Product datasheet Anti- E/PPIE Antibody (monoclonal, 7F2) Catalog Number: M08021-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-Cyclophilin E/PPIE Antibody (monoclonal, 7F2) Name PPIE Source Mouse IgG2a Species Reactivity human, mouse, rat Tested Application WB,IHC-P,ICC/IF,FCM Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human Cyclophilin E/PPIE recombinant protein (Position: M1-V301). Purification protein G purified. Observed MW 35KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an which in humans is encoded by the PPIE gene on 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on , has been identified.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-Cyclophilin E/PPIE Antibody (monoclonal, 7F2) Catalog Number: M08021-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis analysis of anti- PPIE using anti- PPIE antibody antibody (M08021-1).The (M08021-1). detected in sample well of each lane paraffin-embedded was loaded with 50ug of section of human sample under reducing placenta tissue. conditions. Biotinylated goat anti- Lane 1: human HEK293 mouse IgG was used as whole cell lysates, secondary antibody. The Lane 2: human K562 tissue section was whole cell lysates, developed using Strepavi Lane 3: human PC-3 din-Biotin-Complex whole cell lysates, (SABC) (Catalog # Lane 4: human CACO-2 SA1021) with DAB as the whole cell lysates. chromogen. Use mouse anti- PPIE 1:1000, probed with a goat anti- mouse IgG- HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PPIE at approximately 35KD. The expected band size for PPIE is at 35KD.

Figure 3. IHC analysis Figure 4. IHC analysis using anti- PPIE antibody using anti- PPIE antibody (M08021-1). detected in (M08021-1). detected in paraffin-embedded paraffin-embedded section of human colonic section of Rat cardiac adenocarcinoma tissue. tissue. Biotinylated goat Biotinylated goat anti- anti-mouse IgG was used mouse IgG was used as as secondary antibody. secondary antibody. The The tissue section was tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC) (Catalog # (SABC) (Catalog # SA1021) with DAB as the SA1021) with DAB as the chromogen. chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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