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LINZ 2018 – EUSAAT 2018 Volume 7, No LINZ 2018 – EUSAAT 2018 Volume 7, No. 2 ISSN 2194-0479 (2018) ALTEXProceedings Welcome In Silico Models: Toxicology & Efficacy 3D Models & Multi-Organ- of Drugs, Chips (MOC), Chemicals & Cosmetics Human-Organ-Chips (HOC) International Progress 3R Centers in Europe in 3Rs Research 3Rs in Education In Vitro Techniques and Academia for CNS Toxicity and Disease Studies Advanced Safety Testing of Cosmetics and Refinement & Reduction Consumer Products Replacement – Advanced Alternatives to Animal Technologies for Testing in Food Safety, Implementation of 3Rs Nutrition and Efficacy Specific Endpoints Biological Barriers LINZ 2018 of Toxicity Disease Models 21st European Congress Stem Cell Models Using Human Cells, on Alternatives to Animal Testing (hiPS, ES, mES, miPS) Tissues and Organs EUSAAT 2018 Update on Implementing Ecotoxicology 18th Annual Congress of EUSAAT EU Dir 63/2010 Efficacy and Safety Vaccines & The 3Rs: Testing of Drugs, New Methods and Medical Devices & www.eusaat-congress.eu Developments (e.g. Batch Biopharmaceutics Release Testing) Free Communications 'Young Scientists' session The world leader in innovative 3D reconstructed human tissue models ISO 9001 CERTIFIED KEY FEATURES OF OUR PRODUCTS TISSUE MODELS AVAILABLE FROM SLOVAKIA AND USA Metabolically active, human cell-derived, 3D reconstructed tissue models EpiDerm™ Objective, quantifiable endpoints Skin Corrosion, Skin Irritation, Phototoxicity, Genotoxicity Excellent in vitro / in vivo correlation Micronucleus and Comet assay, Medical devices, Skin Guaranteed long-term reproducibility Inflammation, Skin metabolism, Radiation Damage, Availability of partial and full thickness tissue models Percutaneous Absorption … Availability of different tissue formats including high throughput screening plate Price convenient testing kits as well as single tissues EpiOcular™ Ocular Irritation, “Sub-Draize” Ocular Irritation Testing (Mild, Milder, Mildest), Specific Ocular Irritation of Surfactants, EXCELLENT CUSTOMER SERVICE Cosmetics and Consumer Products … AND PROFESSIONAL SCIENTIFIC SUPPORT EpiIntestinal™ Prompt reply to customer´s requests and needs Intestinal Toxicity, Drug Delivery, Inflammation, Fibrosis, Infection, Free scientific and technical consulting before purchasing of product Epithelial Restitution … Monitoring of every single shipment Dedicated trainings sessions in our facilities or on-site TISSUE MODELS AVAILABLE FROM USA International support provided by EU, US, Turkey and Japan representatives More than 20 years of tissue quality control data summarized in open databases - the most data of any tissue engineering company ! EpiDermFT™ Since 2016 ISO 9001:2008 certified Anti-Aging, Skin Longevity, Skin Hydration, UV Protection, Percutaneous Absorption … INTERNATIONAL VALIDATIONS AND PROJECTS EpiCorneal™ Drug Delivery, Infection, Inflammation, Ophthalmic Product EpiDerm™ Testing … ECVAM Skin Corrosion, Validated Assay - OECD TG 431 ECVAM Skin Irritation, Validated Assay - OECD TG 439 EpiOral™ and EpiGingival™ ECVAM Pre-Validated and ICH Accepted Phototoxicity Assay Mucosal and Oral Irritation, Transmucosal and Buccal Drug Cosmetics Europe Validation Project on Genotoxicity Assays Delivery, Gum Disease, Oral Cancer, Smokeless Tobacco German Skin Penetration Validation Study for Surfactants and Formulations Effects, Oral Epithelial Proliferation, Antimicrobial Barrier Irritation Potency of Extracts from Medical Devices Study (ISO 10993-10) Function … EpiOcular™ EpiAirway™ ECVAM/Cosmetics Europe Eye Irritation, Validated Assay - OECD TG 492 Drug Delivery, Respiratory Infection and Toxicology, Tobacco US EPA Accepted for Antimicrobial Products with Cleaning Claims (AMCPs) Smoke Toxicology, Nano-particle Toxicology, Gene Expression COLGATE/IIVS Eye Irritation Validation Study Analysis, RNAi/siRNA Therapeutic Drug … Con4Eye Project on Eye Irritation Testing Strategies MelanoDerm™ EpiVaginal™ Skin Lightening-Darkening, Skin Pigmentation Modulation, NIH Funded HIV Research Keratinocyte-Melanocyte Interactions … CONRAD Microbicides Study Psoriasis™ AFFILIATIONS AND MEMBERSHIP IN PROFESSIONAL OR- Anti-Psoriatic Drug Screening, Basic Psoriasis Research ... GANISATIONS AND CONSORTIA IN THE EU EpiVaginal™ Feminine Product Irritation, Microbicide Testing, Immuno- competent, HIV-1 Infection Sexually Transmitted Infection, Inflammation, Vaginal Drug Delivery … Melanoma™ Tumor Invasion, Anti-Melanoma Drug Screening ... PRIMARY CELLS & MEDIA FROM USA Primary human cells derived from human tissue for high throughput screening Your trustworthy partner in the world of in vitro science for the past 30 years MatTek In Vitro Life Science Laboratories MatTek Corporation Mlynské Nivy 73, 821 05 Bratislava, Slovakia, EU 200 Homer Avenue, Ashland, MA 01721, USA Phone: +421-2-3260-7408 Phone: +1-508-881-6771 Fax: +421-2-3260-7404 Fax: +1-508-879-1532 E-mail: [email protected] E-mail: [email protected] Web: www.mattek.com Web: www.mattek.com A4 MatTek leaflet v12.1 - MKver9-2017.indd 1 7. 