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Physical Optics

Lecture 7: 2017-05-17 Herbert Gross

www.iap.uni-jena.de

2 Physical Optics: Content

No Date Subject Ref Detailed Content Complex fields, wave equation, k-vectors, interference, light propagation, 1 05.04. Wave optics G Slit, grating, integral, diffraction in optical systems, point spread 2 12.04. Diffraction B function, aberrations Plane wave expansion, resolution, image formation, transfer function, 3 19.04. Fourier optics B phase imaging Quality criteria and Rayleigh and Marechal criteria, Strehl ratio, coherence effects, two-point 4 26.04. B resolution resolution, criteria, contrast, axial resolution, CTF Introduction, Jones formalism, Fresnel formulas, birefringence, 5 03.05. Polarization G components Energy, momentum, time-energy uncertainty, photon statistics, 6 10.05. Photon optics D fluorescence, Jablonski diagram, lifetime, quantum yield, FRET Temporal and spatial coherence, Young setup, propagation of coherence, 7 17.05. Coherence G speckle, OCT-principle Atomic transitions, principle, resonators, modes, types, Q-switch, 8 24.05. Laser B pulses, power 9 31.05. Gaussian beams D Basic description, propagation through optical systems, aberrations Laguerre-Gaussian beams, phase singularities, Bessel beams, Airy 10 07.06. Generalized beams D beams, applications in superresolution microscopy Apodization, superresolution, extended depth of focus, particle trapping, 11 14.06. PSF engineering G confocal PSF Basics of nonlinear optics, optical susceptibility, 2nd and 3rd order effects, 12 21.06. Nonlinear optics D CARS microscopy, 2 photon imaging Introduction, surface scattering in systems, volume scattering models, 13 28.06. Scattering D calculation schemes, tissue models, Mie Scattering 14 05.07. Miscellaneous G Coatings, diffractive optics, fibers

D = Dienerowitz B = Böhme G = Gross 3 Contents

Coherence

. Introduction . Young's experiment . Spatial coherence . Temporal coherence . Partial coherent imaging . Speckle . OCT principle

4 Coherence in Phase Space

coherent : every point partial coherent :every point has incoherent : every point radiates in one direction an individuell angle characteristic radiates in all directions line in phase space finite area in the phase space filled phase space

u u u

x x x

5 Coherence in Optics

. Statistical effect in wave optic: start phase of radiating light sources are only partially coupled . Partial coherence: no rigid coupling of the phase by superposition of waves . Constructive interference perturbed, contrast reduced . Mathematical description: Averagedcorrelation between the field E at different locations and times: Coherence function G . Reduction of coherence: 1. Separation of wave trains with finite spectral bandwidth Dl 2. Optical path differences for extended source areas 3. Time averaging by moved components . Limiting cases: 1. Coherence: rigid phase coupling, quasi monochromatic, wave trains of infinite length 2. Incoherence: no correlation, light source with independent radiating point like molecules

6 Coherence Function

. Coherence function: Correlation x of statistical fields (complex)    *  (r ,r , )  E(r ,t  )E (r ,t) x1 1 2 1 2 t E(x ) Dx 1 z for identical locations : x    2 E(x ) r1 r2 r 2 intensity    (r,r)  I(r)

.  normalized: degree of coherence     (r1,r2 , )  12(  )  (r1,r2 , )    I(r1)I(r2 ) . In interferometric setup, the amount of  describes the visibility V . Distinction: 1. spatial coherence, path length differences and transverse distance of points 2. time-related coherence due to spectral bandwidth and finite length of wave trains

7 Axial Coherence Length of Lightsources

Light source lc

Incandescent lamp 2.5 m

Hg-high pressure lamp, line 546 nm 20 m

Hg-low pressure lamp, line 546 nm 6 cm

Kr-isotope lamp, line at 606 nm 70 cm

HeNe - laser with L = 1 m - resonator 20 cm

HeNe - laser, longitudinal monomode stabilized 5 m

8 Double Slit Experiment of Young

. First realization: change of slit distance D

. Second realization: change of coherence parameter s of the

source light source

screen with slits

z1 detector V 1

D

x z2

Dx

0 D

9 Double Slit Experiment of Young

. Young interference experiment:

