DOCSLIB.ORG

An Allatotropin-Like Neuropeptide in Mesostoma Ehrenbergii (Rhabdocoela, Platyhelminthes) Mariana Laura Adami, Cristina Damboren

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
An Allatotropin-Like Neuropeptide in Mesostoma Ehrenbergii (Rhabdocoela, Platyhelminthes) Mariana Laura Adami, Cristina Damboren An allatotropin-like neuropeptide in Mesostoma ehrenbergii (Rhabdocoela, Platyhelminthes) Mariana Laura Adami, Cristina Damborenea & Jorge Rafael Ronderos Zoomorphology Evolutionary, Comparative and Functional Morphology ISSN 0720-213X Volume 131 Number 1 Zoomorphology (2012) 131:1-9 DOI 10.1007/s00435-012-0146-3 1 23 Your article is protected by copyright and all rights are held exclusively by Springer- Verlag. This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your work, please use the accepted author’s version for posting to your own website or your institution’s repository. You may further deposit the accepted author’s version on a funder’s repository at a funder’s request, provided it is not made publicly available until 12 months after publication. 1 23 Author's personal copy Zoomorphology (2012) 131:1–9 DOI 10.1007/s00435-012-0146-3 ORIGINAL PAPER An allatotropin-like neuropeptide in Mesostoma ehrenbergii (Rhabdocoela, Platyhelminthes) Mariana Laura Adami · Cristina Damborenea · Jorge Rafael Ronderos Received: 6 May 2011 / Revised: 5 January 2012 / Accepted: 10 January 2012 / Published online: 29 January 2012 © Springer-Verlag 2012 Abstract Allatotropin (AT), a neuropeptide isolated on centrations of 10¡14 and 10¡12 M, suggesting that it is actu- the basis of its ability to stimulate the synthesis of juvenile ally acting as a myoregulator. Detection of AT in several hormones (JHs) in insects, has also been found in other groups of protostomes but apparently not in deuterostomes groups. In addition to this function, AT has proved to be suggests that this peptide could be a synapomorphic feature myotropic. In the present study, we analyze its expression of protostomes. Indeed, the presence of AT in organisms in the free-living turbellarian Mesostoma ehrenbergii (Plat- that do not undergo metamorphosis could suggest that it yhelminthes: Typhloplanida) and its probable functional was Wrst involved in myotropic activities, being the induc- relationship with the muscle tissue. The results show the tion of the synthesis of JHs a secondary function. presence of an AT-like peptide in neurons located in diVer- ent regions of the body of the Xatworm. The analysis of the Keywords Mesostoma ehrenbergii · Neuropeptides · presence of the peptide together with phalloidin labeling Myoregulators · Platyhelminthes · Allatotropin · Phalloidin suggests a functional relationship between the peptide and the muscle tissue. This is particularly evident at the level of the pharynx, where the peptide induces contractions at con- Introduction In free-living platyhelminths, neuropeptides seem to be par- Communicated by T. Bartolomaeus. ticularly important as myoregulators acting on muscles at Electronic supplementary material The online version of this the level of digestive and reproductive organs (Gustafsson article (doi:10.1007/s00435-012-0146-3) contains supplementary et al. 2002). In fact, several neuropeptide families have material, which is available to authorized users. been detected and characterized in Platyhelminthes M. L. Adami · C. Damborenea (Gustafsson et al. 2002; Johnston et al. 1996; Kreshchenko Div. Zool. Inv. Museo de La Plata (FCNyM-UNLP), 2008; McVeigh et al. 2009; Mousley et al. 2004; Wikgren La Plata, Argentina and Reuter 1985). Allatotropin (AT), a neuropeptide originally isolated C. Damborenea CONICET, Buenos Aires, Argentina from the brain of a lepidopteran Manduca sexta Linnaeus, 1763 based on its ability to stimulate the synthesis of juve- J. R. Ronderos (&) nile hormones (JHs) (Kataoka et al. 1989), has also been Centro Regional de Estudios Genómicos (CREG), isolated and characterized in several other insect species Universidad Nacional de La Plata, Parque Tecnológico Florencio Varela, Av. Calchaqui km 23,500 (1888), Florencio Varela, (Abdel-latief et al. 2003; Paemen et al. 1991; Park et al. Buenos Aires, Argentina 2002; Scheng et al. 2007; Truesdell et al. 2000; Veenstra e-mail: [email protected]; and Costes 1999). Furthermore, some AT homologous pep- [email protected] tides were identiWed in other groups such as annelids and J. R. Ronderos mollusks (Jing et al. 2010; Veenstra 2011). AT, originally Cátedra Histol. Embriol. Animal (FCNyM-UNLP), isolated from the nervous system, was also detected in epi- La Plata, Argentina thelial tissues (Riccillo and Ronderos 2010; Santini and 123 Author's personal copy 2 Zoomorphology (2012) 131:1–9 Ronderos 2009a, b; Sterkel et al. 2010). Despite the func- probable interaction of the neuropeptide with them, sam- tion as a JHs regulator, AT has proved to be pleiotropic, ples were co-incubated with a rhodamine-phalloidin solu- acting as a cardioaccelerator (Koladich et al. 2002; Rudwall tion (Sigma–Aldrich) (1/1,000). After every step, the et al. 2000; Sterkel et al. 2010; Veenstra et al. 1994), as a specimens were washed (3 times £ 20 min) with PBS-T regulator of ion transport throughout epithelia (Lee et al. (0.05%). For secondary antibody control, primary antibody 1998), and as a myostimulator at the level of the digestive incubation was omitted and replaced with PBS. To reassay system in several insect species (Duve et al. 1999, 2000; primary antibody speciWcity, polyclonal antiserum was pre- Matthews et al. 2007; Santini and Ronderos 2007; Sterkel viously incubated overnight at 4°C with pure AT synthe- et al. 2010). Furthermore, a new activity, controlling the sized by Biopeptide Company (San Diego, CA, USA) release of digestive enzymes, was recently described for the (kindly provided by Dr Fernando G. Noriega), at a concen- peptide (Lwalaba et al. 2009). tration of 20 nmol/ml of diluted antiserum (1/500). Finally, The existence of a peptide similar to AT was recently worms were mounted in Vectashield mounting medium demonstrated (Adami et al. 2011) in free-living platyhelm- (Vector Laboratories, Burlingame, CA, USA). The result- inths belonging to two diVerent groups (Catenulida and ing material was analyzed with a Laser Scan Microscope Macrostomida), suggesting that this family of peptides is Zeiss LSM 510 Meta. The optical sections were obtained present in ancient groups. In the present study, we further from dorsoventral stacks across all the specimen for pano- analyzed the morphological relationship between nervous ramic views, and for particular regions (eg. pharynx), taken and muscle system, analyzing also the functional activity of in a range from 3 to 5 m in distance between each, corre- AT as a myoregulator in Mesostoma ehrenbergii (Focke, sponding to a number ranging between 10 and 15 optical 1836). sections respectively. Contraction assay Materials and methods As a Wrst approach to analyze the myoregulatory function Animals of the AT-like peptide found in M. ehrenbergii, young/ mature specimen were treated with synthetic AT. The The specimens of the free-living platyhelminth, M. ehren- experimental individuals were moved to Mesostoma sp. bergii (Focke, 1836) (Typhloplanida), were collected at a saline solution (Koopowitz 1996) and left in there for 10 rainwater pond in Pereyra Iraola Park (34°51ЈS; 58°03ЈW), or 15 min until they showed normal behavior (i.e., the a locality situated near to the city of La Plata (Buenos Aires individuals were adapted to saline). Once the experimen- Province, Argentina). The specimens were obtained during tal specimens were ready, the saline solution was replaced summer and autumn 2010 and were maintained in the water with a new one containing diVerent doses of AT. The con- from the original site under a 12:12 h light/dark period until centrations assayed ranged from 10¡16 to 10¡8 M. The they were processed. same individual was used for assays with diVerent doses. Between tests, preparations were washed with the control Immunohistochemistry and phalloidin labeling solution up to basal conditions were restored. The peptide goes into the organisms through the epidermal tissue Specimens were Wxed in formaldehyde–phosphate buVered causing an important delay until the response can be saline (PBS) (4%) at 4°C for 12 h, then washed in PBS- observed. Despite that some individuals showed muscle Tween (0.05%) (PBS-T), permeabilized in Triton X-100 activity in a shorter period, most of them were maintained (1%) (24 h at 4°C), and blocked with 3% bovine serum in the experimental medium for 30 min until any really albumin (BSA) for 2 h. The worms were then incubated visible reaction could be seen in the pharynx. Once the overnight at 4°C with a polyclonal antiserum developed activity started, the number of contractions of the pharynx against AT of the Diptera Aedes aegypti (Linnaeus, 1762) were recorded for a 3 min period and the frequency was (1/500 in 3% BSA diluted in PBS-T). The antibody recog- determined as number of contractions/minute. Between nizes the following sequence of amino acids of the A. the diVerent doses applied, the solutions containing the aegypti AT: APFRNSEMMTARGF. The speciWcity of peptide were replaced by fresh saline solution to restore antibody binding was previously demonstrated by Hernan- the individuals to control conditions. Those individuals dez-Martinez et al. (2005) and Santini and Ronderos (2007, that did not properly respond to saline solutions were dis- 2009b). Animals were then incubated with FITC-labeled carded. Some individuals were recorded with a digital goat anti-rabbit secondary antibody (Santa Cruz Biotech- camera adapted to a stereoscopic microscope to show the nology) (1/1,000 in blocking buVer) for 24 h at 4°C. To way in which both the pharynx and the body musculature visualize the arrangement of the muscle Wbers, and the reacted. 123 Author's personal copy Zoomorphology (2012) 131:1–9 3 Results immunizing peptide clearly diminished the staining conWrming previous analysis (data not shown). The analysis reveals the presence of AT immunoreactive cells The expression of AT-like immunoreactivity and the in M. ehrenbergii. The omission of the primary antibody incu- relationships between the neurons producing the neuropeptide bation abolished the labeling, and the preadsorption with the and the muscle Wbers in M.
Load more

Recommended publications
  • I FLATWORM PREDATION on JUVENILE FRESHWATER
    FLATWORM PREDATION ON JUVENILE FRESHWATER MUSSELS A Thesis Presented to the Graduate College of Southwest Missouri State University In Partial Fulfillment of the Requirements for the Master of Science Degree By Angela Marie Delp July 2002 i FLATWORM PREDATION OF JUVENILE FRESHWATER MUSSELS Biology Department Southwest Missouri State University, July 27, 2002 Master of Science in Biology Angela Marie Delp ABSTRACT Free-living flatworms (Phylum Platyhelminthes, Class Turbellaria) are important predators on small aquatic invertebrates. Macrostomum tuba, a predominantly benthic species, feeds on juvenile freshwater mussels in fish hatcheries and mussel culture facilities. Laboratory experiments were performed to assess the predation rate of M. tuba on newly transformed juveniles of plain pocketbook mussel, Lampsilis cardium. Predation rate at 20 oC in dishes without substrate was 0.26 mussels·worm-1·h-1. Predation rate increased to 0.43 mussels·worm-1·h-1 when a substrate, polyurethane foam, was present. Substrate may have altered behavior of the predator and brought the flatworms in contact with the mussels more often. An alternative prey, the cladoceran Ceriodaphnia reticulata, was eaten at a higher rate than mussels when only one prey type was present, but at a similar rate when both were present. Finally, the effect of flatworm size (0.7- 2.2 mm long) on predation rate on mussels (0.2 mm) was tested. Predation rate increased with predator size. The slope of this relationship decreased with increasing predator size. Predation rate was near zero in 0.7 mm worms. Juvenile mussels grow rapidly and can escape flatworm predation by exceeding the size of these tiny predators.
    [Show full text]
  • Platyhelminthes Rhabdocoela
    Molecular Phylogenetics and Evolution 120 (2018) 259–273 Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev Species diversity in the marine microturbellarian Astrotorhynchus bifidus T sensu lato (Platyhelminthes: Rhabdocoela) from the Northeast Pacific Ocean ⁎ Niels W.L. Van Steenkiste , Elizabeth R. Herbert, Brian S. Leander Beaty Biodiversity Research Centre, Department of Zoology, University of British Columbia, 3529-6270 University Blvd, Vancouver, BC V6T 1Z4, Canada ARTICLE INFO ABSTRACT Keywords: Increasing evidence suggests that many widespread species of meiofauna are in fact regional complexes of Flatworms (pseudo-)cryptic species. This knowledge has challenged the ‘Everything is Everywhere’ hypothesis and also Meiofauna partly explains the meiofauna paradox of widespread nominal species with limited dispersal abilities. Here, we Species delimitation investigated species diversity within the marine microturbellarian Astrotorhynchus bifidus sensu lato in the turbellaria Northeast Pacific Ocean. We used a multiple-evidence approach combining multi-gene (18S, 28S, COI) phylo- Pseudo-cryptic species genetic analyses, several single-gene and multi-gene species delimitation methods, haplotype networks and COI conventional taxonomy to designate Primary Species Hypotheses (PSHs). This included the development of rhabdocoel-specific COI barcode primers, which also have the potential to aid in species identification and delimitation in other rhabdocoels. Secondary Species Hypotheses (SSHs) corresponding to morphospecies and pseudo-cryptic species were then proposed based on the minimum consensus of different PSHs. Our results showed that (a) there are at least five species in the A. bifidus complex in the Northeast Pacific Ocean, four of which can be diagnosed based on stylet morphology, (b) the A.
    [Show full text]
  • Metacommunities and Biodiversity Patterns in Mediterranean Temporary Ponds: the Role of Pond Size, Network Connectivity and Dispersal Mode
    METACOMMUNITIES AND BIODIVERSITY PATTERNS IN MEDITERRANEAN TEMPORARY PONDS: THE ROLE OF POND SIZE, NETWORK CONNECTIVITY AND DISPERSAL MODE Irene Tornero Pinilla Per citar o enllaçar aquest document: Para citar o enlazar este documento: Use this url to cite or link to this publication: http://www.tdx.cat/handle/10803/670096 http://creativecommons.org/licenses/by-nc/4.0/deed.ca Aquesta obra està subjecta a una llicència Creative Commons Reconeixement- NoComercial Esta obra está bajo una licencia Creative Commons Reconocimiento-NoComercial This work is licensed under a Creative Commons Attribution-NonCommercial licence DOCTORAL THESIS Metacommunities and biodiversity patterns in Mediterranean temporary ponds: the role of pond size, network connectivity and dispersal mode Irene Tornero Pinilla 2020 DOCTORAL THESIS Metacommunities and biodiversity patterns in Mediterranean temporary ponds: the role of pond size, network connectivity and dispersal mode IRENE TORNERO PINILLA 2020 DOCTORAL PROGRAMME IN WATER SCIENCE AND TECHNOLOGY SUPERVISED BY DR DANI BOIX MASAFRET DR STÉPHANIE GASCÓN GARCIA Thesis submitted in fulfilment of the requirements to obtain the Degree of Doctor at the University of Girona Dr Dani Boix Masafret and Dr Stéphanie Gascón Garcia, from the University of Girona, DECLARE: That the thesis entitled Metacommunities and biodiversity patterns in Mediterranean temporary ponds: the role of pond size, network connectivity and dispersal mode submitted by Irene Tornero Pinilla to obtain a doctoral degree has been completed under our supervision. In witness thereof, we hereby sign this document. Dr Dani Boix Masafret Dr Stéphanie Gascón Garcia Girona, 22nd November 2019 A mi familia Caminante, son tus huellas el camino y nada más; Caminante, no hay camino, se hace camino al andar.
    [Show full text]
  • R E S E a R C H / M a N a G E M E N T Aquatic and Terrestrial Flatworm (Platyhelminthes, Turbellaria) and Ribbon Worm (Nemertea)
    RESEARCH/MANAGEMENT FINDINGSFINDINGS “Put a piece of raw meat into a small stream or spring and after a few hours you may find it covered with hundreds of black worms... When not attracted into the open by food, they live inconspicuously under stones and on vegetation.” – BUCHSBAUM, et al. 1987 Aquatic and Terrestrial Flatworm (Platyhelminthes, Turbellaria) and Ribbon Worm (Nemertea) Records from Wisconsin Dreux J. Watermolen D WATERMOLEN Bureau of Integrated Science Services INTRODUCTION The phylum Platyhelminthes encompasses three distinct Nemerteans resemble turbellarians and possess many groups of flatworms: the entirely parasitic tapeworms flatworm features1. About 900 (mostly marine) species (Cestoidea) and flukes (Trematoda) and the free-living and comprise this phylum, which is represented in North commensal turbellarians (Turbellaria). Aquatic turbellari- American freshwaters by three species of benthic, preda- ans occur commonly in freshwater habitats, often in tory worms measuring 10-40 mm in length (Kolasa 2001). exceedingly large numbers and rather high densities. Their These ribbon worms occur in both lakes and streams. ecology and systematics, however, have been less studied Although flatworms show up commonly in invertebrate than those of many other common aquatic invertebrates samples, few biologists have studied the Wisconsin fauna. (Kolasa 2001). Terrestrial turbellarians inhabit soil and Published records for turbellarians and ribbon worms in leaf litter and can be found resting under stones, logs, and the state remain limited, with most being recorded under refuse. Like their freshwater relatives, terrestrial species generic rubric such as “flatworms,” “planarians,” or “other suffer from a lack of scientific attention. worms.” Surprisingly few Wisconsin specimens can be Most texts divide turbellarians into microturbellarians found in museum collections and a specialist has yet to (those generally < 1 mm in length) and macroturbellari- examine those that are available.
    [Show full text]
  • When Prey Mating Increases Predation Risk: the Relationship Between the Flatworm Mesostoma Ehrenbergii and the Copepod Boeckella Gracilis
    Arch. Hydrobiol. 163 4 555–569 Stuttgart, August 2005 When prey mating increases predation risk: the relationship between the flatworm Mesostoma ehrenbergii and the copepod Boeckella gracilis Carolina Trochine, Beatriz Modenutti and Esteban Balseiro1 Centro Regional Universitario Bariloche, UN Comahue, Argentina With 4 figures and 4 tables Abstract: The zooplanktivorous flatworm Mesostoma ehrenbergii and the calanoid copepod Boeckella gracilis were observed to coexist in Patagonian fishless ponds. In laboratory experiments, we studied the vulnerability of B. gracilis to M. ehrenbergii predation, testing the attack rates on copulating pairs and single adults in different abundances. We also determined B. gracilis dimorphism, sex ratio and copulating pair ratio on two occasions in a temporary pond, with and without M. ehrenbergii. Our results indicated that B. gracilis exhibited a male-skewed sex ratio irrespective of the presence of the predator. A marked dimorphism characterized this copepod species (females are about 40 % larger than males) and a large proportion of adults were observed participating in copulating pairs that lasted for days. M. ehrenbergii ate sim- ilar quantities of single males and females of B. gracilis but significantly more copu- lating pairs. The use of mucus threads allowed Mesostoma to ingest both members of the pairs instead of only one in most attacks. Larger prey may create more turbulence in the water while swimming, so the hydrodynamic signals produced by pairs should be greater than those produced by single individuals, making them more vulnerable. Besides, the attack rates obtained in the different prey abundances showed that en- counter rate is the factor that determines M.
