Table S1. Proteins identified in 1D proteomic screen of metaphase II-arrested mouse oocytes.
Band Number Factor MGI number MW
Ubiquitination and Protein degradation 1 1 Skp1a (S-phase kinase-associated protein 1A) MGI:103575 18.6 2 1 Ubiquitin na 11.7 3 1 Ube2d2 (ubiquitin-conjugating enzyme E2D 2) MGI:1930715 13.1 4 3 Uchl1 (ubiquitin carboxy-terminal hydrolase L1) MGI:103149 24.8 5 4 Ube2k (ubiquitin-conjugating enzyme E2K) MGI:1858216 22.4 6 6 March5 (membrane-associated ring finger 5) MGI:1915207 27.3 7 7 Ube2s (ubiquitin-conjugating enzyme E2S) MGI:1925141 23.8 8 8 Dcun1d1 (DCN1) MGI:2150386 30.1 9 14 Otub1 (OTU domain, ubiquitin aldehyde binding 1) MGI:2147616 43.3 10 18 Sugt1 (SGT1, suppressor of G2 allele of SKP1) MGI:1915205 30.0 11 20 Fbxw14 (F-box and WD-40 domain protein 14) MGI:1354703 54.3 12 22 Fbxw13 (F-box and WD-40 domain protein 13) MGI:3505590 53.9 13 25 Cops2 (COP9 (constitutive photomorphogenic) homolog, subunit 2) MGI:1330276 52.5 14 39 Cul1 (cullin 1) MGI:1349658 89.9 15 40 Cul4b (cullin 4B) MGI:1919834 71.7 16 45 Uba1 (ubiquitin-like modifier activating enzyme 1) MGI:98890 118 Oxidation/Reduction 17 1 Prdx5 (peroxiredoxin 5) MGI:1859821 21.9 18 1 Sod1 (superoxide dismutase 1) MGI:98351 15.9 19 1 Ggct (gamma-glutamyl cyclotransferase) MGI:95700 21.1 20 1 Mgst3 (microsomal glutathione S-transferase 3) MGI:1913697 17.0 21 1 Glrx (glutaredoxin) MGI:2135625 18.1 22 3 Prdx2 (peroxiredoxin 2) MGI:109486 21.7 23 4 Gstp1 (glutathione S-transferase, pi 1) MGI:95865 23.5 24 4 Prdx1 (peroxiredoxin 1) MGI:99523 22.1 25 4 Prdx3 (peroxiredoxin 3) MGI:88034 28.1 26 11 Nudt5 (nudix) MGI:1858232 24.0 27 15 Akr1b3 (aldose reductase) MGI:1353494 35.7 28 17 Akr7a5 (aflatoxin aldehyde reductase) MGI:107796 41.0 29 18 Glrx3 (glutaredoxin 3) MGI:1353653 29.4 30 23 Txndc5 (thioredoxin domain containing 5) MGI:2145316 27.3 31 24 Pdia6 (protein disulfide isomerase associated 6) MGI:1919103 48.1 32 27 Dld (dihydrolipoamide dehydrogenase) MGI:107450 54.3 33 28, 29 Pdia3 (protein disulfide isomerase associated 3) MGI:95834 56.7 34 30 P4hb (prolyl 4-hydroxylase, beta polypeptide) MGI:97464 57.2 35 36 Pdia4 (protein disulfide isomerase associated 4) MGI:104864 72.0 RNA binding/metabolism/transport 36 1 Ooep (MOEP19) MGI:1915218 18.4 37 1 Dppa5a (developmental pluripotency associated 5A, Esg1) MGI:101800 13.7 38 16 Oas1e (2'-5' oligoadenylate synthetase 1E) MGI:2180856 41.9 39 18 Oas1d (2'-5' oligoadenylate synthetase 1D) MGI:2140770 33.7 40 27 Ddx6 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 6) MGI:104976 51.1 41 27 Ybx2 (MSY2) MGI:1096372 31.5 42 28 Ddx19a (DEAD (Asp-Glu-Ala-Asp) box polypeptide 19a) MGI:99526 54.0 43 32 G3bp2 (GTPase activating protein (SH3 domain) binding protein 2) MGI:2442040 54.1 44 37 Ddx3x (DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked) MGI:103064 73.2 45 44 Mvp (major vault protein) MGI:1925638 