Core Research Facilities Enabling research excellence “We will invest in major new capabilities for our core research facilities

that will provide our Enabling research excellence researchers with the

infrastructure, tools and Research Core Facilities Director’s welcome �������������������������������������������������������� 3 Our research services ��������������������������������������������������� 4 Access our facilities �������������������������������������������������������� 5 technical support to pursue Research and Prototype Foundry ���������������������������� 6 Analytical ����������������������������������������������������������� 8 Sydney Cytometry �������������������������������������������������������� 10 research excellence.” Sydney Imaging ������������������������������������������������������������ 12 Sydney Informatics Hub �������������������������������������������� 14 Sydney Mass Spectrometry �������������������������������������� 16 Sydney Microscopy & Microanalysis ���������������������� 18 Sydney Manufacturing Hub �������������������������������������20 Our locations ����������������������������������������������������������������22 Contact us ����������������������������������������������������������������������24

The 2016-20 Strategic Plan Director’s welcome Professor Simon Ringer “World-class infrastructure, Director, Core Research Facilities user-centric processes and the . au . edu skills, talents and passions of a Director’s welcome Director’s sydney team dedicated to supporting

At the University of Sydney, our in climate, health, food, water, The facilities are designed for use research is driven by the pursuit energy, communications, transport, by academic teams in the physical, the research ambitions of the of excellence. The University's manufacturing, construction medical, life and engineering sciences unprecedented focus on research and national security. and throughout the humanities and and research infrastructure is social sciences. institution and our partners— transforming our capacity to drive The facilities bring together world- both fundamental and translational class instrumentation, outstanding They serve as a focal point for research for the benefit of people and excellent user-focused collaborations and partnerships and the world. processes. They provide staff and with researchers from these diverse this is the essence of Sydney's students from the University and disciplines tackling frontier research The continuing development of our our partners around the world with questions. core research facilities is an integral an exceptional basis on which to part of this strategy. Our research achieve research excellence. If you or your research team could The University of Sydney University The core research facilities.” facilities are intimately linked to benefit from the facilities at Sydney, our search for solutions to the most I encourage you to make contact to significant contemporary challenges discuss the possibilities.

Page 2 Page Professor Simon Ringer 3 Page Our research services Access your research facilities

Lab support is an important part of any grant Project development Our staff are a constant presence in application. We can assist you with Our specialist technical each of our facilities, and are available this during the grant‑writing process. staff are on hand to help at any time to provide hands-on design your research project from technical assistance. Assisted access to national the ground up, ensuring you get the research facilities most from our facilities. Data analysis and With our established visualisation networks of research partners, we . au Instrument training We have vast capabilities and can assist you in accessing specialist Consultation Activation Induction Supervision . edu Through comprehensive expertise for advising and supporting research facilities throughout Our staff will work with you We will assist you in We will organise a bespoke Our specialist staff will Access your research facilities research your Access sydney one-on-one training, you you in analysing, visualising and Australia. to put together a viable registering your project in training program specific be onsite to provide will acquire the skills to operate the publishing your research data. project design, taking our online Facility Access to your needs, giving you any technical assistance full range of instruments required by Guidance on advanced computing into account factors such System, and will guide you autonomous access to our you require. your research. Contract research and testing Through consultation, training courses as experimental scope, through any regulatory facilities to complete your We have a strong track record or one-on-one support, we can instrument requirements, approvals necessary. project. Regulatory compliance of providing high-quality help you with all your HPC, VRD and and budget. You will be guided through research and testing services for research data needs. the necessary safety, clients, and can accommodate small ethics and building access protocols and large scale projects. tailored to your project’s scope. User Access Scheme Facts and figures Assistance with grant preparation The Core Research Facilities User Access Scheme has been developed Up-to-date facility access information to invest in and drive research excellence across the University by 8 providing small grants to support user access to the facilities. openly available specialist facilities

These grants are aimed at supporting important research that would significantly benefit from use of the core research facilities, as well

The University of Sydney University The Major partner research organisations: 80+ as supporting preliminary, supplementary or additional research onsite experts providing related to funded research projects, to best leverage the outcomes guidance and research services from awarded grants.