    [Show full text]
  • Ceh Code List for Recording the Macroinvertebrates in Fresh Water in the British Isles
    01 OCTOBER 2011 CEH CODE LIST FOR RECORDING THE MACROINVERTEBRATES IN FRESH WATER IN THE BRITISH ISLES CYNTHIA DAVIES AND FRANÇOIS EDWARDS CEH Code List For Recording The Macroinvertebrates In Fresh Water In The British Isles October 2011 Report compiled by Cynthia Davies and François Edwards Centre for Ecology & Hydrology Maclean Building Benson Lane Crowmarsh Gifford, Wallingford Oxfordshire, OX10 8BB United Kingdom Purpose The purpose of this Coded List is to provide a standard set of names and identifying codes for freshwater macroinvertebrates in the British Isles. These codes are used in the CEH databases and by the water industry and academic and commercial organisations. It is intended that, by making the list as widely available as possible, the ease of data exchange throughout the aquatic science community can be improved. The list includes full listings of the aquatic invertebrates living in, or closely associated with, freshwaters in the British Isles. The list includes taxa that have historically been found in Britain but which have become extinct in recent times. Also included are names and codes for ‘artificial’ taxa (aggregates of taxa which are difficult to split) and for composite families used in calculation of certain water quality indices such as BMWP and AWIC scores. Current status The list has evolved from the checklist* produced originally by Peter Maitland (then of the Institute of Terrestrial Ecology) (Maitland, 1977) and subsequently revised by Mike Furse (Centre for Ecology & Hydrology), Ian McDonald (Thames Water Authority) and Bob Abel (Department of the Environment). That list was subject to regular revisions with financial support from the Environment Agency.
    [Show full text]
  • Can Large Branchiopods Shape Microcrustacean Communities in Mediterranean Temporary Wetlands?
    CSIRO PUBLISHING Marine and Freshwater Research, 2011, 62, 46–53 www.publish.csiro.au/journals/mfr Can large branchiopods shape microcrustacean communities in Mediterranean temporary wetlands? Aline WaterkeynA,B,D, Patrick GrillasB, Maria Anton-PardoC, Bram VanschoenwinkelA and Luc BrendonckA ALaboratory of Aquatic Ecology and Evolutionary Biology, Katholieke Universiteit Leuven, Charles Deberiotstraat 32, 3000 Leuven, Belgium. BTour du Valat, Research Center for Mediterranean Wetlands, Le Sambuc, 13200 Arles, France. CDepartment of Microbiology and Ecology, University of Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain. DCorresponding author. Email: [email protected] Abstract. It was recently suggested that large branchiopods may play a keystone role in temporary aquatic habitats. Using a microcosm experiment manipulating microcrustacean communities of Mediterranean temporary wetlands (Camargue, Southern France), we tested the following hypotheses: (i) large branchiopods (the notostracan Triops cancriformis and the anostracan Chirocephalus diaphanus) can limit microcrustacean densities through both competition and predation; (ii) notostracans create high suspended-matter concentrations through bioturbation, which can negatively impact microcrustaceans; and (iii) the outcome of these biotic interactions is more detrimental at high salinities. We found a strong predatory impact of T. cancriformis on active microcrustacean populations, but also on dormant populations through the consumption of resting eggs. They also preyed on anostracans and their conspecifics and can indirectly have a negative effect on microcrustaceans through bioturbation, probably by impeding filtering capacities. The presence of C. diaphanus also limited most microcrustacean groups, probably through competition and/or predation. We did not find a significant effect of the tested salinity range (0.5–2.5 g LÀ1) on the biotic interactions.
    [Show full text]
  • Species Diversity of Eukalyptorhynch Flatworms (Platyhelminthes, Rhabdocoela) from the Coastal Margin of British Columbia: Polyc
    MARINE BIOLOGY RESEARCH 2018, VOL. 14, NOS. 9–10, 899–923 https://doi.org/10.1080/17451000.2019.1575514 ORIGINAL ARTICLE Species diversity of eukalyptorhynch flatworms (Platyhelminthes, Rhabdocoela) from the coastal margin of British Columbia: Polycystididae, Koinocystididae and Gnathorhynchidae Niels W. L. Van Steenkiste and Brian S. Leander Beaty Biodiversity Research Centre, Departments of Botany and Zoology, University of British Columbia, Vancouver, BC, Canada ABSTRACT ARTICLE HISTORY Kalyptorhynchs are abundant members of meiofaunal communities worldwide, but knowledge Received 11 June 2018 on their overall species diversity and distribution is poor. Here we report twenty species of Accepted 23 January 2019 eukalyptorhynchs associated with algae and sediments from the coastal margin of British Published online 26 February Columbia. Two species, Paulodora artoisi sp. nov. and Limipolycystis castelinae sp. nov., are 2019 new to science and described based on their unique stylet morphology. New observations SUBJECT EDITOR on two morphotypes of Phonorhynchus helgolandicus and two morphotypes of Danny Eibye-Jacobsen Scanorhynchus forcipatus suggest that the different morphotypes represent different species; accordingly, Phonorhynchus contortus sp. nov., Phonorhynchus velatus sp. nov. and KEYWORDS Scanorhynchus herranzae sp. nov. are described here as separate species. Furthermore, we Flatworms; Pacific Ocean; report on the occurrence and morphology of Polycystis hamata, Polycystis naegelii, Kalyptorhynchia; species Austrorhynchus pacificus,
    [Show full text]
  • Nuevas Aportaciones Al Conocimiento De Los Microturbelarios De La Península Ibérica
    Graellsia, 51: 93-100 (1995) NUEVAS APORTACIONES AL CONOCIMIENTO DE LOS MICROTURBELARIOS DE LA PENÍNSULA IBÉRICA F. Farías (*), J. Gamo (*) y C. Noreña-Janssen (**) RESUMEN En el presente trabajo se citan por vez primera para la fauna ibérica siete especies de Microturbelarios pertenecientes a los Órdenes: Macrostomida (Macrostomum rostra- tum), Proseriata (Bothrioplana semperi) y Rhabdocoela (Castradella gladiata, Opis- tomum inmigrans, Phaenocora minima, Microdalyellia kupelweiseri y M. tenennsensis). Otras cinco especies se citan por segunda vez: Prorhynchus stagnalis (O. Lecithoepitheliata), Opisthocystis goettei, Castrella truncata, Mesostoma ehrenbergii y Rhynchomesostoma rostratum (O. Rhabdocoela). El material estudiado fue recogido en ocho localidades de las provincias de Avila, Cuenca, Guadalajara, Madrid y Segovia, ofreciéndose nuevos datos sobre la autoecología y distribución de estas especies. Palabras clave: Microturbelarios, Faunística, Península Ibérica. ABSTRACT New records of microturbelarians in the Iberian Peninsula. In this study, seven species of freshwater Microturbellaria are recorded for the first time from the Iberian fauna, belonging to the Orders: Macrostomida (Macrostomum ros- tratum), Proseriata (Bothrioplana semperi) and Rhabdocoela (Castradella gladiata, Opistomum inmigrans, Phaenocora minima, Microdalyellia kupelweiseri and M. tenennsensis). Other five species are recorded for the second time: Prorhynchus stagna- lis (O. Lecithoepitheliata), Opisthocystis goettei, Castrella truncata, Mesostoma ehren- bergii
    [Show full text]
  • First Report on Rhabdocoela (Rhabditophora) from the Deeper Parts of the Skagerrak, with the Description of Four New Species
    First report onRhabdocoela (Rhabditophora) from the deeper parts ofthe Skagerrak, with the description offour new species Maria Sandberg Degree project inbiology, 2007 Examensarbete ibiologi, 20p, 2007 Biology Education Centre and Department ofSystematic Zoology, Uppsala University Supervisor: Dr. Wim Willems Table of contents Abstract..........................................................................................................................2 Introduction...................................................................................................................3 Materials and metods ..................................................................................................3 Note ................................................................................................................................4 Abbreviations used in the figures ................................................................................4 Taxonomic results .........................................................................................................4 Kalyptorhynchia ......................................................................................................4 Acrumenidae .....................................................................................................4 Acrumena spec.............................................................................................4 Gnathorhynchidae ............................................................................................5 Prognathorhynchus dubius
