“Apple Cider Vinegar- a Therapeutic Drink with Exceptional Neutraceutical Values and Its Recent Developments”

Home , Apple cider vinegar, Apple juice, Mother of vinegar

Revathi Boddu1, Satish Kumar2, Prasanna Kumar3 and Mahesh Lanka4

1 Research scholar, Food Technology and Nutrition, School of Agriculture, Lovely Professional University, Phagwara, Punjab-144411, India 2 Food Technology and Nutrition, School of Agriculture, Lovely Professional University, Phagwara, Punjab- 144411, India. 3 Research scholar, Food Technology and Nutrition, School of Agriculture, Lovely Professional University, Phagwara, Punjab-144411, India 4 Research scholar, Food Technology and Nutrition, School of Agriculture, Lovely Professional University, Phagwara, Punjab-144411, India

*Corresponding author- REVATHI BODDU

Research scholar, Food Technology and Nutrition, School of Agriculture, Lovely Professional University, Phagwara, Punjab-144411, India Phone number: 8074227073 e-mail: [email protected]

ABSTRACT:

Apple juice vinegar is a promptly accessible item that is effectively ready to be consolidated into suppers. A huge group of examination has shown its advantageous properties as a whole item, just as the capacities of the individual segments acidic corrosive and chlorogenic corrosive. ACV may help with controlling blood glucose and lipids, weight reduction, and hypertension and hence might be useful in the administration of type 2 diabetes. ACV all in all might be more viable than acidic corrosive alone, even though there is little exploration straightforwardly contrasting acidic corrosive and ACV. Utilization of the 'mother of vinegar' may likewise increment advantageous impacts contrasted and ACV without this part. The creation strategy for ACV has been appeared to change the parts of ACV, which may thus influence the gainful characteristics. Further examination might be advantageous here to decide the degree of the impact of the creative strategy. The utilization of ACV may without a doubt be advantageous in the administration of type 2 diabetes. Customary utilization of these solid substances in the eating routine may keep the neuronal cells from oxidative pressure which prompts AD. The motivation behind this survey is to feature the medical advantage job, practical property, and restorative employments of Apple Cider Vinegar.

KEYWORDS: ACV(Apple cider vinegar), Fermentation, Production.

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INTRODUCTION:

Vinegar is usually utilized for pickling of products of the soil and in the arrangement of mayonnaise plate of mixed greens dressings, mustard, and other food sauces. Albeit helpful as a food element for flavour and utilitarian properties, the potential wellbeing benefits of vinegar assortments are driving specialists to additionally think about this since quite a while ago utilized food items (Türker 1963; Tan 2005). Normal utilization of bioactive substances is advanced by numerous healthful specialists and the useful food properties of vinegar have been accounted for in an assortment of scientific and lay distributions. With documentation of the wellbeing benefits of vinegar, a simultaneous expansion sought after for organic product vinegar creation has happened (Mazza and Murooka 2009; Ou and Chang 2009). Useful remedial properties of vinegar portrayed incorporate anti-bacterial Activity, blood pressure reduction, antioxidant activity, decrease in the impacts of diabetes, the anticipation of cardiovascular illness, and expanded energy after exercise (Nishidai and others 2000; Ogawa and others 2000a; Kondo and others 2001a; Shimoji and others 2002; Sugiyama and others 2003a .Vinegar is a fluid item delivered from the heavy drinker and resulting in acetous aging of sugar sources. They have been utilized as cures in numerous societies and have been accounted to give advantageous wellbeing impacts when burned-through consistently((Tesfaye et al., 2002). Such advantages are because of different sorts of polyphenols, micronutrients, and other bioactive mixes found in vinegar that add to their pharmacological impacts, among them, antimicrobial, antidiabetic, antioxidative, antiobesity, and antihypertensive impacts. There are numerous kinds of vinegar around the world, including dark vinegar, rice vinegar, balsamic vinegar, and white wine vinegar(Wai Ho, C., Mat Lazim, A., Fazry,2016). Every one of this vinegar is created utilizing diverse crude materials, yeast strains, and aging systems, consequently giving them their novel tastes and flavors. The primary unpredictable compound in vinegar is acidic corrosive, which gives vinegar its solid, acrid fragrance and flavor. Other unpredictable mixes present in vinegar are predominantly alcohols, acids, esters, aldehydes, and ketones(S., Kalsum Hj Hussain Zaki, U., Joe Lim,2016). The variety of vinegar permits broad applications in food.

The most punctual known utilization of vinegar happened over 10,000 years back (Tan, 2005; Johnston and Gaas, 2006). Hippocrates (460 – 377 BC) suggested vinegar for cleaning ulcerations and for the treatment of injuries (Johnston and Gaas, 2006). In the eighth century, Samurai champions of Japan utilized vinegar as a tonic, as they accepted that vinegar tonic invigorated them power and. John Adams, the second leader of the United States (1735 – 1826) took apple juice at his morning meal consistently. Vinegar was additionally utilized therapeutically for the recuperating of wounds (Budak et al., 2014). Sung Tse, who is credited with the improvement of the field of measurable medication in the tenth century in China, utilized sulfur and vinegar as hand washing operators to forestall contamination (Chan et al., 1993; Tan, 2005). U.S. clinical specialists utilized vinegar to treat numerous sicknesses, including poison ivy, croup, stomach hurt, high fever, and edema in the eighteenth century (Tan, 2005). In the eighteenth century, Durande, a French physicist, effectively created frigid acidic corrosive by concentrating vinegar. By the nineteenth century, Schutzenbach, from Germany, built up a technique for assembling vinegar known as the generator cycle, JETIR2101232 Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org 221

