Catalog # Aliquot Size

P14-911B-05 3 x 5 nmol P14-911B-20 3 x 20 nmol P14-911B-50 3 x 50 nmol

PP1B siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # P14-911B

Lot # Z2073-99

Specificity Formulation PP1B siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PP1B expression. shipped at room temperature.

Product Description Reconstitution Protocol PP1B siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PP1B mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_002709. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot PP-1B, PPP1CB, PPP1CD, MGC3672 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PP1A, Active P13-20G below -20oC. After resuspension, siRNA stock solutions ≥2 PP1B, Active P14-20G µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background PP1B or phosphatase 1, catalytic subunit, beta isozyme is one of the three catalytic subunits of 1 (PP1). PPIB is a /threonine specific PP1B siRNA Set I protein phosphatase known to be involved in the siRNA duplexes targeted against three exon regions regulation of a variety of cellular processes, such as , glycogen metabolism, muscle contractility, Catalog # P14-911B protein synthesis, and HIV-1 viral transcription (1). PP1B is Lot # Z2073-99 also known as PP1-delta. PP1B has been reported to Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol functions as a suppressor of learning and memory (2). Format Lyophilized powder Stability 1yr at -70oC from date of shipment Storage & Shipping The lyophilized powder is stable for at References least 4 weeks at room temperature. It is 1. Barker, H. M. et.al: Three for protein recommended that the lyophilized phosphatase 1 map to different human and resuspended siRNAs are stored at o : sequence, expression and gene or below -20 C. After resuspension, siRNA stock solutions ≥2 µM can localisation of protein serine/threonine phosphatase undergo up to 50 freeze-thaw cycles 1 beta (PPP1CB). Biochim. Biophys. Acta 1220: 212- without significant degradation. For 218, 1994. long-term storage, it is recommended that the siRNA is stored at -70oC. For 2. Saadat, M. et.al: Chromosomal localization of most favorable performance, avoid human, rat, and mouse protein phosphatase type 1 repeated handling and multiple beta catalytic subunit genes (PPP1CB) by freeze/thaw cycles. fluorescence in situ hybridization. Jpn. J. Genet. 69: 697-700, 1994.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.