Norm Impact Assessment Toolkit: from Microorganisms To

NORM IMPACT ASSESSMENT CONTACT E-MAILS: TOOLKIT: FROM [email protected] MICROORGANISMS This project has received funding from the Euratom [email protected] research and training programme 2019-2020 under TO HUMAN CELLS grant agreement No 900009 NORM IMPACT ASSESSMENT TOOLKIT: FROM MICROORGANISMS TO

HUMAN CELLS 30th August to 10th September, 2021 Aveiro and Porto, Portugal

3 MODULES LECTURERS Virginie Chapon (CEA) Microbiota impact assessment Catherine Berthomieu (CEA) Toxicological (cytotoxicity, Ângela Cunha (UA) genotoxicity and omics) assessment Sónia Mendo (UA) Mónica Amorim (UA) Ecotoxicological assessment and Ruth Pereira (UP) contributes for risk assessment Hugo Osório (i3S) Toshihiro Horiguchi (NIES) Tânia Caetano (UA) Cátia Fidalgo (UA) GENERAL INFO Jörg Hackermüller (UFZ) Hands-on course focused on acquiring Magdalini Sachana (OECD) theoretical and practical knowledge on APPLICATION Maximum number of participants: 15 laboratory techniques Joana Pereira (UA) and standardized assays and perceive No fee Kees Van Gestel (VU) their application in NORM contaminated Requirements: Graduation in Biological Jörg Römbke (ECT) scenarios. The advantages of the Sciences, Environmental Sciences or Reinhard Dallinger (UIBK) Health and English language proficiency application of Omics technologies and Joana Lourenço (UA) of the integration genotoxicity and Aplication form at: cytotoxicity endpoints in environmental https://forms.gle/6eCAGJetAZ3hjFUf6 Note: If presencial attendance is restricted risk assessment schemes will be Application deadline: 15th July 2021 due to the COVID19 pandemic, the course addressed. ECTS will be applied to the course will be lectured online. International Advanced course: NORM IMPACT ASSESSMENT TOOLKIT: FROM MICROORGANISMS TO HUMAN CELLS

PROGRAM

This project has received funding from the Euratom research and training programme 2019-2020 under grant agreement No 900009 Advanced course:

NORM IMPACT ASSESSMENT TOOLKIT: FROM MICROORGANISMS TO HUMAN CELLS

PROGRAM

Monday Tuesday Wednesday Thursday (30th Aug) (31st Aug) (1st Sept) (2nd Sept)

Morning

Catherine Berthomieu (CEA) - Ângela Cunha (UA) - Detecting functional Determinants of the interaction of NORM Joana Lourenço (UA) - Toolbox and biomarkers Participants reception, welcoming and changes in bacterial communities - activity species with proteins : Consequences for for genotoxicity detection: application to NORM presentation measurements and physiological profiling their speciation in cells or for the contaminated sites development of strategies of biodetection or bioremediation. Toshihiro Horiguchi (NIES) - Changes in Sónia Mendo (UA) - Extreme environments and invertebrates (intertidal zones) and megabenthos Virginie Chapon (CEA) - Impact of radionuclides Tutorship (data analysis, presentation their microbial relics with immense (coastal waters) off Fukushima at population- on soil bacterial diversity preparation) biotechnological potential and community levels after the 2011 Great East Japan Earthquake and Tsunami and the nuclear disaster Tânia Caetano (UA) - Web tools to predict the Mónica Amorim (UA) - Toxicogenomics: Virginie Chapon (CEA) - Mechanisms of biotechnological potential and the resistance Afternoon from establishing tools to the knowledge bacteria-uranium interaction profile of prokaryotic genomes and towards a systems toxicology.

