An Overview on Types, Medicinal Uses and Production of Vinegar
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Salad Dressing Ratios
Salad Dressing Ratios L E T ' S E X P L O R E H O W T O M A K E S A L A D D R E S S I N G ! Recipes are a wonderful way to teach yourself how to cook, but sometimes you might be missing an ingredient or you might want to try making up your own recipe. The best way to improvise and create your own recipe is by understanding how different factors can change how food works together. For example fat, salt, acid and heat can all drastically change how our food combines and tastes. Fat Salt Acid Heat Remember the TWK@Home activity (Becoming a Sensory Sleuth) where we learned about “sweet”, “salty”, “sour”, and “bitter” and how each of those elements of taste work together in all sorts of things we eat. Chocolate pretzels are a classic example of salty and sweet, and the combination is what makes them taste so good! The best recipes combine the perfect amount of these flavors to create the food we love. Sweet Salty Sour Bitter When making your own salad dressing you want to find a similar balance. But how do you know how much of each ingredient to use without a recipe? Find more TWK@Home activities at tastewisekids.org Written by Kiah Gibian That's where understanding ratios comes in! A ratio is a way to explain how much of one thing there is compared to another thing. For example a good dressing ratio is: 3 parts Oil and 1 part Vinegar or 3 to 1 or Oil 3:1 Vinegar "Parts can be cups, tablespoons or even liters. -
Acetobacter Sacchari Sp. Nov., for a Plant Growth-Promoting Acetic Acid Bacterium Isolated in Vietnam
Annals of Microbiology (2019) 69:1155–11631163 https://doi.org/10.1007/s13213-019-01497-0 ORIGINAL ARTICLE Acetobacter sacchari sp. nov., for a plant growth-promoting acetic acid bacterium isolated in Vietnam Huong Thi Lan Vu1,2 & Pattaraporn Yukphan3 & Van Thi Thu Bui1 & Piyanat Charoenyingcharoen3 & Sukunphat Malimas4 & Linh Khanh Nguyen1 & Yuki Muramatsu5 & Naoto Tanaka6 & Somboon Tanasupawat7 & Binh Thanh Le2 & Yasuyoshi Nakagawa5 & Yuzo Yamada3,8,9 Received: 21 January 2019 /Accepted: 7 July 2019 /Published online: 18 July 2019 # Università degli studi di Milano 2019 Abstract Purpose Two bacterial strains, designated as isolates VTH-Ai14T and VTH-Ai15, that have plant growth-promoting ability were isolated during the study on acetic acid bacteria diversity in Vietnam. The phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates were located closely to Acetobacter nitrogenifigens RG1T but formed an independent cluster. Methods The phylogenetic analysis based on 16S rRNA gene and three housekeeping genes’ (dnaK, groEL, and rpoB) sequences were analyzed. The genomic DNA of the two isolates, VTH-Ai14T and VTH-Ai15, Acetobacter nitrogenifigens RG1T, the closest phylogenetic species, and Acetobacter aceti NBRC 14818T were hybridized and calculated the %similarity. Then, phenotypic and chemotaxonomic characteristics were determined for species’ description using the conventional method. Results The 16S rRNA gene and concatenated of the three housekeeping genes phylogenetic analysis suggests that the two isolates were constituted in a species separated from Acetobacter nitrogenifigens, Acetobacter aceti,andAcetobacter sicerae. The two isolates VTH-Ai14T and VTH-Ai15 showed 99.65% and 98.65% similarity of 16S rRNA gene when compared with Acetobacter nitrogenifigens and Acetobacter aceti and they were so different from Acetobacter nitrogenifigens RG1T with 56.99 ± 3.6 and 68.15 ± 1.8% in DNA-DNA hybridization, when isolates VTH-Ai14T and VTH-Ai15 were respectively labeled. -
And Acetobacter Pornorurn Spm Nov., Two New Species Isolated from Industrial Vinegar Fermentations
