Table S4. Qpcr Assays Results, Shown As Average Delta Threshold Cycle ( CT) Values For
Table S4. qPCR assays results, shown as average delta threshold cycle (∆CT) values for the four field replicates calculated according to Schmittgen and Livak (1). The CT is the difference of the 16S rRNA CT and each resistance gene CT. A value closer to zero corresponds to higher relative copy number. Forward primer sequences are provided as a signature to compare results between data tables. The first entry, “16S rRNA (CT)”, signifies at this row contains the CT values for the 16S rRNA gene assay which is the reference gene, opposed to ∆CT values as are listed in all other rows.
Gene / BM / BC / BS / JM / JC / JS / PM / PC / PS / Forward Primer16S rRNA (CT) / 8.73 / 10.50 / 11.21 / 8.85 / 10.22 / 11.58 / 8.84 / 9.89 / 11.19 / GGGTTGCGCTCGTTGC
aac / 15.40 / CCCTGCGTTGTGGCTATGT
aac(6')-Ib / 6.23 / 12.35 / 14.90 / 7.23 / 8.11 / 12.19 / 13.23 / 6.86 / 9.57 / CGTCGCCGAGCAACTTG
aac(6')-Ib / 6.20 / 12.88 / 15.09 / 7.10 / 9.37 / 13.28 / 13.62 / 7.32 / 11.71 / GTTTGAGAGGCAAGGTACCGTAA
aac(6')-Ib / 6.31 / 12.23 / 14.18 / 7.08 / 8.87 / 12.18 / 13.60 / 6.45 / 9.01 / AGAAGCACGCCCGACACTT
aac(6')-II / 12.51 / 10.97 / 15.34 / 8.60 / 9.64 / 11.31 / 16.36 / 6.46 / 9.07 / CGACCCGACTCCGAACAA
aacC / 17.20 / 15.01 / 17.30 / 14.49 / 12.13 / CGTCACTTATTCGATGCCCTTAC
aacC1 / 17.38 / 14.60 / 18.06 / 15.91 / GGTCGTGAGTTCGGAGACGTA
aacC2 / 13.31 / 12.30 / 14.46 / 9.34 / 13.17 / 14.99 / 10.93 / 10.90 / 13.30 / ACGGCATTCTCGATTGCTTT
aacC4 / 5.90 / 14.96 / 10.38 / 12.20 / 9.83 / 12.84 / 14.40 / CGGCGTGGGACACGAT
aadA5 / 6.85 / 10.09 / 12.43 / 10.31 / 6.20 / 11.91 / 16.85 / 5.29 / 8.53 / GTTCTTGCTCTTGCTCGCATT
aadA5 / 7.11 / 10.31 / 12.63 / 10.37 / 6.54 / 11.99 / 16.12 / 5.97 / 9.22 / ATCACGATCTTGCGATTTTGCT
aadA / 4.01 / 7.36 / 10.27 / 6.81 / 4.53 / 10.73 / 8.10 / 3.50 / 7.14 / CGAGATTCTCCGCGCTGTA
aadA / 3.75 / 7.18 / 10.14 / 7.05 / 4.76 / 10.01 / 8.07 / 2.96 / 13.35 / GTTGTGCACGACGACATCATT
aadA1 / 3.82 / 7.50 / 10.65 / 7.08 / 5.64 / 10.44 / 8.52 / 3.86 / 6.55 / AGCTAAGCGCGAACTGCAAT
