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ZNF226 Is Over-Expressed in Brain Metastatic Breast Cancer-PDF 111920
1 ZNF226 is over-expressed in brain metastatic breast cancer. 2 Shahan Mamoor East Islip, NY USA1 3 [email protected] 4 5 Metastasis to the brain is a clinical problem in patients with breast cancer1-3. We mined published 6 microarray data4,5 to compare primary and metastatic tumor transcriptomes to discover genes associated with brain metastasis in patients with metastatic breast cancer. We found that the zinc finger protein 226, 7 encoded by ZNF226 was among the genes whose expression was most different in the brain metastases of patients with brain metastatic breast cancer as compared to primary tumors of the breast. ZNF226 may be 8 relevant to processes underlying metastasis of primary tumor-derived cancer cells to the brain in humans 9 with metastatic breast cancer. 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Keywords: breast cancer, brain metastases, central nervous system metastases, ZNF226, systems biology 26 of breast cancer, targeted therapeutics in breast cancer. 27 28 PAGE 1 1 One report described a 34% incidence of central nervous system metastases in trastuzumab- treated patients with breast cancer2. This alarmingly high frequency of CNS metastasis events demands 2 an enhanced understanding of the transcriptional makeup of brain metastatic tissues to support identification of therapeutic targets, whether they are treatment related or not. We performed a global 3 comparative analysis of primary and metastatic tumors in patients with brain metastatic breast cancer4,5. 4 We discovered significant differential and increased expression of ZNF226 in brain metastatic tissues of patients with metastatic breast cancer. -
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PROBING THE INTERACTION OF ASPERGILLUS FUMIGATUS CONIDIA AND HUMAN AIRWAY EPITHELIAL CELLS BY TRANSCRIPTIONAL PROFILING IN BOTH SPECIES by POL GOMEZ B.Sc., The University of British Columbia, 2002 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE STUDIES (Experimental Medicine) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) January 2010 © Pol Gomez, 2010 ABSTRACT The cells of the airway epithelium play critical roles in host defense to inhaled irritants, and in asthma pathogenesis. These cells are constantly exposed to environmental factors, including the conidia of the ubiquitous mould Aspergillus fumigatus, which are small enough to reach the alveoli. A. fumigatus is associated with a spectrum of diseases ranging from asthma and allergic bronchopulmonary aspergillosis to aspergilloma and invasive aspergillosis. Airway epithelial cells have been shown to internalize A. fumigatus conidia in vitro, but the implications of this process for pathogenesis remain unclear. We have developed a cell culture model for this interaction using the human bronchial epithelium cell line 16HBE and a transgenic A. fumigatus strain expressing green fluorescent protein (GFP). Immunofluorescent staining and nystatin protection assays indicated that cells internalized upwards of 50% of bound conidia. Using fluorescence-activated cell sorting (FACS), cells directly interacting with conidia and cells not associated with any conidia were sorted into separate samples, with an overall accuracy of 75%. Genome-wide transcriptional profiling using microarrays revealed significant responses of 16HBE cells and conidia to each other. Significant changes in gene expression were identified between cells and conidia incubated alone versus together, as well as between GFP positive and negative sorted cells. -
Supplementary Table S4. FGA Co-Expressed Gene List in LUAD
Supplementary Table S4. FGA co-expressed gene list in LUAD tumors Symbol R Locus Description FGG 0.919 4q28 fibrinogen gamma chain FGL1 0.635 8p22 fibrinogen-like 1 SLC7A2 0.536 8p22 solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 DUSP4 0.521 8p12-p11 dual specificity phosphatase 4 HAL 0.51 12q22-q24.1histidine ammonia-lyase PDE4D 0.499 5q12 phosphodiesterase 4D, cAMP-specific FURIN 0.497 15q26.1 furin (paired basic amino acid cleaving enzyme) CPS1 0.49 2q35 carbamoyl-phosphate synthase 1, mitochondrial TESC 0.478 12q24.22 tescalcin INHA 0.465 2q35 inhibin, alpha S100P 0.461 4p16 S100 calcium binding protein P VPS37A 0.447 8p22 vacuolar protein sorting 37 homolog A (S. cerevisiae) SLC16A14 0.447 2q36.3 solute carrier family 16, member 14 PPARGC1A 0.443 4p15.1 peroxisome proliferator-activated receptor gamma, coactivator 1 alpha SIK1 0.435 21q22.3 salt-inducible kinase 1 IRS2 0.434 13q34 insulin receptor substrate 2 RND1 0.433 12q12 Rho family GTPase 1 HGD 0.433 3q13.33 homogentisate 1,2-dioxygenase PTP4A1 0.432 6q12 protein tyrosine phosphatase type IVA, member 1 C8orf4 0.428 8p11.2 chromosome 8 open reading frame 4 DDC 0.427 7p12.2 dopa decarboxylase (aromatic L-amino acid decarboxylase) TACC2 0.427 10q26 transforming, acidic coiled-coil containing protein 2 MUC13 0.422 3q21.2 mucin 13, cell surface associated C5 0.412 9q33-q34 complement component 5 NR4A2 0.412 2q22-q23 nuclear receptor subfamily 4, group A, member 2 EYS 0.411 6q12 eyes shut homolog (Drosophila) GPX2 0.406 14q24.1 glutathione peroxidase -
Sumoylation of Heterogeneous Nuclear Ribonucleoproteins, Zinc Finger Proteins, and Nuclear Pore Complex Proteins: a Proteomic Analysis
Sumoylation of heterogeneous nuclear ribonucleoproteins, zinc finger proteins, and nuclear pore complex proteins: A proteomic analysis Tianwei Li*†, Evgenij Evdokimov†‡, Rong-Fong Shen§, Chien-Chung Chao¶, Ephrem Tekle*, Tao Wang‡, Earl R. Stadtman*, David C. H. Yang‡ʈ, and P. Boon Chock*ʈ *Laboratory of Biochemistry and §Proteomic Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8012; ‡Department of Chemistry, Georgetown University, Washington, DC 20057; and ¶Rickettsial Diseases Department͞Infectious Disease Directorate, Naval Medical Research Center, Silver Spring, MD 20910 Contributed by Earl R. Stadtman, April 23, 2004 SUMO, a small ubiquitin-related modifier, is known to covalently SUMO to expose the C-terminal glycine residue; (ii) the het- attach to a number of nuclear regulatory proteins such as p53, IB, erodimeric SUMO-activating enzyme that separates the adeny- promyelocytic leukemia protein and c-Jun. The sumoylation reac- lation and thio-esterification functions into the SAE1 (Aos1 in tion is catalyzed by the SUMO protease, which exposes the C- yeast) and the SAE2 (Uba2 in yeast) subunits, respectively; (iii) terminal active glycine residue of the nascent SUMO, the het- Ubc9, a SUMO-conjugating enzyme that ligates directly to its erodimeric SUMO activating enzyme, the SUMO conjugating protein substrate, such as RanGAP1; and (iv) an E3-like SUMO enzyme, Ubc9, and SUMO protein ligases, in a manner similar to ligase (10, 11). In contrast to the large number of ubiquitin ubiquitinylation. Identification of SUMO-regulated proteins is ligases that have been found, three SUMO E3s have been hampered by the fact that many sumoylated proteins are present identified so far. -
Common Differentially Expressed Genes and Pathways Correlating Both Coronary Artery Disease and Atrial Fibrillation
EXCLI Journal 2021;20:126-141– ISSN 1611-2156 Received: December 08, 2020, accepted: January 11, 2021, published: January 18, 2021 Supplementary material to: Original article: COMMON DIFFERENTIALLY EXPRESSED GENES AND PATHWAYS CORRELATING BOTH CORONARY ARTERY DISEASE AND ATRIAL FIBRILLATION Youjing Zheng, Jia-Qiang He* Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061, USA * Corresponding author: Jia-Qiang He, Department of Biomedical Sciences and Pathobiology, Virginia Tech, Phase II, Room 252B, Blacksburg, VA 24061, USA. Tel: 1-540-231-2032. E-mail: [email protected] https://orcid.org/0000-0002-4825-7046 Youjing Zheng https://orcid.org/0000-0002-0640-5960 Jia-Qiang He http://dx.doi.org/10.17179/excli2020-3262 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). Supplemental Table 1: Abbreviations used in the paper Abbreviation Full name ABCA5 ATP binding cassette subfamily A member 5 ABCB6 ATP binding cassette subfamily B member 6 (Langereis blood group) ABCB9 ATP binding cassette subfamily B member 9 ABCC10 ATP binding cassette subfamily C member 10 ABCC13 ATP binding cassette subfamily C member 13 (pseudogene) ABCC5 ATP binding cassette subfamily C member 5 ABCD3 ATP binding cassette subfamily D member 3 ABCE1 ATP binding cassette subfamily E member 1 ABCG1 ATP binding cassette subfamily G member 1 ABCG4 ATP binding cassette subfamily G member 4 ABHD18 Abhydrolase domain -
