Introduction Why Did England Wish to Establish Colonies?
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Colonial Tunicates: Species Guide
SPECIES IN DEPTH Colonial Tunicates Colonial Tunicates Tunicates are small marine filter feeder animals that have an inhalant siphon, which takes in water, and an exhalant siphon that expels water once it has trapped food particles. Tunicates get their name from the tough, nonliving tunic formed from a cellulose-like material of carbohydrates and proteins that surrounds their bodies. Their other name, sea squirts, comes from the fact that many species will shoot LambertGretchen water out of their bodies when disturbed. Massively lobate colony of Didemnum sp. A growing on a rope in Sausalito, in San Francisco Bay. A colony of tunicates is comprised of many tiny sea squirts called zooids. These INVASIVE SEA SQUIRTS individuals are arranged in groups called systems, which form interconnected Star sea squirts (Botryllus schlosseri) are so named because colonies. Systems of these filter feeders the systems arrange themselves in a star. Zooids are shaped share a common area for expelling water like ovals or teardrops and then group together in small instead of having individual excurrent circles of about 20 individuals. This species occurs in a wide siphons. Individuals and systems are all variety of colors: orange, yellow, red, white, purple, grayish encased in a matrix that is often clear and green, or black. The larvae each have eight papillae, or fleshy full of blood vessels. All ascidian tunicates projections that help them attach to a substrate. have a tadpole-like larva that swims for Chain sea squirts (Botryloides violaceus) have elongated, less than a day before attaching itself to circular systems. Each system can have dozens of zooids. -
Biology Chapter 19 Kingdom Protista Domain Eukarya Description Kingdom Protista Is the Most Diverse of All the Kingdoms
Biology Chapter 19 Kingdom Protista Domain Eukarya Description Kingdom Protista is the most diverse of all the kingdoms. Protists are eukaryotes that are not animals, plants, or fungi. Some unicellular, some multicellular. Some autotrophs, some heterotrophs. Some with cell walls, some without. Didinium protist devouring a Paramecium protist that is longer than it is! Read about it on p. 573! Where Do They Live? • Because of their diversity, we find protists in almost every habitat where there is water or at least moisture! Common Examples • Ameba • Algae • Paramecia • Water molds • Slime molds • Kelp (Sea weed) Classified By: (DON’T WRITE THIS DOWN YET!!! • Mode of nutrition • Cell walls present or not • Unicellular or multicellular Protists can be placed in 3 groups: animal-like, plantlike, or funguslike. Didinium, is a specialist, only feeding on Paramecia. They roll into a ball and form cysts when there is are no Paramecia to eat. Paramecia, on the other hand are generalists in their feeding habits. Mode of Nutrition Depends on type of protist (see Groups) Main Groups How they Help man How they Hurt man Ecosystem Roles KEY CONCEPT Animal-like protists = PROTOZOA, are single- celled heterotrophs that can move. Oxytricha Reproduce How? • Animal like • Unicellular – by asexual reproduction – Paramecium – does conjugation to exchange genetic material Animal-like protists Classified by how they move. macronucleus contractile vacuole food vacuole oral groove micronucleus cilia • Protozoa with flagella are zooflagellates. – flagella help zooflagellates swim – more than 2000 zooflagellates • Some protists move with pseudopods = “false feet”. – change shape as they move –Ex. amoebas • Some protists move with pseudopods. -
Protist Review.Pdf
Protists Termite mound Section 1 Introduction to Protists -!). )DEA Protists form a diverse group of organisms that are subdivided based on their method of obtaining nutrition. Termite colony Section 2 Protozoans— Animal-like Protists -!). )DEA Protozoans are animal-like, heterotrophic protists. Section 3 Algae—Plantlike Protists -!). )DEA Algae are plantlike, autotrophic protists that are the producers for aquatic ecosystems. Section 4 Termites Funguslike Protists SEM Magnification: 17؋ -!). )DEA Funguslike protists obtain their nutrition by absorbing nutrients from dead or decaying organisms. BioFacts • A protist that lives symbiotically Protists in termite gut in