1 a micronizer located in Southern of Italy. Micronized peas and sweet white lupin were also subjected to a roll a to subjected also were lupin white sweet and peas Micronized Italy. of Southern in located micronizer a in min 15 for °C 130 of temperature a at heated) (infrared micronized were peas and lupin white Sweet grown. locally were study this for respectively,used Spirale, and Multitalia pea, and lupin white sweet of cultivars The Materials andMethods the determine to conducted was experiment effects ofmicronizationanddehullingpeasweetwhitelupinonperformance pre-layinghens. an Therefore, industry. hen laying effects positive of possibility the open meal of replacement total or partial on concentrate lupin, and pea on published reports Most not affect henperformance. (Moran treatments thermal and 1990) Perez-Lanzac, and (Castanon lupin and pea of levels high that indicate also studies Other problematic. less be to seems diets layers in pea of Inclusion hens. in 2006) al., et (Diaz performance improve to seems seeds treatment of Heat production. egg in reduction a lupin for reported Perez-Maldonado hens. laying and pre-laying in use its on information little very is there animals, monogastric in lupin and pea of use the on research extensive Despite et al pea and lupin have been researched on as protein sources for monogastric animal diets (Diaz years, past the For acids. amino essential some in deficient are and factors anti-nutritional certain contain grains grains. such these two , are As L.) albus ( lupin white sweet and L.) sativum (Pisum Pea proteins, however, isunabletoprovidetheexactamountofaminoacidsrequiredforallanimals. animal production, which will reduce production cost and also be of ecological advantage. A single source of future. European Union is presently pursuing an agro-politics that encourages the use of home-grown legumes in The utilize of legumes as source of protein for the animal feed industry is expected to increase further in the near Introduction Keywords: Sweetlupin,Pea,Micronization,Pre-layinghen and peaseedsimprovesitsdietaryvalueforpre-layinghens. lupin effectsnegative of micronization no furthermore, with and, performance hens pre-laying growth Brown on among experimental groups. It is concluded that lupin seed meal can replace soybean meal protein in diets for Isa observed were ratio conversion feed and intake feed in differences significant trial the of end diets. the the At in the control group. A positive trend was also noticed for weight gain with the inclusion of lupin and pea seed meal the trial. Final weight of pre-laying hens fed experimental including dehulled and micronized diets was higher than during noticed were groups among utilization feed differencesin significant and well diets all accepted Animals diet. control the of source protein only the 115g/kg), (Control, meal soybean of place in (micronized) radiation infrared by processed and dehulled meal seed Spirale) cv L., sativum (Pisum pea of g/kg 200 or Multitalia) cv were middlings wheat durum formulated to be isonitrogenous and isoenergetic and to contain conditioning 120 g/kg of sweet white lupin (Lupinus albus L., on based diets experimental Three hens. pre-laying Brown Isa for A 7-week growth trial was performed to evaluate the effect of lupin and pea seed meal as a protein source in diets Abstract University of Bari, Faculty of Veterinary Medicine, Department of Animal Health and (LUPINUS ALBUS L.) AND PEA (PISUMSATIVUM L.)SEED MEAL AS AN ALTERNATIVE PROTEINSOURCEINDIETS Welfare, 70010Bari, EVALUATION OF DEHULLED AND MICRONIZEDLUPIN ., 2008). Anti-nutritional factorsinthesegrainsincludetrypsinandchymotrypsininhibitorsamongstothers. V. Laudadio 2 University ofBari,DepartmentCropScience,70125 FOR PRE-LAYING HENS [email protected] 1 , V.Tufarelli G. DiModugno Italy 1 (1999) included pea and sweet lupine in diets and diets in lupine sweet and pea included (1999) al. et , M.Dario 1 1 , E.Cazzato 2 , et al., 2006,; Valencia ., 1968) do 1968) al., et

Book of Proceedings 495 Evaluation of Dehulled and Micronized Lupin (Lupinus Albus L.) and Pea (Pisum Sativum L.) Seed Meal as an Alternative Protein Source in Diets for Pre-Laying Hens process before being ground for use. Dehulling was accomplished with the aid of a roller mill and the hulls were separated from the cotyledons by air classification. Seventy-five Isa Brown pullets were equally divided into three groups and used to evaluate the feeding value of micronized and dehulled peas and sweet white lupin in a 7-weeks experiment, between the 11- to 18-weeks of age (mean body weight 940 ± 0.87 g). The birds were housed in colony cages (40 × 25 cm) at two bird per cage. The cages were equipped with cup waterers and trough feeders. The hens were housed in an environmentally

Book of Proceedings controlled house. Three similar isoenergetic and isonitrogenous diets were formulated and the chemical compositions of micronized

Mediterraean Summit of WPSA Mediterraean Summit of and dehulled pea and sweet white lupin diets have been reported (Table 1). The inclusion level of sweet white nd

2 lupin in diet was 120 g/kg, peas 200 g/kg and the soybean diet (Soybean diet) was 115 g/kg. Live body weight and gain were recorded weekly for each treatment. Feed consumption was determined on a replicate basis by 496 weighing feed at the beginning and at the end of each period. Pullets were weighed individually at the start and at the end of the experiment. Mortality was recorded as it occurred. Feed conversion ratio were calculated from the data. Data were subjected to analysis of variance using the general linear models (GLM) procedure of SAS (SAS Institute, 1990). Treatment differences obtained upon statistical analyses were compared using Duncan’s multiple range test. Results and Discussion Table 2 shows the live body weight, body weight gain, feed intake, feed conversion ratio and mortality of pre- laying pullets fed different protein sources between 11- to 18- weeks of age. In the pre-laying phase (11-18 weeks of age), dietary main protein source had significant effect on feed intake (P <0.05), in particular pullets fed dehulled and micronized lupin and pea consumed more feed compared to the traditions control soybean diet that significantly reduced feed intake. Body weight at 18 weeks of age was unaffected by alternative dietary protein content but there was a positive higher trend for birds fed experimental diets containing lupin or pea seeds. Consequently, body weight gain of pre-laying hens fed the different diet did not significantly vary, even if a positive gain was reported for diets containing lupin or pea seeds. