Зборник Матице српске за природне науке / Matica Srpska J. Nat. Sci. Novi Sad, № 133, 307—314, 2017

UDC 597.8:616.988 https://doi.org/10.2298/ZMSPN1733307S

Miloš Č. STUPAR* , Katarina V. BREKA, Imre I. KRIZMANIĆ, Srđan Z. STAMENKOVIĆ, Nikola D. UNKOVIĆ, Željko D. SAVKOVIĆ, Jelena B. VUKOJEVIĆ, Milica V. LJALJEVIĆ-GRBIĆ

University of Belgrade, Faculty of Biology, Studentski trg 16, Belgrade 11000, Republic of Serbia

FIRST CASE REPORT ON PATHOGENIC Fonsecaea sp. Negroni FROM SKIN OF Pelophylax kl. esculentus L. IN SERBIA

ABSTRACT: Non-harmful adhesive tape method was applied directly on the skin surface of edible frog (Pelophylax kl. esculentus), captured in vernal ponds on the locality “Stevanove ravnice” within the Special Nature Reserve „Deliblatska peščara“, in order to detect fungal dwellers of frogs’ skin. Light microscopy analyses of LactophenolCottonBlue- mounted adhesive tape samples taken from frog’s ventrum revealed the presence of melanized septate hyphae, branched conidiophores with chains of single-celled ovoid conidia, arising directly from the skin, which corresponds to morphological features of dematiaceous hy- phomycete – Fonsecaea sp. Since members of genus Fonsecaea are frequently cited as causative agents of chromomycosis in amphibians, as well as human phaeohyphomycosis, world- wide, it is of great significance to study the presence of this fungal pathogen on amphibians in Serbia in order to make the basic reference data of the incidence of these pathogens in this region. KEYWORDS: adhesive tape method, chromomycosis, dematiaceous fungi, Fonsecaea sp., frogs, pathogen

INTRODUCTION

Since the discovery of amphibian chytrid fungus, Batrachochytrium den­ drobatidis Longcore, Pessier & D.K. Nichols, pathogen responsible for extinction and rapid decline in frogs’ population worldwide (Berger et al. 1999; Woodhams et al. 2012), more attention is given to fungal infections of amphibians, in general. The degree of susceptibility to B. dendrobatidis varies greatly between species and is attributable to multiple factors including host physiology, envi- ronmental conditions and the skin microbial communities (Bletz et al. 2013).

* Corrensponding author. E-email: [email protected] 307 Concerning these factors, skin microbial communities have come to the fore- front of research on B. dendrobatidis susceptibility due to the role symbiotic bacteria play in host resistance. During metamorphosis, the skin of the body becomes increasingly keratinized and the fungal infection is then able to spread over the skin of susceptible species (Marantelli et al. 2004; Rachowicz and Vredenburg, 2004). Apart from B. dendrobatidis, causative agent of chytridyo- mycoses, amphibians (anurans and caudates) are also susceptible to other fun- gal infections such as mucormycoses, basidiobolomycoses, saprolegniases and chromomycoses (Pessier, 2002; Pare, 2003; Densmore and Green, 2007). Darkly pigmented filamentous fungi, i.e. dematiaceous hyphomycetes, members of genera Cladosporium, Exophiala, Fonsecaea, Phialophora, Scolecobasidium and Veronaea, are causative agents of cutaneous and systemic chromoblasto- mycoses of post-metamorphic anurans. This presents chronic, cutaneous and subcutaneous infection, characterized by slowly expanding nodules that even- tually lead to emerging, cauliflower-like, mutilating and disfiguring eruptions. Infection proceeds with muriform cells in tissue provoking a granulomatous immune response (Pare, 2003; Hosoya et al. 2015). Propagules of these fungi (conidia, ascospores, chlamydospores, hyphal fragments) are often present in soil and leaf litter. Seldomly, these saprotrophic fungi act as oportunistic path- ogens (Pare, 2003), infecting anurans through traumatic inoculation of fungal propagules, followed by dissemination to internal organs (Pessier, 2002). Ascomycete Fonsecaea is an anamorph member of the family Herpotrichiel- laceae, order class . This genus contained only three described species, namely F. pedrosoi (including its morphological variant F. compacta), F. monophora and F. nubica (de Hoog et al. 2004). These species are morphologically indistinguishable and could be separated only on the basis of ITS sequencing. Also, Vicente et al. (2012) described and reported novel species in this genus, F. brasiliensis, associated with letargic crab disease of mangrove crab, Ucides cordatus L. Relationships between microbial communities and their hosts can be highly complex. Host-microbiome interactions could be influenced by numerous factors which include host traits such as genetics, life history and behavior, as well as broader effects of environmental factors (Ding and Schloss, 2014). Furthermore, the skin of different amphibian species, and even individuals, may vary by both the presence and the type of anti-microbial peptides, mucosal secretions and levels of skin sloughing, which can affect the formation and maintenance of resident microbiota (Meyer et al. 2012). Current studies are focused on elucidating the basic biology governing the host-microbiome relationship to confidently and ef- fectively implement microbiome science in conservation efforts (Longo et al. 2015). Special Nature Reserve “Deliblatska peščara” is situated in North Serbia and represents the largest European continental sand. Due to flora and fauna species richness, “Deliblatska peščara” is one of the most important centers of biodiversity in Serbia and Europe and is protected by a Decree of the Government of the Republic of Serbia (Official Gazette of RS, no. 3/2002). As the interna- tionally significant bird habitat, it is included in the Ramsar List of Wetlands of International Importance (Josimović and Pucar, 2010). Many rare species