Human Induced Pluripotent Stem Cell–Derived Podocytes Mature Into Vascularized Glomeruli Upon Experimental Transplantation
BASIC RESEARCH www.jasn.org Human Induced Pluripotent Stem Cell–Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation † Sazia Sharmin,* Atsuhiro Taguchi,* Yusuke Kaku,* Yasuhiro Yoshimura,* Tomoko Ohmori,* ‡ † ‡ Tetsushi Sakuma, Masashi Mukoyama, Takashi Yamamoto, Hidetake Kurihara,§ and | Ryuichi Nishinakamura* *Department of Kidney Development, Institute of Molecular Embryology and Genetics, and †Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan; ‡Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan; §Division of Anatomy, Juntendo University School of Medicine, Tokyo, Japan; and |Japan Science and Technology Agency, CREST, Kumamoto, Japan ABSTRACT Glomerular podocytes express proteins, such as nephrin, that constitute the slit diaphragm, thereby contributing to the filtration process in the kidney. Glomerular development has been analyzed mainly in mice, whereas analysis of human kidney development has been minimal because of limited access to embryonic kidneys. We previously reported the induction of three-dimensional primordial glomeruli from human induced pluripotent stem (iPS) cells. Here, using transcription activator–like effector nuclease-mediated homologous recombination, we generated human iPS cell lines that express green fluorescent protein (GFP) in the NPHS1 locus, which encodes nephrin, and we show that GFP expression facilitated accurate visualization of nephrin-positive podocyte formation in -
Forms of Supplemental Selenium in Vitamin-Mineral
University of Kentucky UKnowledge Theses and Dissertations--Animal and Food Sciences Animal and Food Sciences 2019 FORMS OF SUPPLEMENTAL SELENIUM IN VITAMIN-MINERAL MIXES DIFFERENTIALLY AFFECT SEROLOGICAL AND HEPATIC PARAMETERS OF GROWING BEEF STEERS GRAZING ENDOPHYTE-INFECTED TALL FESCUE Yang Jia University of Kentucky, [email protected] Digital Object Identifier: https://doi.org/10.13023/etd.2019.028 Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Jia, Yang, "FORMS OF SUPPLEMENTAL SELENIUM IN VITAMIN-MINERAL MIXES DIFFERENTIALLY AFFECT SEROLOGICAL AND HEPATIC PARAMETERS OF GROWING BEEF STEERS GRAZING ENDOPHYTE- INFECTED TALL FESCUE" (2019). Theses and Dissertations--Animal and Food Sciences. 97. https://uknowledge.uky.edu/animalsci_etds/97 This Doctoral Dissertation is brought to you for free and open access by the Animal and Food Sciences at UKnowledge. It has been accepted for inclusion in Theses and Dissertations--Animal and Food Sciences by an authorized administrator of UKnowledge. For more information, please contact [email protected]. STUDENT AGREEMENT: I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained needed written permission statement(s) from the owner(s) of each third-party copyrighted matter to be included in my work, allowing electronic distribution (if such use is not permitted by the fair use doctrine) which will be submitted to UKnowledge as Additional File. I hereby grant to The University of Kentucky and its agents the irrevocable, non-exclusive, and royalty-free license to archive and make accessible my work in whole or in part in all forms of media, now or hereafter known. -
Directed Differentiation of Human Embryonic Stem Cells Into Haematopoietic and Definitive Endodermal Lineages