© 2021 JETIR January 2021, Volume 8, Issue 1 www.jetir.org (ISSN-2349-5162) which permitted vinegar to be delivered inside 7 days. In the twentieth century, Hromatka, likewise from Germany, built up an upgraded strategy for vinegar making, called lowered acetification, which uses improved air circulation and mixing to create vinegar throughout a more limited timeframe (Tan, 2005). In 1999, the International Vinegar Museum was opened on the fourth of June in Roslyn, South Dakota, United States of America, which is the principal historical center committed to the subject of vinegar, with displays on its production and employment. The Food Safety and Standards Authority of India (2012) states that vinegars are items gotten by the drunkard and acidic corrosive maturation of any reasonable medium, for example, natural product, malt, or molasses, with or without the expansion of caramel and flavors. They will not be sustained with acidic corrosive. The corrosiveness, determined as the acidic corrosive substance, will not be under 3.75% (m/v), the complete solids (m/v) will at the very least 1.5%, and the all-out debris content will not be under 0.18% (Food Safety and Standards Authority of India, 2012).

APPLE CIDER VINEGAR: vinegar is delivered from crude materials containing sugar or starch in 2-stage aging to at first produce ethanol and consequently produce acidic corrosive. vinegar is created from organic product squeezes, for example, grape, apple, plum, coconut and tomato, rice, and potato. (Booth IR, Kroll RG. 1989)Acidic corrosive microscopic organisms (AAB) are available wherever in the climate. They may proliferate in food materials which contain sugar or in the matured items which contain liquor. Various types of AAB have been confined from different sorts of vinegars including white wine, red wine, soul, juice, conventional balsamic, rice, and modern vinegars, which are created by lowered culture with air circulation( Tan SC. Vinegar Fermentation. Louisiana USA: Louisiana State University; 2005.)

TYPES OF VINEGAR:

Vinegar

White Vinegar Rice Vinegar Balsamic Vinegar Apple cider wine vinegar

Figure:1 Types of vinegar

 White Vinegar: This one is most ordinarily found in Indian kitchens. This vinegar is produced using grain-based ethanol or acidic corrosive, which is blended in with water to make it more solvent and appropriate for cooking.( (Pizarro et al., 2008). Because of its solid flavor, white vinegar is generally used to add sharpness in nourishments and is additionally useful in cleaning around the house.

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 Rice Vinegar : This light yellow assortment is better in taste when contrasted with different vinegars. It is produced using rice wine and is generally used to marinate meats or make plunges and dressings. ((Yano et al., 1997).  Balsamic Vinegar : It is made directly from grapes and contains no liquor. It is blackish in shading and the best of the apparent multitude of assortments of vinegar. You can utilize it for a plate of mixed greens dressing or use as a sauce/plunge for finger nourishments. (Gullo M, et al. 2009)  Apple Cider Vinegar: Other than adding mellow acidity to dishes, apple juice vinegar gives different other medical advantages, particularly for skin and hair((Zou et al., 2012). It is produced using apple juice and accordingly, contains an unmistakable fruity tone. It very well may be utilized to cook non- vegan dishes, oats, smoothies  Wine Vinegar: Red wine vinegar is a touch more impactful and acidic than white wine vinegar. Wine vinegars are generally used to get ready European dishes like sautéed food vegetables and Mexican salsa. (Lee et al., 2013).

Apple juice vinegar, or juice vinegar, is a vinegar produced using aged squeezed apple and utilized in a plate of mixed greens dressings, marinades, vinaigrettes, food additives, and chutneys. It is made by pulverizing apples, at that point crushing out the juice. Microorganisms and yeast are added to the fluid to begin the alcoholic aging cycle, which changes the sugars over to liquor. In a subsequent aging advance, the liquor is changed over into vinegar by acidic corrosive shaping microscopic organisms (Acetobacter species). Acidic corrosive and malic corrosive consolidate to give vinegar its acrid taste. Apple juice vinegar has no restorative or dietary benefit(Heikefelt C.,2011). There is no excellent clinical proof that normal utilization of apple juice vinegar assists with keeping up or lose body weight, or is powerful to oversee blood glucose and lipid levels. Apple juice vinegar has different refreshing properties, including antimicrobial and cancer prevention agent impacts. Additionally, proof proposes it might offer medical advantages, for example, supporting weight reduction, decreasing cholesterol, bringing down glucose levels, and improving the side effects of diabetes. Apple juice vinegar (ACV) contains phenolic exacerbates like catechin, caffeic corrosive, gallic corrosive, chlorogenic acids, and p-coumaric corrosive having high cell reinforcement potential. Phenolic mixes are not basic for endurance but rather can ensure against different persistent infections. Different investigates indicated that phenolic exacerbates fix β amyloid and tau protein-related issues related to AD. Consistent utilization of these sound substances in the eating regimen may keep the neuronal cells from oxidative pressure which prompts AD. The reason for this audit is to feature the medical advantage job, useful property, and helpful employments of Apple Cider Vinegar. (Vinegar Institute, FAQ 2016 [cited 2016).

PRODUCTION OF APPLE CIDER VINEGAR:

An apple vinegar drink was created through blended culture maturation of the combination of squeezed apple (absolute sugar substance of 10%) and grain saccharification fluid (all-out sugar substance of 30%). 0.15% of (NH4)2SO4, 0.15% of KH2PO4, and 0.1% of MgSO4 were added into the squeezed apple grain saccharification fluid combination (1:1, g/g), the all-out sugar substance of which had been acclimated to

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13%( Turker I. 1963). Then the disinfected blend was aged by yeast for 108h at 30 ℃. Toward the finish of the liquor maturation, the liquor level of the aged fluid arrived at 7.8%. For the accompanying acidic maturation, 4.2% of stain A was right off the bat vaccinated into the aged fluid, trailed by 2.8% of stain B after 12h. Moreover, the ideal beginning liquor level of the aged natural product grain blend, aging temperature, shaking speed, starting pH worth, and fluid volume were 5%, 28 ℃,120 r/min, 5.5, and 60 mL/300ml, separately(Tan SC. 2005). Under those conditions, the apple vinegar drink was accomplished with all-out corrosive corrosiveness being of 4.75%( DelCampo G et al,2008).

FERMENTATION PROCESS :

Figure: 2- Production of Apple cider vinegar

ACV can be delivered by a two-venture aging cycle, and this cycle is described by the presence of acidic corrosive at a fixation equivalent to or above 4% . Juice vinegars are commonly 5-6% acidic corrosive . The pH of vinegar will rely upon acidic corrosive fixation and is commonly between 2 – 3.5 (Webb AD. Vinegar,2000). Yeasts at first mature the sugars or starch in crude materials to shape ethanol, which is additionally aged by acidic corrosive microorganisms (AAB) to deliver acidic corrosive. This can be refined with juices/crushes from apples, grapes, coconuts, rice, potato, and others. If starch is the underlying crude material, it will initially be hydrolyzed into a sugar. Contingent upon the technique utilized for the subsequent maturation, vinegar can be delivered as fast as inside 24 hours or might be left for quite a long time to years to age (Budak NH,2014).. The result might be separated and purified preceding utilization. This cycle eliminates and crushes AAB, forestalling the arrangement of 'mother of vinegar'. when unpasteurized vinegar is JETIR2101232 Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org 224

© 2021 JETIR January 2021, Volume 8, Issue 1 www.jetir.org (ISSN-2349-5162) permitted to stay in the item, framing an extracellular cellulose layer which can be viewed as a layer on the outside of the fluid, or as an overcast web-like substance, causing the liquid to seem dinky. It isn't extraordinary to ACV. The creation of ACV can happen precipitously utilizing the normally happening yeasts and microscopic organisms on the outside of the natural product, permitting the drink to be effectively made in the home (Aung MT,2015). The item created in the home will probably contrast in the microbiota, acidic corrosive substance, and different particles given that the unconstrained cycle isn't normalized. Separating and sanitization may not be done and the 'mother' might be devoured or used to vaccinate resulting clusters of vinegar.

FERMENTATION OF ACV WITH AAB:

AAB(Acetic acid bacteria) is a gathering of microorganisms in the family Acetobacteriaceae. AAB are obligate aerobes that stain as Gram-negative or Gramvariable, are catalase-positive, and oxidase negative. The non-spore-forming cells are the bar to ellipsoidal-formed (Sengun and Karabiyikli 2011). AAB has an ideal development temperature scope of 25 °C to 30 °C. Even though the ideal pH development range is 5.0 to 6.5, AAB is accounted for as impervious to acidic conditions under pH 5.0 (Holt and others 1994; Trcek and others 2000; Gullo and Giudici 2008). Acetobacter and Gluconobacter are the 2 fundamental AAB genera and the decision of culture directs vinegar creation techniques. The family Acetobacter oxidizes liquor especially over glucose where as the genus Gluconobacter preferentially oxidizes glucose more promptly than ethanol (Swings 1992; Yamada 2000; Gullo and Giudici 2008). Types of AAB separated from various types of vinegars are introduced in Table 2. Underway of vinegar, AAB expects admittance to oxygen. In the more slow surface strategy for vinegar maturation utilized all the more regularly for conventional vinegar, AAB develops at the interface among air and fluid. In the quicker lowered strategy utilized all the more normally for business vinegar, AAB is provided with oxygen through constant air sparging in the acidifying fluid (Fernandez-Perez and others 2010). AAB may deliver different natural acids including acidic, tartaric, lactic, malic, and citrus extracts as the consequence of the oxidation of sugars and alcohols; be that as it may, acidic corrosive is dominating among these acids (Sengun and Karabiyikli 2011).

PHENOLIC COMPOUNDS FOUND IN DIFFERENT TYPES OF VINEGAR :

Polyphenolic mixes of various gatherings are found in vegetables and natural products. Their biochemical deviations incorporate a huge number of blends from phenolic acids to flavonoids compound. Phenolic builds have distinctive well-being helpful impacts, as solid antimicrobial, anticarcinogenic, and cancer prevention agent properties which are now reported.( Scalbert A,et al 2005) The interest is centered around these accumulates due to consistently higher advantageous wellness properties. Leafy foods likewise have wellbeing advancing filaments, phenolic acids, nutrients, minerals, and flavonoids. Phenolic mixes are not a crucial component for endurance but rather can give long haul insurance against various constant diseases.( Crozier A,et al,2006).

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PHARMOCOLOGICAL APPLICATIONS OF ACV:

 Anticancerous agent:

(Abe et al,2001) surveyed the antitumor substance of apple juice vinegar against Meth-A fibrosarcoma utilizing female Balb/c mice. In (Barth et al,2005) utilized a well-established rat model for convincing colon harm related to colon malignant growth by 1,2-dimethylhydrazine and found that overcast squeezed apple diminished the DNA harm, hyperproliferation and go about as disease forestalling specialist. Pelino squeezed apple stifled the multiplication of bosom disease cells and made cell amassing in the cell cycle G2/M stage. Likewise, the squeezed apple additionally hindered 12-o-tetra-decanoylphorbol-13-acetic acid derivation (TPA) actuated tumor beginning of various cell lines.Apple polyphenols contain procyanidins, flavonoids, epicatechin, catechin which essentially stifled colon disease cells. Polyphenols present in apple juice vinegar have cytotoxicity impacts in human urinary bladder malignancy cells (TSGH-8301) related to apoptosis and oxidative stress. In 2008 Clarissa Gerhauser50 has composed an audited article on the Cancer chemoprotective capability of apple segments and squeezed apple. This audit encased in vivo creature information, just as clinical information, indicated a focused on a component of apple items towards malignancy.

 Antidiabetic agent:

Apple juice vinegar (ACV) that is produced using maturation of Apple juice has been utilized as a people medication for diabetes. Diabetes is the metabolic issue portrayed by the hyperglycemic impact. In 2008, investigations of indicated the defensive impact of ACV on lipid profile in ordinary and diabetic rodents model(Shishehbor F et al,2008). Apple determined items adjust the gut microbiota and improved high-fat eating regimen initiated body weight gain, hyperglycemia, hyperinsulinemia, and other metabolic issues in rat models.( Jiang T et al 2016) The metabolic issues brought about by high-fat eating routine were improved by ACV, which demonstrated antihyperlipidemic impact and forestalled the atherogenic effects.( Bouderbala H et al, 2016).Apple juice vinegar (ACV) created an antihyperglycemic impact by the decrease of incendiary reaction, diminished the oxidative pressure-related markers, and standardized the lipid profiles in the entanglement of Diabetes.( Fathy SM et al,2016) In 2016, investigations (Morgan J et al,2016) watched the defensive impact of ACV on type II diabetes the executives. Because of the Meta examination, it has been accounted for that apple item utilization (squeezed apple, sauce) was associated with a lower commonness of obesity.( Nicklas TA et al,2015).In (Fathy SM et al,2016) played out an investigation of shady squeezed apple and apple strip extricate on rodent pancreas. The squeezed apple actuated antihyperglycemic impacts by the decrease of aggravation, change of the oxidative pressure, and diminished the lipid profile, proposing a valuable objective for diabetes mellitus.

 Anti-inflammatory agent:

In (Nazıroğlu M et al,2014) exhibited that ACV modified serum lipid profile, erythrocyte, and liver film oxidative weight in mice. Dietary flavonoids isolated from apples reduced the disturbance related markers

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like interleukin-11, interleukin-2 in the intestinal tissue of mice. Apple polyphenolic aggravates diminished the fiery reaction of the kidney through the reduction of the articulation and action of COX-2 in the rat model. Apple juice vinegar has various flavonoids and exceptionally successful polyphenolic mixes (Yang et al. 2010; Budak et al. 2011; Denis et al. 2013), which clarifies the cancer prevention agent potential against oxidative pressure, free extreme age in tissues and erythrocytes. In 2011 Budak et al. decided the cholesterol bringing down the impact of apple vinegar in rodents benefited from high-fat weight control plans and assessed the serum fatty substance levels, complete cholesterol, lipoproteins (HDL, LDL, VLDL)

 Hepato protective agent:

Concentrate of apple polyphenolic aggravates diminished the toxic impact of Aluminum in the liver of rat species.( Cheng D et al,2014) Polyphenolic intensifies improved the SOD (Superoxide dismutase) and catalase action related to ATP union. In (Kuźniak VK et al,2015) watched the impact of squeezed apple on hepatocarcinogenic action instigated in rodents. These outcomes demonstrated that squeezed apple ensures liver harm. In (DeGomes MCF et al,2015) an investigation on rodents that displayed that squeezed apple had the option to forestall genotoxicity and oxidative pressure convinced by Cadmium.

 ACV role in decreasing cardiotoxicity :

Atherosclerosis is a persistent infection associated with provocative incitement and oxidative pressure which creates cardiovascular illnesses. In (Wu Z et al,2018) proposed an examination and results demonstrated that cardiovascular well-being items delivered likely impacts with apple juice vinegar to improve atherogenesis, enhance irritation, and decreased triacylglycerol in mice serum. Poly phenolic mixes in apple strip extricate decreased arsenic trioxide incited cardiotoxicity in H9c2 cells. The concentrate modified the movement of SOD, catalase, glutathione, GSH (Glutathione reductase) and caspase (Vineetha VP et al, 2014)Apple admission was related to a lower danger of all constant diseases.( Hodgson JM et al,2016).Polyphenolic exacerbates like chlorogenic corrosive found in apple juice vinegar which diminished the oxidation of lipoproteins (particularly LDLs) and forestalled cardiovascular difficulties.( Laranjinha JA et al,1994)

 Antioxidants, Antimicrobial and Antifungal agent :

It was discovered that ACV utilization expanded cancer prevention agents catalysts including SOD and glutathione peroxidase. In (Lu Y, et al,2000) did an investigation that indicated the cell reinforcement and free extremist searching action of apple juice vinegar containing polyphenolic mixes. In (Seydim AC et al,2016) made a trial model on rodents for assessment of the cancer prevention agent capability of apple juice vinegar and grape vinegar. In (Yagnik et al,2018) watched the antimicrobial movement of ACV against different microbes like E. coli, Staphylococcus aureus, and Candida albicans and checked microbial protein articulation. Apple juice vinegar (ACV) additionally has antifungal movement against candida species associated with dental replacement stomatitis( Mota AC et al,2015) In 2011 Hyson composed a complete survey on apples and apple constituents followed by a connective connection between the apple items with

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© 2021 JETIR January 2021, Volume 8, Issue 1 www.jetir.org (ISSN-2349-5162) various ongoing illnesses and their relationship with human health.( Hyson DA. Et al,2011) This audit contained different in vitro examinations and clinical information investigation upheld to apple items.

 ACV role in Cognitive impairments, Immunomodulator effects:

Several studies from the (Shea et al,2005) produced valuable results of apple juice focused on Alzheimer’s disease-linked with aging. In this study, aging showed impaired memory functions and oxidative markers in the mice's brain. When aged mice received the dilute form of apple cider vinegar in drinking water, there was a significant improvement in cognitive functions and increased antioxidant activity.( Tchantchou F et al,2005) Memory impairment, oxidative stress, and reduction of acetylcholine signaling are the major symbols of Alzheimer’s disease. Apple juice also prevents the reduction of acetylcholine depletion connected with aging and oxidative stress and maintains neuronal communications.( Chan A ei al,2006) In ( Safari et al,2017) performed a study to investigate the Immunomodulatory potency of ACV and found ACV more effective, act as an immunomodulator on the systemic and mucosal immune response.

Effect Uses References Anti microbial effect Used for cleaning and treating Dohar 2003 nail fungus, head lice, warts, Chang and Fang 2007 and ear infections and also used to inhibit pathogenic bacteria on fresh fruits and vegetables

Anti oxidant Effect Bioactive substances such as Nishino 2005 polyphenols and vitamins in different types of vinegar defend against oxidative stress due to their signiﬁcant antioxidant activity

Antidiabetic Effect Acetic acid in vinegar may Fushimi and Sato 2005 prevent the complete digestion of complex carbohydrates by either accelerating gastric emptying or by increasing the uptake of glucose by tissues resulting in reduced blood glucose levels AntitumorEffect Vinegar ingestion indicated a Mimura 2004 protective effect with a decreased risk for esophageal cancer by inhibition of growth of typical human leukemia cells with its potent radical scavenging activity

Cholesterol-loweringeffect Polyphenols such as Lloyd-Jones 2010 chlorogenic acid which is present in high levels in apple cider vinegar could inhibit oxidation of LDLs and improve

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health by preventing cardiovascular diseases Antihypertensiveeffect vinegar residues prevent ACE Nishikawa 2001 activity in the blood pressure regulatory system. Melanoidins, which are synthesized in the ﬁnal stage of the Maillard reaction during traditional balsamic vinegar production, exhibit potential health beneﬁts including antihypertensive activity

Table-1: Therapeutic effects of vinegar

CONCLUSION :

The ACV is a promptly accessible item that is effectively ready to be joined into suppers. A huge assemblage of exploration has shown its helpful properties as a whole item, just as the capacities of the individual parts acidic corrosive and chlorogenic corrosive. ACV may help with controlling blood glucose and lipids, weight reduction, and hypertension and thusly might be useful in the administration of type 2 diabetes. ACV overall might be more compelling than acidic corrosive alone, even though there is little examination legitimately looking at acidic corrosive and ACV. Utilization of the 'mother of vinegar' may likewise increment valuable impacts contrasted and ACV coming up short on this segment. The creation technique for ACV has been appeared to adjust the segments of ACV, which may thusly influence the advantageous characteristics. Further examination might be helpful here to decide the degree of the impact of the creative strategy. The utilization of ACV may for sure be valuable in the administration of type 2 diabetes.

REFERENCES :

Abe K, Arai R, Kushibiki T, Sasaki JI, Matsue H. Antitumor-active, Neutral, Mediumsized Glycan from Apple Vinegar. Food Sci Biotechnol. 2001;10:534-8.

Aung MT, Lee P-R, Yu B, Liu S-Q. Cider fermentation with three Williopsis saturnus yeast strains and volatile changes. Annals of Microbiology. 2015; 65(2): 921-8.

Barth SW, Fähndrich C, Bub A, Dietrich H, Watzl B, Will F, et al. Cloudy apple juice decreases DNA damage, hyperproliferation and aberrant crypt foci development in the distal colon of DMH-initiated rats. Carcinogenesis. 2005;26(8):1414-21.

Booth IR, Kroll RG. 1989. The preservation of foods by low pH. In: Gould GW, editor. Mechanisms of action of food preservation procedures. New York: Elsevier Science Publishers. p 119–60

Bouderbala H, Kaddouri H, Kheroua O, Saidi D. Anti- obesogenic effect of apple cider vinegar in rats subjected to a high fat diet. Ann Cardiol Angeiol. 2016;65(3):208-13.

Budak HN, Kumbul DD, Savas CM, Seydim AC, Tas TK, Ciris IM, et al. Effects of apple cider vinegars produced with different techniques on blood lipids in highcholesterol-fed rats. J Agric Food Chem. 2011;59(12):6638-44.

JETIR2101232 Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org 229

Budak NH, Aykin E, Seydim AC, Greene AK, Guzel-Seydim ZB. Functional properties of vinegar. J Food Sci. 2014; 79(5): R757-64.

Budak, N.H., Aykin, E., Seydim, A.C., Greene, A.K., & Seydim, Z.B.G. (2014). Functional properties of vinegar. Journal of Food Science, 79(5), 757-764.

