DOCSLIB.ORG

Transcription of SCO-Spondin in the Subcommissural Organ: Evidence for Down-Regulation Mediated by Serotonin

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Transcription of SCO-Spondin in the Subcommissural Organ: Evidence for Down-Regulation Mediated by Serotonin Molecular Brain Research 129 (2004) 151–162 www.elsevier.com/locate/molbrainres Research report Transcription of SCO-spondin in the subcommissural organ: evidence for down-regulation mediated by serotonin Hans G. Richtera,*, Marı´a M. Tome´b, Carlos R. Yulisa, Karin J. Vı´oa, Antonio J. Jime´nezb, Jose´M.Pe´rez-Fı´garesb, Esteban M. Rodrı´gueza aInstituto de Histologı´a y Patologı´a, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile bDepartamento de Biologı´a Celular y Gene´tica, Facultad de Ciencias, Universidad de Ma´laga, Spain Accepted 7 July 2004 Available online 13 August 2004 Abstract The subcommissural organ (SCO) is a brain gland located in the roof of the third ventricle that releases glycoproteins into the cerebrospinal fluid, where they form a structure known as Reissner’s fiber (RF). On the basis of SCO-spondin sequence (the major RF glycoprotein) and experimental findings, the SCO has been implicated in central nervous system development; however, its function(s) after birth remain unclear. There is evidence suggesting that SCO activity in adult animals may be regulated by serotonin (5HT). The use of an anti-5HT serum showed that the bovine SCO is heterogeneously innervated with most part being poorly innervated, whereas the rat SCO is richly innervated throughout. Antibodies against serotonin receptor subtype 2A rendered a strong immunoreaction at the ventricular cell pole of the bovine SCO cells and revealed the expected polypeptides in blots of fresh and organ-cultured bovine SCO. Analyses of organ-cultured bovine SCO treated with 5HT revealed a twofold decrease of both SCO-spondin mRNA level and immunoreactive RF glycoproteins, whereas no effect on release of RF glycoproteins into the culture medium was detected. Rats subjected to pharmacological depletion of 5HT exhibited an SCO-spondin mRNA level twofold higher than untreated rats. These results indicate that 5HT down-regulates SCO-spondin biosynthesis but apparently not its release, and suggest that 5HT may exert the effect on the SCO via the cerebrospinal fluid. D 2004 Elsevier B.V. All rights reserved. Theme: Neurotransmitters, modulators, transporters, and regulators Topic: Serotonin Keywords: Subcommissural organ; SCO-spondin; Serotonin; Serotonin receptors; Gene expression regulation; Bovine; Rat 1. Introduction threadlike structure known as Reissner’s fiber (RF) [17,33,35]. RF extends along the cerebral aqueduct, fourth The subcommissural organ (SCO) is a highly differ- ventricle, and the central canal of the spinal cord. According entiated ependymal gland located in the roof of the third to Rodrı´guez et al. [34], a fraction of the secretory material ventricle, at the entrance of the Sylvian aqueduct. The SCO released at the apical pole of the SCO cells might remain differentiates at early stages of ontogenetic development soluble in the CSF. [24,38] and, with a few exceptions, it remains fully active The primary structure of the major bovine SCO secretory during the entire life span [17,33,35]. The SCO secretes high glycoprotein, SCO-spondin, has been fully established molecular weight glycoproteins [25], which are released into [8,11,18,26]; it is a large N-glycosylated protein (450 the cerebrospinal fluid (CSF) where they condense to form a kDa), encoded by a 14.5-kb mRNA. Several lines of evidence indicate that SCO-spondin plays a role in CNS development [11,12,18,21–23], but the function of the SCO * Corresponding author. Tel.: +56 63 29 3021; fax: +56 63 22 1604. in adulthood is not well understood. Nonetheless, the early E-mail address: [email protected] (H.G. Richter). view that the SCO–RF complex participates in the clearance 0169-328X/$ - see front matter D 2004 Elsevier