Catalog # Aliquot Size

E318-911-05 3 x 5 nmol E318-911-20 3 x 20 nmol E318-911-50 3 x 50 nmol

EHMT2 siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # E318-911

Lot # Z2037-9

Specificity Formulation EHMT2 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human EHMT2 expression. shipped at room temperature.

Product Description Reconstitution Protocol EHMT2 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human EHMT2 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_006709. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot G9A, NG36, NG36/G9a 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or EHMT2, Active E318-381G below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background EHMT2 is a -preferring histone methyltransferase. EHMT2 siRNA Set I EHMT2 can methylate itself at K165 and this siRNA duplexes targeted against three exon regions automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 Catalog Number E318-911 (HP1) (1). EHMT2 is responsible for the majority of Specific Lot Number Z2037-9 dimethylation of at lysine 9 () and is Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol required for the efficient repression of developmentally Format Lyophilized powder regulated during embryonic stem cell Stability 1yr at -70oC from date of shipment differentiation (2). EHMT2 is overexpressed in various Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is cancers and can can specifically methylate p53 at recommended that the lyophilized Lys(373). EHMT2 polymorphisms have been associated and resuspended siRNAs are stored at with colorectal cancer. or below -20oC. After resuspension, siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles References without significant degradation. For 1. Sampath, S C. et al: Methylation of a histone mimic long-term storage, it is recommended that the siRNA is stored at -70oC. For within the histone methyltransferase G9a regulates most favorable performance, avoid protein complex assembly. Molec. Cell 27: 596-608, repeated handling and multiple 2007. freeze/thaw cycles. 2. Lehnertz B. et al: Activating and inhibitory functions for the histone lysine methyltransferase G9a in T helper cell differentiation and function. J Exp Med. 2010 May 10;207(5):915-22.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.