International Journal of Systematic Bacteriology (1 998),48, 93 5-940 Printed in Great Britain Description of Acetobacter oboediens Spm nov, and Acetobacter pornorurn Spm nov., two new species isolated from industrial vinegar fermentations Stephan J. Sokollek, Christian HerteI and Walter P. Hammes Author for correspondence: Walter P. Hammes. Tel: +49 71 1 459 2305. Fax: +49 71 1 459 4255. e-mail: [email protected] lnstitut fur Two strains of Acetobacter sp., LTH 2460Tand LTH 2458T, have been isolated Lebensmitteltechnologie, from running red wine and cider vinegar fermentations, respectively. Universitat Hohenheim, GarbenstraBe 28, D-70599 Taxonomic characteristics of the isolates were investigated. Comparative Stuttg a rt, Germany analysis of the 165 rRNA sequences revealed > 99% similarity between strain LTH 2460Tand the type strains of the related species Acetobacter europaeus and Acetobacter xylinus and between strain LTH 2458Tand Acetobacter pasteurianus. On the other hand, low levels of DNA relatedness (< 34%) were determined in DNA-DNA similarity studies. This relatedness below the species level was consistent with specific physiological characteristics permitting clear identification of these strains within established species of acetic acid bacteria. Based on these results, the names Acetobacter oboediens sp. nov. and Acetobacterpomorum sp. nov. are proposed for strains LTH 2460Tand LTH 2458T, respectively. The phylogenetic positions of the new species are reflected by a 165 rRNA-based tree. Furthermore, a 165 rRNA-targeted oligonucleotide probe specific for A. oboediens was constructed. Keywords: Acetobacter oboediens sp. nov., Acetobacter pomorum sp. nov., vinegar fermentation, acetic acid bacteria INTRODUCTION isolated from vinegar fermentations are not suitable for preservation (Sievers & Teuber, 1995). -
Screening of Acetic Acid Producing Microorganisms from Decomposed Fruits for Vinegar Production
Advances in Microbiology, 2015, 5, 291-297 Published Online May 2015 in SciRes. http://www.scirp.org/journal/aim http://dx.doi.org/10.4236/aim.2015.55028 Screening of Acetic Acid Producing Microorganisms from Decomposed Fruits for Vinegar Production Farzana Diba1, Fahmida Alam1,2, Ali Azam Talukder1* 1Department of Microbiology, Jahangirnagar University, Dhaka, Bangladesh 2Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Malaysia Email: *[email protected], [email protected] Received 11 February 2015; accepted 5 May 2015; published 7 May 2015 Copyright © 2015 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/ Abstract Acetic acid bacteria capable of growing at 30˚C - 37˚C were collected from various decomposed fruits available in Bangladeshi local markets in order to assess their suitability for vinegar pro- duction. Initially, 42 microorganisms were isolated from decomposed fruits like grapes, mangoes, pineapples, oranges, safeda etc. during summer when temperature reaches up to 37˚C. Then their growths were checked in YPG medium containing various ethanol concentrations at different time point at 37˚C. From the preliminary screening, 15 Gram negative bacterial isolates have produced halos or yellow zone around the colonies on YPG agar plate at 37˚C which indicated acetic acid production capability by those bacteria. Furthermore, acetic acid production rates were deter- mined by titration method and about 3 - 6.9 gm/100ml acetic acid were estimated by using 4% ethanol at 37˚C by shaking culture for 3 days. -
Isolation and Characterization of Acetobacter Aceti from Rotten Papaya
J. Bangladesh Agril. Univ. 13(2): 299–306, 2015 ISSN 1810-3030 Isolation and characterization of Acetobacter aceti from rotten papaya J. Kowser*, M. G. Aziz and M. B. Uddin Department of Food Technology and Rural Industries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, *Email: [email protected] Abstract The present study was concerned with the isolation and characterization of Acetobacter aceti from rotten papaya for vinegar production. The samples were inoculated in sterilized GYC standard media and then incubated at 30°C for 48 hours. Successive subculture was performed to screen out the strains. In Gram’s staining, the morphology of the isolated bacteria exhibited pink, small rod shaped single, pair and chain in arrangement, in the hanging drops technique, all the isolates revealed motile. Biochemical tests were performed by fermentation of five basic sugars by producing both acid and gas bubbles in Durham tube. All of the isolates were Indole, Voges-Proskauer (VP) and Oxidase negative, Methyl Red (MR) and Catalase positive. The growth rate of isolated strain was optimized by weighing dry cell and turbidity at 600 nm at different concentrations of dextrose (1%, 5% and 10%). Ten (10) percent dextrose solution showed rapid growth and higher cell mass than 5% and 1% solution respectively. Acidity of the media gradually increased from 0.102% to 2.18% from day 0 to day 7 and pH of the media decreased from 6.8 to 5.5 during the period. This protocol was successful for enriching Acetobacter aceti, which was essential for vinegar production. Keywords: Rotten Papaya, Acetobacter aceti, GYC culture media, Morphological and Biochemical assessment Introduction Acetic acid bacteria (AAB) is a group of gram-negative, aerobic and motile rods that carry out incomplete oxidation of alcohol and sugars, leading to the accumulation of organic acids as end products. -