    [Show full text]
  • Mesostoma Ehrenbergii Spermatocytes - a Unique and Advantageous Cell for Studying Meiosis AUTHORS: Jessica Ferraro-Gideon1, Carina Hoang 1 and Arthur Forer1
    TITLE: Mesostoma ehrenbergii spermatocytes - a unique and advantageous cell for studying meiosis AUTHORS: Jessica Ferraro-Gideon1, Carina Hoang 1 and Arthur Forer1 AUTHORS AFFILIATIONS: 1Department of Biology, York University, Toronto, ON M3J 1P3, Canada CORRESPONDENCE INFORMATION: Arthur Forer Biology Department, York University 4700 Keele St. Toronto, ON M3J1P3 (905) 736-2100 ext 44643 [email protected] RUNNING TITLE: Meiosis in Mesostoma spermatocytes (44 Characters) KEYWORDS: distance segregation, meiosis, Mesostoma ehrenbergii, oscillations, precocious cleavage furrow, non-random chromosome assortment WORD COUNT (not including references): 2665 1 SUMMARY Mesostoma ehrenbergii have a unique male meiosis: their spermatocytes have three large bivalents that oscillate for 1-2 hours before entering into anaphase without having formed a metaphase plate, have a precocious (“pre-anaphase”) cleavage furrow, and have four univalents that segregate between spindle poles without physical interaction between them, i.e., via “distance segregation”. These unique and unconventional features make Mesostoma spermatocytes an ideal organism for studying the force produced by the spindle to move chromosomes, and to study cleavage furrow control and ‘distance segregation’. In the present article we review the literature on meiosis in Mesostoma spermatocytes and describe the current research that we are doing using Mesostoma spermatocytes, rearing the animals in the laboratory using methods that we describe in our companion article (Hoang et al., 2013). Introduction In the present article, we review the literature on male meiosis in Mesostoma ehrenbergii and describe features of Mesostoma spermatocytes that make them valuable tools for studying cell division. Mesostoma spermatocytes are useful for studying cell division because they have few bivalents and a large spindle.
    [Show full text]
  • Morphology of Udonella Caligorum Johnston, 1835, and the Position of Udonellidae in the Systematics of Platyhelminths
    W&M ScholarWorks Reports 1981 Morphology of Udonella caligorum Johnston, 1835, and the position of Udonellidae in the systematics of platyhelminths A. V. Ivanov Follow this and additional works at: https://scholarworks.wm.edu/reports Part of the Aquaculture and Fisheries Commons, Marine Biology Commons, Oceanography Commons, Parasitology Commons, and the Zoology Commons Recommended Citation Ivanov, A. V. (1981) Morphology of Udonella caligorum Johnston, 1835, and the position of Udonellidae in the systematics of platyhelminths. Translation series (Virginia Institute of Marine Science) ; no. 25. Virginia Institute of Marine Science, William & Mary. https://scholarworks.wm.edu/reports/31 This Report is brought to you for free and open access by W&M ScholarWorks. It has been accepted for inclusion in Reports by an authorized administrator of W&M ScholarWorks. For more information, please contact [email protected]. MORPHOLOGY OF UDONELLA CALIGORUM JOHNSTON, 1835, AND THE (~ , \ POSITION OF UDONELLIDAE IN THE SYSTEMATICS OF PLATYHELMINTHS by A. v. Ivanov Institute of Zoology, Academy of Sciences, USSR Parasitological Collection of the Institute of Zoology Academy of Sciences, USSR XIV, Pages 112-163 1952 Edited by Simmons, J. E., of the University of California at Berkeley and by w. J. Hargis, Jr., and David E. Zwerner of the Virginia Institute of Marine Science Translated by Kassatkin, Maria and Serge Kassatkin University of California at Berkeley Translation Series No. 25 VIRGINIA INSTITUTE OF HARINE SCIENCE College of William and Mary Gloucester Point, Virginia Frank o. Perkins Acting Director 1981 REMARKS ON THE TRANSLATION In 1958 the Parasitology Section of the Virginia Institute of Marine Science undertook to prepare and/or publish (depending on who accomplished the original translation and editing) translations of foreign language papers dealing with important topics.
    [Show full text]