aadA2 / 3.60 / 7.25 / 9.67 / 6.61 / 3.71 / 9.08 / 7.51 / 2.96 / 5.64 / CAATGACATTCTTGCGGGTATC
aadA2 / 4.87 / 9.23 / 11.53 / 8.29 / 4.79 / 8.84 / 9.08 / 4.09 / 6.34 / CTTGTCGTGCATGACGACATC
aadA2 / 4.54 / 9.25 / 10.78 / 8.14 / 4.79 / 9.37 / 8.87 / 4.13 / 11.77 / ACGGCTCCGCAGTGGAT
aadA9 / 6.57 / 10.74 / 13.59 / 11.89 / 6.97 / 12.71 / 7.70 / 12.56 / GGATGCACGCTTGGATGAA
aadA9 / 6.35 / 11.37 / 14.26 / 11.96 / 6.85 / 13.33 / 17.49 / 7.85 / 12.82 / CGCGGCAAGCCTATCTTG
aadD / 6.24 / 13.18 / 4.51 / 10.32 / 14.69 / 8.09 / 8.69 / 13.60 / CCGACAACATTTCTACCATCCTT
aphA3 / 2.35 / 12.77 / 14.46 / 0.89 / 12.00 / 13.83 / 0.58 / 6.24 / 13.31 / CGGAATTGAAAAAACTGATCGAA
aphA3 / 2.81 / 14.31 / 14.71 / 1.57 / 8.38 / 10.97 / 0.37 / 6.23 / 13.79 / AAAAGCCCGAAGAGGAACTTG
aph(2')-Id / 15.05 / TGAGCAGTATCATAAGTTGAGTGAAAAG
aphA1 / 3.67 / 13.67 / 7.30 / 9.30 / 13.20 / 7.99 / 6.62 / 10.31 / TGAACAAGTCTGGAAAGAAATGCA
strA / 8.74 / 15.19 / 12.08 / 10.79 / 13.15 / 10.17 / 12.96 / CCGGTGGCATTTGAGAAAAA
strB / 3.39 / 9.36 / 12.42 / 6.05 / 5.06 / 11.96 / 8.14 / 4.30 / 6.74 / GCTCGGTCGTGAGAACAATCT
bla1 / 15.21 / GCAAGTTGAAGCGAAAGAAAAGA
blaCTX-M / 17.66 / GCGATAACGTGGCGATGAAT
blaCTX-M / 17.12 / 16.97 / 13.79 / 16.88 / GGAGGCGTGACGGCTTTT
blaGES / 17.85 / GCAATGTGCTCAACGTTCAAG
blaOXY / 15.16 / 14.10 / 16.11 / 13.21 / CGTTCAGGCGGCAGGTT
blaPSE / 8.98 / 14.81 / 10.45 / 13.37 / 15.19 / 10.93 / 12.26 / TTGTGACCTATTCCCCTGTAATAGAA
blaROB / 17.39 / 15.17 / 16.51 / GCAAAGGCATGACGATTGC
blaSHV / 17.31 / 15.20 / 16.99 / 14.65 / 15.96 / 14.95 / TCCCATGATGAGCACCTTTAAA
blaTEM / 8.31 / 14.28 / 13.23 / 11.03 / 13.10 / 12.01 / 9.67 / 7.98 / 9.20 / AGCATCTTACGGATGGCATGA
blaTLA / 12.72 / ACACTTTGCCATTGCTGTTTATGT
blaVEB / 14.98 / CCCGATGCAAAGCGTTATG
blaZ / 12.26 / GGAGATAAAGTAACAAATCCAGTTAGATATGA
cfxA / 15.30 / 6.02 / 10.87 / TCATTCCTCGTTCAAGTTTTCAGA
Pbp5 / 18.14 / 17.99 / GGCGAACTTCTAATTAATCCTATCCA
penA / 15.62 / 17.12 / 15.09 / 15.10 / 14.28 / AGACGGTAACGTATAACTTTTTGAAAGA
bla-L1 / 16.10 / 14.78 / 15.56 / 17.90 / 14.68 / 10.76 / 13.63 / 14.72 / CACCGGGTTACCAGCTGAAG