Contemporary Pursuits of Vinegar from Scullery to Dermatology
International Journal of Research in Dermatology Bansal M et al. Int J Res Dermatol. 2020 Sep;6(5):708-714 http://www.ijord.com DOI: http://dx.doi.org/10.18203/issn.2455-4529.IntJResDermatol20203539 Review Article Contemporary pursuits of vinegar from scullery to dermatology Mansi Bansal1*, Umesh Budhiraja2, Himanshu Bansal3 1Department of Dermatology, Venereology, Leprosy, Kaya Skin Clinics, New Delhi, India 2Guru Gobind Singh Hospital, New Delhi, India 3PGIMS, Rohtak, Haryana, India Received: 01 August 2020 Accepted: 10 August 2020 *Correspondence: Dr. Mansi Bansal, E-mail: [email protected] Copyright: © the author(s), publisher and licensee Medip Academy. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Vinegar is widely available as a food ingredient for flavouring and as a preservative. It is one of the oldest skin remedy known to mankind. However, its status in treatment regimens has declined over the decades. This article is an attempt to highlight its therapeutic armamentarium in dermatology, venereology and leprosy. Acetic acid in vinegar has antibacterial, antifungal and antiviral properties. This review talks about various studies of acetic acid for various indications, such as screening for cervical cancer, healing of chronic wounds, atopic dermatitis, onychomycosis, marine dermatoses, acne vulgaris, warts, in sclerotherapy and many others dermatoses. Combination therapies and newer indications are also described in this article. Recently, its antiviral action in vitro has been demonstrated against the ongoing coronavirus disease of 2019 (COVID-19) pandemic. -
APPLE CIDER VINEGAR ~ How to Make from Scratch ~
APPLE CIDER VINEGAR ~ How to make from scratch ~ Apple Cider Quart Glass Jar &Plastic lid ¼ tsp. baker’s yeast ¼ cup “Mother of Vinegar” Vinegar: From the French “Vin-Aigre” meaning “sour wine” Fresh, sweet Cider turns to Hard cider cider - hard cider - turns to Contains yeast converts vinegar - natural or cider sugars acetobacter Step 1 Step 2 Step 3 Step added yeast; into alcohol; + oxygen - natural or acetobacter converts added (mother) alcohol into acetobacter introduced vinegar 1. Make or buy RAW apple cider. Raw cider contains natural yeast, the yeast in pasteurized juice has been killed (adding yeast is recommended for raw cider, required for pasteurized cider). The first step of turning cider to alcohol can be done by leaving the cider in the plastic jug it was purchased in or by pouring into a narrow-necked clean jar (smaller diameter neck helps prevent contamination). Cover with loosely fitting plastic wrap, plastic lid, or cheesecloth and rubber band (no metal!). Set in a clean, dark, warm place. 2. Yeast eats the sugar in fresh juice, converts to alcohol. Within a day to a week, the liquid will start to bubble (looks like champagne bubbles). As the bubble become more active, particles will float to the top and settle to the bottom. The smell should be of alcohol instead of sweet juice. You made hard cider – half way there! 3. Acetobacter convert alcohol to acetic acid (vinegar). Acetobacter (enemy to hard cider) are in the air, but to ensure a better transformation, you can add “mother of vinegar” to the cider alcohol as the bubbling starts to slow down. -
A Study of Some Chemical and Physical Characteristics of Vinegar Produced by the Malt of Some Varieties of Maize, Zehdi Dates, and Grapes