the gut of termites helps it LM Magnification: 65؋ digest cellulose found in wood. • The amoeba Amoeba proteus is so small that it can survive in the film of water surrounding particles of soil. • An estimated five million protists can live in one teaspoon of soil. 540 (t)Oliver Meckes/Nicole Ottawa/Photo Researchers, (c)Gerald and Buff Corsi/Visuals Unlimited, (b)Michael Abbey/Photo Researchers , (bkgd)Gerald and Buff Corsi/Visuals Unlimited Start-Up Activities Classify Protists Make this LAUNCH Lab Foldable to help you organize the characteristics of protists. What is a protist? The Kingdom Protista is similar to a drawer or closet in which you keep odds and ends that do not seem to fit any other place. The Kingdom Protista is composed of three groups of organisms that do not fit in any STEP 1 Fold a sheet of notebook paper other kingdom. In this lab, you will observe the three in half vertically. Fold the sheet into thirds. groups of protists. Procedure 1. -
FOSSIL Project Newsletter Summer 2018
News from the FOSSIL Project Vol. 5, Issue 2, Summer 2018 [email protected] www.myfossil.org @projectfossil The FossilProject Club Corner: Dinosaur Research Institute -Discovering the Past With Funds for Future Scholars Inside this issue: Featured Professional: David Bohaska Amateur Spotlight: Nathan Newell Research: Calaveras Dam Featured Fossil: Triceratops vs Tyrannosaurus Education: Wenas Mammoth Foundation Awards DRI has contributed multi-year funding to excavation of the important Albertosaurus Citizen Science at Belgrade bonebed at Dry Island Buffalo Jump Provincial Park where 22 individual Albertosaurus have been identified. FOSSIL Project Updates By Guy McLaughlin Upcoming Events Following the retreat of the last Ice Age 12,000 years ago the meltwater gouged deep, steep-sloped river courses and threaded the Canadian west with coulees, ravines and gullies. Over the millennia their eroding slopes have unveiled a treasure of marine fossils and dinosaur bones revealing the life that flourished in a humid lush climate many millions of years ago. Alberta has an exceptional variety of dinosaur remains and a special responsibility in the study and preservation of this unique resource. Significant funding is required to prospect, excavate, prepare and study this resource before the fossils are exposed to weathering and destruction. Young scientists embarking on this fascinating profession need financial support for their research. The Dinosaur Research Institute (DRI) http://www.dinosaurresearch.com/ was established in 1996 by several keen amateur and professional paleontologists as a non-profit society. Its purpose is to raise and provide financial support for dinosaur research by graduate students and scientists. The Institute funds high-quality scientific dinosaur research on western Canadian dinosaurs; including exploration and recovery, preparation, presentation, and any related research. -
Student Guide Version 20210525 TABLE of CONTENTS
FOUNDATIONS OF BIOTECH COLONY PCR Student Guide www.amgenbiotechexperience.com Version 20210525 TABLE OF CONTENTS Introduction 3 What Is PCR? 4 Laboratory 5-EXT: Using PCR to Amplify the rfp Gene 8 Part A: Performing PCR 9 Part B: Separating PCR Products Using Gel Electrophoresis 12 Chapter 5-EXT Questions 15 Chapter 5-EXT Glossary 16 AMGEN BIOTECH EXPERIENCE | STUDENT GUIDE 1 © 1991, 2000, 2013–2019 Amgen Foundation, Inc. All rights reserved. 2 AMGEN BIOTECH EXPERIENCE—COLONY PCR | STUDENT GUIDE © 1991, 2000, 2013–2019 Amgen Foundation, Inc. All rights reserved. INTRODUCTION One of the most widely used techniques in biotechnology is polymerase chain reaction (PCR). PCR is like a molecular copy machine—it is used to make many (often several billion!) copies of genetic material. PCR was first developed in 1983 by Kary Mullis, an American biochemist who later received the Nobel Prize for his work. PCR has had a profound impact on biotechnology and is now used in many areas of research and applied biotechnology, including genetic engineering, forensics (the use of scientific tests or techniques in crime investigation), and medicine. In this chapter, you will learn about the multiple uses of PCR and then use PCR to determine if the bacterial colonies you created carry the gene of interest, rfp (red fluorescent protein). This type of PCR is referred to as a colony PCR. GOALS By the end of this chapter, you will be able to do the following: • Use PCR to detect the presence of a gene of interest • Describe some of the applications of PCR • Explain the role of enzymes and DNA primers in PCR WHAT DO YOU ALREADY KNOW? Discuss the following questions with your partner, and write your ideas in your notebook. -