3. 2018 7:23:43 ABSTRACTS LINZ 2018 Welcome address EUSAAT 2018 Dear friends and colleagues, on behalf of EUSAAT, the European Society for Alternatives to Animal Testing, we welcome you to the 21st European Congress on Alternatives to Animal Testing & the 18th EUSAAT 3Rs congress in Linz, Austria on September 23-26, 2018. During the past two decades the “Linz-Congress” has emerged in Europe as the major scientific event in the field of the 3Rs and in the year 2018 the EUSAAT 2018 3Rs Congress Linz will be the largest international 3Rs congress. We are particularly pleased that our international partner societies from China, CCARE, the Chinese Society for Alternatives to Animal Experiments, and from the USA, ASCCT, the American Society for Cellular and Computational Toxicology, are actively participating in our session on “International Progress in 3Rs Research”. EUSAAT 2018 is hosting oral and poster presentations to facilitate discussions and the exchange of ideas for the benefit of alternative methods to animal experiments. The Scientific Committee has identified the most important subjects related to the 3Rs that are currently of interest to scientists in Europe. The EUSAAT 2018 conference focuses in oral and poster sessions on Refinement and Reduction (e.g. animal welfare, education) as well as Replacement topics (e.g. disease models, non-animal tools for basic biomedical research, toxicity studies, omics techniques, advanced 3D models including recent progress in developing human-organ-chips). After the success in 2016, this year we will host three round table discussions on topics that are of particular interest to EUSAAT members. The first on “International Progress in 3Rs Research”, the second on “3R centers in Europe – National and Local Centers” and the third on “Applying human 3D models and Multi-Organ-Chips (MOCs) in Industry”. We are grateful to all speakers for contributing to the training workshop “EUSAAT - Kirkstall Quasi Vivo® Training Workshop” and in particular to the company Kirkstall for the organization of this workshop, which is open to all partici- pants of the EUSAAT 2018 congress. In particular, we are very proud that we are able to continue the “Young Scientists Travel Award (YSTA)” program in 2018 and want to thank the SET Foundation for their generous funding. The YSTA program provides about 20 young scientists with the opportunity to share their ideas on the implementation of the 3Rs in education and novel test systems to reduce the suffering as well as the number of animals in research. The EUSAAT Board is pleased that the number of sponsors of the EUSAAT congresses has increased over the years, since without their continuous support we would not be able to maintain the high scientific standard and to keep the congress fee low. Both elements are equally important in order to attract young scientists from all over Europe and beyond. Therefore, the EUSAAT Board and Scientific Committee thank all of the sponsors of EUSAAT 2018 on behalf of the participants. Moreover, we are also pleased that the abstracts of the EUSAAT 2018 conference on the 3Rs are again published in ALTEX Proceedings and we thank the editor and CEO of ALTEX Edition for helping us to pave the way for future coop- eration for the benefit of implementing the 3Rs in Europe. We finally want to thank our colleagues on the EUSAAT Board and the Scientific Committee for their continuous support in planning the EUSAAT 2018 congress. Horst Spielmann Winfried Neuhaus Dagmar Jirova Dominik Rünzler Secretary General President Vice-President Vice-President ALTEX Proceedings 7, 2/18 I ABSTR ACTS LINZ 2018 List of Abstracts ID Title Authors Type 1 The elephant in the room: PREPARE Adrian Smith, R. Eddie Clutton, Elliot Lilley, Oral before you ARRIVE Kristine Hansen and Trond Brattelid 2 A combined in silico-3D lung tumor model to Gudrun Dandekar, Meik Kunz, Sarah S. L. Nietzer, Oral understand network effects of driver mutations for Florentin Baur, Michaela Baier, Claudia Göttlich, targeted therapy predictions Julia Schüler, Ralf C. Bargou, Heike Walles and Thomas Dandekar 3 S. aureus-infection-model at human liver-on-chip Fatina Siwczak, Alexander Mosig and Poster Lorena Tuchscherr de Hauschopp 4 Modelling ischemic stroke injury at the blood-brain Abraham Alahmad and Shyanne Page Oral barrier in vitro using induced pluripotent stem cells 5 Measuring Culture of Care – a practical example Thomas Bertelsen Oral 6 Development of an in-vitro testing battery to Elisabeth Mertl, Christian Kirchnawy, Daniela Poster
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