Journal of Natural Sciences Research www.iiste.org ISSN 2224-3186 (Paper) ISSN 2225-0921 (Online) Vol.6, No.6, 2016 A Study of Some Chemical and Physical Characteristics of Vinegar Produced by the Malt of Some Varieties of Maize, Zehdi Dates, and Grapes Qais Satwan Abass Abdullah Department of Horticulture & Gardens Engineering, College of Agriculture, Kirkuk University –Iraq Abstract This experiment was conducted for producing vinegar by varieties of maize, Zehdi dates and grapes and to study some of their chemical and physical properties. Also to assess some organic acids and mineral elements for samples. The experiment included five samples of vinegar produced by maize malt researches class (106) with symbol of (S1).Vinegar produced from spring class maize malt, with symbol of (S2).Vinegar produced from maize malt class (5012) with symbol of (S3).Vinegar produced from Zehdi dates, with symbol of (S4).Vinegar produced from grape, with symbol of (S5).The results showed the superiority of the properties of (S5) in (total acidity, alcohol percentage, moisture, total solids, total ash, relative density, viscosity), as the highest ratios of the properties were (4.46%, 0.53%, 96.107%, 4.3%, 0.52 %, 1.025 g / cm 3, 0.093 mli Boiz) except for the property of PH as the (S5) has recorded the highest values of (3.71) .Whereas (S3) has recorded the lowest in the properties of (total acidity, alcohol percentage, moisture content, total ash, relative density) as they have recorded (3.91%, 0.32%, 94.589%, 0.26%, 1.015 g / cm 3) respectively. (S1) on the other hand, has recorded less value of PH as it was (2.73), while (S4) has recorded the lowest value for TSS which was (2.9%), and (S2) recorded the lowest value for the relative viscosity which was (0.028) mli Boiz). -
PRODUCTION of ACETIC ACID from MOLASSES by FERMENTATION PROCESS Rutu Patel1, Hiral N
Vol-1 Issue-2 2015 IJARIIE-ISSN(O)-2395-4396 PRODUCTION OF ACETIC ACID FROM MOLASSES BY FERMENTATION PROCESS Rutu Patel1, Hiral N. Pandya2 1 Rutu Patel, Masters Fallow, Chemical Engineering Department, L.D.C.E., Gujarat, India. 2 Hiral N. Pandya, Assistant Professor, Chemical Engineering Department, L.D.C.E., Gujarat, India. ABSTRACT Acetic acid also called ethanoic acid is organic compound. Acetic acid produced via fermentation. Its pathway is conversion of glucose to ethanol and ethanol to acetic acid. In first step, Saccaromyces cerevesiae (yeast) converts fermentable sugar of molasses into ethanol and carbon dioxide. In second step, acetobacter aceti (acetic acid bacteria) converts ethanol into acetic acid and water. After completing process, the separation of product is carried out via centrifugation. Mixture of acetic acid and water is separated by distillation. In this report, details regarding cultures (micro-organism) have been used for the process is discussed. In practical laboratory work, ethanol fermentation and acetic acid fermentation have been carried out and it’s optimum parameters (pH, temperature, sugar concentration, and ethanol concentration) have been decided, which is discussed in detail. The kinetic study also have been done is mentioned. Key words: Saccaromyces cerevesiae, acetobacter aceti, micro-organism, fermentable sugar 1. FERMENTATION The term “fermentation” comes from a Latin word fermentum(to ferment). Fermentation is described as the process in which chemical changes occur in an organic substrate as the result of action of microbial enzymes. The product can either be: The cell itself: referred to as biomass production. A microorganisms own metabolite: referred to as a product from a natural or genetically improved strain. -
Developing the Optimal Vinaigrette Dressing for Managing Blood Glucose
Developing the Optimal Vinaigrette Dressing for Managing Blood Glucose Concentrations by Amber K. Bonsall A Thesis Presented in Partial Fulfillment of the Requirements for the Degree Master of Science Approved August 2016 by the Graduate Supervisory Committee: Carol Johnston, Chair Sandra Mayol-Kreiser Christy Lespron ARIZONA STATE UNIVERSITY May 2017 ABSTRACT Background: Acetic acid in vinegar has demonstrated antiglycemic effects in previous studies; however, the mechanism is unknown. Objective: To determine whether acetic acid dissociates in the addition of sodium chloride and describe a flavorful vinaigrette that maintains the functional properties of acetic acid. Design: Phase I - Ten healthy subjects (23-40 years) taste tested five homemade vinaigrette and five commercial dressings. Perceived saltiness, sweetness, tartness, and overall tasted were scored using a modified labeled affective magnitude scale. Each dressing was tested three times for pH with a calibrated meter. Phase II – Randomized crossover trial testing six dressings against a control dressing two groups of nine healthy adult subjects (18-52 years). Height, weight and calculated body mass index (BMI) were performed at baseline. Subjects participated in four test sessions each, at least seven days apart. After a 10-hour fast, participants consumed 38g of the test drink, followed by a bagel meal. Capillary blood glucose was obtained at fasting, and every 30 minutes over a 2-hour period the test meal. Results: Dressing pH reduced as sodium content increased. In the intervention trials, no significant differences were observed between groups (p >0.05). The greatest reduction in postprandial glycemia (~21%) was observed in the dressing containing 200 mg of sodium. -
Determination of Volatile Compounds in Fresh and Fermented Nipa Sap (Nypa Fruticans) Using Static Headspace Gas Chromatography-Mass Spectrometry (GC-MS)
International Food Research Journal 20(1): 369-376 (2013) Journal homepage: http://www.ifrj.upm.edu.my Determination of volatile compounds in fresh and fermented Nipa sap (Nypa fruticans) using static headspace gas chromatography-mass spectrometry (GC-MS) 1Nur Aimi, R., 1,2*Abu Bakar, F. and 1Dzulkifly, M. H. 1Halal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Malaysia 2Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Malaysia Article history Abstract Received: 5 April 2012 Nipa sap or air nira is a sweet natural beverage obtained from a type of palm tree, Nypa fruticans. Received in revised form: It is readily and spontaneously fermented resulting in the development of alcoholic fermentation 24 May 2012 products. Objective of this study is to determine the volatile compounds (VOCs) responsible Accepted: 25 May 2012 for the aroma in fresh and fermented nipa sap. The sap was left for natural fermentation at 30ºC for 63 days. VOCs of the sap were analysed using static headspace gas chromatography-mass spectrometry (GC-MS). Fresh nipa sap contained ethanol (83.43%), diacetyl (0.59%), and esters (15.97%). Fermented nipa sap contained alcohols (91.16 – 98.29%), esters (1.18 – 8.14%), acetoin (0.02 – 0.7%), diacetyl (0.04 – 0.06%), and acetic acid (0.13 – 0.68%). Concentration of ethanol in fresh nipa sap increased from 0.11% (v/v) to 6.63% (v/v) during the fermentation, Keywords and slightly decreased to 5.73% (v/v) at day 63. No higher alcohols were detected in the fresh nipa sap. -
Acetic Acid Bacteria – Perspectives of Application in Biotechnology – a Review
POLISH JOURNAL OF FOOD AND NUTRITION SCIENCES www.pan.olsztyn.pl/journal/ Pol. J. Food Nutr. Sci. e-mail: [email protected] 2009, Vol. 59, No. 1, pp. 17-23 ACETIC ACID BACTERIA – PERSPECTIVES OF APPLICATION IN BIOTECHNOLOGY – A REVIEW Lidia Stasiak, Stanisław Błażejak Department of Food Biotechnology and Microbiology, Warsaw University of Life Science, Warsaw, Poland Key words: acetic acid bacteria, Gluconacetobacter xylinus, glycerol, dihydroxyacetone, biotransformation The most commonly recognized and utilized characteristics of acetic acid bacteria is their capacity for oxidizing ethanol to acetic acid. Those microorganisms are a source of other valuable compounds, including among others cellulose, gluconic acid and dihydroxyacetone. A number of inves- tigations have recently been conducted into the optimization of the process of glycerol biotransformation into dihydroxyacetone (DHA) with the use of acetic acid bacteria of the species Gluconobacter and Acetobacter. DHA is observed to be increasingly employed in dermatology, medicine and cosmetics. The manuscript addresses pathways of synthesis of that compound and an overview of methods that enable increasing the effectiveness of glycerol transformation into dihydroxyacetone. INTRODUCTION glucose to acetic acid [Yamada & Yukphan, 2007]. Another genus, Acetomonas, was described in the year 1954. In turn, Multiple species of acetic acid bacteria are capable of in- in the year 1984, Acetobacter was divided into two sub-genera: complete oxidation of carbohydrates and alcohols to alde- Acetobacter and Gluconoacetobacter, yet the year 1998 brought hydes, ketones and organic acids [Matsushita et al., 2003; another change in the taxonomy and Gluconacetobacter was Deppenmeier et al., 2002]. Oxidation products are secreted recognized as a separate genus [Yamada & Yukphan, 2007].