. Ideal case: point source with distance z1, ideal small pinholes with distance D

. Interference on a screen in the distance z , intensity 2 Dx 2 I(x)  4I0 cos l z2 lz . Width of fringes Dx  2 D x

detector

source D  region of interference

screen with z2 pinholes

z2

10 Double Slit Experiment of Young

. Partial coherent illumination of a double pinhole/double slit . Variation of the size of the source by coherence parameter s . Decreasing contrast with growing s

. Example: pinhole diameter Dph = Dairy / distance of pinholes D = 4Dairy

s = 0 s = 0.15 s = 0.25 s = 0.30 s = 0.35 s = 0.40

11 Coherence Measurement with Young Experiment

. Typical result of a double-slit experiment according to Young for an Excimer laser to characterize the coherence . Decay of the contrast with slit distance: direct determination of the transverse coherence

length Lc

12 Spatial Coherence

observation . Area of coherence / transverse coherence length: area

Non-vanishing correlation at two points with distance Lc: Lc   Correlation of phase due to common area on source  ( r , r ) P1 1 2 domain of coherence . Radiation out of a coherence cell of P2 r1 extension Lc guarantees finite contrast r2 O

. The lateral coherence length starting receiving plane plane changes during propagation: spatial coherence grows with increasing propagation distance 1

2

common area

13 Spatial Coherence

. Incoherent source with diameter D = 2a . Receiver plane indistance z . Cone of observation

 l / a

. Source is coherent in the distance z  a 2 / l

. Transverse length of coherence (zeros of -function) l z l L   c a 

14 Near- and Farfield of Excimer

. Parameter of real Excimer lasers

Near field (spatial) Far field (angle)

157 nm - laser

193 nm - laser

248 nm - laser

15 Van Cittert - Zernike - Theorem

2 . Propagation of coherence function: i 2 2 i       1  (r1 r2 )   r '(r1 r2 )     (r ,r , z)   e lz I(r',0)e lz dr' in special case r 'r 'r ' 1 2    1 2  lz 

. Van Cittert-Zernike theorem: Coherence function of an incoherent source is the Fourier transform of the intensity profile . Example: circular light source wirh radius a

V  2 ar 2J   1 l z  (r)   a2  1 2 ar l z vanishing contrast

Vanishing contrast at radius l r  0.61 z  a

r

16 Gauß-Schell Beam: Definition

. Partial coherent beams: 2 1  r r   1 2  1. intensity profile gaussian    2  Lc  2. Coherence function gaussian (r1 r2 , z  0)  e

. Extension of gaussian beams with similar description

. Additional parameter: lateral coherence length Lc 1   2  2 . Normalized degree of coherence  w    1  o     L     c   2 2 1  wo  . Beam quality depends on coherence M   1     L   c 

. Approximate model do characterize multimode beams

17 Gauss-Schell Beams

. Due to the additional parameter: w / wo Waist radius and divergence angle are 4 independent 3  0.25

1. Fixed divergence: 2 waist radius decreases with  0.50 1 growing coherence  1.0

0 z / zo -4 -3 -2 -1 0 1 2 3 4

w / wo

4  0.25  0.50

3 2. Fixed waist radius: divergence angle decreases with 2  1.0 growing coherence 1

0 z / zo -4 -3 -2 -1 0 1 2 3 4

18 Temporal Coherence

. Damping of light emission: wave train of finite length . Starting times of wave trains: statistical

U(t)

t

c

duration of a single train

19 Temporal Coherence

. Radiation of a single atom: Finite time Dt, wave train of finite length, 2 i t No periodic function, representation as Fourier integral E(t)   A()e d with spectral amplitude A()

sin  Dt . Example rectangular spectral distribution A( )    Dt

. Finite time of duration: spectral broadening D, D1/Dt schematic drawing of spectral width I()

20 Axial Coherence Length

. Two plane waves with equal initial phase and differing l1, l2 . Idential phase after axial (longitudinal) coherence length c l  c  c c D

l1 l 2 time t

starting phase difference in phase phase 180°

21 Time-Related Coherence Function

. Time-related coherence function: 1 T ( )  lim E*(t) E(t   )dt  E*(t) E(t   ) Auto correlation of the complex field E T  T 2T T at a fixed spatial coordinate . For purely statistical phase behaviour:  = 0