308 nest in the area of the flood riverbanks of the Danube river where there is one of the largest migratory stations of water birds in Serbia as well as a nesting place of many rare and threatened bird species such as Little egret (Egretta garzetta L.), common pochard (Aythya ferina L.), pygmy cormorant (Microcarbo pygmeus Pallas) etc. This is also one of the areas in Serbia that are inhabited with all three taxa of the Pelophylax synklepton esculentus complex (P. lessonae, P. ridibundus and hybrid species P. kl. esculentus). Also, this region represents south- ern limit of distribution for P. lessonae. During their whole life cycle, green frogs represent important food source for water birds and other higher level consumers. The main aim of this research was to get insight into frog’s epidermis-asso- ciated mycobiota and detection of potential fungal pathogens via application of the adhesive tape method, already succesfully applied in medical and veterinary mycology (Harris, 2000), for the first time on anuran skin, which could lead to recognizing and registration of novel fungal pathogens in this region. Also, investigation of specific biological communities within protected areas, could lead to implementation of more effective management strategies.

MATERIALS AND METHODS

Specimen of water frog, Pelophylax synklepton esculentus complex was captured in vernal ponds on the locality “Stevanove ravnice” within the Special Nature Reserve “Deliblatska peščara” in September 2016. Based on qualitative traits and morphometric parameters (Krizmanić, 2008), the captured individual was identified as male specimen of edible frog Pelophylax kl. esculentus L. (Figure 1).

Figure 1. Pelophylax kl. esculentus in its natural habitat (Photo K. Breka)

309 Captured specimen was put in wet denim sack and proceeded to field laboratory. In laboratory conditions, adhesive tape was gently adhered to five different skin areas (dorsal and ventral side, head, fore- and hindlimbs) and removed imperceptibly (Urzı̀ and De Leo, 2001). Adhesive tapes samples were then mounted in standard mycological dye LactophenolCottonBlue (LCB), at- tached to microscope slides and observed under light microscope (Nikon Eclipse E200, equipped with camera Bresser MikroCam PRO HDMI, Japan). Observed and documented fungal structures were compared with avaliable identification keys in order to identify fungi present in the samples (Larone, 1989). After the examination, captured frog specimen was safely returned to its original habitat.