cphA / 15.37 / 17.44 / 16.21 / 14.94 / 15.89 / 14.82 / 14.09 / GCGAGCTGCACAAGCTGAT
ampC / 15.63 / CAGCCGCTGATGAAAAAATATG
ampC / 17.65 / 16.84 / TGGCGTATCGGGTCAATGT
ampC / 18.01 / 16.85 / CTGTTCGAGCTGGGTTCTATAAGTAAA
ampC / 10.18 / 12.90 / 10.84 / 12.01 / AACAAAAGATCCCCGGTATGG
ampC / 10.60 / 14.26 / 12.66 / 11.22 / 12.17 / GCAGCACGCCCCGTAA
ampC / 17.80 / 15.69 / 17.81 / 14.99 / CCGCTCAAGCTGGACCATAC
ampC/ blaDHA / 15.69 / 16.67 / TGGCCGCAGCAGAAAGA
blaCMY2 / 15.44 / GCGAGCAGCCTGAAGCA
blaCMY2 / 17.98 / 15.59 / AAAGCCTCAT GGGTGCATAAA
blaMOX/ blaCMY / 15.36 / 15.35 / CTATGTCAATGTGCCGAAGCA
blaPAO / 18.00 / 13.96 / 10.65 / 14.12 / CGCCGTACAACCGGTGAT
fox5 / 14.82 / 14.91 / GGTTTGCCGCTGCAGTTC
blaOXA1/ blaOXA30 / 16.15 / 12.61 / 15.15 / CGGATGGTTTGAAGGGTTTATTAT
blaOXA10 / 9.36 / 10.85 / 14.66 / 17.91 / 11.75 / 13.22 / CGCAATTATCGGCCTAGAAACT
blaOXA10 / 9.70 / 15.65 / 10.66 / 14.74 / 15.36 / 17.00 / 11.34 / 12.96 / CGCAATTATCGGCCTAGAAACT
cmlA1 / 6.78 / 11.94 / 13.94 / 10.01 / 5.71 / 13.63 / 9.85 / 4.88 / 8.44 / TAGGAAGCATCGGAACGTTGAT
cmlA1 / 12.69 / 11.66 / 15.04 / 9.99 / 6.59 / 14.57 / 9.65 / 5.66 / 8.71 / AGGAAGCATCGGAACGTTGA
cmx(A) / 2.96 / 10.30 / 12.94 / 7.53 / 6.62 / 10.01 / 11.93 / 5.46 / 7.85 / GCGATCGCCATCCTCTGT
matA/mel / 9.26 / 12.83 / 15.51 / 8.10 / 15.00 / 9.05 / 11.91 / 13.93 / TAGTAGGCAAGCTCGGTGTTGA
msrA / 14.16 / CTGCTAACACAAGTACGATTCCAAAT
oleC / 13.64 / 14.55 / 11.13 / 14.36 / 16.63 / 12.37 / 14.88 / CCCGGAGTCGATGTTCGA
vgaA / 14.60 / 16.60 / CGAGTATTGTGGAAAGCAGCTAGTT
vgaB / 16.09 / TAAAAGAGAATAAGGCGCAAGGA
erm(36) / 10.05 / 11.81 / 13.99 / 9.48 / 17.92 / 8.56 / 13.26 / GGCGGACCGACTTGCAT
ermA / 15.80 / 9.25 / 14.02 / TTGAGAAGGGATTTGCGAAAAG
ermA/ ermTR / 13.82 / 15.64 / ACATTTTACCAAGGAACTTGTGGAA
ermB / 14.24 / TAAAGGGCATTTAACGACGAAACT
ermC / 16.66 / 16.49 / 17.93 / TTTGAAATCGGCTCAGGAAAA
ermK / 13.28 / GTTTGATATTGGCATTGTCAGAGAAA
ermX / 5.13 / 10.29 / 9.31 / 15.10 / 15.07 / 14.70 / 8.22 / 12.95 / CTCGGGTACCAAGGTCTGCTT
ermX / 4.54 / 13.98 / 8.84 / 15.87 / 15.15 / 14.61 / 7.77 / 12.11 / GCTCAGTGGTCCCCATGGT
ermY / 16.26 / 12.37 / TTGTCTTTGAAAGTGAAGCAACAGT
