MAD2L2 Monoclonal ANTIBODY

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For Research Use Only MAD2L2 Monoclonal ANTIBODY www.ptgcn.com Catalog Number:67100-1-Ig Basic Information Catalog Number: GenBank Accession Number: CloneNo.: 67100-1-Ig BC015244 2A4C2 Size: GeneID (NCBI): Recommended Dilutions: 1000 μg/ml 10459 WB 1:5000-1:20000 Source: Full Name: IHC 1:500-1:2000 Mouse MAD2 mitotic arrest deficient-like 2 (yeast) Isotype: Calculated MW: IgG2b 211aa,24 kDa Purification Method: Observed MW: Protein A purification 24 kDa Immunogen Catalog Number: AG28233 Applications Tested Applications: Positive Controls: IHC, WB, ELISA WB : HeLa cells; Species Specificity: IHC : human lymphoma tissue; Human, mouse, rat Note-IHC: suggested angen retrieval with TE buﬀer pH 9.0; (*) Alternavely, angen retrieval may be performed with citrate buﬀer pH 6.0 MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second Background Information polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation. Storage: Storage Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under T: 4006900926 E: [email protected] W: ptgcn.com Proteintech Group brand and is not available to purchase from any other manufacturer. Selected Validation Data Various lysates were subjected to SDS PAGE Immunohistochemical analysis of paraffin- followed by western blot with 67100-1-Ig (MAD2L2 embedded human lymphoma tissue slide using antibody) at dilution of 1:20000 incubated at room 67100-1-Ig (MAD2L2 antibody) at dilution of temperature for 1.5 hours 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9)..

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Ubiquitinated Proliferating Cell Nuclear Antigen Activates Translesion DNA Polymerases ␩ and REV1
Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases ␩ and REV1 Parie Garg and Peter M. Burgers* Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, St. Louis, MO 63110 Edited by Jerard Hurwitz, Memorial Sloan–Kettering Cancer Center, New York, NY, and approved November 4, 2005 (received for review July 14, 2005) In response to DNA damage, the Rad6͞Rad18 ubiquitin-conjugat- and requiring additional activation by the Cdc7͞Dbf4 protein ing complex monoubiquitinates the replication clamp proliferating kinase that normally functions in cell cycle progression (5, 12). cell nuclear antigen (PCNA) at Lys-164. Although ubiquitination of It is this complex pathway that mainly contributes to DNA PCNA is recognized as an essential step in initiating postreplication damage induced mutagenesis in eukaryotic cells. Although repair, the mechanistic relevance of this modification has remained normally involved in lagging strand DNA replication, the high- elusive. Here, we describe a robust in vitro system that ubiquiti- fidelity Pol ␦ is also required for damage-induced mutagenesis. nates yeast PCNA specifically on Lys-164. Significantly, only those The functions of Pol ␨ and Rev1 appear to be uniquely confined PCNA clamps that are appropriately loaded around effector DNA to mutagenesis. Pol ␨ is an error-prone DNA polymerase that can by its loader, replication factor C, are ubiquitinated. This observa- bypass damage (13). Rev1 is a deoxycytidyl transferase that tion suggests that, in vitro, only PCNA present at stalled replication shows the highest catalytic activity opposite template guanines forks is ubiquitinated. Ubiquitinated PCNA displays the same and abasic sites (14, 15). Rev1 is primarily responsible for replicative functions as unmodified PCNA.
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Jimmunol.0901240.Full.Pdf
A Critical Role for REV1 in Regulating the Induction of C:G Transitions and A:T Mutations during Ig Gene Hypermutation This information is current as Keiji Masuda, Rika Ouchida, Yingqian Li, Xiang Gao, of September 24, 2021. Hiromi Mori and Ji-Yang Wang J Immunol published online 8 July 2009 http://www.jimmunol.org/content/early/2009/07/08/jimmuno l.0901240 Downloaded from Supplementary http://www.jimmunol.org/content/suppl/2009/07/07/jimmunol.090124 Material 0.DC1 http://www.jimmunol.org/ Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication by guest on September 24, 2021 *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2009 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Published July 8, 2009, doi:10.4049/jimmunol.0901240 The Journal of Immunology A Critical Role for REV1 in Regulating the Induction of C:G Transitions and A:T Mutations during Ig Gene Hypermutation Keiji Masuda,* Rika Ouchida,* Yingqian Li,†* Xiang Gao,† Hiromi Mori,* and Ji-Yang Wang1* REV1 is a deoxycytidyl transferase that catalyzes the incorporation of deoxycytidines opposite deoxyguanines and abasic sites.
