Efficient Professional and Antibody Platforms GPM6B Antibody Basic information: Catalog No.: UPA61091 Source: Rabbit Size: 50ul/100ul Clonality: polyclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: affinity purified by Protein A Useful Information: WB:1:500-2000 Applications: FC:1ug/Test Reactivity: , Mouse, Rat, Pig, Horse Specificity: This antibody recognizes GPM6B protein. Immunogen: KLH conjugated synthetic peptide derived from human GPM6B 1-100/265 May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteo- Description: blasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake. Uniprot: Q13491 Human BiowMW: 29 KDa Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Sample: U251(Human) Cell Lysate at 30 ug Pri- mary: Anti-GPM6B at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 34 kD

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat an- ti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilu- tion: 0.2ug in 100 uL1X PBS containing 0.5% BSA(green).

Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-GPM6B at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 36 kD

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]