ETUDE CHIMIQUE, ACTIVITES ANTIPLASMODIALE ET ANTIOXYDANTE DE Mussaenda Erectiloba Wernham (Rubiacées)

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ETUDE CHIMIQUE, ACTIVITES ANTIPLASMODIALE ET ANTIOXYDANTE DE Mussaenda Erectiloba Wernham (Rubiacées) UNIVERSITE D’ANTANANARIVO FACULTE DES SCIENCES DOMAINE SCIENCES ET TECHNOLOGIES Mention CHIMIE Mémoire d’étude pour l’obtention du DIPLOME DE MASTER II EN CHIMIE Parcours : CHIMIE DES SUBSTANCES NATURELLES ETUDE CHIMIQUE, ACTIVITES ANTIPLASMODIALE ET ANTIOXYDANTE DE Mussaenda erectiloba Wernham (Rubiacées) Présenté par : FELANARISOA Sandra Le 20 Décembre 2017 Président du Jury : Madame Bakolinirina ANDRIAMIHAJA Professeur Titulaire Rapporteurs : Monsieur Andrianambinina A. RAZAKARIVONY Maître de Conférences Madame Vahinalahaja E. RAZAFINTSALAMA Maître de Recherches Examinateurs : Monsieur David RAMANITRAHASIMBOLA Professeur Madame Lovasoa RABESIAKA Maître de Conférences UNIVERSITE D’ANTANANARIVO FACULTE DES SCIENCES DOMAINE SCIENCES ET TECHNOLOGIES Mention CHIMIE Mémoire d’étude pour l’obtention du DIPLOME DE MASTER II EN CHIMIE Parcours : CHIMIE DES SUBSTANCES NATURELLES ETUDE CHIMIQUE, ACTIVITES ANTIPLASMODIALE ET ANTIOXYDANTE DE Mussaenda erectiloba Wernham (Rubiacées) Présenté par : FELANARISOA Sandra Le 20 Décembre 2017 Président du Jury : Madame Bakolinirina ANDRIAMIHAJA Professeur Titulaire Rapporteurs : Monsieur Andrianambinina A. RAZAKARIVONY Maître de Conférences Madame Vahinalahaja E. RAZAFINTSALAMA Maître de Recherches Examinateurs : Monsieur David RAMANITRAHASIMBOLA Professeur Madame Lovasoa RABESIAKA Maître de Conférences Remerciements Ce travail a été réalisé suivant la collaboration du Laboratoire de Chimie Appliquée aux Substances Naturelles (LaCASN) et le Centre National d’Application de Recherches Pharmaceutiques (CNARP). Tout d’abord, nous souhaitons adresser nos plus vifs remerciements pour les membres du Jury : − Madame Bakolinirina ANDRIAMIHAJA Professeur Titulaire à la Faculté des Sciences de l’Université d’Antananarivo, rattachée au Laboratoire de Chimie Appliquée aux Substances Naturelles (LaCASN) Vous nous faîtes honneur d’avoir accepté de présider ce Jury malgré vos multiples occupations. Nous tenons à vous exprimer notre profonde gratitude et notre sincère reconnaissance pour l’enseignement et la formation que vous nous avez dispensés, pour l’accueil chaleureux, pour la confiance et les soutiens que vous nous avez accordés dans votre laboratoire, pour les conseils, les remarques constructives, les encouragements et la bienveillance dont vous nous avez accompagné tout au long de ce travail. − Monsieur Andrianambinina A. RAZAKARIVONY Maître de Conférences à la Faculté des Sciences de l’Université d’Antananarivo Directeur du Laboratoire de Chimie Appliquée aux Substances Naturelles (LaCASN) Vous avez accepté d’être le Rapporteur de ce mémoire. Nous avons eu le privilège de travailler parmi votre équipe. Nous tenons à vous exprimer notre profonde gratitude et notre sincère reconnaissance pour l’accueil chaleureux dans votre laboratoire, pour les savoir-faire et expériences que vous nous avez partagés, pour votre exigence qui a grandement stimulé nos réflexions, pour vos encouragements et pour votre bienveillance qui nous a permis de travailler dans les meilleures conditions ainsi qu’à votre grande disponibilité malgré vos multiples occupations. − Madame Vahinalahaja E. RAZAFINTSALAMA Maître de Recherches à la Faculté des Sciences de l’Université d’Antananarivo Responsable du Département Pharmacodynamie au Centre National d’Application de Recherches Pharmaceutiques Vous avez accepté d’être le Rapporteur de ce mémoire. Vous nous avez réservé un meilleur accueil malgré vos obligations professionnelles et guidé à chaque étape des études biologiques. Votre participation à l’élaboration de ce mémoire nous a été essentielle. Nous tenons à vous exprimer notre profonde gratitude et notre sincère reconnaissance pour nous avoir initiés aux tests biologiques, pour les compétences et conseils vivement qualitatifs que vous nous avez partagés et pour votre bienveillance qui nous a permis d’arriver au terme de ce travail. − Madame Lovasoa RABESIAKA Maître de Conférences à la Facultés des Sciences de l’Université d’Antananarivo Responsable du Parcours Chimie des Substances Naturelles Vous avez accepté d’être l’Examinateur de ce mémoire. Nous tenons à vous exprimer notre profonde gratitude et notre sincère reconnaissance pour l’enseignement et la formation en Chimie que vous nous avez donnés, pour le temps que vous avez consacré sur ce travail pour le rendre meilleur, vos remarques et vos conseils nous ont été précieux. − Monsieur David RAMANITRAHASIMBOLA Professeur à la Faculté de Médecine de l’Université d’Antananarivo Responsable au Laboratoire de Pharmacologie de l’Institut Malgache de Recherches Appliquées (IMRA) Vous avez accepté avec bienveillance d’être l’Examinateur de ce mémoire. Nous tenons à