Chan A, Graves V, Shea T. Apple juice concentrate maintains acetylcholine levels following dietary compromise. J Alzheimers Dis. 2006;9(3):287-91.

Chan A, Shea T. Dietary supplementation with apple juice decreases endogenous amyloid-beta levels in murine brain. J Alzheimers Dis. 2009;16(1):176-71.

Chan A, Shea T. Folate deprivation increases presenilin expression, gammasecretase activity and A-beta levels in murine brain: Potentiation by ApoE deficiency and alleviation by dietary S-adenosyl methionine. J Neurochem. 2007;102(3):753-60.

Chan A, Shea T. Supplementation with apple juice attenutates presenilin1overexpression during dietary and genetically-induced oxidative stress. J Alzheimers Dis. 2006;10(4):353-8.

Chan, E., Ahmed, T.M., Wang, M., & Chan, J.C. (1993). History of medicine and nephrology in Asia. American Journal of Nephrology, 14, 295-301.

Cheng D, Zhu C, Wang C, Xu H, Cao J, Jiang W. Hepatoprotective effects of apple polyphenol extract on aluminum- induced liver oxidative stress in the rat. Can J Physiol Pharmacol. 2014;92(2):109-16.

Crozier A, Yokota T, Jaganath IB, Marks SC, Saltmarsh M, Clifford MN. Secondary metabolites in fruits, vegetables, beverages and other plant based dietary components. In Plant Secondary Metabolites: Occurrence, Structure and Role in the Human Diet. 2006;208-302.

DeGomes MCF, Pidone RFA, Lucke G, Boiago GAP, Fujiyama OCT, Ribeiro DA. Apple juice attenuates genotoxicity and oxidative stress induced by cadmium exposure in multiple organs of rats. J Trace Elem Med Biol. 2015;32:7-12.

DelCampo G, Berregi I, Santos JI, Dueñas M, Irastorza A. Development of alcoholic and malolactic fermentations in highly acidic and phenolic apple musts. Bioresour Technol. 2008;99(8):2857-63.

Denis MC, Furtos A, Dudonne´ S, Montoudis A, Garofalo C, Desjardins Y, et al. Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation. PLoS One. 2013;8(1):e53725.

Fathy SM, Drees EA. Protective effects of Egyptian cloudy apple juice and apple peel extract on lipid peroxidation, antioxidant enzymes and inflammatory status in diabetic rat pancreas. BMC Complement Altern Med. 2016;16(1):8.

Fernandez-P ´ erez R, Torres C, Sanz S, Ruiz-Larrea F. 2010. Strain typing of acetic acid bacteria ´ responsible for vinegar production by the submerged elaboration method. Food Microbiol 27:973–8.

Food Regulations. (1985). Standards and Particular Labelling Requirements for Food: Vinegar Sauce, Chutney and Pickle. Malaysia: Food Act 1983, Food Regulation 1985.

Food Safety and Standards Authority of India. (2012). Manual of Methods of Analysis of Foods. India: Ministry of Health and Family Welfare Government of India.

Food Standards Code; Standard 2.10.1. In: Zealand FSAN, editor. 1991.

Gullo M, Giudici P. 2008. Acetic acid bacteria in traditional Balsamic vinegar: phenotypic traits relevant for starter cultures selection. Intl J Food Microbiol 125:46–53.

Heikefelt C. Chemical and sensory analyses of juice, cider and vinegar produced from different apple cultivars. 2011

JETIR2101232 Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org 230

Hodgson JM, Prince RL, Woodman RJ, Bondonno CP, Ivey KL, Bondonno N, et al. Apple intake is inversely associated with all-cause and disease-specific mortality in elderly women. Br J Nutr. 2016;115(5):860-7.

Holt JM, Krieg NR, Sneath PHA, Staley JY, Williams ST. 1994. Genus Acetobacter and Gluconobacter. In: Wilkens W, editor. Bergey’s manual of determinative bacteriology. 9th ed. Maryland, USA: The Williams & Wilkins Co. p 71–84.

Hyson DA. A Comprehensive Review of Apples and Apple Components and Their Relationship to Human Health. Adv Nutr. 2011;2(5):408-20.

Jiang T, Gao X, Wu C, Tian F, Lei Q, Bi J, et al. Apple- derived pectin modulates gut microbiota, improves gut barrier function and attenuates metabolic endotoxemia in rats with diet-induced obesity. Nutrients. 2016;8(3):126.

Johnston, C.S., & Gaas, C.A. (2006). Vinegar: medicinal uses and antiglycemic effect. Medscape General Medicine, 8(2), 61.

Kondo S, Kuwahara Y, Kondo M, Naruse K, Mitani H, Wakamatsu Y, Ozato K, Asakawa S, Shimizu N, Shima A. 2001a. The medaka rs-3 locus required for scale development encodes ectodysplasin-A receptor. Current Biol 11:1202– 6

Kuźniak VK, Szaefer H, Ignatowicz E, Adamska T, Markowski J, BaerDubowska W. Influence of cloudy apple juice on n-nitrosodiethylamineinduced liver injury and phases I and II biotransformation enzymes in rat liver. Acta Pol Pharm. 2015;72:267-76

Laranjinha JA, Almeida LM, Madeira VM. Reactivity of dietary phenolic acids with peroxyl radicals: Antioxidant activity upon low density lipoprotein peroxidation. Biochem Pharmacol. 1994;48(3):487-94.

