryphoninae Netelia Ophioniformes Tryphoninae Netelia Labeniformes Labeninae Poecilocryptus Ichneumoniformes Alomyinae Alomya Ichneumoniformes Cryptinae Buathra Ichneumoniformes Ichneumoninae Ichneumon Pimpliformes Acaenitinae Arotes Pimpliformes Acaenitinae Coleocentrus Pimpliformes Acaenitinae Jezarotes Pimpliformes Acaenitinae Spilopteron Pimpliformes Acaenitinae Yezoceryx Pimpliformes Collyriinae Collyria Pimpliformes Cylloceriinae Cylloceria Pimpliformes Cylloceriinae Cylloceria Pimpliformes Diacritinae Diacritus Pimpliformes Diacritinae Diacritus Pimpliformes Diplazontinae Homotropus Pimpliformes Diplazontinae Sussaba Pimpliformes Diplazontinae Syphophilus Pimpliformes Diplazontinae Syrphophilus Pimpliformes Diplazontinae Tymmophorus Pimpliformes Orthocentrinae Eusterinx Pimpliformes Orthocentrinae Hemiphanes Pimpliformes Orthocentrinae Hemiphanes Pimpliformes Orthocentrinae Megastylus Pimpliformes Orthocentrinae Neurateles Pimpliformes Orthocentrinae Orthocentrus Pimpliformes Orthocentrinae Picrostigeus Pimpliformes Orthocentrinae Plectiscidea Pimpliformes Orthocentrinae Proclitus Pimpliformes Pimplinae Acrodactyla Pimpliformes Pimplinae Amazopimpla Pimpliformes Pimplinae Anastelgis Pimpliformes Pimplinae Apechthis Pimpliformes Pimplinae Atractogaster Pimpliformes Pimplinae Camptotypus Pimpliformes Pimplinae Delomerista Pimpliformes Pimplinae Dolichomitus Pimpliformes Pimplinae Dolichomitus Pimpliformes Pimplinae Echthromorpha Pimpliformes Pimplinae Flavopimpla Pimpliformes Pimplinae Liotryphon Pimpliformes Pimplinae Nomosphecia Pimpliformes Pimplinae Parema Pimpliformes Pimplinae Perithous Pimpliformes Pimplinae Pimpla Pimpliformes Pimplinae Pimpla Pimpliformes Pimplinae Piogaster Pimpliformes Pimplinae Polysphincta Pimpliformes Pimplinae Reclinervellus Pimpliformes Pimplinae Schizopyga Pimpliformes Pimplinae Theronia Pimpliformes Pimplinae Xanthopimpla Pimpliformes Pimplinae Zatypota Pimpliformes Pimplinae Zonopimpla Pimpliformes Poemeniinae Cnastis Pimpliformes Poemeniinae Deuteroxorides Pimpliformes Poemeniinae Eugalta Pimpliformes Poemeniinae Poemenia Pimpliformes Poemeniinae Pseudorhyssa Pimpliformes Rhyssinae Epirhyssa Pimpliformes Rhyssinae Rhyssa Pimpliformes Rhyssinae Triancyra

1 Data type: transcr = transcriptome, hybEnr = hybrid enrichment data 2 Repositories of voucher specimens: KYU = Department of Entomology, University of Kentucky, USA; NMBE = Natural History Museum Bern, Switzerland; NMBS = Natural History Museum Basel Switzerland; SMTP = Swedish Malaise Trap Project, Station Linné on Öland, Sweden; UTU = University of Turku, Finland; WC = Waite Insect collection, University of Adelaide, Australia. A dash indicates that no voucher specimens are available as they were ground for RNA extractions (transcriptome specimens that have a collection indicated in these cases are pseudo-voucher, i.e., identical morphospecies (para- or syngenophore) collected at the same localities). 3 Identified by: either the name of a person who identified the species or of an institution at which the identification was ascertained. 1KITE = 1k Insect Transcriptome Evolution project; SMTP = Swedish Malaise Trap Project. 4 Collected by: either the name of a person who collected the species or of an institution. SMTP = Swedish Malaise Trap Project. Supplementary File S2. Taxon sampling including detailed collection data. Hybrid capture data unravels a rapid radiation of pimpliform parasitoid wasps (Hymenoptera: Ichneumonidae: Pimpliformes). Systematic Entomology. Species Specimen ID# Data type1 Country Repository2 Sex Identified by3 sp. Ichn-2467 hybEnr AU WC f G. Gibson sp. Ichn-2470 hybEnr AU WC f S. Klopfstein sibirica 1KITE transcr DE - 1KITE colemani 1KITE transcr DE - 1KITE essigellae 1KITE transcr AU - R. Glatz sp. Ichn-2469 hybEnr AU WC f S. Klopfstein marginator 1KITE transcr SE - 1KITE vestalis 1KITE transcr NL - 1KITE testaceus 1KITE transcr FR - M. Shaw praecatorius transcr-SK12 transcr CH NMBS f S. Klopfstein amictum 1KITE transcr CH - S. Klopfstein evanescens transcr-SK09 transcr CH NMBS m S. Klopfstein capitator Ichn-2519 hybEnr FR NMBS f P. Louapre didymator 1KITE transcr FR - m 1KITE kentmartini Ichn-2465 hybEnr AU WC f S. Klopfstein fulvicornis Ichn-2359 hybEnr CH NMBS f S. Klopfstein sp. Ichn-2453 hybEnr US KYU m S. Klopfstein cincta transcr-SK10 transcr CH - f S. Klopfstein anici Ichn-2462 hybEnr AU WC f G. Broad sp. Ichn-2449 hybEnr CH NMBE m S. Klopfstein cf. melanura 1KITE transcr SE - f S. Klopfstein sp. 1KITE transcr HN - L. Krogmann cf. nigromaculatus Ichn-2466 hybEnr AU WC f S. Klopfstein debellator Ichn-2387 hybEnr CH NMBS m S. Klopfstein laborator 1KITE transcr SE NMBS f G. Broad albiger 1KITE transcr CH - f S. Klopfstein moiwanus Ichn-2341 hybEnr JP NMBS f M. Ito excitator transcr-SK01 transcr CH NMBE m S. Klopfstein tamanukii Ichn-2338 hybEnr JP NMBS f M. Ito occiputale Ichn-2507 hybEnr US NMBS f S. Klopfstein sp. Ichn-2509 hybEnr TH KYU f S. Klopfstein trichophthalma transcr-SK11 transcr CH - f S. Klopfstein melancholica Ichn-2131 hybEnr SE NMBS f S. Klopfstein cf. caligata Ichn-2524 hybEnr US KYU f G. Broad aciculatus Ichn-2143 hybEnr SE NMBS f S. Klopfstein aciculatus Ichn-2522 hybEnr SE NMBS f S. Klopfstein frontorius Ichn-2365 hybEnr CH NMBS f S. Klopfstein cognata Ichn-2513 hybEnr CH NMBS f S. Klopfstein tricinctorius 1KITE transcr CH - S. Klopfstein tricinctorius Ichn-2364 hybEnr CH NMBS f S. Klopfstein suspiciosus Ichn-2460 hybEnr CH NMBS f S. Klopfstein oligomera Ichn-2502 hybEnr SE NMBS ff G. Broad erratum Ichn-2523 hybEnr SE NMBS f G. Broad performidatum Ichn-2499 hybEnr SE NMBS m G. Broad flavopictus Ichn-2183 hybEnr SE NMBS f G. Broad sp. Ichn-2526 hybEnr SE