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Characterization and Deconstruction of Oligosaccharides in Black Liquor from Deacetylation Process of Corn Stover

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Characterization and Deconstruction of Oligosaccharides in Black Liquor from Deacetylation Process of Corn Stover ORIGINAL RESEARCH published: 07 June 2019 doi: 10.3389/fenrg.2019.00054 Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover Wei Wang 1*, Xiaowen Chen 2*, Rui Katahira 2 and Melvin Tucker 2 1 National Renewable Energy Laboratory, Biosciences Center, Golden, CO, United States, 2 National Renewable Energy Laboratory, National Bioenergy Center, Golden, CO, United States The National Renewable Energy Laboratory (NREL) developed low severity Deacetylation and Mechanical Refining (DMR) process under atmospheric pressure, generates highly fermentable, low toxicity sugar syrups and highly reactive lignin streams. The dilute alkali deacetylation step saponifies acetyl groups from the hemicellulosic fraction of biomass into a black liquor waste stream that also contains solubilized acetic, ferulic, p-coumaric acids, lignin-carbohydrate complexes (LCCs), oligosaccharides, and solubilized lignin. Valorization of the soluble components in the deacetylation black liquor waste stream Edited by: requires characterization of the black liquor. Our analyses show that glucan, xylan, Anli Geng, and lignin were three main components in black liquor and the oligosaccharides in Ngee Ann Polytechnic, Singapore the black liquor were mainly dimers and trimers with arabinofuranose branch groups Reviewed by: Diogo M.F. Santos, mainly on the xylooligomers. GPC chromatograms showed that the black liquor also Association of Instituto Superior contained oligomeric lignin moieties with molecular weights ranging between 1,800 and Técnico for Research and ∼10,000 Da. Enzymatic saccharification of the black liquor was conducted based on Development (IST-ID), Portugal Yong Xu, the compositional and structural information. Incorporation of commercial cellulase and Nanjing Forestry University, China hemicellulase enzyme preparations with various accessary enzymes showed up to 57% *Correspondence: hydrolysis of the xylooligosaccharides in the black liquor to monomeric sugars, which Wei Wang [email protected] could be used to improve biorefinery carbon utilization in biological fermentations to Xiaowen Chen improve end product fuel or bioproduct volumes per metric ton of biomass processed. [email protected] This is so far the first study about characterizing DMR black liquor and exploring breaking Specialty section: down the oligosaccharides in black liquor for potential use. This article was submitted to Keywords: deacetylation, black liquor, characterization, xylooligosaccharides, enzymatic saccharification Bioenergy and Biofuels, a section of the journal Frontiers in Energy Research INTRODUCTION Received: 28 February 2019 Accepted: 23 May 2019 The development of renewable liquid fuels from lignocellulosic biomass is considered by Published: 07 June 2019 Environmental Protection Agency (EPA) as one of the most important strategies to cease the global Citation: climate change attributed to Green House Gas (GHG) emissions from fossil fuels combustion. Wang W, Chen X, Katahira R and Lignocellulosic biomass can be converted to ethanol or other drop-in hydrocarbon fuels through Tucker M (2019) Characterization and single bioconversion or hybrid bio/catalytic conversion technologies in downstream processing Deconstruction of Oligosaccharides in Black Liquor From Deacetylation steps using both the sugars and lignin moieties solubilized and fractionated in pre-treatment and Process of Corn Stover. enzymatic hydrolysis (Davis et al., 2013). Among all the pre-treatment processes, the Deacetylation Front. Energy Res. 7:54. and Mechanical Refining (DMR) process developed by National Renewable Energy Laboratory doi: 10.3389/fenrg.2019.00054 (NREL, Golden, Colorado) is a unique atmospheric pre-treatment process that has demonstrated Frontiers in Energy Research | www.frontiersin.org 1 June 2019 | Volume 7 | Article 54 Wang et al. Characterization and Deconstruction of Oligosaccharides in Black Liquor high sugar concentration (>270 g/L fermentable sugars) and exo-1,4-β-xylosidase (EC 3.2.1.37), which successively removes low toxicity sugar syrup production from corn stover, which D-xylose residues from the non-reducing ends of xylo- also led to an ethanol product titer of 86 g/L in followed oligosaccharides and also cleaves xylobiose. Incorporating xylan fermentation test (Chen et al., 2012, 2014, 2016). Basically, backbone cleaving enzymes with accessory enzymes such as in deacetylation step, dilute sodium hydroxide (NaOH) was arabinofuranosidase has shown significant enhancement on the used to catalyze the saponification of the branched acetyl hydrolysis of the hemicelluloses from acid pre-treated/water