Lee, J.Y., Cho, H.D., Jeong, J.H., Lee, M.K., Jeong, Y.K., Shim, K.H., & Seo, K.I. (2013). New vinegar produced by tomato suppresses adipocyte differentiation and fat accumulation in 3T3-L1cells and obese rat model. Food Chemistry, 141, 3241-3249.

Lu Y, Foo LY. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chemistry. 2000;68(1):81-5.

Mazza S, Murooka Y. 2009. Vinegar through the age. In: Solieri L, Giudici P, editors. Vinegars of the world. Milan: Springer-Verlag. p 17 ´ −39.

Morgan J, Mosawy S. The Potential of Apple Cider Vinegar in the Management of Type 2 Diabetes. International Journal of Diabetes Research. 2016;5(6):129-34.

Mota AC, DeCastro RD, DeAraújo OJ, DeOliveira LE. Antifungal Activity of Apple Cider Vinegar on Candida Species Involved in Denture Stomatitis. J Prosthodont. 2015;24(4):296-302.

Nazıroğlu M, Güler M, Özgül C, Saydam G, Küçükayaz M, Sözbir E. Apple cider vinegar modulates serum lipid profile, erythrocyte, kidney and liver membrane oxidative stress in ovariectomized mice fed high cholesterol. J Membr Biol. 2014;247(8):667-73.

Nicklas TA, O’Neil CE, Fulgoni VL. Consumption of various forms of apples is associated with a better nutrient intake and improved nutrient adequacy in diets of children: National Health and Nutrition Examination Survey 2003– 2010. Food Nutr Res. 2015;14:48.

Nishidai S, Nakamura Y, Torikai K. 2000. Kurosu, a traditional vinegar produced from unpolished rice, suppresses lipid peroxidation in vitro and in mouse skin. Biosci Biotechnol Biochem 64:1909–14

Ogawa M, Kusano T, Katsumi M, Sano H. 2000a. Rice gibberellin-insensitive gene homolog, OsGAI, encodes a nuclear-localized protein capable of gene activation at transcriptional level. Gene 245:21–9

Ou ASM, Chang RC. 2009. Taiwan fruit vinegar. In: Solieri L., Giudicin P, editors. Vinegars of the world. Milan: Springer-Verlag. p 223 ´ −41. JETIR2101232 Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org 231

Pizarro, C., Esteban-Díez, I., Sáenz-González, C., & González-Sáiz, J.M. (2008). Vinegar classification based on feature extraction and selection from headspace solid-phase microextraction/gas chromatography volatile analyses: A feasibility study. Analytica Chimica Acta, 608, 38-47.

Rogers EJ, Mihalik S, Ortiz D, Shea T. Apple juice prevents oxidative stress and impaired cognitive performance caused by genetic and dietary deficiencies in mice. J Nutr Health Aging. 2004;8(2):92-7.

S., Kalsum Hj Hussain Zaki, U., Joe Lim, S., Varieties, production, composition and health benefits of vinegars: A review, Food Chemistry (2016)

Safari R, Hoseinifar SH, Nejadmoghadam S, Khalili M. Apple cider vinegar boosted immunomodulatory and health promoting effects of Lactobacillus casei in common carp (Cyprinus carpio). Fish Shellfish Immunol. 2017;67:441-8.

Scalbert A, Manach C, Morand C, Re´me´sy C, Jime´nez L. Dietary polyphenols and the prevention of diseases. Crit Rev Food Sci Nutr. 2005;45(4):287-306.

Sengun IY, Karabiyikli S. 2011. Importance of acetic acid bacteria in food industry. Food ¨ Control 22:647–56.

Seydim AC, Guzel-Seydim ZB, Doguc DK, Savas MC, Budak HN. Effects of grape wine and apple cider vinegar on oxidative and antioxidative status in high cholesterol-fed rats. Functional Foods in Health and Disease. 2016;6(9):569- 77.

Shimoji Y, Oishi E, Kitajima T, Muneta Y, Shimizu S, Mori Y. 2002. Heterologous protein expression and intranasal immunization of pigs. Infect Immun 70:226–32.

Shishehbor F, Mansoori A, Sarkaki AR, Jalali MT, Latifi SM. Apple cider vinegar attenuates lipid profile in normal and diabetic rats. Pak J Biol Sci. 2008;11(23):2634-8.

Sugiyama A, Saitoh M, Takahara A, Satoh Y, Hashimoto K. 2003a. Acute cardiovascular effects of a new beverage made of wine vinegar and grape juice, assessed using an in vivo rat. Nutr Res 23:1291–6.

Swings J. 1992. The genera Acetobacter and Gluconobacter. In: Balows Truper, Dworkin Harder, ¨ Schleifer, editors. The prokaryotes. New York: Springer-Verlag. p 2268–86.

Tan SC. 2005. Vinegar fermentation [Master of Science thesis]. Louisiana State Univ., Dept. of Food Science, Baton Rouge. p 101s.

Tan SC. Vinegar Fermentation. Louisiana USA: Louisiana State University; 2005.

Tchantchou F, Chan A, Kifle L, Ortiz D, Shea T. Apple juice concentrate prevents oxidative damage and impaired maze performance in aged mice. J Alzheimers Dis. 2005;8:283–7.

Tchantchou F. Dietary supplementation with apple juice concentrate alleviates the compensatory increase in glutathione synthase transcription and activity that accompanies dietary- and genetically-induced oxidative stress. J Nutr Health Aging. 2004;8(6):492-6.

Tesfaye, W., Morales, M.L., Garcıá -Parrilla, M.C., & Troncoso, A.M. (2002). Wine vinegar: technology, authenticity and quality evaluation. Trends in Food Science and Technology, 13, 12-21.