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Identification of Conserved Genes Triggering Puberty in European Sea
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Human Pol Ζ Purified with Accessory Subunits Is Active in Translesion DNA Synthesis and Complements Pol Η in Cisplatin Bypass
Human Pol ζ purified with accessory subunits is active in translesion DNA synthesis and complements Pol η in cisplatin bypass Young-Sam Lee1, Mark T. Gregory, and Wei Yang2 Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 Contributed by Wei Yang, December 27, 2013 (sent for review December 5, 2013) DNA polymerase ζ (Pol ζ) is a eukaryotic B-family DNA polymerase a nucleotide opposite either a cis–syn thymine or a 6-4 photo- that specializes in translesion synthesis and is essential for normal product (23). Genetic data indicate that a complete lesion bypass embryogenesis. At a minimum, Pol ζ consists of a catalytic subunit event may require two TLS DNA polymerases (24)—one for Rev3 and an accessory subunit Rev7. Mammalian Rev3 contains nucleotide incorporation opposite a lesion (insertion step) and >3,000 residues and is twice as large as the yeast homolog. To the other for the subsequent primer extension (extension step). date, no vertebrate Pol ζ has been purified for biochemical char- The insertion step of TLS is often accomplished by a Y-family acterization. Here we report purification of a series of human Rev3 polymerase, whose active site is uncommonly large, solvent- deletion constructs expressed in HEK293 cells and identification of exposed, and flexible (25). Studies of another B-family TLS DNA a minimally catalytically active human Pol ζ variant. With a tagged polymerase from Escherichia coli (Pol II) show that it efficiently form of an active Pol ζ variant, we isolated two additional acces- extends a DNA primer after a lesion by looping out the damaged sory subunits of human Pol ζ, PolD2 and PolD3.
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Gene Section Review
Atlas of Genetics and Cytogenetics in Oncology and Haematology OPEN ACCESS JOURNAL AT INIST-CNRS Gene Section Review MAD2L1 (mitotic arrest deficient 2, yeast, human homolog like-1) Elizabeth M. Petty, Kenute Myrie Division of Medical Genetics Departments of Human Genetics and Internal Medicine University of Michigan Medical School 1150 West Medical Center Drive, 4301 MSRB III, Ann Arbor, Michigan 48109- 0638, USA (EMP, KM) Published in Atlas Database: March 2001 Online updated version : http://AtlasGeneticsOncology.org/Genes/MAD2L1ID304.html DOI: 10.4267/2042/37729 This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence. © 2001 Atlas of Genetics and Cytogenetics in Oncology and Haematology Identity Other names: HsMAD2; MAD2; MAD2A HGNC (Hugo): MAD2L1 Shadded boxes (1-5) depict the 5 exons of MAD2L1. The black Location : 4q27 triangle indicates a del A mutation that was found in the CAL51 Local order: As noted on the GM99-GB4 breast cancer cell line. Open triangkes depict the locations of Chromosome 4 map: Position: 548.24 (cR3000) Lod identified sequence variants. Figure is not drawn to scale. score: 1.16 Reference Interval: D4S2945-D4S430 Transcription (115.1-125.1 cM) It is located within the NCBI BAC MAD2L1 has 5 coding exons. No alternative splicing genomic contig: NT_006302.2 which is part of the has been described. Regulation of its transcription in homo sapiens chromosome 4 sequence segment. human cells is currently poorly understood. Note: MAD2L1 was intially (and errounously) mapped by fluorescence in situ hybridization (FISH) to 5q23- Protein q31. Subsequent comprehensive mapping studies using somatic cell hybrid analysis, radiation hybrid (RH) Description mapping, and FISH localized it to 4q27.
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Anti-MAD2L2 Monoclonal Antibody, Clone FQS24768 (DCABH-6886) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use
Anti-MAD2L2 monoclonal antibody, clone FQS24768 (DCABH-6886) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview Rabbit Monoclonal to Mad2L2 Antigen Description Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. Immunogen Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Mad2L2 aa 150 to the C-terminus. The exact sequence is proprietary.Database link: Q9UI95 Isotype IgG Source/Host Rabbit Species Reactivity Mouse, Rat, Human Clone FQS24768 Purity Protein A purified Conjugate Unconjugated Applications IP, ICC/IF, Flow Cyt, IHC-P, WB Positive Control K562, SW480, Hela, Molt-4 cell lysates, human ovarian carcinoma tissue, Hela cells, Format Liquid Size 100 μl Buffer Preservative: 0.01% Sodium azide; Constituents: 59% PBS, 0.05% BSA, 40% Glycerol 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Preservative 0.01% Sodium Azide Storage Store at +4°C short term (1-2 weeks).
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Ubiquitin and Ubiquitin-Like Proteins Are Essential Regulators of DNA Damage Bypass
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