DIRECTED DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO HAEMATOPOIETIC AND DEFINITIVE ENDODERMAL LINEAGES ABRAHAM SUMAN MARY (M.Sc MICROBIOLOGY, UNIV. OF MUMBAI, INDIA) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOCHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE 2009 ACKNOWLEDGEMENTS In all things I give YOU glory! You have always led me through amazing paths and given me gifts that I don’t deserve. I thank you Lord for all the blessings you constantly shower on me. Everything is possible with God! I thank Dr. Alan Colman for being my guide and helping me to initiate the work contained in this dissertation. His support and encouragement have been invaluable. Thank you, Alan for your support through the years. Dr. Norris Ray Dunn took me under his wing and guided me through this endeavour. He has been a true mentor, always willing to teach, and I have learned a lot from him. Ray, thank you for showing me the way and helping me reach this juncture. A significant part of the research was conducted at ES Cell International Pte Ltd to whom I would like to express my sincere gratitude. Triona, Jacqui, Robert, Michael, Bruce, Chirag, Suzan and so many others have played important roles and encouraged me at all times. Critical portions of this work were done at the Institute of Medical Biology, A*Star. I would like to register my appreciation for the support and help provided by many people in IMB especially, members of the Ray Dunn lab, Mike Jones lab and Alan Colman lab. Kee Yew, thank you for giving me your precious time and helping me with some of the most important data in this dissertation. -
WO 2013/184908 A2 12 December 2013 (12.12.2013) P O P C T
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization I International Bureau (10) International Publication Number (43) International Publication Date WO 2013/184908 A2 12 December 2013 (12.12.2013) P O P C T (51) International Patent Classification: Jr.; One Procter & Gamble Plaza, Cincinnati, Ohio 45202 G06F 19/00 (201 1.01) (US). HOWARD, Brian, Wilson; One Procter & Gamble Plaza, Cincinnati, Ohio 45202 (US). (21) International Application Number: PCT/US20 13/044497 (74) Agents: GUFFEY, Timothy, B. et al; c/o The Procter & Gamble Company, Global Patent Services, 299 East 6th (22) Date: International Filing Street, Sycamore Building, 4th Floor, Cincinnati, Ohio 6 June 2013 (06.06.2013) 45202 (US). (25) Filing Language: English (81) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (30) Priority Data: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, 61/656,218 6 June 2012 (06.06.2012) US DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (71) Applicant: THE PROCTER & GAMBLE COMPANY HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, [US/US]; One Procter & Gamble Plaza, Cincinnati, Ohio KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, 45202 (US). MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, (72) Inventors: XU, Jun; One Procter & Gamble Plaza, Cincin SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, nati, Ohio 45202 (US). -
Genetic Studies of Elite Athlete Status
Wang, Guan (2013) Genetic studies of elite athlete status. PhD thesis. http://theses.gla.ac.uk/4594/ Copyright and moral rights for this work are retained by the author A copy can be downloaded for personal non-commercial research or study, without prior permission or charge This work cannot be reproduced or quoted extensively from without first obtaining permission in writing from the author The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the author When referring to this work, full bibliographic details including the author, title, awarding institution and date of the thesis must be given Enlighten: Theses https://theses.gla.ac.uk/ [email protected] Genetic Studies of Elite Athlete Status by Guan Wang, M.Res. A Doctoral Thesis Submitted in fulfilment of the requirements for the Degree of Doctor of Philosophy August 2013 Institute of Cardiovascular and Medical Sciences College of Medical, Veterinary, and Life Sciences University of Glasgow © G. Wang 2013 2 Author’s Declaration I declare that this thesis is the result of my own work, except that subject recruitment in each country was orchestrated by the relevant PIs as follows: Dr. Yannis Pitsiladis (Jamaican sprint cohort), Dr. Krista Austin (USA sprint cohort), Dr. Rob Lee (Caucasian swim cohort), Dr. Noriyuki Fuku (Japanese swim and track-and-field athlete cohorts), and Dr. Sandy Hsieh (Taiwanese swim cohort); genotyping of Japanese and Taiwanese swim cohort was conducted by Dr. Eri Mikami and Dr. Li-Ling Chiu; genome-wide genotyping of Jamaican and USA sprint cohorts as well as Japanese track-and-field athlete cohort was overseen by Dr. -
Multiple Human Adipocyte Subtypes and Mechanisms of Their Development.”