B.V. All rights reserved. doi:10.1016/j.molbrainres.2004.07.003 152 H.G. Richter et al. / Molecular Brain Research 129 (2004) 151–162 of CSF monoamines [13] has gained strong support from 5HT during the whole culture period (n=8 groups of recent investigations [5,30,36]. explants). The incubation medium together with 5HT was To understand how the SCO secretory activity is changed every 3 days. At the end of the culture period, the regulated may contribute to further elucidate its function(s). explants were subjected to total RNA isolation as described Several neural systems apparently involved in the control of below. the SCO secretory activity have been described [14,27]. The serotonergic input to the SCO originated in raphe nuclei is 2.1.2.2. Effect of 5HT on the expression of RF glycoproteins prominent and has been consistently found in several (SCO-spondin included). Twogroupsof50explants species. According to Le´ger et al. [16] and Mbllgard et al. cultured during 15 days in the absence or presence of 2 [19], serotonin (5HT) participates in the regulation of the AM 5HT were fixed and processed for immunocytochem- SCO secretory activity. istry to demonstrate RF glycoproteins (SCO-spondin The aim of the present investigation was twofold: to included; see below). Two other groups of explants cultured immunodetect 5HT receptors in the SCO and to analyze the during 15 days in the absence or presence of 2 AM 5HT effect of 5HT on SCO-spondin transcription and translation were processed for immunoblotting for the demonstration of under in vivo and in vitro conditions. We found that the RF glycoproteins (see below). bovine SCO expresses 5HT2A receptors that are preferen- tially located at the apical cell pole. Using cultured bovine 2.1.2.3. Effect of 5HT on the release of RF glycoproteins by SCO, it was determined that 5HT down-regulates tran- SCO explants. Fifteen-day-old SCO explants cultured in scription of the SCO-spondin gene and decreases the the absence or presence of 2 AM 5HT were further cultured amount of its translation products, while release of for 1 h in the presence of an antibody against the RF secretory products to the incubation medium was appa- glycoproteins (AFRU, see below), conjugated with fluo- rently not affected. Rats subjected to pharmacological rescein isothiocyanate (40 Al/ml). After washing the living depletion of 5HT exhibited a SCO-spondin mRNA level explants with antiserum-free culture medium, they were about twofold higher than untreated rats. These results observed under a fluorescence microscope to visualize the indicate that 5HT down-regulates SCO-spondin biosyn- immunofluorescent secretion aggregated on the surface of thesis but not release, and suggest that 5HT may exert its the SCO ependymal cells (cf. Ref. [39]). Control explants effect on the SCO via the CSF. The pharmacological and were incubated with FITC-conjugated rabbit IgG. After molecular characterization of the 5HT receptors operating microscopic analysis and photography, all the samples in the SCO needs to be investigated. were fixed in Bouin’s fixative and processed for immuno- cytochemistry as described below, using anti-rabbit IgG as primary antibody. This allowed to visualize, in tissue 2. Material and methods sections, the binding of AFRU to the extracellular secretory material released by the living SCO explants 2.1. In vitro experiments (cf. Ref. [39]). 2.1.1. Tissue sampling 2.1.3. Immunocytochemistry SCOs from 153 adult bovine brains (about 10 min Bovine explants (see above), were fixed by immersion postmortem) were obtained at the local slaughterhouse in Bouin’s fluid for 5 days, and embedded in Paraplast. (FRIVAL, Valdivia). Serial sections, 8-Am thick, were obtained and mounted on gelatin-coated slides. Fresh SCOs, obtained from adult 2.1.2. Organ culture of bovine SCO bovine brains (n=4), were processed in the same way. The immunoperoxidase method of Sternberger et al. [41] was 2.1.2.1. Effect of 5HT on the transcription of SCO-spondin applied using rabbit anti-RF glycoproteins (AFRU, gene. To determine the effect of 5HT (creatinine sulfate A=antibody, FR=fiber of Reissner, U=urea [31])as complex; Sigma, St. Louis, MO, USA) on the expression of primary antibody. The following treatments were sequen- the SCO-spondin gene in the bovine SCO maintained in tially applied to the sections: (1) AFRU (1:10,000) for 18 vitro, about 2400 explants were obtained as described by h; (2) antirabbit IgG developed in goat (E.M. Rodrı´guez, Schoebitz et al. [39]. Twenty-four groups of explants were Instituto de Histologı´a y Patologı´a, Valdivia, Chile), prepared; each experimental group included 100 explants diluted 1:15, 30 min; (3) peroxidase–anti-peroxidase obtained from five SCOs (20 explants/SCO); 15 ml of (PAP; Sigma), diluted 1:75, 30 min; (4) 0.02% 3.3V- serum-free culture medium, prepared according to Schoe- diaminobenzidine tetrahydrochloride (DAB, Sigma) con- bitz et al. [39], were used per culture dish containing 100 taining 0.002% H2O2 for 15 min. All antisera were explants. The following conditions were compared: (1) applied at room temperature, diluted in 0.041 M TRIS freshly prepared noncultured explants (n=8 groups of 100 buffer, pH 7.8, containing 0.7% nongelling seaweed explants each); (2) 15 days of culture, without 5HT (n=8 lambda carrageenan (Sigma) as saturating agent and groups of explants); and (3) 15 days of culture with 2 AM 0.5% Triton X-100. H.G. Richter et al. / Molecular Brain Research 129 (2004) 151–162 153 2.1.4. Western blot of bovine SCO explants using anti-RF 2.2. Serotonin fibers and 5HT2A receptor in the bovine and glycoproteins and anti-5HT2A receptor rat SCO 2.1.4.1. Experimental groups, treatments, and protein The SCO and the region of the brain cortex adjacent to extraction. Seven pools (each pool containing the the ventricle (5-mm distance to the ventricular surface) were explants from four SCOs) were cultured in the presence dissected out from six bovine brains in a local slaughter- (four pools) or absence (three pools) of 2 AM 5HT house (Malaga, Spain; maximum postmortem time period during 15 days, under the conditions described by about 10 min), and immersed in the periodate–lysine– Schoebitz et al.
Load more

Recommended publications
  • Distribution of Gb3 Immunoreactivity in the Mouse Central Nervous System
    Toxins 2010, 2, 1997-2006; doi:10.3390/toxins2081997 OPEN ACCESS toxins ISSN 2072-6651 www.mdpi.com/journal/toxins Article Distribution of Gb3 Immunoreactivity in the Mouse Central Nervous System Fumiko Obata * and Tom Obrig Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 W. Baltimore St. HSFI suite 380, Baltimore, MD 21201, USA; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +1-410-706-6916; Fax: +1-410-706-2129. Received: 25 June 2010 / Accepted: 1 August 2010 / Published: 4 August 2010 Abstract: We have shown previously that neurons in the mouse spinal cord express Gb3. We show in this article that distribution of anti-Gb3-Ab reactivity occurs in many different types of neurons of different areas of the central nervous system (CNS). The immunoreactive neurons are in olfactory bulbs, cerebral cortex, hippocampus, striatum, amygdala, thalamus, hypothalamus, cerebellum, and medulla oblongata. In several different circumventricular organs where vessels do not have the blood-brain-barrier (BBB) structure, anti-Gb3-Ab is not positive for vessel structures, while neurons at these regions are positive. Also, within the ventricular area, ependymal cells in the third ventricle express Gb3, as revealed by anti-Gb3-Ab staining and intensity analysis. Keywords: globotriaosylceramide (Gb3); neuron; circumventricular organs (CVO); ependymal cells 1. Introduction In Shiga-toxin producing Escherichia coli (STEC) infections, a broad spectrum of central nervous system (CNS) symptoms occurs (abbreviations used in this article are listed in Table 1). Those symptoms include cortical blindness, poor fine-motor coordination, seizures and coma [1–13].
    [Show full text]
  • Telencephalic Connections in the Pacific Hagfish (Eptatretus Stouti)
    THE JOURNAL OF COMPARATIVE NEUROLOGY 395:245–260 (1998) Telencephalic Connections in the Pacific Hagfish (Eptatretus stouti), With Special Reference to the Thalamopallial System HELMUT WICHT1* AND R. GLENN NORTHCUTT2 1Klinikum der Johann Wolfgang Goethe-Universita¨t, Dr. Senckenbergische Anatomie, Institut fu¨ r Anatomie II (Experimentelle Neurobiologie), Theodor-Stern-Kai 7, 60590 Frankfurt, Federal Republic of Germany 2Neurobiology Unit, Scripps Institution of Oceanography and Department of Neurosciences, School of Medicine, University of California San Diego, La Jolla, California 9203-0201 ABSTRACT The pallium of hagfishes (myxinoids) is unique: It consists of a superficial ‘‘cortical’’ mantle of gray matter which is subdivided into several layers and fields, but it is not clear whether or how these subdivisions can be compared to those of other craniates, i.e., lampreys and gnathostomes. The pallium of hagfishes receives extensive secondary olfactory projec- tions (Wicht and Northcutt [1993] J. Comp. Neurol. 337:529–542), but there are no experimental data on its nonolfactory connections. We therefore investigated the pallial and dorsal thalamic connections of the Pacific hagfish. Injections of tracers into the pallium labeled many cells bilaterally in the olfactory bulbs. Other pallial afferents arise from the contralateral pallium, the dorsal thalamic nuclei, the preoptic region, and the posterior tubercular nuclei. Descending pallial efferents reach the preoptic region, the dorsal thalamus, and the mesencephalic tectum but not the motor or premotor centers of the brainstem. Injections of tracers into the dorsal thalamus confirmed the presence of reciprocal thalamopal- lial connections. In addition, these injections revealed that there is no ‘‘preferred’’ pallial target for the ascending thalamic fibers; instead, ascending thalamic and secondary olfactory projections overlap throughout the pallium.
    [Show full text]
  • Differential Expression of Five Prosomatostatin Genes in the Central Nervous System of the Catshark Scyliorhinus Canicula
    bioRxiv preprint doi: https://doi.org/10.1101/823187; this version posted October 30, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula Daniel Sobrido-Cameán1, Herve Tostivint2, Sylvie Mazan3, María Celina Rodicio1, Isabel Rodríguez-Moldes1, Eva Candal1, Ramón Anadón1,*, Antón Barreiro-Iglesias1,* 1Department of Functional Biology, CIBUS, Faculty of Biology, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain 2Molecular Physiology and Adaptation. CNRS UMR7221, Muséum National d’Histoire Naturelle, Paris, France 3CNRS, Sorbonne Université, Biologie intégrative des organismes marins (UMR7232- BIOM), Observatoire Océanologique, Banyuls sur Mer, France *Should be considered joint senior authors. Corresponding author: Dr. Antón Barreiro-Iglesias, Department of Functional Biology, CIBUS, Faculty of Biology, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain email: [email protected] Running title: Somatostatin transcripts in the catshark CNS. Acknowledgements: Grant sponsors: Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund 2007-2013 (Grant number: BFU-2017-87079-P to MCR). Agence Nationale de la Recherche (ANR) grant NEMO no ANR-14-CE02-0020-01 (to HT). 1 bioRxiv preprint doi: https://doi.org/10.1101/823187; this version posted October 30, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. ABSTRACT Five prosomatostatin genes (PSST1, PSST2, PSST3, PSST5 and PSST6) have been recently identified in elasmobranchs (Tostivint, Gaillard, Mazan, & Pézeron, 2019).
    [Show full text]
  • Neural Input and Neural Control of the Subcommissural Organ
    MICROSCOPY RESEARCH AND TECHNIQUE 52:520–533 (2001) Neural Input and Neural Control of the Subcommissural Organ 1 2 1 ANTONIO J. JIME´ NEZ, * PEDRO FERNA´ NDEZ-LLEBREZ, AND JOSE MANUEL PE´ REZ-FI´GARES 1Departamento de Biologı´a Celular y Gene´tica, Facultad de Ciencias, Universidad de Ma´laga, Ma´laga, Spain 2Departamento de Biologı´a Animal, Facultad de Ciencias, Universidad de Ma´laga, Ma´laga, Spain KEY WORDS serotonin; GABA; monoamines; pineal organ ABSTRACT The neural control of the subcommissural organ (SCO) has been partially character- ized. The best known input is an important serotonergic innervation in the SCO of several mammals. In the rat, this innervation comes from raphe nuclei and appears to exert an inhibitory effect on the SCO activity. A GABAergic innervation has also been shown in the SCO of the rat and frog Rana perezi. In the rat, GABA and the enzyme glutamate decarboxylase are involved in the SCO innervation. GABA is taken up by some secretory ependymocytes and nerve terminals, coexisting with serotonin in a population of synaptic terminals. Dopamine, noradrenaline, and different neuropeptides such as LH- RH, vasopressin, vasotocin, oxytocin, mesotocin, substance P, ␣-neoendorphin, and galanin are also involved in SCO innervation. In the bovine SCO, an important number of fibers containing tyrosine hydroxylase are present, indicating that in this species dopamine and/or noradrenaline-containing fibers are an important neural input. In Rana perezi, a GABAergic innervation of pineal origin could explain the influence of light on the SCO secretory activity in frogs. A general conclusion is that the SCO cells receive neural inputs from different neurotransmitter systems.
    [Show full text]
  • 54 Cerebellarcerebral Cortex
    Cerebellar and Cerebral Cortex The cerebellar and cerebral hemispheres differ from the spinal cord in that the gray matter is located at the periphery and the white matter lies centrally. Both regions of the brain consist of an outer cortex of gray matter and a subcortical region of white matter. Cerebral Cortex The cerebral cortex is 1.5 to 4.0 mm thick and contains about 50 billion neurons plus nerve processes and supporting glial cells. In all but a few regions it is characterized by a laminated appearance. Perikarya generally are organized into five layers. Starting at the periphery of the cerebral cortex, the general organization of neurons is molecular layer (I), external granular layer (II), external pyramidal layer (III), internal granular layer (IV), internal pyramidal layer (V), and multiform layer (VI). The molecular layer (I) is a largely perikaryon-free zone just below the surface of the cortex. Neurons of similar type tend to occupy the same layer in the cerebral cortex, although each cellular layer is composed of several different cell types. For convenience of description, these neurons often are placed in two major groups: pyramidal cells and stellate or nonpyramidal cells. The perikarya of pyramidal cells are pyramidal in shape and have large apical dendrites that usually are oriented toward the surface of the cerebral cortex and enter the overlying layers; the single axons enter the subcortical white matter. They are found in layers II, III, V, and, to a lesser extent, layer VI. Very large pyramid-shaped neurons (Betz cells) are present in the internal pyramidal layer (V) of the frontal lobe.
    [Show full text]
  • Pial Surface CSF-Contacting Texture, Subpial and Funicular Plexus in the Thoracic Spinal Cord in Monkey: NADPH Diaphorase Histological Configuration
    bioRxiv preprint doi: https://doi.org/10.1101/2020.01.30.927509; this version posted January 31, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. Pial surface CSF-contacting texture, subpial and funicular plexus in the thoracic spinal cord in monkey: NADPH diaphorase histological configuration Yinhua Li1#, Wei Hou1#, Yunge Jia1#, Xiaoxin Wen1, Chenxu Rao1, Ximeng Xu1, Zichun Wei1, Lu Bai1, Huibing Tan1,2* 1 Department of Anatomy, Jinzhou Medical University, Jinzhou, Liaoning 121001, China 2 Department of Neurobiology, Jinzhou Medical University, Jinzhou, Liaoning 121001, China Running title: Funicular neurons in monkey thoracic spinal cord * Correspondence: Department of Anatomy, Jinzhou Medical University, Jinzhou, Liaoning 121001, China, [email protected] # The first three authors make equal contributions to this work. Abstract In spinal cord, white matter is distinguished from grey matter in that it contains ascending and descending axonal tracts. While grey matter gets concentrated with neuronal cell bodies. Notable cell bodies and sensory modality of cerebral spinal fluid (CSF) in white matter are still elusive in certain segment of the spinal cord. Monkey Spinal cord was examined by NADPH diaphorase (NADPH-d) histochemistry. We found that NADPH-d positive neurons clustered and featured flat plane in mediolateral funiculus in caudal thoracic and rostral lumber spinal cord, especially evident in the horizontal sections. Majority of NADPH-d funicular neurons were relatively large size and moderately- or lightly-stained neurons.
    [Show full text]
  • The Value of Early and Comprehensive Diagnoses in a Human Fetus With
    Washington University School of Medicine Digital Commons@Becker Open Access Publications 2016 The value of early and comprehensive diagnoses in a human fetus with hydrocephalus and progressive obliteration of the aqueduct of Sylvius: Case Report Eduardo Ortega Universidad Austral de Chile Rosa I. Munoz Universidad Austral de Chile Nelly Luza Universidad Austral de Chile Francisco Geurra Universidad Austral de Chile Monserrat Guerra Universidad Austral de Chile See next page for additional authors Follow this and additional works at: https://digitalcommons.wustl.edu/open_access_pubs Recommended Citation Ortega, Eduardo; Munoz, Rosa I.; Luza, Nelly; Geurra, Francisco; Guerra, Monserrat; Vio, Karin; Henzi, Roberto; Jaque, Jaime; Rodriguez, Sara; McAllister, James P.; and Rodriguez, Esteban, ,"The aluev of early and comprehensive diagnoses in a human fetus with hydrocephalus and progressive obliteration of the aqueduct of Sylvius: Case Report." BMC Neurology.16,. 45. (2016). https://digitalcommons.wustl.edu/open_access_pubs/4835 This Open Access Publication is brought to you for free and open access by Digital Commons@Becker. It has been accepted for inclusion in Open Access Publications by an authorized administrator of Digital Commons@Becker. For more information, please contact [email protected]. Authors Eduardo Ortega, Rosa I. Munoz, Nelly Luza, Francisco Geurra, Monserrat Guerra, Karin Vio, Roberto Henzi, Jaime Jaque, Sara Rodriguez, James P. McAllister, and Esteban Rodriguez This open access publication is available at Digital Commons@Becker: https://digitalcommons.wustl.edu/open_access_pubs/4835 Ortega et al. BMC Neurology (2016) 16:45 DOI 10.1186/s12883-016-0566-7 CASE REPORT Open Access The value of early and comprehensive diagnoses in a human fetus with hydrocephalus and progressive obliteration of the aqueduct of Sylvius: Case Report Eduardo Ortega1, Rosa I.
    [Show full text]
  • Brain Research, 162 (1979) 219-230 219 ((:~ Elsevier/North-Holland Biomedical Press
    Brain Research, 162 (1979) 219-230 219 ((:~ Elsevier/North-Holland Biomedical Press RETINOFUGAL PATHWAYS IN FETAL AND ADULT SPINY DOGFISH, Squalus acanthias R. GLENN NORTHCUTT Division of Biological Sciences, University of Michigan, Ann Arbor, Mich. 48109 (U.S.A.) (Accepted June 15th, 1978) SUMMARY Retinofugal pathways in fetal and adult spiny dogfish were determined by intra- ocular injection of [3H]proline for autoradiography. Distribution and termination of the primary retinal efferents were identical in pups and adults. The retinal fibers decussate completely, except for a sparse ipsilateral projection to the caudal preoptic area. The decussating optic fibers terminate ventrally in the preoptic area and in two rostral thalamic areas, a lateral neuropil area of the dorsal thalamus and more ventrally in the lateral half of the ventral thalamus. At this same rostral thalamic level, a second optic pathway, the medial optic tract, splits from the lateral marginal optic tract and courses dorsomedially to terminate in the rostral tectum and the central and peri- ventricular pretectal nuclei. The marginal optic tract continues caudally to terminate in a superficial pretectal nucleus and also innervates the superficial zone of the optic tectum. A basal optic tract arises from the ventral edge of the marginal optic tract and courses medially into the central pretectal nucleus, as well as continuing more caudally to terminate in a dorsal neuropil adjacent to nucleus interstitialis and in a more ventrally and medially located basal optic nucleus. Comparison of the retinofugal projections of Squalus with those of other sharks reveals two grades of neural organization with respect to primary visual projections.
    [Show full text]
  • Role of the Subcommissural Organ in the Pathogenesis of Congenital Hydrocephalus in the Htx Rat
    Cell Tissue Res (2013) 352:707–725 DOI 10.1007/s00441-013-1615-9 REGULAR ARTICLE Role of the subcommissural organ in the pathogenesis of congenital hydrocephalus in the HTx rat Alexander R. Ortloff & Karin Vío & Montserrat Guerra & Katherine Jaramillo & Thilo Kaehne & Hazel Jones & James P. McAllister II & Esteban Rodríguez Received: 12 November 2012 /Accepted: 8 March 2013 /Published online: 4 May 2013 # Springer-Verlag Berlin Heidelberg 2013 Abstract The present investigation was designed to clarify nanoLC-ESI-MS/MS. A distinct malformation of the SCO is the role of the subcommissural organ (SCO) in the pathogen- present as early as E15. Since stenosis of the Sylvius aqueduct esis of hydrocephalus occurring in the HTx rat. The brains of (SA) occurs at E18 and dilation of the lateral ventricles starts non-affected and hydrocephalic HTx rats from embryonic day at E19, the malformation of the SCO clearly precedes the 15 (E15) to postnatal day 10 (PN10) were processed for onset of hydrocephalus. In the affected rats, the cephalic and electron microscopy, lectin binding and immunocytochemis- caudal thirds of the SCO showed high secretory activity with try by using a series of antibodies. Cerebrospinal fluid (CSF) all methods used, whereas the middle third showed no signs of samples of non-affected and hydrocephalic HTx rats were secretion. At E18, the middle non-secretory third of the SCO collected at PN1, PN7 and PN30 and analysed by one- and progressively fused with the ventral wall of SA, resulting in two-dimensional electrophoresis, immunoblotting and marked aqueduct stenosis and severe hydrocephalus. The abnormal development of the SCO resulted in the permanent absence of Reissner’s fibre (RF) and led to changes in the A.R.
    [Show full text]
  • Estivill-Torr S Pm
    Int. J. Dev. Biol. 45 (S1): S75-S76 (2001) Short Report PAX6 and MSX1, two homeobox genes involved in the development of the subcommissural organ GUILLERMO ESTIVILL-TORRÚS 1,2, MANUEL FCO. LÓPEZ-ARANDA1, JESÚS M. GRONDONA1, ANTOINE BACH3, BENOîT ROBERT3, TANIA VITALIS2, DAVID J. PRICE2, CASTO RAMOS4, EDUARDO SORIANO4 and PEDRO FERNÁNDEZ-LLEBREZ*1 1 Laboratorio de Fisiología Animal, Departamento de Biología Animal, Facultad de Ciencias, Universidad de Málaga, España, 2Developmental Biology Laboratory, Department of Biomedical Sciences, University of Edinburgh Medical School, Edinburgh, UK, 3Unité Génetique Moléculaire de la Morphogenèse and CNRS URA 1947, Institut Pasteur, Paris, France and 4Departamento de Biología Celular, Facultad de Medicina, Universidad Central de Barcelona ABSTRACT During mouse central nervous system (CNS) (Rodríguez and Yulis, 2001) and the SCO location, have leaded us development, the homeobox -containing genes Pax6 and Msx1, to hypothesize a interrelationship between the SCO and the poste- have a spatial and temporal restricted expression in the CNS and rior commissure formation. The development and establishment of craniofacial skeleton. Both genes are highly expressed in the glial segments and boundaries patterning the CNS, depend on spatial secretory cells that forms the subcommissural organ (SCO), a and temporal restricted expression of regulatory genes encoding circumventricular organ located at the forebrain-midbrain boundary, numerous proliferation and differentiation factors. SCO develop- in the pretectal dorsal midline neuroepithelium beneath the posterior ment depends on the expression of such genes. To present, some commissure. Pax6 (Small eye, Sey/Sey) and Msx1 (-/-) null mutants of them have been reported to be expressed in the pretectal region homozygous fail to develop the SCO and a normal posterior commis- or its neighbourhood, being good candidates to control SCO and/or sure.