Chapter 5. Paleozoic Invertebrate Paleontology of Grand Canyon National Park
Chapter 5. Paleozoic Invertebrate Paleontology of Grand Canyon National Park By Linda Sue Lassiter1, Justin S. Tweet2, Frederick A. Sundberg3, John R. Foster4, and P. J. Bergman5 1Northern Arizona University Department of Biological Sciences Flagstaff, Arizona 2National Park Service 9149 79th Street S. Cottage Grove, Minnesota 55016 3Museum of Northern Arizona Research Associate Flagstaff, Arizona 4Utah Field House of Natural History State Park Museum Vernal, Utah 5Northern Arizona University Flagstaff, Arizona Introduction As impressive as the Grand Canyon is to any observer from the rim, the river, or even from space, these cliffs and slopes are much more than an array of colors above the serpentine majesty of the Colorado River. The erosive forces of the Colorado River and feeder streams took millions of years to carve more than 290 million years of Paleozoic Era rocks. These exposures of Paleozoic Era sediments constitute 85% of the almost 5,000 km2 (1,903 mi2) of the Grand Canyon National Park (GRCA) and reveal important chronologic information on marine paleoecologies of the past. This expanse of both spatial and temporal coverage is unrivaled anywhere else on our planet. While many visitors stand on the rim and peer down into the abyss of the carved canyon depths, few realize that they are also staring at the history of life from almost 520 million years ago (Ma) where the Paleozoic rocks cover the great unconformity (Karlstrom et al. 2018) to 270 Ma at the top (Sorauf and Billingsley 1991). The Paleozoic rocks visible from the South Rim Visitors Center, are mostly from marine and some fluvial sediment deposits (Figure 5-1). -
Cnidaria:Hydrozoa)
Dev Genes Evol (2006) 216:743–754 DOI 10.1007/s00427-006-0101-8 ORIGINAL ARTICLE The evolution of colony-level development in the Siphonophora (Cnidaria:Hydrozoa) Casey W. Dunn & Günter P. Wagner Received: 17 April 2006 /Accepted: 5 July 2006 / Published online: 16 September 2006 # Springer-Verlag 2006 Abstract Evolutionary developmental biology has focused other zooids from a single bud) is a synapomorphy of the almost exclusively on multicellular organisms, but there are Codonophora. The origin of probud subdivision is associated other relevant levels of biological organization that have with the origin of cormidia as integrated units of colony remained largely neglected. Animal colonies are made up organization, and may have allowed for greater morpholog- of multiple physiologically integrated and genetically ical and ecological diversification in the Codonophora identical units called zooids that are each homologous to relative to the Cystonectae. It is also found that symmetry solitary, free-living animals. Siphonophores, a group of is labile in siphonophores, with multiple gains and/or losses pelagic hydrozoans (Cnidaria), have the most complex of directional asymmetry in the group. This descriptive work colony-level organization of all animals. Here the colony- will enable future mechanistic and molecular studies of level development of five siphonophore species, strategi- colony-level development in the siphonophores. cally sampled across the siphonophore phylogeny, is described from specimens collected using deep-sea sub- Keywords Major transition in evolution . mersibles and by self-contained underwater breathing Asexual reproduction . Animal colonies . Division of labor . apparatus diving. These species include three cystonects, Functional specialization Bathyphysa sibogae, Rhizophysa filiformis, and Rhizophysa eysenhardti, and two “physonects”, Agalma elegans and Nanomia bijuga. -
Complex Colony-Level Organization of the Deep-Sea Siphonophore Bargmannia Elongata (Cnidaria, Hydrozoa) Is Directionally Asymmet