95.9 Transport 46 1 Arf1 (ADP-ribosylation factor 1) MGI:99431 20.7 47 2 Rab2A (RAB2A) MGI:1928750 23.5 48 3 Rab7 (RAB7) MGI:105068 23.5 49 4 Rab1 (RAB1) MGI:97842 22.7 50 4 Rab11b (RAB11B) MGI:99425 24.5 51 4 Rab14 (RAB14) MGI:1915615 23.9 52 4 Rab5b (RAB5B) MGI:105938 23.7 53 4 Arhgdia (Rho GDP dissociation inhibitor (GDI) alpha) MGI:2178103 23.4 54 5 Ran (RAN) MGI:1333112 24.4 55 7 Gbas (glioblastoma amplified sequence) MGI:1278343 32.9 56 12 Cyb5r3 (cytochrome b5 reductase 3) MGI:94893 34.1 57 14 Cope (coatomer protein complex, subunit epsilon) MGI:1891702 16.3 58 17 Vps26a (vacuolar protein sorting 26 homolog A) MGI:1353654 38.1 59 22 Anxa7 (annexin A7) MGI:88031 41.3 60 23 Gdi1 (Rab GDIalpha) MGI:99846 36.7 61 24 Gdi2 (GDI beta) MGI:99845 41.8 62 30 Kpna2 (karyopherin (importin) alpha 2) MGI:103561 57.8 63 37 Rdx (radixin) MGI:97887 68.5 64 37 Nsf (N-ethylmaleimide sensitive fusion protein) MGI:104560 82.7 65 42 Vcp (valosin containing protein) MGI:99919 89.4 66 51 Myh9 (myosin, heavy polypeptide 9, non-muscle) MGI:107717 226 67 52 Dync1h1 (dynein cytoplasmic 1 heavy chain 1) MGI:103147 532 Cytoskeleton 68 1 Pfn1 (profilin 1) MGI:97549 14.9 69 1 Cfl1 (cofilin 1) MGI:101757 18.5
1 Table S1. Proteins identified in 1D proteomic screen of metaphase II-arrested mouse oocytes.
70 1 Cfl2 (cofilin 2) MGI:101763 18.7 71 2 Acta2 (smooth muscle actin, alpha 2) MGI:87909 42.0 72 2 Cdc42 (cell division cycle 42 homolog) MGI:106211 21.2 73 8 Tuba1a (tubulin, alpha 1A) MGI:98869 50.1 74 18 Actg1 (actin, gamma, cytoplasmic 1) MGI:87906 17.7 75 23 Pafah1b1 (platelet-activating factor acetylhydrolase) MGI:109520 37.3 76 23 Tubb6 (tubulin, beta 6) MGI:1915201 15.3 77 24 Tubb5 (tubulin, beta 5) MGI:107812 49.7 78 36 Ezr (ezrin) MGI:98931 69.5 79 41 Efha1 (EF hand domain family A1) MGI:1915764 49.5 Transcription 80 1 Polr2d (polymerase (RNA) II (DNA directed) polypeptide D) MGI:1916491 12.2 81 34 Tle6 (transducin-like enhancer of split 6, homolog of Drosophila E(spl)) MGI:2149593 65.1 82 45 Uhrf1 (ubiquitin-like, containing PHD and RING finger domains, 1) MGI:1338889 88.2 83 45 Dnmt1 (DNA methyltransferase (cytosine-5) 1) MGI:94912 183 Translation 84 1 Rps10 (ribosomal protein S10) MGI:1914347 18.8 85 2 Rpl11 (ribosomal protein L11) MGI:1914275 20.2 86 11 Rpl7 (ribosomal protein L7) MGI:98073 31.4 87 13 Naca (nascent polypeptide-associated complex alpha polypeptide) MGI:106095 23.4 88 17 Eef1d (eukaryotic translation elongation factor 1 delta) MGI:1913906 31.3 89 17 Eef1a2 (eukaryotic translation elongation factor 1 alpha 2) MGI:1096317 50.4 90 21 Eef1a1 (eukaryotic translation elongation factor 1 alpha 1) MGI:1096881 50.2 91 37 Gars (glycyl-tRNA synthetase) MGI:2449057 82.0 92 42 Eef2 (eukaryotic translation elongation factor 2) MGI:95288 95.4 Cell Cycle 93 2 Spin1 (spindlin 1) MGI:109242 27.1 Mitochondrial 94 1 Tmem14c (transmembrane protein 