pikR2 / 16.09 / 15.46 / TCGTGGGCCAGGTGAAGA
ereA / 13.03 / 16.25 / 13.95 / 14.90 / 12.80 / 14.59 / CCTGTGGTACGGAGAATTCATGT
vgb / 15.86 / 14.97 / AGGGAGGGTATCCATGCAGAT
erm(34) / 16.50 / GCGCGTTGACGACGATTT
mefA / 6.48 / 11.86 / 13.14 / 4.08 / 11.97 / 13.41 / 2.07 / 8.80 / 13.15 / CCGTAGCATTGGAACAGCTTTT
mphA / 11.25 / 14.67 / 12.23 / 14.67 / 17.66 / 11.62 / 13.44 / CTGACGCGCTCCGTGTT
mphA / 11.38 / 16.16 / 12.55 / 16.08 / 17.77 / 12.09 / 15.09 / TGATGACCCTGCCATCGA
mphB / 14.20 / 14.90 / CGCAGCGCTTGATCTTGTAG
lnuA / 15.04 / TGACGCTCAACACACTCAAAAA
vatC / 15.12 / CGGAAATTGGGAACGATGTT
vatE / 7.65 / 9.94 / 10.68 / 16.77 / 15.32 / GACCGTCCTACCAGGCGTAA
vatE / 16.30 / 8.88 / 10.20 / 16.69 / 14.97 / GGTGCCATTATCGGAGCAAAT
marR / 18.18 / GCGGCGTACTGGTGAAGCTA
sdeB / 15.43 / 15.19 / 14.35 / 12.25 / 11.28 / 11.97 / CACTACCGCTTCCGCACTTAA
mepA / 15.40 / ATCGGTCGCTCTTCGTTCAC
emrD / 16.71 / 14.50 / CTCAGCAGTATGGTGGTAAGCATT
floR / 4.04 / 9.95 / 11.10 / 5.41 / 5.73 / 9.41 / 8.50 / 2.61 / 5.00 / ATTGTCTTCACGGTGTCCGTTA
mdetl1 / 17.71 / ATACAGCAGTGGATATTGGTTTAATTGT
rarD / 10.06 / 13.08 / 12.07 / 11.83 / 15.64 / TGACGCATCGCGTGATCT
yceE/ mdtG / 10.34 / 13.13 / 12.67 / 11.51 / TGGCACAAAATATCTGGCAGTT
yceL/ mdtH / 9.98 / 12.90 / 12.63 / 11.53 / CGCGTGAAACCTTAAGTGCTT
yceL/ mdtH / 9.87 / 13.44 / 13.05 / 11.62 / 15.76 / CTGCCGTTAAATGGATGTATGC
yidY/ mdtL / 9.84 / 12.38 / 11.40 / 9.41 / TGCTGATCGGGATTCTGATTG
ttgB / 14.43 / 16.72 / 12.16 / 11.02 / TCGCCCTGGATGTACACCTT
acrA / 17.50 / CAGACCCGCATCGCATATT
acrA / 9.93 / 12.71 / 11.73 / 12.07 / GGTCTATCACCCTACGCGCTATC
acrA / 10.03 / 13.25 / 12.64 / 12.28 / CAACGATCGGACGGGTTTC
acrA / 16.79 / 13.99 / 13.45 / 15.00 / 16.90 / 13.96 / CGTGCGCGAACGAACA
acrA / 16.37 / 14.53 / 12.94 / 16.78 / 16.94 / 14.15 / TACTTTGCGCGCCATCTTC
acrB / 10.40 / 13.17 / 11.85 / 11.42 / AGTCGGTGTTCGCCGTTAAC
acrF / 9.70 / 12.38 / 11.96 / 10.65 / GCGGCCAGGCACAAAA
acrR / 13.54 / 13.94 / 13.40 / 12.44 / GATGATACCCCCTGCTGTGAGA
adeA / 16.56 / CAGTTCGAGCGCCTATTTCTG
ceoA / 16.43 / 14.90 / ATCAACACGGACCAGGACAAG
mdtE/ yhiU / 9.89 / 12.57 / 12.29 / 11.37 / CGTCGGCGCACTCGTT