DEVELOPMENTAL DYNAMICS 234:835–845, 2005 RESEARCH ARTICLE Complex Colony-Level Organization of the Deep-Sea Siphonophore Bargmannia elongata (Cnidaria, Hydrozoa) Is Directionally Asymmetric and Arises by the Subdivision of Pro-Buds Casey W. Dunn* Siphonophores are free-swimming colonial hydrozoans (Cnidaria) composed of asexually produced multicellular zooids. These zooids, which are homologous to solitary animals, are functionally specialized and arranged in complex species-specific patterns. The coloniality of siphonophores provides an opportunity to study the major transitions in evolution that give rise to new levels of biological organization, but siphonophores are poorly known because they are fragile and live in the open ocean. The organization and development of the deep-sea siphonophore Bargmannia elongata is described here using specimens collected with a remotely operated underwater vehicle. Each bud gives rise to a precise, directionally asymmetric sequence of zooids through a stereotypical series of subdivisions, rather than to a single zooid as in most other hydrozoans. This initial description of development in a deep-sea siphonophore provides an example of how precise colony-level organization can arise, and illustrates that the morphological complexity of cnidarians is greater than is often assumed. Developmental Dynamics 234: 835–845, 2005. © 2005 Wiley-Liss, Inc. Key words: Bargmannia; siphonophore; Hydrozoa; Cnidaria; directional asymmetry; major transitions; deep sea; colonial animal Received 2 March 2005; Revised 2 May 2005; Accepted 5 May 2005 INTRODUCTION which asexually produced individuals colonial taxa show intraspecific vari- remain attached and physiologically ability in zooid arrangement and Most studies of animal development integrated throughout their lives gross colony morphology, such that no have focused on the embryonic devel- (Hughes, 2002). -
Glossary.Pdf
Glossary Pronunciation Key accessory fruit A fruit, or assemblage of fruits, adaptation Inherited characteristic of an organ- Pronounce in which the fleshy parts are derived largely or ism that enhances its survival and reproduc- a- as in ace entirely from tissues other than the ovary. tion in a specific environment. – Glossary Ј Ј a/ah ash acclimatization (uh-klı¯ -muh-tı¯-za -shun) adaptive immunity A vertebrate-specific Physiological adjustment to a change in an defense that is mediated by B lymphocytes ch chose environmental factor. (B cells) and T lymphocytes (T cells). It e¯ meet acetyl CoA Acetyl coenzyme A; the entry com- exhibits specificity, memory, and self-nonself e/eh bet pound for the citric acid cycle in cellular respi- recognition. Also called acquired immunity. g game ration, formed from a fragment of pyruvate adaptive radiation Period of evolutionary change ı¯ ice attached to a coenzyme. in which groups of organisms form many new i hit acetylcholine (asЈ-uh-til-ko–Ј-le¯n) One of the species whose adaptations allow them to fill dif- ks box most common neurotransmitters; functions by ferent ecological roles in their communities. kw quick binding to receptors and altering the perme- addition rule A rule of probability stating that ng song ability of the postsynaptic membrane to specific the probability of any one of two or more mu- o- robe ions, either depolarizing or hyperpolarizing the tually exclusive events occurring can be deter- membrane. mined by adding their individual probabilities. o ox acid A substance that increases the hydrogen ion adenosine triphosphate See ATP (adenosine oy boy concentration of a solution. -
Isolating Microorganisms from Marine and Marine-Associated Samples – a Targeted Search for Novel Natural Antibiotics
Faculty of Natural Resources and Agricultural Sciences Isolating microorganisms from marine and marine-associated samples – A targeted search for novel natural antibiotics Tua Lilja Department of Microbiology Independent project • 15 hec • First cycle, G2E Biotechnology bachelor programme • Examensarbete/Sveriges lantbruksuniversitet, Institutionen för mikrobiologi: 2013:9 • ISSN 1101-8151 Uppsala 2013 Isolating microorganisms from marine and marine-associated samples Tua Lilja Supervisor: Joakim Bjerketorp, Swedish University of Agricultural Sciences, Department of Microbiology Assistant supervisor: Jolanta Levenfors, Swedish University of Agricultural Sciences, Department of Microbiology Examiner: Bengt Guss, Swedish University of Agricultural Sciences, Department of Microbiology Credits: 15 hec Level: First cycle, G2E Course title: Independent project in Biology Course