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International 300 N INFORMATION TO USERS This reproduction was made from a copy of a document sent to us for microfilming. While the most advanced technology has been used to photograph and reproduce this document, the quality of the reproduction is heavily dependent upon the quality of the material submitted. The following explanation of techniques is provided to help clarify markings or notations which may appear on this reproduction. 1.The sign or “ target” for pages apparently lacking from the document photographed is “ Missing Page(s)” . I f it was possible to obtain the missing page(s) or section, they are spliced into the film along with adjacent pages. This may have necessitated cutting through an image and duplicating adjacent pages to assure complete continuity. 2. When an image on the film is obliterated with a round black mark, it is an indication of either blurred copy because of movement during exposure, duplicate copy, or copyrighted materials that should not have been filmed. For blurred pages, a good image of the page can be found in the adjacent frame. If copyrighted materials were deleted, a target note will appear listing the pages in the adjacent frame. 3. When a map, drawing or chart, etc., is part of the material being photographed, a definite method of “sectioning” the material has been followed. It is customary to begin film ing at the upper left hand corner o f a large sheet and to continue from left to right in equal sections with small overlaps. I f necessary, sectioning is continued again—beginning below the first row and continuing on until complete. 4. For illustrations that cannot be satisfactorily reproduced by xerographic means, photographic prints can be purchased at additional cost and inserted into your xerographic copy. These prints are available upon request from the Dissertations Customer Services Department. 5. Some pages in any document may have indistinct print. In all cases the best available copy has been filmed. University M icrofilm s International 300 N. Zeeb Road Ann Arbor, Ml 48106 8305355 Liuzzi, Francis Joseph THE DEVELOPMENT OF DORSAL ROOT AFFERENTS AND THE LATERAL MOTOR COLUMN IN THE BULLFROG LUMBAR ENLARGEMENT AS SHOWN BY HRP INJURY FILLING OF DORSAL AND VENTRAL ROOTS The Ohio State University PH.D. 1982 University Microfilms International 300 N. Zeeb Road, Ann Arbor, M I 48106 PLEASE NOTE: In all cases this material has been filmed in the best possible way from the available copy. Problems encountered with this document have been identified here with a checkV mark. 1. Glossy photographs or pages 2. Colored illustrations, paper or print____ 3. Photographs with dark background ^ 4. Illustrations are poor copy______ 5. Pages with black marks, not original copy__ 6. Print shows through as there is text on both sides of page______ 7. Indistinct, broken or small print on several pages___ 8. Print exceeds margin requirements_____ 9. Tightly bound copy with print lost in spine______ 10. Computer printout pages with indistinct print______ 11. Page(s)____________lacking when material received, and not available from school or author. 12. Page(s)____________seem to be missing in numbering only as text follows. 13. Two pages numbered_____________. Text follows. 14. Curling and wrinkled pages______ 15. Other_________________________________________________________________________ University Microfilms International THE DEVELOPMENT OF DORSAL ROOT AFFERENTS AND THE LATERAL MOTOR COLUMN IN THE BULLFROG LUMBAR ENLARGEMENT AS SHOWN BY HRP INJURY FILLING OF DORSAL AND VENTRAL ROOTS. DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of the Ohio State University By Francis J. Liuzzi, B.S., M.S. ***** The Ohio State University 1982 Reading Committee: Approved by Michael S. Beattie, Ph.D. Co-Adviser Jacqueline C. Bresnahan, Ph.D. Co-Adviser George F. Martin, Ph.D. James S. King, Ph.D. Adviser Adviser Department of Anato For Rosemary, Cisco and Guy ii ACKNOWLEDGMENTS I wish to thank Dr. Michael S. Beattie and Dr. Jacqueline C. Bresnahan for allowing me to be a part of their laboratory and for their encouragement throughout my graduate career. I am indebted to Tina Hatten Van Meter for her technical expertise and assistance. Karl Rubin not only provided technical advice but close friendship that made my tenure at Ohio State more pleasant and at times fun. I would also like to thank all of the faculty of the Department of Anatomy, especially Dr. James S. King and Dr. George F. Martin, for their knowledge, advice and encouragement. Finally, I want to thank my wife Rosemary and my sons Cisco and Guy for their love and patience. This degree is as much theirs as it is my own. VITA NAME: Francis Joseph Liuzzi DATE OF BIRTH: October 4, 1949 PLACE OF BIRTH: Albany, New York EDUCATION: Grade School and High School P.S. 25, Albany, N.Y. 1954-1958 Saddlewood Elementary School, Colonie, N.Y. 1959-1961 Lisha Kill Junior High School, Colonie, N.Y. 1962-1963 Colonie Cenfral High School, Colonie, N.Y. 1964-1967 College and Degrees Union College, Schenectady, N.Y. B.S. Psychology 1971 Rensselaer Polytechnic Institute, Troy, N.Y. M.S. Psychology 1975 Ohio State University, Columbus, Ohio PhD. Anatomy 1982 TEACHING EXPERIENCE: Introductory Psychology, 1972, 1973 Physiological Psychology Labora­ tory, 1973 Electron Microscopy for Histo- technologists, 1975-1979 Gross Anatomy for Dental Students, 1980 Neuroanatomy for Dental Students, 1981 Neuroanatomy for Medical Students, 1981 iv RESEARCH TRAINING: Synaptogenesis of axosomatic synapse of primary afferents on motor neurons in the ventral horn of ranid tadpoles as shown by HRP injury filling. Comparative organization of primary afferents as shown by HRP root labelling. SCIENTIFIC SOCIETIES: Electron Microscopic Society of America Society for Neuroscience HONORS: Presidential Fellowship - The Ohio State University - 1981-1982 ABSTRACTS AND PUBLICATIONS: Beattie, M.S., Bresnahan, J.C. , Ho, R.H. and Liuzzi, F. , 1979 "Distribution of met-enkephalin, substance-P and soma­ tostatin in the spinal cord dorsal horn after hemisection in the rat." Neurosci. Absts., 5, 719. Beattie, M.S., Bresnahan, J.C. and Liuzzi, F. 1980, "Ultra­ structure of dorsal root afferents to the ventral horn of the cat." Neurosci. Absts., 6 , 435. Beattie, M.S., Bresnahan, J.C. and Liuzzi, F. 1981, "Aberrant dorsal root axons in the dorsal horn after chronic spinal hemisection in the cat." Pain, Abstracts of the Third World Congress on Pain, Suppl. 11-319, S239. Beattie, M.S., Bresnahan, J.C. and Liuzzi, F., 1981, "Aberrant axons from the dorsal roots after chronic spinal hemi­ section in the cat." Neurosci. Absts., in press. Liuzzi, F.J., Beattie, M.S. and Bresnahan, J.C. , "Development of the relationship between dorsal root afferents and the lateral motor column of the bullfrog spinal cord," Neurosci. Absts., in press. Beattie, M.S., Liuzzi, F.J., and Bresnahan, J.C., "Migrating motoneurons and their relationship to developing dorsal root afferents in the bullfrog tadpole spinal cord," Neurosci. Absts. in press. Campbell, H.L., Liuzzi, F.J., Beattie, M.S., and Bresnahan, J.C., "Large circumferential cells of the developing Rana catesbeiana spinal cord are labelled after HRP application to lateral funiculus, " Neurosci. Absts., in press. v Liuzzi, Francis J., Beattie, Michael S. and Bresnahan, Jacqueline C., 1982, "Development of the monosynaptic reflex arc in the lumbar spinal cord of Rana catesbeiana as shown by simultaneous labelling of dorsal and ventral roots with HRP," Anat. Rec.. 202, 113A. Liuzzi, F.J., Beattie, M.S. and Bresnahan, J.C., "Dorsal root afferents contact migrating motoneurons," submitted 1982. TABLE OF CONTENTS Page DEDICATION .................................................... ii ACKNOWLEDGMENTS ................................... iii VITA .......................................................... iv LIST OF FIGURES .............................................. ix INTRODUCTION .................................................. 1 MATERIAL AND METHODS ......................................... 5 Subjects ................................................. 5 Surgery ................................................. 5 Tissue Preparation ...................................... 6 Analysis ................................................. 7 RESULTS: ADULT FROG ......................................... 9 Primary Afferent Distribution............................. 9 LMC Motoneuron Morphology ........ 10 Primary Afferent-LMC Interactions ..................... 11 Electron Microscopic Observations ..................... 12 RESULTS: LARVAL FROG ........................................ 14 FOOT PADDLE STAGES ........................................ 14 Primary Afferents ....................................... 14 LMC Motoneurons ........................................ 15 Primary Afferent-LMC Motoneuron Interactions .......... 16 FOOT OR PREMETAMORPHIC STAGES ............................. 17 Primary Afferents ................................ 17 LMC Motoneuron Morphology ..................... 19 Primary Afferent-LMC Motoneuron Interactions .......... 20 Electron Microscopic Observations ..................... 22 vii Page MIGRATING LMC MOTONEURONS ................................... 22 DISCUSSION .................................................... 26 Primary Afferent Distribution in Adult Frog ........... 26 The LMC in the Adult Frog .............................. 31 Development of Primary Afferents ...................... 33 Development
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