mexA / 13.50 / 7.85 / 15.70 / AGGACAACGCTATGCAACGAA
mexE / 14.44 / 13.11 / 14.54 / 8.29 / 14.69 / GGTCAGCACCGACAAGGTCTAC
mexF / 13.14 / 12.47 / 8.15 / 10.67 / 10.17 / 7.79 / 9.39 / 9.19 / 7.84 / CCGCGAGAAGGCCAAGA
mtrD / 17.84 / 15.47 / 17.97 / 17.35 / GGTCGGCACGCTCTTGTC
oprD / 16.74 / 14.85 / 13.74 / 15.63 / 14.08 / 13.07 / 13.37 / 12.08 / ATGAAGTGGAGCGCCATTG
oprJ / 17.97 / 11.33 / 13.70 / 10.96 / 12.59 / ACGAGAGTGGCGTCGACAA
tolC / 17.00 / 16.06 / GCCAGGCAGAGAACCTGATG
tolC / 10.45 / 12.54 / 12.48 / 11.66 / GGCCGAGAACCTGATGCA
tolC / 17.66 / 17.64 / 15.61 / 16.75 / CAGGCAGAGAACCTGATGCA
ttgA / 17.89 / 16.62 / 12.11 / ACGCCAATGCCAAACGATT
qacE∆1 / 15.95 / 14.95 / 4.34 / TCGCAACATCCGCATTAAAA
qacE∆1 / 2.96 / 7.17 / 9.28 / 5.82 / 11.40 / 9.34 / 3.49 / 4.12 / CCCCTTCCGCCGTTGT
qacH / 10.54 / 16.21 / 13.65 / 16.32 / 17.82 / 11.53 / 15.20 / CATCGTGCTTGTGGCAGCTA
qacH / 8.24 / 14.12 / 15.55 / 11.79 / 7.92 / 15.28 / 10.38 / 8.65 / 13.43 / GTGGCAGCTATCGCTTGGAT
catB3 / 7.20 / 13.20 / 15.60 / 8.40 / 8.55 / 14.77 / 14.38 / 8.49 / 10.48 / GCACTCGATGCCTTCCAAAA
catB8 / 9.52 / 14.83 / 15.51 / 10.75 / 10.30 / 14.38 / 17.86 / 10.13 / 11.56 / CACTCGACGCCTTCCAAAG
cfr / 15.08 / 9.98 / 17.68 / 15.83 / GCAAAATTCAGAGCAAGTTACGAA
uidA / 9.93 / 13.23 / 12.78 / 12.08 / AACCACGCGTCTGTTGACTG
ereB / 16.38 / 15.74 / 17.59 / GCTTTATTTCAGGAGGCGGAAT
bacA / 9.91 / 12.59 / 11.72 / 11.71 / TTCCACGACACGATTAAGTCATTG
catA1 / 12.52 / 13.89 / 13.94 / 14.53 / 15.44 / GGGTGAGTTTCACCAGTTTTGATT
pncA / 17.89 / 14.45 / 15.42 / 14.56 / 15.66 / 13.95 / GCAATCGAGGCGGTGTTC
qnrA / 17.14 / 16.99 / AGGATTTCTCACGCCAGGATT
sat4 / 15.94 / GAATGGGCAAAGCATAAAAACTTG
dfrA1 / 6.19 / 11.59 / 12.95 / 8.29 / 9.53 / 13.07 / 14.58 / 4.71 / 8.56 / GGAATGGCCCTGATATTCCA
dfrA12 / 9.41 / 15.80 / 15.59 / 11.84 / 11.97 / 15.23 / 11.45 / 5.67 / 12.04 / CCTCTACCGAACCGTCACACA
folA / 18.22 / 17.58 / CGAGCAGTTCCTGCCAAAG
sul1 / 13.22 / CAGCGCTATGCGCTCAAG
sul2 / 2.64 / 8.23 / 7.70 / 5.88 / 4.27 / 6.88 / 8.52 / 2.60 / 4.05 / TCATCTGCCAAACTCGTCGTTA
tet(35) / 16.31 / ACCCCATGACGTACCTGTAGAGA