14C) MGI:1913404 10.8 95 8 Echs1 (enoyl Coenzyme A hydratase) MGI:2136460 31.2 96 8 Ak2 (adenylate kinase 2) MGI:87978 25.6 97 9 Slc25a5 (adenine nucleotide translocase) MGI:1353496 32.9 98 10 Vdac3 (voltage-dependent anion channel 3) MGI:106922 30.7 99 14 Mdh2 (malate dehydrogenase 2) MGI:97050 35.6 100 20 Gcat (glycine C-acetyltransferase) MGI:1349389 44.9 101 27 Atp5a1 (ATP synthase) MGI:88115 59.8 Metabolism 102 1 Ppia (peptidylprolyl isomerase A) MGI:97749 17.9 103 1 Ldhb (lactate dehydrogenase B) MGI:96763 36.5 104 2 Ptges3 (prostaglandin E synthase 3) MGI:1929282 18.7 105 3 Pebp1 (phosphatidylethanolamine binding protein 1) MGI:1344408 20.8 106 3 Dcakd (dephospho-CoA kinase domain containing) MGI:1915337 26.5 107 4 Gstp1 MGI:95865 23.5 108 6 Gstm5 (glutathione S-transferase, mu 5) MGI:1309466 26.7 109 7 Hsd17b10 (hydroxysteroid (17-beta) dehydrogenase 10) MGI:1333871 27.4 110 9 Miox (myo-inositol oxygenase) MGI:1891725 33.1 111 9 Tpmt (thiopurine methyltransferase) MGI:98812 27.6 112 9 Pycrl (pyrroline-5-carboxylate reductase-like) MGI:1913444 28.7 113 9 Bpgm (2,3-bisphosphoglycerate mutase) MGI:1098242 29.9 114 11 Gnpda2 (glucosamine-6-phosphate deaminase 2) MGI:1915230 31.1 115 11 Pecr (peroxisomal trans-2-enoyl-CoA reductase) MGI:2148199 32.3 116 11 Degs1 (degenerative spermatocyte homolog 1) MGI:1097711 38.2 117 11 Pkm2 (pyruvate kinase, muscle) MGI:97591 57.8 118 12 Ldhc (lactate dehydrogenase C) MGI:96764 35.8 119 14 Ppa1 (pyrophosphatase) MGI:97831 32.6 120 15 Gdpd1 (glycerophosphodiester phosphodiesterase domain containing 1) MGI:1913819 38.7 121 15 Gapdh (glyceraldehyde-3-phosphate dehydrogenase) MGI:95640 35.8 122 15 Mdh1 (malate dehydrogenase 1, NAD (soluble)) MGI:97051 36.5 123 16 Akr1a4 (aldehyde reductase) MGI:1929955 35.7 124 16 Cryl1 (crystallin, lambda 1) MGI:1915881 27.0 125 17 Mecr (mitochondrial trans-2-enoyl-CoA reductase) MGI:1349441 40.3 126 17 Idh3a (isocitrate dehydrogenase 3 (NAD+) alpha) MGI:1915084 40.0 127 17 Arg2 (arginase type II) MGI:1330806 38.9 128 17 Adprh (ADP-ribosylarginine hydrolase) MGI:1098234 40.1 129 17 Aldoa (aldolase A, fructose-bisphosphate) MGI:87994 39.5 130 18, 33 Pla2g4c (CPLA2-gamma ) MGI:1196403 67.9 131 20 Ckb (creatine kinase) MGI:88407 34.1 132 21 Idh1 (isocitrate dehydrogenase 1) MGI:96413 37.8 133 22 Ahcy (S-adenosylhomocysteine hydrolase) MGI:87968 39.1 134 22 Cs (citrate synthase) MGI:88529 43.4 135 23 Adss (adenylosuccinate synthetase) MGI:87948 41.9 136 24 Eno1 (enolase 1, alpha non-neuron) MGI:95393 47.2 137 25 Aldh9a1 (aldehyde dehydrogenase 9, subfamily A1) MGI:1861622 53.6 138 25 Aldh2 (aldehyde dehydrogenase 2, mitochondrial) MGI:99600 56.6 139 25 Dlst (dihydrolipoamide S-succinyltransferase) MGI:1926170 49.0 140 27 Aldh7a1 (aldehyde dehydrogenase family 7, member A1) MGI:108186 55.6 141 28 G6pdx (glucose-6-phosphate dehydrogenase X-linked) MGI:105979 59.3