Interested in applying for a grant? Find out how: 400+ − sydney.edu.au/research/facilities/user-access-scheme instruments available for users Page 4 Page 5 Page The Research and Prototype Foundry, −− ASML PAS 5500/100 −− Focused ion beam based at the Sydney Nanoscience Hub, i-Line stepper scanning electron microscopy offers instruments for the fabrication of −− SVG 88 track system −− PM 5 probe station devices and structures with features on −− Oxford ICP reactive ion etcher −− Leica stereo zoom the micro and nanoscale, with specialised −− Atomic layer deposition microscopy processes allowing users to prototype new Research −− DWL 66+ laser −− Olympus stereo optical chips, electronic devices and new mask writer zoom microscopy quantum science and technology devices. −− Elionix electron −− DekTak stylus . au beam lithography profilometer . edu Our goal is to provide ready access to −− Atomic force microscopy −− ISO Class 5 cleanroom. Research and Prototype Foundry and Prototype Research sydney and state-of-the-art tools for micro and −− Brewer spin coater Research in action  nano fabrication (lithography, etching, −− Brewer developer This image shows an deposition and metrology) backed up by a −− MLA 100 Maskless optical ring resonator team of expert process engineers. aligner device for high frequency −− Spin dryer signal processing. This was Prototype More information produced by a photonics −− Wet benches − sydney.edu.au/research-and-prototype-foundry

Foundry Our capabilities

researcher, using several different capabilities within the Research and Prototype Foundry cleanroom. The Foundry is capable of

The University of Sydney University The printing details less than 10nm wide using one of the most powerful Professor Simon Fleming Dr Nadia Court electron-beam lithography instruments in the Southern Hemisphere. Academic Director Technical Director +61 2 9114 0851 +61 2 8627 8671 [email protected] [email protected] Page 6 Page 7 Page Sydney Analytical is the Our capabilities University’s core research −− Vibrational spectroscopy: −− Controlled sample facility dedicated to materials, Raman, infrared and near- environments, including chemical and biological analysis. infrared spectrometers, temperature, pressure Our instruments are operated offering 2-D and 3-D mapping and battery cells. in four principle clusters: at resolutions down to −− Support for early-stage drug nanometres with AFM/ vibrational spectroscopy, x-ray discovery: protein production spectrometer techniques. and characterisation, protein techniques, magnetic resonance, −− X-ray diffraction (single- X-ray crystallography, . au and drug discovery. crystal and powder X-ray) fragment-based drug . edu and X-ray scattering. discovery, and cyclic Sydney Analytical Sydney sydney Sydney We provide instruments and −− X-ray spectroscopy: XPS peptide screening. services to researchers from across and UPS mapping (30 μm) −− Magnetic resonance: NMR and depth profiling, XRF spectrometers from 300 the spectrum of life and physical −− Portable spectrometers: to 800 MHz and EPR. sciences, engineering, and Raman, infrared and XRF Analytical beyond. −− Specialist capability in the characterisation of cultural More information heritage materials. − sydney.edu.au/sydney-analytical

Research in action  Portable vibrational spectroscopic techniques are The University of Sydney University The being used for the identification of faded pigments Professor Peter Lay Professor Margaret Sunde Sydney Analytical enquiries on a 2500-year‑old coffin believed to belong to the Academic Director Deputy Director +61 2 8627 6903 woman Mer‑Neith-it-es. The coffin was donated to +61 2 9351 4269 +61 2 9351 6955 [email protected] the University in 1860 by Sir Charles Nicholson. [email protected] [email protected] Image courtesy of the Nicholson Museum (NMR.29.1-2) Page 8 Page 9 Page Jointly operated by the University of −− Cell analysis by fluorescence-based flow cytometry. Sydney and the , The facility provides a range of instruments from basic 3-laser, 10-parameter cytometers to Sydney Cytometry provides access to a unique custom-built instrument with 10-lasers cytometry and cell-sorting techniques and 30-parameters and one of the first next- for researchers both internal and generation spectral flow cytometers external to the University. We are −− Cell and tissue analysis by imaging flow committed to the development and cytometry and high-content bio imaging transfer of expertise in quantitative −− Cell and tissue analysis by mass cytometry, including the Southern Hemisphere’s first imaging mass . au cell science to answer questions in cytometer that enables the simultaneous localisation of . edu cell biology and biomedical research, up to 45 different molecules at microscopic resolution Sydney Cytometry Sydney sydney Sydney applied clinical research and trials, −− Cell sorting via automated magnetic separation and the diagnosis of cancer and other or high-throughput droplet-based cell sorting of health disorders. multiple populations at speeds up to 30,000 cells per second. In addition, the Centenary Institute has a cell sorter within a PC3-facility, enabling More information Cytometry the sorting of highly biohazardous samples. − sydneycytometry.org.au Research in action  Our capabilities Our imaging mass cytometer is helping us understand