tetX / 3.18 / 15.36 / 5.63 / 14.30 / 5.52 / 3.06 / AAATTTGTTACCGACACGGAAGTT
tet(32) / 6.33 / 15.48 / 3.57 / 12.23 / 14.77 / 5.06 / 10.89 / 13.44 / CCATTACTTCGGACAACGGTAGA
tet(36) / 6.88 / 15.50 / 11.65 / 16.30 / 15.72 / AGAATACTCAGCAGAGGTCAGTTCCT
tet(36) / 6.97 / 15.25 / 10.71 / 15.02 / 15.25 / 16.05 / TGCAGGAAAGACCTCCATTACAG
tetM / 3.80 / 4.50 / 9.87 / 8.68 / 14.29 / CATCATAGACACGCCAGGACATAT
tetM / 3.90 / 10.17 / 14.13 / 5.36 / 10.17 / 12.33 / 10.91 / 8.25 / 10.89 / TAATATTGGAGTTTTAGCTCATGTTGATG
tetO / 6.20 / 14.19 / 5.01 / 10.08 / 13.78 / 4.37 / 6.36 / 14.16 / ATGTGGATACTACAACGCATGAGATT
tetPB / 16.58 / ACACCTGGACACGCTGATTTT
tetPB / 11.69 / 14.43 / 13.16 / 14.29 / TGGGCGACAGTAGGCTTAGAA
tetQ / 3.29 / 1.98 / 15.27 / 0.50 / 10.30 / 15.48 / CGCCTCAGAAGTAAGTTCATACACTAAG
tetS / 17.08 / TTAAGGACAAACTTTCTGACGACATC
tetT / 15.73 / CCATATAGAGGTTCCACCAAATCC
tetW / 5.99 / 14.95 / 3.64 / 10.14 / 10.93 / 4.19 / 9.62 / 12.16 / CAACATTAACGGAAAGTTTATTGTATACCA
tetW / 4.55 / 11.96 / 14.32 / 1.60 / 9.60 / 12.53 / 1.65 / 8.93 / 10.95 / ATGAACATTCCCACCGTTATCTTT
tetA / 5.97 / 10.09 / 13.18 / 9.99 / 6.29 / 12.24 / 10.60 / 4.60 / 7.81 / CTCACCAGCCTGACCTCGAT
tetB / 8.92 / 15.10 / 11.65 / 16.53 / 7.53 / 13.14 / GCCCAGTGCTGTTGTTGTCAT
tetB / 9.23 / 15.39 / 11.84 / 16.62 / 6.65 / 14.81 / AGTGCGCTTTGGATGCTGTA
tetC / 15.64 / CATATCGCAATACATGCGAAAAA
tetC / 10.08 / 15.48 / 12.09 / 16.13 / 7.11 / 13.92 / ACTGGTAAGGTAAACGCCATTGTC
tetD / 13.01 / 17.37 / 14.96 / 15.09 / TGTCATCGCGCTGGTGATT
tetD / 13.20 / 17.30 / 15.15 / 17.08 / 14.90 / TGCCGCGTTTGATTACACA
tetE / 15.33 / TTGGCGCTGTATGCAATGAT
tetG / 5.42 / 12.58 / 9.75 / 8.93 / 5.23 / 9.66 / 8.29 / 3.84 / 4.76 / CATCAGCGCCGGTCTTATG
tetG / 5.63 / 14.88 / 15.16 / 8.95 / 5.09 / 13.70 / 9.01 / 2.01 / 7.73 / TCAACCATTGCCGATTCGA
tetH / 6.53 / 15.04 / 8.12 / 15.40 / 16.24 / 10.99 / 15.44 / TTTGGGTCATCTTACCAGCATTAA
tetL / 5.88 / 6.53 / 11.44 / 4.21 / 7.58 / 11.52 / 9.19 / 4.46 / 9.29 / ATGGTTGTAGTTGCGCGCTATAT
tetR / 10.35 / 12.14 / 16.82 / 13.02 / CGCGATGGAGCAAAAGTACAT
tetR / 6.96 / 12.19 / 15.42 / 11.60 / 7.93 / 10.36 / 12.24 / 6.61 / 10.25 / CGCGATAGACGCCTTCGA