The Vinegar Institute, FAQ 2016 [cited 2016]. Available from: http://versatilevinegar.org/faqs/.

Turker I. 1963. Sirke Teknolojisi ve Teknikte Laktik Asit Fermantasyonları. In: Turker I, editor. ¨ Ankara, Turkey: Ankara Univ., Schoolbook of Faculty of Agriculture, Ankara Univ. Press. p 181.

Types- Gullo M, De Vero L, Giudici P. 2009. Succession of selected strains of Acetobacter pasteurianus and other acetic acid bacteria in traditional balsamic vinegar. Appl Environ Microb 75:2585–89.

JETIR2101232 Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org 232

Vineetha VP, Girija S, Soumya RS, Raghu KG. Polyphenol- rich apple (Malus domestica L.) peel extract attenuates arsenic trioxide induced cardiotoxicity in H9c2 cells via its antioxidant activity. Food Funct. 2014;5(3):502-11.

Wai Ho, C., Mat Lazim, A., Fazry Varieties, production, composition and health benefits of vinegars: A review, Food Chemistry (2016)

Webb AD. Vinegar. Kirk-Othmer Encyclopedia of Chemical Technology: John Wiley & Sons, Inc.; 2000.

Wu Z, Xia Z, Wu B, Wei F, Lv X, Xie Y, et al. Lipid profiling in serum from apolipoprotein E-knock out mice fed with different diets and its application to the study of the regulatory effect on lipid metabolism. Food Funct. 2018;9(10):5103- 14.

Yagnik D, Serafin V, Shah AJ. Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans: Downregulating cytokine and microbial protein expression. Scientific Reports. 2018;8(1):1732

Yamada Y. 2000. Transfer of Acetobacter oboediens and Acetobacter intermedius to the genus Gluconacetobacter as Gluconacetobacter oboediens comb. nov. and Gluconacetobacter intermedius comb. nov. Intl J Syst Evol Microbiol 50:2225–7.

Yang J, Li Y, Wang F, Wu C. Hepatoprotective effects of apple polyphenols on CCl4- induced acute liver damage in mice. J Agric Food Chem. 2010;58(10):6525-31.

Yano, T., Aimi, T., Nakano, Y., & Tamai, M. (1997). Prediction of the concentrations of ethanol and acetic acids in the culture broth of a rice vinegar fermentation using nearinfrared spectroscopy. Journal of Fermentation and Bioengineering, 84(5), 461-465.

Zou, X.B., Li, Y.X., Shi, J.Y., Huang, X.W., & Zhao, J.W. (2012). Traditional vinegars identification by colorimetric sensor. Procedia Chemistry, 6, 20-26.

JETIR2101232 Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org 233

Figure 1: www.healthline.com

Figure 2: Alcoholic fermentation by yeast under anaerobic condition. The gene names encoding the various enzymes. Glucose catabolism: 1: hexokinase; 2: glucokinase. Galactose catabolism: 3: galactokinase; 4: galactose-1-phosphate uridylytransferase; 5: UDP-glucose 4- epimerase; 6: phosphoglucomutase. Mannose catabolism: 1, hexokinase I; 7: mannose-6- phophate isomerase. G-3-P, Glyceraldehyde-3-phosphate; DHAP, dihydroxy-acetonephosphate; PEP, phospho-enol pyruvate; PPP, Pentose phosphate pathway. (Source: Maris et al. 2006)

Table 1:

Dohar JE. 2003. Evolution of management approaches for otitis externa. Pediatr Infect Dis J 22:299–308.

Chang J, Fang TJ. 2007. Survival of Escherichia coli O157:H7 and Salmonella enterica serovars typhimurium in iceberg lettuce and the antimicrobial effect of rice vinegar against E. coli O157:H7. Food Microbiol 24:745–51.

Nishino H, Murakoshi M, Mou XY, Wada S, Masuda M, Ohsaka Y, Satomi Y, Jinno K. 2005. Cancer prevention by phytochemicals. Oncology 69:38–40.

Fushimi T, Sato Y. 2005. Effect of acetic acid feeding on the circadian changes in glycogen and metabolites of glucose and lipid in liver and skeletal muscle of rats. Br J Nutr 94:714–9.

Mimura A, Suzuki Y, Toshima Y, Yazaki S, Ohtsuki T, Ui S, Hyodoh F. 2004. Induction of apoptosis in human leukemia cells by naturally fermented sugar cane vinegar (kibizu) of Amami Ohshima Island. Biofactors 22:93–7.

Lloyd-Jones D, Adams RJ, Brown TM, Carnethon M, Dai S, Simone GD, Ferguson TB, Ford E, Furie K, Gillespie C, Go A, Greenlund K, Haase N, Hailpern S, Ho PM, Howard V, Kissela B, Kittner S, Lackland D, Lisabeth L, Marelli A, McDermott MM, Meigs J, Mozaffarian D, Mussolino M, Nichol G, Roger VL, Rosamond W, Sacco R, Sorlie P, Stafford R, Thom T, Wassertheil-Smoller S, Wong ND, Wylie-Rosett J. 2010. Heart disease and stroke statistics—2010 update. Circulation AHA J 121(7):e46.

Nishikawa Y, Takana Y, Nagai Y, Mori T, Kawada T, Ishihara N. 2001. Antihypertensive effects of Kurosu extract, a traditional vinegar produced from unpolished rice, in the SHR rats. Nippon Shokuhin Kagaku Kogaku Kaishi 48:73–5

JETIR2101232 Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org 234