1 2 3 Supplementary Materials for 4 5 Did a plant-herbivore arms race drive chemical diversity in Euphorbia? 6 7 M. Ernst1,2,3, L.-F. Nothias2,3, J. J. J. van der Hooft2,3,4, R. R. Silva2,3, C. H. Saslis-Lagoudakis1, 8 O. M. Grace5, K. Martinez-Swatson1, G. Hassemer1, L. A. Funez7, H. T. Simonsen6, M. H. 9 Medema4, D. Staerk8, N. Nilsson9, P. Lovato9, P. C. Dorrestein2,3,10∗ & N. Rønsted1∗ 10 11 *Correspondence to:
[email protected] and
[email protected] 12 13 14 This PDF file includes: 15 16 Materials and Methods 17 Supplementary Text 18 Table S1 19 Fig S1-S12 20 URL S1 21 Captions for Data S1 and S2 22 23 Other Supplementary Materials for this manuscript include the following: 24 25 Data S1 and S2 26 27 ● List of Euphorbia species sampled. 28 ● High-resolution TNF-α modulation profiles. 29 1 30 Materials and Methods 31 32 Collection of plant material 33 34 Pooled extracts of specimens for each Euphorbia species 35 36 43 Euphorbia species (Data S1) were collected from the greenhouses of the Living Collections 37 of the Botanical Garden in Copenhagen. Live plants were sampled for xerophytic species, 38 whereas herbaceous perennials were grown from seeds originating from the seed bank of the 39 Botanical Garden or collections performed in southern Brazil (Species 11-14 and 16-18). E. 40 myrsinites and E. amygdaloides were purchased as live plants from Jespers Planteskole, 41 Holstebro A/S, Harrestrupvej 64, 7500 Holstebro and Kridtvejs Planter, Kridtvej 18, 7980 42 Vils, Denmark and kept in the greenhouse with the other herbaceous species until harvest.