vous exprimer notre profonde gratitude et notre sincère reconnaissance pour l’enseignement de Pharmacodynamie que vous nous avez donnée en Chimie des Substances Naturelles, pour l’intérêt porté sur ce travail, pour les conseils et les critiques constructives dans le but d’améliorer ce travail. Votre participation à ce travail nous a été précieuse. Notre profonde gratitude et sincère reconnaissance vont aussi à − Madame Reine-Dorothée RAZAFIMAHEFA-RAMILISON Professeur à la Faculté des Sciences de l’Université d’Antanananrivo, rattachée au Laboratoire de Chimie Appliquée aux Substances Naturelles (LaCASN) Nous tenons à vous exprimer notre profonde gratitude et notre sincère reconnaissance pour l’enseignement que vous nous avez dispensés en Chimie, pour l’accueil chaleureux et la confiance dont vous nous avez accordés dans votre laboratoire, pour vos soutiens et votre bienveillance dans la réalisation de ce travail. − Docteur Michel RATSIMBASON Directeur du Centre National d’Application des Recherches Pharmaceutiques (CNARP). Nous tenons à vous exprimer notre profonde gratitude et notre sincère reconnaissance pour l’aimable accueil et la collaboration dont vous nous avez accordés. Ce qui nous a permis de réaliser les tests biologiques au Centre National d’Application de Recherches Pharmaceutiques et d’arriver au terme de ce mémoire. − Une pensé particulière est pour Professeur Bakonirina RAZANAMAHEFA Ancienne Directeur du Laboratoire de Chimie Appliquée aux Substances Naturelles (LaCASN) Elle nous accueillie dans son laboratoire et nous a accordée ses soutiens. Elle nous a quittés bien trop tôt mais elle nous a énormément apprise durant ces quelques années en la côtoyant avec sa gentillesse qui nous a beaucoup marquée. Nous adressons notre sincère et respectueuse reconnaissance − A Monsieur le Doyen de la Faculté des Sciences qui nous a permis d’arriver au terme de ce jour. − A Monsieur Dimby RALAMBOMANANA, Maître de Conférences, Responsable de la Mention Chimie et Directeur du Laboratoire de Chimie Organique « Produits Naturels » et Biotechnologie (LPNB), pour sa collaboration qui nous a permis d’arriver en ce jour. − A tous les professeurs qui ont participé à nos enseignements et formations durant ces années d’étude universitaire. − A la Comité de lecture pour l’intérêt porté et le temps consacré sur ce travail. Nos sincères remerciements vont aussi : − A Monsieur Rivoarison RANDRIANASOLO, Maître de Conférences à la Faculté des Sciences, Responsable du Parcours Chimie Biologie, Membre du Laboratoire de Chimie des Substances Naturelles et Chimie Organique Biologique (LCSN/COB), pour la récolte sur terrain − A Docteur Franck RAKOTONASOLO, botaniste au Parc Botanique et Zoologique de Tsimbazaza, pour l’identification de la plante. − A Docteur Sylvie ANDRIAMBOLOLONERA, Coordinatrice de l’Unité Recherche au sein du Programme Madagascar du Missouri Botanical Garden, pour avoir acceptée avec amabilité de collaborer pour l’étude botanique de la plante malgré ses multiples occupations. − A Monsieur Jesuka R. RASOLOFOMANANA, Membre du Département Pharmacodynamie au Centre National d’Application de Recherches Pharmaceutiques, pour les tests antipaludiques, les conseils et aides précieux qu’il m’a accordée. − A Madame Rojoniaina A. RAKOTOARISON et notre collègue de paillasse Njara pour nous avoir aidés dans les tests antioxydants. − A Monsieur Maonja F. RAKOTONDRAMANGA, Maître de Conférences, pour le transport des produits à l’étranger qui a permis d’obtenir les spectres RMN et pour le moteur de recherche qu’il nous a partagé. − A Monsieur Faly RANDRIAMIALINORO, Maître de Recherches au Centre National d’Application de Recherches Pharmaceutiques, pour les aides qu’il nous a apportés. − A Monsieur Ralinandrianina RANDRIAMIARAMISAINA, Enseignant-chercheur et membre de l’équipe LaCASN pour ses aides pratiques en laboratoire. − A Monsieur Hariliva RAMIARISON, Enseignant-chercheur et membre de l’équipe LaCASN pour les moteurs de recherches. − A Madame N. J. Patricia RAMANANTSOA, membre de l’équipe LaCASN pour ses aides dans la réalisation des diapositives. − A tous les membres de l’équipe du laboratoire de Chimie Appliquée aux Substances Naturelles (LaCASN) pour leurs interventions afin de rendre ce travail meilleur, pour l’ambiance et la convivialité. − A tous les membres du Centre National d’Application de Recherches Pharmaceutiques pour l’aimable accueil, l’ambiance et la convivialité. − A mes collègues de promotions pour leurs aides. Je souhaite remercier mes parents pour la confiance qu’ils m’ont accordée et les soutiens qu’ils m’ont donnés tout au long de mes études. Un grand merci à Ravo, Dina, Herizo et Telina qui par leurs présences m’ont donnée de grande motivation. Je n’oublie pas mes deux grand-mères qui n’ont cessé de m’encourager, merci. Je remercie aussi Ranto pour ses encouragements sans relâches et son soutien. A
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