how the immune system responds to viral infection by simultaneously measuring 40 cellular or viral protein targets on tissue sections of the central nervous

The University of Sydney University The system. In this example, we are tracking the spread of Dr Adrian Smith Avrill Aspland virus throughout the central nervous system as well Technical Director Operations Coordinator as investigating the response of immune cells, such +61 2 8627 1828 +61 2 9114 2199 as the brain‑resident microglia. [email protected] [email protected] Page 10 Page 11 Page Offering world-class imaging capabilities −− MRI (preclinical): 3T, 7T and technical expertise, Sydney Imaging −− MRI (clinical): 3T (I-MED Radiology) is a unique facility for research and −− PET/MRI (preclinical): 3T education in the field of biomedical −− Body composition EchoMRI science. We operate a diverse range −− Optical bioluminescence and fluorescence imaging of preclinical and clinical imaging −− High-frequency ultrasound modalities, and a world-class hybrid −− Photoacoustic imaging theatre providing the capability for −− 3D microCT

. au translational research along with training −− 2D x-ray with DXA camera . edu and development of specialised and −− Hybrid operating theatre Sydney Imaging Sydney sydney Sydney complex surgical skills. −− Artis Pheno C-arm: x-ray, fluoroscopy and CT −− Specialist clinical ultrasound (including More information TOE echocardiography) −− Kuka LBR Med Surgical Robot − sydney.edu.au/sydney-imaging −− PET/CT and PET/SPECT/CT (preclinical) – (ANSTO) Our capabilities Imaging −− Small Animal Radiation Research Platform – (Kolling).

Research in action  Non-invasive real-time in utero high-frequency ultrasound imaging of a rat embryo at gestational day E12 for echocardiographic assessment with colour The University of Sydney University The doppler. This modality allows researchers to perform Professor Fernando Calamante Elizabeth Blanchard longitudinal cardiac assessment from embryonic Academic Director Operations Manager gestation to adulthood including heart muscle thickness, +61 2 9114 4293 +61 2 9114 4235 haemodynamic assessment as well as oxygenation/ [email protected] [email protected] haemoglobin saturation of different regions of the heart when combined with photoacoustic imaging. Page 12 Page 13 Page The Sydney Informatics Hub Our capabilities works with and supports the −− Expert data consultancy and training research community by providing −− Software development and application support comprehensive research data −− Digital research tool support, consultancy and services, leveraging staff expertise training (Redcap, eNotebook, Research Data and capabilities in high performance Store (RDS), Dropbox, GitHub, Cloudstor) computing, data analytics, −− Data analytics/science, visualisation, image bioinformatics, and research data processing and publishing support and training

. au Sydney −− Bioinformatics support and software (CLC Genomics management, statistics and strategy. . edu Workbench and Server, Ingenuity Pathways Analysis) Sydney Informatics Hub Informatics Sydney sydney More information −− Statistical consulting and support (e.g. experimental design, optimal statistical analyses, training) − sydney.edu.au/informatics-hub −− HPC (Artemis, Pawsey, NCI), Argus Virtual Research Informatics Desktop and cloud computing support −− Research data planning, collection, storage, sharing, and security −− Advise on data and computing infrastructure policy and strategy. Hub Research in action 