NMBS ff G. Broad sp. Ichn-2500 hybEnr SE NMBS f G. Broad debilis Ichn-2498 hybEnr SE NMBS f G. Broad cf. tenuicornis Ichn-2503 hybEnr SE NMBS f S. Klopfstein nr. praetor Ichn-2510 hybEnr SE NMBS f G. Broad degener Ichn-2137 hybEnr SE NMBS f S. Klopfstein sp. Ichn-2455 hybEnr EC UTU m I. Sääksjärvi sp. Ichn-2454 hybEnr NI UTU f I. Sääksjärvi compunctor transcr-SK05 transcr CH - m S. Klopfstein semisculptus Ichn-2278 hybEnr SE SMTP m SMTP sp. Ichn-2434 hybEnr CG NMBS m S. Klopfstein mandibularis Ichn-2528 hybEnr SE KYU f S. Klopfstein imperator transcr-SK15 transcr CH - f S. Klopfstein quercicolus transcr-SK04 transcr CH NMBS m S. Klopfstein intricatoria Ichn-2464 hybEnr AU WC m S. Klopfstein nigromaculata Ichn-2458 hybEnr TH KYU f S. Klopfstein cf. punctulatus Ichn-2361 hybEnr CH NMBS m S. Klopfstein pyramida Ichn-2093 hybEnr TH KYU f S. Klopfstein nigrobalteata Ichn-2497 hybEnr TH KYU f S. Klopfstein scurra Ichn-2525 hybEnr SE SMTP f S. Klopfstein flavicoxis 1KITE transcr SE NMBS f G. Broad rufipes transcr-SK03 transcr CH - f S. Klopfstein pilosator Ichn-2144 hybEnr CH NMBE f S. Klopfstein tuberosa Ichn-2518 hybEnr SE NMBS f S. Korenko nielseni Ichn-2505 hybEnr CZ NMBS m S. Korenko frigida Ichn-2136 hybEnr SE NMBS f S. Klopfstein hilaris Ichn-2433 hybEnr US NMBS m S. Klopfstein varimaculata Ichn-2448 hybEnr TH KYU f S. Klopfstein percontatoria Ichn-2139 hybEnr SE NMBS f S. Klopfstein sp. Ichn-2457 hybEnr GU UTU m I. Sääksjärvi vulgaris Ichn-2354 hybEnr JP NMBS f M. Ito elevator transcr-SK07 transcr CH - f S. Klopfstein cf. strigosa Ichn-2442 hybEnr TH KYU m G. Broad hectica Ichn-2234 hybEnr SE NMBS f S. Klopfstein nigricornis Ichn-2344 hybEnr JP NMBS f M. Ito sp. Ichn-2074 hybEnr TH KYU f S. Klopfstein amoena transcr-SK08 transcr CH - f S. Klopfstein galloisi Ichn-2343 hybEnr JP NMBS m M. Ito

Repositories of voucher specimens: KYU = Department of Entomology, University of Kentucky, USA; NMBE = Natural History Museum Bern, Switzerland; NMBS = Natural History Museum Basel Switzerland; SMTP = Swedish Malaise Trap Project, Station Linné on Öland, Sweden; UTU = University of Turku, Finland; WC = Waite Insect collection, University of Adelaide, Australia. A dash indicates that no voucher specimens are available as they were ground for RNA extractions (transcriptome specimens that have a collection indicated in these cases are pseudo-voucher, i.e., identical morphospecies (para- or syngenophore) collected at the same localities). Identified by: either the name of a person who identified the species or of an institution at which the identification was ascertained. 