RESULTS AND DISCUSSION

Adhesive type samples taken directly from skin of the ventrum and inves- tigated under light microscopy revealed the presence of well developed mycelium consisted of septate, melanized and loosely branching hyphae, with conidiog- enous apparatus producing an asterisk-like appearance (Figure 2a). Abundant septate conidiophores, erected from somatic hyphae, bearing short chains of single celled ovoid conidia (Ø 3.5–5 x 1.5–2µm) were frequently observed (Figure 2b). Apparently, the mycelial growth and conidiation were abundant on frog’s ventral side and typical conidia with denticles were observed in mass (Figure 2c). These fungal structures were not detected on other parts of frog’s skin. According to Larone (1989) documented micromorphological features, including conidia shape and size, type of conidiation and branching of con- idiophores, correnspond to dematiaceous fungus, Fonsecaea sp. Additionally, morphological identification of documented fungal structures was confirmed via online mycological database presented on the website “Mycology online” (http://www.mycology.adelaide.edu.au/descriptions/hyphomycetes/fonsecaea). Morphologically, four types of conidial formation were described for Fonse­ caea species: Fonsecaea, Rhinocladiella, Cladosporium and Phialophora type and morphological features observed on P. kl. esculentus correnspond to Fon­ secaea type of conidiation (septate and erect conidiophores, primary conidia produced on swollen denticles, long conidial chains not formed). In axenic cultures, Fonsecaea spp. are characterized by slow growing colonies, displaying flat to heaped and folded shape, with suede-like to downy or olivaceous to black coloration with black reverse. The identification of Fon­ secaea spp. to species level based solely on morphological criteria is very dif- ficult, due to polymorphysm, and hence for proper and more detailed identification of these fungi, isolation of pathogen is requiered, followed by additional molecular analyses or metagenomics approach. Although, none of known chromomycoses symptoms (ie. cutaneous lesions, nodules, skin ulcers…) were documented during examination of captured frog specimen, the presence of Fonsecaea sp. structures, in form of well developed mycelial phase and abundant sporulation directly on frogs skin, is very significant, since Fonsecaea spp. is cited as causative 310 Figure 2. Fonsecea sp. growing and sporulating on Pelophylax kl. esculentus skin, dyed with LCB; a) mycelium with asterisk-like conidiogenous apparatus (bar represents 50 μm); b) detail of conidiogenus apparatus (bar represents 10 μm); c) single-celled ovoid conidia with denticles in mass (bar represents 10 μm). agent of chromomycoses of amphibians. Infections caused by F. pedrosoi were reported for cane toad, Rhinella marina L. (Cicmanec et al. 1973) and northern leopard frog Lithobates pipiens Schreber (Rush et al. 1974). Albeit, infections caused by F. pedrosoi and other dematiaceous fungi affect people, via chromo- blastomycosis and phaeohyphomycosis, pathogen transmission from amphibians to humans has not yet been reported (Pare, 2003). Likewise, since the dema- tiaceous hyphomycetes are usually considered as opportunistic or secondary

311 pathogens of anurans, it could be assumed that examined frog specimen also suffer from some other primary fungal or bacterial infection.

CONCLUSION

In this research the application of adhesive tape method directly on am- phibians’ stratum corneum was demonstrated for the first time as useful tool for preliminary observation of fungal skin dwellers. Not only transients but also the potential pathogens could be detected via adhesive tape method. This method is completely safe and provides minimal stress to studied animal, so it can be introduced as significant diagnostic tool for detection of epizootic communities of frogs and other amphibians, possibly cyst and zoosporangium of B. dendro­ batidis, as well. In further researches other amphibian species from different localities in Serbia should be included. This resarch could be helpful in further studies on amphibian declines and their causes as well as on amphibian con- servation, with an emphasis on those that describe methods for monitoring and conserving amphibian populations in Serbia.

ACKNOWLEDGEMENTS

Ministry of Education and Science of the Republic of Serbia project No173032; Rufford project No 19434-1.

REFERENCES

Berger L, Speare R, Hyatt A (1999): Chytrid fungi and amphibian declines: overview, implica- tions and future directions. Declines and disappearances of Australian frogs. Environment Australia, Canberra, 23–33. Bletz MC, Loudon AH, Becker MH, Bell SC, Woodhams DC, Minbiole PK, Harris N (2013): Mitigating amphibian chytridiomycosis with bioaugmentation: characteristics of effective probiotics and strategies for their selection and use. Ecol. Lett. 16: 807–820. Cicmanec JL, Ringler DH, Beneke ES (1973): Spontaneous occurrence and experimental trans- mission of the fungus, , in the marine toad, Bufo marinus. Lab. Anim. Sci. 23: 43. De Hoog GS, Attili-Angelis D, Vicente VA, Gerrits Van Den Ende, AHG, Queiroz-Telles F (2004): Molecular ecology and pathogenic potential of Fonsecaea species. Med. Mycol. 42: 405–416. Densmore CL, Green DE (2007): Diseases of amphibians. ILAR J. 48: 235–254. Ding T, Schloss, PD (2014): Dynamics and associations of microbial community types across the human body. Nature 509: 357–360. Harris JL (2000): Safe, low-distortion tape touch method for fungal slide mounts. J. Clin. Micro­ biol. 38: 4683–4684.