Species

By processing high-throughput sequencing data on the 0.5 Artemis High Performance Computing cluster, researchers were able to find 214 RNA viruses in frogs, fish and reptiles that have never been seen before. It shows RNA viruses The University of Sydney University The are many millions of years old and have existed with and Professor Thomas Bishop Sydney Informatics Hub enquiries evolved alongside the first vertebrates. These findings Academic Director +61 2 8627 6553 contribute to our understanding of disease-causing +61 2 8627 1056 [email protected] viruses. The research was published in Nature, 2018. [email protected] Page 14 Page 15 Page Sydney Mass Spectrometry supports 480, Eclipse) and 6600 Triple ToFs dedicated to research with global impact through a discovery proteomics, including phosphoproteomics portfolio of 20 mass spectrometers for −− Glycomics using an Orbitrap Fusion with ETD proteomics, glycomics, metabolomics, −− Targeted metabolomics and lipidomics on two QTRAPs or two TSQ Altis QQQs lipidomics, and mass spectrometry −− Multiple discovery metabolomics/lipidomics platforms imaging applications. including Q Exactive HFX, Synapt G2 Si, Fusion −− Mass spectrometry imaging via MALDI We partner with research groups both (UltrafleXtreme TOF-TOF) or DESI (Synapt G2 Si)

. au Sydney internal and external to the University −− A Lumos (with ETD, UVPD, 1M) for cross-linking . edu to answer questions in biomedical interactomics analysis and other applications Sydney Mass SpectrometrySydney sydney research, plant biology, applied −− Bioinformatics suite equipped with over animal and human clinical research, 6 high-performance data-processing workstations and a wide range of analysis packages Mass neuroscience and many other areas.

More information

− sydney.edu.au/mass-spectrometry

Spectrometry Our capabilities −− We have several orbitrap platforms (HFX, Research in action  The concentration of cocaine in the brains of honey bees was directly measured using a QTRAP mass spectrometer. Why? Honey bees are a well-established model for behavioural studies, and the research group were able

The University of Sydney University The to show that cocaine may act by directly altering DNA Professor Stuart Cordwell Dr Ben Crossett methylation. Their observations could shed light on Academic Director Associate Director why cocaine has such an enduring impact on behaviour. +61 2 9351 6050 +61 2 9351 6010 [email protected] [email protected] Page 16 Page 17 Page One of the largest and most comprehensive Our capabilities facilities of its kind in the world, Sydney −− Atom probe tomography Microscopy & Microanalysis enables insights into −− Transmission electron microscopy how materials work – at the scale of living cells, −− Scanning probe microscopy tissue, molecules, crystals and even individual −− Scanning electron microscopy atoms. −− X-ray microscopy −− Light and laser microscopy The facility offers access to specialist staff, −− Super-resolution microscopy . au Sydney instruments and training to enable research

. edu −− Correlative light and electron microscopy into physical and biological structures across a Sydney Microscopy & Microanalysis Microscopy Sydney sydney −− Cyro electron microscopy variety of lengths and time scales. This expertise −− Expert specimen preparation extends from specimen preparation to data −− Image visualisation. Microscopy & capture and data analysis, and can be used across multiple disciplines.

More information

− sydney.edu.au/research/facilities/ Microanalysis sydney-microscopy-and-microanalysis

Research in action  Our facilities support research better than 0.6 Ångström, recorded into both inorganic and on the new double-corrected FEI The University of Sydney University The biological structures such as the Themis-Z microscope operated with Associate Professor Filip Braet Eleanor Kable examples pictured on the right. monochromator excited at 300 kV. Deputy Director Facility Manager +61 2 9351 7619 +61 2 9351 7566 Top: Aberration-corrected scanning Below: Confocal micrograph [email protected] [email protected] transmission electron micrograph of colorectal cancer cells at the of GaN imaged along the [211] zone micrometre scale.