. Vanishing time interval: intensity (0) E*(t) E(t)  I T

. Normalized expression * ( ) E (t) E(t   )  ( )   2 (0) E(t) | (  ) | . Usually:  decreases with growing  symmetrically

Width of the distribution: c

c

22 Time-Related Coherence Function

. Intensity of a multispectral field  Integration of the power spectral density S() I   S( )d 0

. The temporal coherence function and the power  2i spectral density are Fourier-inverse: S( )   ( )e d Theorem of Wiener-Chintchin 

1 . The corresponding widths in time and spectrum are  c  related by an uncertainty relation D

 . The Parceval theorem defines the coherence time    ( ) 2d c  as average of the normalized coherence function 

. The axial coherence length is the space equaivalent of l  c the coherence time c c

23 Michelson-Interferometer

. Michelson interferometer: interference of finite size wave trains . Contrast of interference pattern allows to measure the axial coherence length/time

second mirror

signal beam relative moving moving z wave trains z with finite length

I(z) reference overlap beam

lc first mirror beam from splitter receiver source

24 Interference Contrast

. Superposition of plane wave with initial phase  I  Im  2 In  Im cosn m  Intensity: m nm

     . Radiation field with coherence function : I(r) I1(r)I2 (r)2r1,r2,0 . Reduced contrast for partial coherence   Imax  Imin (r1,r2 , ) I K     filtered Imax  Imin I(r1)I(r2 ) signal measured signal . Measurement of coherence in Michelson interferometer: phase difference due to path length difference in the two arms (Fourier spectroscopy)

4 Dl  z z D  2Dk  z  2 measured l position axial length of coherence

25 Axial Coherence

. Contrast of a 193 nm excimer laser for axial shear . Red line: Fourier transform of spectrum

contrast

1

0,9 measured 0,8 FFT-Data

0,7

0,6

0,5

0,4

0,3

0,2

0,1 z-shift in mm 0 -0,8 -0,6 -0,4 -0,2 0 0,2 0,4 0,6 0,8

26 Coherence Parameter

Heuristic explanation coherent small stop of condenser object objective lens of the coherence illumination condenser parameter in a system: Psf of observation extended inside psf of 1. coherent: source illumination Psf of illumination large in relation to the observation

Large s

incoherent illumination large stop of condenser 2. incoherent: Psf of observation Psf of illumination extended contains several small in comparison source illumination psfs to the observation

Small s

sinu s  ill sinuobs

27 Coherence Parameter

. Finite size of source : aperture cone with angle uill . Observation system: aperture angle uobs

. Definition of coherence parameter s: sinu Ratio of numerical apertures s  ill sinuobs . Limiting cases: coherent s = 0

uill << uobs

incoherent source object lens image x , y s = 1 o o xi , yi

uill >> uobs

uill uobs

illumination observation

28 Partial Coherence

Simulation of partial coherent illumination: . Finite size of light source . Corresponding finite size of illuminated area in aperture plane . Every point in this area is considered to emit independent (incoherent) . Off-axis point in aperture plane generates an inclined plane wave in the object . Angular spectrum illumination of the object

describes partial coherence Ls 2s  2arcsin . Estimated sampling of illumination points: 2 fc

0.61l aperture condenser object Dys  plane plane

n sin fc angular source spectrum of  2 extension s object illumination Ls

Dys size of coherence cell

29 Partial Coherent Imaging

. Image intensity: 1. Correlation of two points in the object 2. Integration over all points in the incoherent light source

I x  I x h x , x , x h* x , x , x h x , x h* x , x O x O* x dx dx dx i  i    s  s  obs i o1 s  obs i o2 s  ill  o1 s  ill  o2 s   o1  o2  o1 o2 s

light object pupil image source plane

ys yo y yi p

xo xs xp xi z

P(xp) o(xo1,xo2) 'o(xo1,xo2)

hill(xL,x1) hobs(x1,x') Ii(xi) Is(xs) O(x1)

. Simplification: thin object, transmission does not change for moderate inclination angles