    [Show full text]
  • The Nuclear Pattern of the Non-Tectal Portions of the Midbrain and Isthmus in Ungtjlates
    THE NUCLEAR PATTERN OF THE NON-TECTAL PORTIONS OF THE MIDBRAIN AND ISTHMUS IN UNGTJLATES LOIS A. GILLILAN Dr. Louis Merwin Gelston Fellow, Mrdical School, Universzty of Mickigan, Ann Arbor Department of Anatomy, University of Ptttsburgh, Pennsylvania TWENTY-SIX PLATES (TWENTY-SIX FIGURES) INTRODUCTION The material used for this study of the midbrain of the 16 cin pig (Sus scrofa), the adult sheep (Ovis aries) and the old horse (Equus caballus) consists of serially cut, transverse, toluidin blue sections. For the generous grant which made possible this research the writer wishes to express grateful acknowlcdgmeiit to the Horace H. Rackham School of Graduate Studies of the University of Michigan. Very little literature dealing with the ungulate midbrain has come to our attention. Papers by Solnitzsky ( '38 and '39) deal with the dorsal thalamic, subthalamic, and hypothalamic regions of the pig brain; they contain accounts of the pre- tectal regions, substantia nigra and the rostra1 tip of the red nucleus. Rose ('42 b) has found that the pretectal nucleus in the sheep is represented by two groups of cells. Le Gros Clark ( '26) observed that the Edinger-Westphal nucleus in the sheep was little differentiated, and in the pig th'e cells were only slightly different from the small medial raphe cells with which they become continuous anteriorly. The Edinger-Westphal nucleus in the pig is mostly a midline structure with only occasional bilateral clumping. Tsuchida ( '06) found no Edinger-Westphal nucleus in the sheep and in the horse a few scattered cells only. He observed no nucleus medianns anterior and no true nucleus of Perlia; in the horse no nucleus of 289 290 G.
    [Show full text]
  • Circumventricular Organs Dysregulation Syndrome (CODS)
    Circumventricular organs dysregulation syndrome (CODS) ● 第 70 回日本自律神経学会総会 / 理事長講演 司会：髙橋 昭 Circumventricular organs dysregulation syndrome (CODS) Yoshiyuki Kuroiwaa,b Kew words: aquaporin, cerebrospinal fluid, circadian rhythm, guanine nucleotide coupling protein, transient receptor potential Abstract: The biological origin of circumventricular organs (CVOs) evolutionally goes back to the inverte- brates and even further to plants. The CVOs are classified into sensory CVOs (subfornical organ, organum vasculosum of lamina terminalis, and area postrema) and secretory CVOs (neurohypophysis, pineal gland, subcommissural organ, and median eminence). Physiological mechanisms of life-saving homeostasis arising from CVOs consist of at least the following eight axes; neuroendocrine regulation axis, circadian rhythm regulation axis, innate immune regulation axis, nociceptive response regulation axis, body fluid regulation axis, cognitive regulation axis, locomotive driving regulation axis, and inhibitory regulation axis. Summarizing the above, the CVO physiologically contributes to a wide spectrum of autonomic, endocrine, cognitive, sensory gating, and motor regulations, whose impairments potentially result in the complex symptoms being composed of sleep- related, cardiovascular, gastrointestinal, menstrual, emotional, cognitive, sensory, and motor symptoms. I pro- pose the new clinical concept, “circumventricular organs dysregulation syndrome (CODS)” that is known to be seen in human papilloma virus vaccination-associated neuro-immunopathic syndrome
    [Show full text]