Afternoon Cátia Fidalgo (UA) - Analysis of contamination Afternoon impact on soil microbiota diversity and function

Joana Pereira (UA) - Epigenetics: from the Afternoon basics to the role in ecotoxicology and environmental risk assessment Free afternoon Practical class (soil eDNA extraction, assessment of DNA integrity and concentration, 16S PCR) Practical class on microbiome data analysis Practical class on comet assay Advanced course:

NORM IMPACT ASSESSMENT TOOLKIT: FROM MICROORGANISMS TO HUMAN CELLS

PROGRAM

Friday Monday Tuesday Wednesday (3rd Sept) (6th Sept) (7th Sept) (8th Sept)

Morning

Jörg Hackermüller (UFZ) - Multi-omics Welcoming of the participantes to the University data integration in toxicology of Porto and presentation

Magdalini Sachana (OECD) - OECD AOP Kees Van Gestel (VU) - Ecotoxicology: Practical class (Comet assay slides visalization, programme and regulatory applications: the Working group Presentations present achievements and future Cytotoxicity assessment) experience of the chemical sector challenges

Hugo Osório (i3S) - Mass-Spectrometry based Proteomics in Radiation and Cancer Practical Class (Avoidance Assay) (Part 1)

Hugo Osório (i3S) - Mass-Spectrometry Afternoon based Proteomics in Radiation and Cancer Afternoon Afternoon (Part 2)

Afternoon

Practical Class (Avoidance assay + Biomarkers) Free afternoon Data analysis (trip to Porto) Practical class on Proteomics Data Analysis

Ruth Pereira (GreenUPorto) - Predictive versus retrospective Risk assessment Advanced course:

NORM IMPACT ASSESSMENT TOOLKIT: FROM MICROORGANISMS TO HUMAN CELLS

PROGRAM

Thursday Friday (9th Sept) (10th Sept)

Morning

Jörg Römbke (ECT) - Standardization of ecotoxicological tests: the work of ISO Reinhard Dallinger (UIBK) - Title to be announced Technical Committee 190 “Soil” as an example

Practical Class (Biomarkers) Practical Class (Avoidance Assay ﬁnalization and calculations)

Afternoon Afternoon

Theoretical/Practical Class (Risk Limits estimation)

Working Group Presentations

Theoretical/Practical Class Biomarkers calculations

Closing session Lecturers

Virginie Chapon Cátia Fidalgo

CEA - BIAM / LIPM Department of Biology & CESAM Biosciences and Biotechnology Institute Aix-Marseille BIAM University of Aveiro UMR 7265 CNRS, CEA, Aix-Marseille Univ. Campus Universitário de Santiago CEA-Cadarache, 13108 Saint Paul-lez-Durance 3810-193 Aveiro France Portugal

Ângela Cunha Catherine Berthomieu

Department of Biology & CESAM CEA - BIAM / LIPM University of Aveiro Biosciences and Biotechnology Institute Aix-Marseille BIAM Campus Universitário de Santiago UMR 7265 CNRS, CEA, Aix-Marseille Univ. 3810-193 Aveiro CEA-Cadarache, 13108 Saint Paul-lez-Durance Portugal France

Sónia Mendo Joana Lourenço

Department of Biology & CESAM Department of Biology & CESAM University of Aveiro University of Aveiro Campus Universitário de Santiago Campus Universitário de Santiago 3810-193 Aveiro 3810-193 Aveiro Portugal Portugal

Tânia Caetano Mónica Amorim

Ruth Pereira Jörg Hackermüller Reinhard Dallinger

Department of Biology & GreenUPorto Helmholtz Centre for Dpt. of Zoology, Faculty of Sciences Environmental Research University of Innsbruck University of Porto Permoserstr. 15 Technikerstraße 25, Rua do Campo Alegre 04318 Leipzig 6020 Innsbruck, Porto Germany Austria Portugal

Toshihiro Horiguchi Kees Van Gestel

Ecosystem Impact Research Section Department of Ecological Science Health and Environmental Risk Division Vrije Universiteit Amsterdam National Institute for Environmental Studies Noord-Holland, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506 Nederlands Japan

Hugo Osório Joana Pereira

Proteomics Scientiﬁc Platform Department of Biology & CESAM i3S - Instituto de Investigação e Inovação University of Aveiro em Saúde Campus Universitário de Santiago Ipatimup - Institute of Molecular 3810-193 Aveiro Pathology and Immunology Portugal Faculty of Medicine, University of Porto Portugal

Jörg Römbke Magdalini Sachana

ECT Oekotoxikologie GmbH Organisation for Economic Co- Böttgerstraße 2 – 14 Operation and Development 65439 Flörsheim am Main (OECD), Germany 75775 Paris Cedex 16 France