I x   x , x h x , x h* x , x O x O* x dx dx i  i   o  o1 o2  obs i o1 obs i o2   o1  o2  o1 o2

Transmission Cross Correlation Function (TCC)

. Integration overlap of pupils a) partial coherent

pupil light . Typical chnage of x source transfer capability b) incoherent

HMTF()

1 coherent x s = 0

incoherent partial s = 1 0.5 coherent contrast 0  s < 1 increased contrast decreased

threshold 0  o loss of 2o resolution

Example: Partial Coherent Edge Image

solid line : exact , dashed : approximation s = 0.2 = 0.5 = 0.8 . Image formation of a phase edge s s 1 1 1 under partial coherent illumination  = 0° .  : phase step 0.5 0.5 0.5 0 0 0 s : degree of coherence -2 -1 0 1 2 -2 -1 0 1 2 -2 -1 0 1 2 1 1 1  = 30° 0.5 0.5 0.5

0 0 0 -2 -1 0 1 2 -2 -1 0 1 2 -2 -1 0 1 2

1 1 1  = 60° 0.5 0.5 0.5

0 0 0 -2 -1 0 1 2 -2 -1 0 1 2 -2 -1 0 1 2

1 1 1  = 90° 0.5 0.5 0.5

0 0 0 -2 -1 0 1 2 -2 -1 0 1 2 -2 -1 0 1 2

1 1 1  = 180° 0.5 0.5 0.5

0 0 0 -2 -1 0 1 2 -2 -1 0 1 2 -2 -1 0 1 2

32 Example: Partial Coherent Imaging of Bar-Pattern

12 m s = 0.08 s = 0.50 s = 1.0 object pupil intensity

object image spectrum 33 Example: Partial Coherent Imaging of Siemens Star

coherent partial coherent incoherent

frequency frequency o = sinu / l o = 2sinu / l 34 Speckle Effect

. Generation of speckles: Coherent light is refracted / reflected at a rough surface . Roughness creates phase differences . Interference of all partial waves: granulation, signature for a local surface patch . Transmission of random media in a volume is also possible (atmosphere, biological) . Higher order effects: patial coherent illumination, polarization

incident laser light

plane of observation

surface with roughness 35 Sum of Random Phasors

. Sum of random phasors due to field superposition: 1. nearly zero result, dominant destructive 2. large result, dominant constructive 3. special case of one large contribution

Ref. J. Goodman

36 Speckle Pattern

. Size of objective speckles: depends on distance of z = 840 mm z = 330 mm observation

. Colored speckles

z = 160 mm z = 110 mm 37 Subjective / Objective Speckle

. Creating of speckle pattern: 1. coherent scattering of laser light: incident laser light objective speckle

2. imaging of coherent straylight: subjective speckle always be visual observation r1 P point of r2 observation surface with lens with focal roughness length f p > l

intensity D d

surface with roughness schreen z z' 38 Objective Speckle Pattern

. Incident coherent light . Rough surface with size D . Observation in distance z l  z D . Speckle pattern with typical size of cells d   Airy D 2

incident coherent laser light intensity

D rough surface

d

screen

z 39 Subjective Speckle Pattern

. Incident coherent light lens with focal . Rough surface with size D length f . Observation in distance z . Speckle size in the image: intensity ds D d PSF, Dairy . Speckle pattern with typical size of cells in the object l surface with roughness ds  (1 m)  (1 m)  Dairy schreen 2NA z z' m: magnification F#= 22 F#= 66 . Example: coarse speckle for small NA

Ref. W. Osten

40 Statistics of Superposed Speckles

w(I) . Incoherent superposition of several speckles 1

0.9

0.8 . Probability has intermediate maximum 0.7 I 0.6 2 4I I 0 0.5 w(I)  2 e I0 0.4 . Zero probability for darkness 0.3 0.2