code: EX0689 Programme/education: Biotechnology bachelor programme Place of publication: Uppsala Year of publication: 2013 Title of series: Examensarbete/Sveriges lantbruksuniversitet, Institutionen för mikrobiologi No: 2013:9 ISSN: 1101-8151 Online publication: http://stud.epsilon.slu.se Keywords: antibiotics, natural products, marine microorganisms, isolation methods, actinomycetes, rare actinomycetes, whole-cell screening, antimicrobials, antibiotic resistance Sveriges lantbruksuniversitet Swedish University of Agricultural Sciences Faculty of Natural Resources and Agricultural Sciences Uppsala BioCenter Department of Microbiology ABSTRACT The search for antibiotic -
Bryozoan-Handout.Pdf
1 Bryozoan basics 1 Carl Simpson November 7, 2016 Department of Paleobiology National Museum of Natural History Bryozoans are a phylum of colonial animals. They first appear in the Smithsonian Institution fossil record during the early Ordovician (~480 million years ago.) Washington, D.C. Since that time, bryozoans have been a major component of the fossil record and of marine communities. Their colonies are modular, with [email protected] individual animals, called zooids, forming the building blocks of the http://simpson-carl.github.io colony. There are two primarily marine classes: The Stenolaemata and @simpson_carl the Gymnolaemata. The Phylactolaemata are an exclusively freshwater class. Many fundamental discoveries in evolutionary and paleobiology have been made with bryozoans and many more discoveries await. Figure 1: An SEM micrograph of the bryozoan Schizoporella japonica showing a radial section through a circular Bryozoan zooids colony. The round structures are zooids that are reproductive specialists called ovicells. These ovicells are located in The basic unit of a bryozoan colony is the zooid. Zooids are homol- the center of the colony. Towards the ogous to solitary animals, like a worm or a snail. Within a colony, growing edge (on the right) are the approximately rectangular autozooids zooids depend on each other for survival. If there is a division of with an elliptical orifice where the labor within the colony, then the interdependence between zooids is feeding structure emerges. Proximal even greater. Zooids are small, no more than a 1mm3. The modular to this orifice is a small teardrop- shaped zooid that is a defensive structure of colonies means that they can be of almost any shape and specialist called an avicularium. -
Clonal Colony in the Early Devonian Cnidarian Sphenothallus from Brazil
Clonal colony in the Early Devonian cnidarian Sphenothallus from Brazil HEYO VAN ITEN, JULIANA DE MORAES LEME, MARCELLO G. SIMÕES, and MARIO COURNOYER Van Iten, H., Leme, J.M., Simões, M.G., and Cournoyer, M. 2019. Clonal colony in the Early Devonian cnidarian Sphenothallus from Brazil. Acta Palaeontologica Polonica 64 (2): 409–416. The fossil record of polypoid cnidarians includes a number of taxa that were incorrectly identified as either tubiculous worms or plants. The holotype of the putative alga Euzebiola clarkei (Ponta Grossa Formation, Lower Devonian, Brazil), originally described under the name Serpulites sica, is re-described and re-figured as a species of Sphenothallus, a medu- sozoan cnidarian. Unlike Sphenothallus from other localities, the black, organic-walled Ponta Grossa specimen consists of a single parent tube that is confluent with the apical ends of at least 18 daughter tubes. The pattern of arrangement of the daughter tubes, which are arrayed in single file along the exposed face and the two thickened margins of the parent tube, partly resembles the whorl-like pattern of arrangement of colonial polyps of certain scyphozoan cnidarians. For these reasons, the Ponta Grossa Formation material figures prominently in the argument that Sphenothallus was a me- dusozoan cnidarian capable (in at least one species) of clonal budding. Key words: Cnidaria, Medusozoa, Scyphozoa, Hydrozoa, clonal budding, Devonian, Brazil. Heyo Van Iten [[email protected]], Department of Geology, Hanover College, Hanover, IN 47243, USA and Research Associate, Cincinnati Museum Center, Department of Invertebrate Paleontology, 1301 Western Avenue, Cincinnati, OH 45203, USA. Juliana de Moraes Leme [[email protected]], Department of Sedimentary and Environmental Geology, University of São Paulo, 05508-080, SP, Brazil.