2 Table S1. Proteins identified in 1D proteomic screen of metaphase II-arrested mouse oocytes.
142 28 Gpi1 (glucose phosphate isomerase 1) MGI:95797 62.8 143 29 Paox (polyamine oxidase (exo-N4-amino)) MGI:1916983 55.4 144 34 Acox3 (acyl-Coenzyme A oxidase 3, pristanoyl) MGI:1933156 78.5 145 37 Thop1 (thimet oligopeptidase 1) MGI:1354165 80.0 Signal transduction 146 2 Ywhaz(14-3-3 protein zeta/delta) MGI:109484 27.7 147 9 Ywhag (14-3-3 protein gamma) MGI:108109 28.3 148 9 Ywhaq (14-3-3 protein tau/theta) MGI:891963 27.7 149 9 Ywhah (14-3-3 protein eta) MGI:109194 28.2 150 10 Ywhae (14-3-3 protein epsilon) MGI:894689 29.1 151 11 Zbed3 (zinc finger, BED domain containing 3) MGI:1919364 25.6 152 12 Gnb2l1 (Rack1) MGI:101849 35.0 153 14 Rgn (regucalcin) MGI:108024 33.4 154 37 Plaa (phospholipase A2, activating protein) MGI:104810 71.5 DNA repair 155 12 Pcna (proliferating cell nuclear antigen) MGI:97503 28.9 156 46 Ddb1 (damage specific DNA binding protein 1) MGI:1202384 127 Chaperone 157 8 Erp29 (endoplasmic reticulum protein 29) MGI:1914647 28.8 158 21 Hsp90aa1 (heat shock protein 90) MGI:96250 84.8 159 25 St13 (suppression of tumorigenicity 13) MGI:1917606 41.7 160 27 Calr (calreticulin) MGI:88252 48.0 161 28 Cct2 (chaperonin containing Tcp1, subunit 2 (beta)) MGI:107186 57.5 162 30 Cct8 (chaperonin containing Tcp1, subunit 8 (theta)) MGI:107183 59.6 163 30 Cct7 (chaperonin containing Tcp1, subunit 7 (eta)) MGI:107184 59.7 164 31 Hspd1 (heat shock protein 1 (chaperonin)) MGI:96242 61.0 165 31 Tcp1 (t-complex protein 1) MGI:98535 60.5 166 31 Cct6a (chaperonin containing Tcp1, subunit 6a (zeta)) MGI:107943 58.1 167 32 Cct3 (chaperonin containing Tcp1, subunit 3 (gamma)) MGI:104708 60.5 168 32 Stip1 (stress-induced phosphoprotein 1) MGI:109130 62.6 169 32 Cct5 (chaperonin containing Tcp1, subunit 5 (epsilon)) MGI:107185 59.7 170 36 Hspa5 (heat shock protein 5) MGI:95835 72.5 171 39 Canx (calnexin) MGI:88261 67.3 172 39 Hsp90ab1 (heat shock protein 90 alpha (cytosolic), class B member 1) MGI:96247 83.4 173 40 Trap1 (TNF receptor-associated protein 1) MGI:1915265 80.3 174 45 Hsph1 (heat shock 105kDa/110kDa protein 1) MGI:105053 96.3 175 45 Hsp90b1 (heat shock protein 90, beta (Grp94), member 1) MGI:98817 92.4 176 47 Hyou1 (hypoxia up-regulated 1) MGI:108030 72.2 Post-Translational Modification 177 1 Nat13 (N-acetyltransferase 13) MGI:1919367 19.3 178 3 Dcakd (dephospho-CoA kinase domain containing) MGI:1915337 26.5 179 32 Rpn2 (ribophorin II) MGI:98085 69.1 180 35 Mtmr14 (myotubularin related protein 14) MGI:1916075 59.3 Other 181 8 2410004A20Rik (Filia) MGI:1914241 48.0 182 24 EG382106 (predicted gene, EG382106) MGI:3646659 na 183 34 PADI6 (peptidylarginine deiminase 6) MGI:2655198 75.0 184 45 Nlrp14 (NLR family, pyrin domain containing 14) MGI:1924108 75.4 185 45 Nlrp5 (MATER, NLR family, pyrin domain containing 5) MGI:1345193 125
3