1KITE = 1k Insect Transcriptome Evolution project; SMTP = Swedish Malaise Trap Project. Collected by: either the name of a person who collected the species or of an institution. SMTP = Swedish Malaise Trap Project. Locality Latitude AU, South Australia: Belair NP, Malaise trap AU, South Australia: between Vivonne Bay camp ground access road and Castle Rock. Swept from Correa sp. DE: Labstock, Sautter & Stepper, Rosenstrasse 19, 72119 Ammerbuch, Germany DE: Labstock, re-natur GmbH, Charles-Roa -Weg 24, 24601 Ruhwinkel AU, South Australia: SARDI Entomology Unit, Box 397 GPO. Adelaide SA 5001 AU, South Australia: Kersbrooks, Lot 113, Robertson Road, Malaise trap SE, Uppland: Hultmanstorp NL: Labstock, J. de Boer, Laboratory of Entomology, Wageningen University, Wageningen FR: Aude, Ponzois-Minervois. ex Eupithecia or near genus in capkins / young leaves of Quercus CH, Kt. Bern, Rüschegg, Eywald 46.75°N CH, Kt. Bern: Bremgartenwald close to Eymatt 46.967°N CH, Kt. Bern Eymatt, Gäbelbachdelta, Wald/Waldrand 46.95°N FR, Pyrénées-Orientales: Perpignan, collected in vineyard ("QB 27"). FR, Rennes: INRA, UMR 1349 IGEPP INRA, Agrocampus Ouest Rennes, Université de Rennes 1, BP35327, 35653 Le Rheu cedex AU, New South Wales: Crommellin Field Station, Malaise trap 33.550°S CH, VS: Hérémence to Les Agettes, Mayens de Sion 46.203°N USA, Kentucky, Berea College forest: Cowbell Creek Area Trap 1. K34 37.54445°N CH, Kt. Bern Roggwil, Mumenthaler Weier 47.25°N AU, South Australia: Witchelina, at light. Bush Blitz Survey SM022 30.022°S CH, Grisson: Alp Flix, Sur, NE Sur, Malaise trap 46°31.451N SE, Stockholms län: Torö, Herrhamra HN, Olancho: near La Union, Parque National La Muralla AU, Tasmania: Eaglehawk Neck, Dog Line, on galls of Acacia (coastal wattle), SE59 43.016°S CH, Kt. Bern: Gurnigel 46.729°N SE, Stockholm, Brunnsviken, Haga, Sambucus bush close to Wallenberglaboratoriet 59.366°N CH, Kt. Bern: Gurnigel, Wasserscheide 46.73°N JP, Hyogo-pref.: Yabu-city, Mt. Hyonosen, Oodanganaru CH, Kt. Bern, Oberwangen, Inselwald 46.9°N JP, Hyougo: Takarazuka C., Kizukinomori 34.844°N USA, Kentucky: Berea College Forest, Horse Cover Rd, Malaie trap 6 (K109) 37.575676°N TH, Chiang Mai: Doi Inthanon NP, Vachiratharn Falls (T175). Malaise trap 18.539°N CH, Kt. Bern, Rüschegg, Eywald 46.75°N SE, Lule lappmark : Gällivare kommun, Ätnarova försökspark, Pelttovaara, Malaise t 67.0517°N USA, Kentucky: Madison Co., The Bull, Malaise Trap 1 (forest edge) 37.882°N SE, Öland: Mörbylånga kommun, Västerstads almlund nature reserve, Malaise trap 56.4273°N SE, Öland: Mörbylånga kommun, Västerstads almlund nature reserve 56.4273°N CH, Kt. Lucerne: Entlebuch, Tag der Artenvielfalt, Heiligkreuz to Schüpfheim 46.933°N CH, Kt. Bern: Bremgartenwald Opening, Malaise trap A 46°57.577N CH, Kt. Bern: Bremgartenwald close to Eymatt 46.967°N CH, LU: Entlebuch, Tag der Artenvielfalt, Heiligkreuz to Schüpfheim 46.933°N CH, SO: Trimbach, Miesernbach, Malaise trap 2 47.365°N SE, Södermanland: Huddinge kommun, Sofielunds återvinningsanläggning, Malaise t59.1765°N SE, Dalarna: Säters