. Decreasing contrast 0.1

0 I / Io 0 0.5 1 1.5 2 2.5 3 . Example

41 Speckle Statistics for Incoherent Superposition

. Reduction of speckle contrast by incoherent superposition

w(I) . Overlay of large number of individual fully modulated 1 images 0.9

0.8 . Many images necessary to n = 2 get a uniform illumination 0.7 n = 6

0.6 n = 12

. Reduction of variance goes 0.5 n = 20 with 1/ n 0.4 n = 40

0.3 n = 100

0.2

0.1

0 I / I 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 o 42 Speckle Reduction

. Coherent speckles after diffusor plate with different data

starting phase

spectrum

far field 43 Speckle Contrast Changing with Coherence

. Contrast of speckle image for changing coherence . a: amplitude lc: transverse lenght of coherence

a/lcorr = 0 a/lcorr = 0.1 a/lcorr = 0.5

a/lcorr = 1.0 a/lcorr = 2.0 a/lcorr = 4.0 44 Scattering in Turbid Media

. Different strengths of interaction a) ballistic photon

b) snake photons

c) multiple scattered photons

Ref: M. Gu 45 Scattering in Turbid Media

. Change of light properties

a) spectral shift b) spatial broadening

Dw

snake frequency  D x x frequency 

c) temporal broadening d) polarization snake

time t Dt time t

Ref: M. Gu 46 Resolution in OCT

2ln 2 l2 l2 1. Axial resolution limited by spectral bandwidth Dzcoh   0.4413 Low NA High NA  Dl Dl

2Dx 2Dx

2Dzdiff

2Dz coh 2Dz diff

2. Lateral resolution: diffraction limited, improvement by confocal setup 3. Usually low NA

47 Principle of OCT

. Basic method of optical coherence tomography: - short pulse light source creates a coherent broadband wave - white light interferometry allows for interference inside the axial coherence length . Measured signal: - low pass filtering - maximum of envelope gives the relative length difference between test and reference I(z) arm l2 Dl  o filtered signal Dl signal measured . For Gaussian beam 4ln 2 l2 Dl   o  Dl . High frequency oscillation depends on z 4 Dl  z D  2Dk  z  z l2 measured position coherence length

48 Fiber Based OCT Interferometer

I Basic setup source spectrum

surface measuring under test LED arm source fiber coupler

fiber fiber

fiber I signal fiber

detector reference arm z-scan z

49 Optical Coherence Tomography

. Example: sample with two reflecting surfaces . 1. Spatial domain

2. Complete signal

3. Filtered signal high-frequency content removed

Ref: M. Kaschke

50 Optical Coherence Tomography

. Achronyms in literature

Ref: R. Leach

51 Resolution of OCT

. Lateral resolution: Airy profile

4l  f 2l lateral Dx   resolution    sin u Log Dx

. Penetration depth: axial resolution 100 m

ultra sound 2ln 2 l2 Dzres    Dl 10 m confocal microscopy OCT

1 m

Log Dz 100 m 1 mm 1 cm 10 cm depth

52 Example of OCT Imaging

Example:

Fundus of the human eye

53 White Light Interferometry

. Examples

Ref: R. Kowarschik

54 Spectral Domain OCT

. Spectral Domain-OCT: - broad band source - reference mirror fixed in position, no A-scan necessary - signal splitted by spectrometer . The high-frequency content of the signal is analyzed . The frequency is proportional to the depth z, measured is the overlay beat-signal of all scatterers reference mirror

Sample in z fixed

z Broadband source

interferogramm Fourier transform frequency domain spatial domain

spectrometer

 data z processing

55 Fourier Domain OCT

. Fourier Domain-OCT: setup

. Signals: a) intensity spectrum b) spatial intensity distribution

Ref: M. Kaschke

56 Fourier Domain OCT Signal

1.4

1.2 1 scatterer

1

. Signal complexity depends on 0.8 scatter-distribution 0.6 0.4

0.2

0 0.8 0.85 0.9 0.95 1 1.05 1.1 1.15 1.2 1.4

1.2 2 scatterer

1

0.8

0.6

0.4

0.2

0 0.8 0.85 0.9 0.95 1 1.05 1.1 1.15 1.2 1.4 rS(zS) reflectivity reference sample 1.2 3 scatterer

1

0.8

0.6

0.4

0.2 zS zR zS1 zS2 0 0.8 0.85 0.9 0.95 1 1.05 1.1 1.15 1.2

A-scan ID(z) DC term

cross correlation mirror image artifacts auto correlation z 2(z -z ) 2(z -z ) -2(z -z ) -2(z -z ) R S2 R S1 0 R S1 R S2