HPLC Analysis of Amine Medications on Ascentis® Express C18, 5 Μm

HPLC Analysis of Amine Medications on Ascentis® Express C18, 5 µm Peak Compound 6 Number 1 Maleic Acid 1 5 2 Pseudoephedrine 3 4 3 Scopolamine 2 4 Doxylamine Absorbance @ 220 nm 5 Chlorpheniramine 6 Diphenhydramine 0.0 1.0 2.0 3.0 4.0 5.0 6.0 Time, min. Conditions: column: Ascentis® Express C18, 15 cm x 4.6 mm I.D., 5 µm mobile phase: [A] 50 mM Ammonium formate (0.1% (v/v) Formic acid); [B] 50:50 Methanol:Acetonitrile (Both 0.1% (v/v) Formic acid) gradient: 20% B to 60% B in 6.5 min flow rate: 1 mL/min column temp.: 30 °C detector: UV, 220 nm injection: 3 µL sample: Amine Compounds, varied concentration, 80:20 Mobile phase A:Mobile phase B Description: There are a variety of amine medications, from antihistamines to decongestants, that can be efficiently separated using HPLC. The Ascentis® Express C18 is ideal for these separations, providing good resolution and fast run times. The use of ammonium formate buffer as a mobile phase helps maintain excellent peak shapes for these basic compounds. The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. SigmaAldrich.com © 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. MilliporeSigma, Supelco, and the vibrant M are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources. Lit. No. PBXXXXXXXX XXX Materials: Product Part Number Description 50537-U Ascentis® Express C18, 15 cm x 4.6 mm I.D., 5 µm 70221 Ammonium formate, LC/MS grade 5.33002 Formic acid, LC/MS grade 34860 Methanol 34851 Acetonitrile M0375 Maleic Acid 287636 (1S,2S)-(+)-Pseudoephedrine PHR1470 Scopolamine hydrobromide PHR1420 Doxylamine succinate C3025 (±)-Chlorpheniramine maleate salt D3630 Diphenhydramine hydrochloride The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. SigmaAldrich.com © 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. MilliporeSigma, Supelco, and the vibrant M are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources. Lit. No. PBXXXXXXXX XXX.

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