tetV / 15.60 / 14.86 / GCGGGAACGACGATGTATATC
tp614, IS4 family / 7.91 / 2.49 / 16.07 / 0.77 / 13.36 / 14.21 / GGAAATCAACGGCATCCAGTT
tnpA, IS6 group / 3.17 / 11.80 / 13.66 / 1.07 / 10.70 / 13.13 / 4.78 / 6.10 / 10.18 / TGCAGATGGTTTAACCTTGGATATTT
tnpA, IS21 group / 10.70 / 15.97 / 14.21 / 12.26 / 14.94 / 14.77 / 11.73 / 10.22 / 10.98 / CATCATCGGACGGACAGAATT
tnpA, IS4 / 4.31 / 11.12 / 15.35 / 8.80 / 8.33 / 14.47 / 9.47 / 5.66 / 10.90 / GGGCGGGTCGATTGAAA
tnpA, ISEcp1B / 4.23 / 14.28 / 8.15 / 15.05 / 14.82 / 12.20 / 9.39 / 12.02 / GAAACCGATGCTACAATATCCAATTT
tnpA, IS6 group / 2.78 / 10.66 / 12.42 / 5.52 / 5.95 / 11.22 / 6.36 / 3.98 / 6.75 / GCCGCACTGTCGATTTTTATC
tnpA, IS6 group / 2.11 / 3.86 / 8.05 / 6.07 / 1.73 / 7.49 / 9.69 / 0.88 / 5.11 / CCGATCACGGAAAGCTCAAG
tnpA, IS6 group / 6.07 / 15.63 / 7.55 / 15.96 / 15.21 / 16.51 / 11.76 / 14.59 / AATTGATGCGGACGGCTTAA
tnpA, IS613 group / 2.32 / 16.08 / 6.67 / 11.67 / 15.09 / 5.13 / 10.44 / 15.16 / AGGTTCGGACTCAATGCAACA
vanRA / 12.62 / 14.06 / CCCTTACTCCCACCGAGTTTT
vanRA / 14.79 / 15.72 / CCACTCCGGCCTTGTCATT
vanB / 15.15 / 17.73 / CCGGTCGAGGAACGAAATC
vanB / 17.84 / 14.42 / 16.75 / 16.79 / TTGTCGGCGAAGTGGATCA
vanHB / 17.62 / 16.06 / 16.28 / GAGGTTTCCGAGGCGACAA
vanRB / 15.95 / 17.51 / GCCCTGTCGGATGACGAA
vanSB / 14.99 / 16.76 / GCGCGGCAAATGACAAC
vanWB / 16.45 / 17.49 / CGGACAAAGATACCCCCTATAAAG
vanXB / 15.44 / 17.59 / AGGCACAAAATCGAAGATGCTT
vanYB / 17.48 / 17.92 / GGCTAAAGCGGAAGCAGAAA
vanC / 17.91 / 15.00 / ACAGGGATTGGCTATGAACCAT
vanC / 17.48 / 17.58 / 15.29 / AAATCAATACTATGCCGGGCTTT
vanC2/ vanC3 / 17.73 / 14.82 / TTTGACTGTCGGTGCTTGTGA
vanSC / 17.88 / 15.02 / GCCATCAGCGAGTCTGATGA
vanTC / 15.08 / CACACGCATTTTTTCCCATCTAG
vanTC / 16.04 / 15.60 / 14.69 / 14.77 / ACAGTTGCCGCTGGTGAAG
vanYD / 17.88 / CAAACGGAAGAGAGGTCACTTACA
vanYD / 17.83 / 15.17 / 16.90 / AAGGCGATACCCTGACTGTCA
vanTE / 18.01 / GTGGTGCCAAGGAAGTTGCT
vanTG / 17.51 / CGTGTAGCCGTTCCGTTCTT
vanWG / 16.21 / 16.03 / ACATTTTCATTTTGGCAGCTTGTAC
1. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3(6):1101–1108.