Page 18 Page axis, demonstrating spatial resolution 19 Page Additive manufacturing of advanced metals, Our capabilities ceramics and polymers, materials processing −− 3D printing: a digitally −− Heat treatment: facilities, materials testing platforms and world- enhanced alternative to −− Programable inert/active class expertise are the essence of the Sydney traditional ‘subtractive’ atmosphere furnaces, Oil Manufacturing Hub. Geared to support R&D on manufacturing and Salt Baths, Ovens the design and development of new materials, −− Printing materials, −− Metal melting/casting including: manufacturing processes and integration, −− Design: −− Metals (Stainless this unique, research-focused manufacturing −− ANSYS software package Steel, Al, Ni, Ti, based . au Sydney facility enables concept-to-production and other alloys) −− Computer Aided . edu Design (CAD) systems capabilities in the areas of health, defence, −− Ceramics (Al2O3, Sydney ManufacturingSydney Hub sydney transport, oil & gas, mining and construction. SiN, ZrO2 etc) −− Finishing, Metrology and Testing: Facilitating researcher‑industry interfaces is a −− Polymers (PLA, ABS, specialty. PETG, Nylon) −− Precision Electro- Manufacturing discharge Machine −− Printing Technologies: cutting More information −− Vat Polymerisation −− Surface profiling - LiDAR − sydney.edu.au/sydney-manufacturing-hub −− Electron Beam Melting −− Mechanical testing −− Direct Metal - Nanoindentor Hub Laser Melting −− State-of-the-art −− Fuse Deposition mechanical testing Research in action Modeling The Cerafab 7500 3D ceramic printer at Sydney Manufacturing Hub was used to manufacture a bionic eye-lid implant for the BLINC project which aims to restore blinking in patients with facial paralysis. The inability to blink presents a unique problem, as blinking is required The University of Sydney University The to lubricate the eye, without which the eye can dry Associate Professor Gwenaelle Proust Renee Barber out and be damaged, ultimately leading to blindness. Deputy Director Operations Manager Ceramic printing of Aluminium Oxide was selected for its +61 2 9036 5498 +61 2 8627 7226 biocompatibility and non-magnetic properties. Ceramics [email protected] [email protected] are normally difficult to work with in R&D, however leveraging 3D printing allows for a much higher degree of flexibility and agility in design. Page 20 Page 21 Page Forest Lodge

Locations of our facilities Kolling Institute Glebe Our locations (Royal North Shore Hospital, St Leonards) Research and Prototype Foundry Sydney Nanoscience Hub Parramatta Road Sydney Analytical Madsen Building Molecular Bioscience Building School of Chemistry Sydney Institute of Agriculture Sydney Nanoscience Hub . au Chippendale . edu Sydney Cytometry Our locations sydney Centenary Institute Charles Perkins Centre

School of Chemistry Sydney Imaging Centenary Institute City Road Sydney Nanoscience Hub Charles Perkins Centre Kolling Institute Madsen Building Brain and Mind Centre School of Aerospace, (94-100 Mallett Street) + Mechanical and Sydney Informatics Hub Royal Prince Camperdown Campus Mechatronic Engineering Merewhether Building Alfred Hospital Sydney Mass Spectrometry Camperdown Merewhether Molecular Charles Perkins Centre

Missenden Road Building Bioscience Darlington Kolling Institute Building Sydney Microscopy and Microanalysis Brain and Mind Centre The University of Sydney University The Carillon Avenue Darlington Charles Perkins Centre Madsen Building Newtown Campus Sydney Nanoscience Hub Sydney Manufacturing Hub School of Aerospace, Mechanical Sydney Institute of Agriculture and Mechatronic Engineering

Page 22 Page (Australian Technology Park) 23 Page Contact us

Core Research Facilities, Director Sydney Imaging Professor Simon Ringer +61 2 8627 0955 [email protected] Core Research Facilities,

. au Chief Operating Officer Sydney Informatics Hub

. edu Tim Dixon +61 2 8627 6553

sydney +61 2 8627 4815 [email protected] [email protected] Sydney Manufacturing Hub Research and Prototype Foundry +61 2 8627 7226 +61 2 8627 8118 [email protected] [email protected] Sydney Mass Spectrometry Sydney Analytical +61 2 8627 5454 +61 2 8627 6903 [email protected] [email protected] Sydney Microscopy & Microanalysis Sydney Cytometry +61 2 9351 2351 +61 2 9565 6180 [email protected] [email protected] The University of Sydney University The Page 24 Page . au . edu sydney The University of Sydney University The

sydney.edu.au/research/facilities @Sydney_CRF CRICOS 00026A CRICOS

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