kommun, Säterdalen, Näsåkerspussen 60.3667°N SE, Lule lappmark : Jokkmokks kommun, Muddus nationalpark, Malaise trap 66.7695°N SE, Uppland: Älvkarleby kommun, Båtfors, Malaise trap 60.4607°N SE, Dalarna: Säters kommun, Säterdalen, Näsåkerspussen, Malaise trap 60.3667°N SE, Dalarna: Säters kommun, Säterdalen, Näsåkerspussen, Malaise trap 60.3667°N SE, Dalarna: Säters kommun, Säterdalen, Näsåkerspussen, Malaise trap 60.3667°N SE, Hälsingland: Hudiksvalls kommun, Stensjön-Lomtjärn, Malaise trap 62.1403°N SE, Västerbotten: Vindelns kommun, Kulbäckslidens försökspark, barrskog, , Malaise64.1786°N SE, Halland : Kungsbacka kommun, Särö Västerskog, Malaise trap 57.5052°N EC, Dept. Orellana: Onkone Gare, canopy fogging Lot#1737 0.657°S NI, Jinctega: PN Cerro Soslaya, Montane wet forest, Malaise trap LLAMA#D-03-2-0213.7691°N CH, Kt. Bern Roggwil, Mumenthaler Weier 47.25°N SE, Södermanland: Haninge kommun, Tyresta, Urskogsslingan, Malaise trap 59.1759°N CD, Sangha Prov.: Bomassa, mixed forest with marathacae 2.21°N SE, Uppland: Håbo kommun, Biskops-Arnö 59.6721°N CH, Kt. Bern, Köniz, Könizbergwald 46.933°N CH, Kt. Bern, Rüschegg, Eywald 46.75°N AU, South Australia: Stirling, Woorabinda, Stirling Linear Park, Mt. Lofty Ranges, Malaise trap TH, Chaiyaphum: Pa Hin Ngam NP, Ecotone between mixed deciduous and dry diptero15.582°N CH, VS: Hérémence to Les Agettes, Mayens de Sion 46.203°N TH, Phetchabun: Khao Kho NP, Mixed deciduous forest at Ta Phol river. Malaise tra16.542°N TH, Loei: Phu Kradueng NP, Dry evergreen forest (Pong neep forest unit). Malaise t 16.943°N SE, Uppland: Uppsala kommun, Fiby urskog, Trefaldighets källa 59.8834°N SE, Stockholm, Brunnsviken, Haga, Sambucus bush close to Wallenberglaboratoriet 59.366°N CH, Kt. Bern, Rüschegg, Eywald 46.75°N CH, GR: Val Mustair SE: Botenosco ("BOTTE.H.ARAN_01") CR, KOL+PR Kaceni, Louka, Lit. Pohorgui. From host C.conica - spider remains left (pupa - 27.v.14, adult - 6.vii.14) SE, Lule lappmark : Gällivare kommun, Ätnarova försökspark, Pelttovaara, Malaise t 67.0517°N USA, Kentucky: Fayette Co. Horse Farm, Malaise Ttrap 5 (bush pile) 38.008°N TH, Phitsanulok: Thung Salaeng Luang NP, Staff house (Gang Sopa waterfall). Mala16.878°N SE, Öland : Mörbylånga kommun, Södra lunden (Ottenby), , Malaise trap 56.2194°N GT, Suchitepéq: 4km S Vol Atilan, cloud forest, Malaise trap LLAMA#Ma-B-09-1-02 14.54807°N JP, Fukui: Obama c., Negori, Onyu-Pass 35.386°N CH, Kt. Bern, Rüschegg, Eywald 46.75°N TH, Chiang Mai: Doi Inthanon NP, Checkpoint 2. Malaise trap (T1799) 18.526°N SE, Öland: Mörbylånga kommun, Västerstads almlund nature reserve, Malaise trap 56.4276°N JP, Nara: Nara c., Naka-machi, Kinki-Uni Nara-Campus 34.671°N TH, Nakhon Nayok: Khao Yai NP (secondary dry evergreen behind football field). Mal14.413°N CH, Kt. Bern, Rüschegg, Eywald 46.75°N JP, Hyougo: Takarazuka C., Kizukinomori 34.844°N