groups from hemicellulose backbone as well as to trigger extracted hemicelluloses (Sørensen et al., 2007; Hu et al., partial delignification that occurs under boiling point and 2016). For the dissolved hemicellulose from deacetylation low alkali concentration (<0.2 M). Mechanical refining process, the xylan backbone is likely debranched due to further improves the digestibility of deacetylated biomass the saponification, therefore a xylanase cocktail including by increasing the cellulose accessibility with cutting and accessary enzymes maybe necessary for xylan degradation. fibrillation (Jones et al., 2013, 2014; Kim S. M. et al., 2016; Park However, the structures of the hemicellulose in black liquor et al., 2016). The DMR pre-treated biomass has shown good generated from DMR process still remain unknown due digestibility and superb fermentability as reported previously to the uniqueness of deacetylation conditions. To find a (Zhang et al., 2014; Chen et al., 2016; de Assis Castro et al., 2017). tailored xylanase cocktail specifically targeting at the structures In addition to removing acetyl groups on xylan, deacetylation of dissolved hemicellulose, characterization of black liquor also solubilizes a portion of carbohydrates from corn stover especially dissolved oligosaccharides is very necessary for (xylan 2∼10%, glucan ∼1%) (Chen et al., 2012). Compared potential utilization in future. to severe alkaline pre-treatment conditions (Wyman et al., Herein in this work, in order to finally utilize the valuable 2005; Huang et al., 2015; Kim J. S. et al., 2016), deacetylation compounds in black liquor, a comprehensive compositional in DMR process is too mild to cause complete degradation and spectral analyses for characterizing components in the of carbohydrates therefore the solubilized carbohydrates in black liquor was performed. The types of oligosaccharides black liquor are likely in oligomeric form. As deacetylation were characterized and the functional branch groups on targets on acetyl groups on hemicellulose, most of the dissolved xylooligosaccharides were identified. In addition, enzymatic carbohydrates are xylooligosaccharides. So far, there is barely any breakdown of oligosaccharides in black liquor was also study on recycling these oligosaccharides in black liquor from conducted based on the structural information that we collected. DMR process. The dissolved hemicelluloses in black liquor pose All these results will help us to know about the structures of several challenges regarding to the economic and environmental the components in DMR black liquor, to better understand the issues of biorefinery industries, as follows: potential value of black liquor, and also help us to evaluate the possibility of utilizing the oligosaccharides in black liquor via 1) The loss of the hemicellulose from deacetylation lowers enzymatic deconstruction. All presented results here could serve overall sugar and final product yields, leading to higher as a baseline for future assessment of recycling and reusability of production cost (Davis et al., 2013). black liquor from DMR process. 2) The hemicellulose in the black liquor significantly increases the viscosity of the concentrated black liquor after evaporation, causing issues in handling the waste liquor (Chen et al., 2018). MATERIALS AND METHODS 3) The heating value of the hemicellulose is only 13.6 MJ/kg, Black Liquor which is only half of that of lignin (Sjostrom, 1993). Deacetylation of corn stover was performed in a 90-L paddle The potential utilization of lignin for value-added products reactor. The deacetylation and black liquor preparation was as removes the major heating source from the black liquor, described in previous work (Chen et al., 2012, 2018). Briefly, dry making the residual liquor incombustible (Chen et al., 2019). corn stover chips were immersed in NaOH solution heated up to ◦ In this context, for good use of black liquor, utilization of 80 or 92 C and held for 2 h. After deacetylation, the liquor was hemicelluloses has to be considered. In nature, xylooligomers drained through the 8-inch screen located in the bottom port of are polysaccharides that are difficult to be used directly as the paddle mixer. In this study, three black liquor samples were carbon source (Bankeeree et al., 2018), so it is better if we prepared with different NaOH loadings. can first break down it into monomeric sugars thus they can 1. DMR-BL-50:50gNaOHperkgofcornstoverat80◦C (NaOH: be used as carbon source for microbial growth. However, as ∼4 g/L). hemicelluloses from biomass are highly substituted and possibly 2. DMR-BL-70:70gNaOHperkgofcornstoverat92◦C (NaOH: branched with side groups such as arabinose, galactose and ∼5.8 g/L). linked to lignin moieties forming lignin carbohydrate complex 3. DMR-BL-100: 100g NaOH per kg of corn stover at 92◦C (LCC) (Giummarella and Lawoko, 2017), it is very
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