312 Hosoya T, Hanafusa Y, Kudo T, Tamukai K, Une Y (2015): First report of Veronaea botryosa as a causal agent of chromomycosis in frogs. Med. Mycol. 53: 369–377. Josimović B, Pucar M (2010): The strategic environmental impact assessment of electric wind energy plants: Case study ’Bavanište’(Serbia). Renew. Energy 35: 1509–1519. Krizmanić II (2008): Water frogs (Rana esculenta complex) in Serbia: Morphological data. Arch. Biol. Sci. 60: 449–457. Larone DH (1989): The identification of dematiaceous fungi. Clin. Microbiol. Newsl. 11: 145–150. Longo AV, Savage AE, Hewson I, Zamudio, KR (2015): Seasonal and ontogenetic variation of skin microbial communities and relationships to natural disease dynamics in declining amphibians. R. Soc. Open sci. 2: 140377. Marantelli G, Berger L, Speare R, Keegan L (2004): Distribution of the amphibian chytrid Batrachochytrium dendrobatidis and keratin during tadpole development. Pac. Conserv. Biol. 10: 173–179. Meyer EA, Cramp RL, Hernando Bernal M, Franklin CE (2012): Changes in cutaneous microbial abundance with sloughing: possible implications for infection and disease in amphibians. Dis. Aquat. Org. 101: 235–242. Paré JA (2003): Fungal diseases of amphibians: an overview. Vet. Clin. N. Am: Exotic Animal Practice 6: 315–326. Pessier AP (2002): An overview of amphibian skin disease. Semin. Avian Exot. Pet Med. 11, 162–174. Rachowicz LJ, Vredenburg VT (2004): Transmission of Batrachochytrium dendrobatidis within and between amphibian life stages. Dis. Aquat. Org. 61: 75–83. Rush HG, Anver MR, Beneke ES (1974): Systemic chromomycosis in Rana pipiens Lab. Anim. Sci. 24: 646. Urzı̀ C, De Leo F (2001): Sampling with adhesive tape strips: an easy and rapid method to monitor microbial colonization on monument surfaces. J. Microbiol. Methods. 44: 1–11. Vicente VA, Orélis-Ribeiro R, Najafzadeh MJ, Sun J, Schier Guerra R, Meis JF, Ostrensky A, Klaassen CH, De Hoog GS, Miesch S, Boeger WA (2012): Black yeast-like fungi associ- ated with Lethargic Crab Disease (LCD) in the mangrove-land crab, Ucides cordatus (Ocypodidae). Vet. Microbiol. 158: 109–122. Woodhams DC, Bigler L, Marschang R (2012): Tolerance of fungal infection in European water frogs exposed to Batrachochytrium dendrobatidis after experimental reduction of innate immune defenses. BMC Vet. Res. 8: 197.

313 ПРВИ ПРИКАЗ СЛУЧАЈА ПАТОГЕНЕ ГЉИВЕ Fonsecaea sp. Negroni НА КОЖИ Pelophylax kl. esculentus L. У СРБИЈИ Милош Ч. СТУПАР, Катарина В. БРЕКА, Имре И. КРИЗМАНИЋ, Срђан З. СТАМЕНКОВИЋ, Никола Д. УНКОВИЋ, Жељко Д. САВКОВИЋ, Јелена Б. ВУКОЈЕВИЋ, Милица В. ЉАЉЕВИЋ-ГРБИЋ

Универзитет у Београду, Биолошки факултет, Студентски трг 16, Београд 11000, Република Србија

РЕЗИМЕ: У циљу детекције и идентификације фунгалних колонизатора коже зелене жабе (Pelophylax kl. esculentus), из ефемерних бара с локалитета „Стевано­ ве Равнице” у оквиру Специјалног резервата природе „Делиблатска пешчара”, из­вршено је узорковање безбедном методом адхезивне траке. Микроскопска ана­лиза је показала присуство меланизованих, септираних хифа и гранатих кони­ диофора којe се уздижу директно с епидермиса трбушне странe жабе и носе тер­ минално постављене ланце једноћелијских, овалних конидија. Документоване микроморфолошке карактеристике одговарају опису патогене гљиве рода Fon­ s e ­c a e a . Овај налаз је значајан обзиром да се врсте овог рода наводе као узрочници хромомикоза водоземаца, као и хуманих феохифомикоза. Како присуство F o n s e ­c a e a sp. на кожи зелене жабе у Србији није саопштено, од великог је значаја даље про­ уча­вање ове патогене гљиве у циљу сакупљања референтних података о њеној дистрибуцији у региону. КЉУЧНЕ РЕЧИ: метода адхезивне траке, хромомикоза, патогена гљива, Fonsecaea sp., жабе, патоген

314