bioRxiv preprint doi: https://doi.org/10.1101/537464; this version posted January 31, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. “Multiple human adipocyte subtypes and mechanisms of their development.” So Yun Min1,2, Anand Desai1, Zinger Yang2, Agastya Sharma1, Ryan M.J. Genga1,2,4, Alper Kucukural3, Lawrence Lifshitz1, René Maehr1,4, Manuel Garber1,3, Silvia Corvera1,*. 1Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA 2Graduate School oF Biomedical Sciences, University of Massachusetts Medical School, Worcester, MA 01655, USA 3Program in BioinFormatics, University of Massachusetts Medical School, Worcester, MA 01655, USA 4Department of Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01655, USA *Corresponding author: [email protected] KEY WORDS: ADSC, mesenchymal stem cells, pre-adipocyte, adipocyte diFFerentiation, adipocyte development, leptin, adiponectin, iron, brown adipocyte, beige adipocyte, thermogenic Fat, obesity, diabetes SUMMARY Human adipose tissue depots perform numerous diverse physiological functions, and are diFFerentially linked to metabolic disease risk, yet only two major human adipocyte subtypes have been described, white and “brown/brite/beige.” The diversity and lineages oF adipocyte classes have been studied in mice using genetic methods that cannot be applied in humans. Here we circumvent this problem by studying the Fate oF single mesenchymal progenitor cells obtained From human adipose tissue. We report that a minimum oF Four human adipocyte subtypes can be distinguished by transcriptomic analysis, specialized for functionally distinct processes such as adipokine secretion and thermogenesis. -
Identification of Biomarkers, Pathways and Potential Therapeutic Targets for Heart Failure Using Bioinformatics Analysis
bioRxiv preprint doi: https://doi.org/10.1101/2021.08.05.455244; this version posted August 6, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Identification of biomarkers, pathways and potential therapeutic targets for heart failure using bioinformatics analysis Basavaraj Vastrad1, Chanabasayya Vastrad*2 1. Department of Biochemistry, Basaveshwar College of Pharmacy, Gadag, Karnataka 582103, India. 2. Biostatistics and Bioinformatics, Chanabasava Nilaya, Bharthinagar, Dharwad 580001, Karnataka, India. * Chanabasayya Vastrad [email protected] Ph: +919480073398 Chanabasava Nilaya, Bharthinagar, Dharwad 580001 , Karanataka, India bioRxiv preprint doi: https://doi.org/10.1101/2021.08.05.455244; this version posted August 6, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Heart failure (HF) is a complex cardiovascular diseases associated with high mortality. To discover key molecular changes in HF, we analyzed next-generation sequencing (NGS) data of HF. In this investigation, differentially expressed genes (DEGs) were analyzed using limma in R package from GSE161472 of the Gene Expression Omnibus (GEO). Then, gene enrichment analysis, protein-protein interaction (PPI) network, miRNA-hub gene regulatory network and TF-hub gene regulatory network construction, and topological analysis were performed on the DEGs by the Gene Ontology (GO), REACTOME pathway, STRING, HiPPIE, miRNet, NetworkAnalyst and Cytoscape. Finally, we performed receiver operating characteristic curve (ROC) analysis of hub genes. A total of 930 DEGs 9464 up regulated genes and 466 down regulated genes) were identified in HF.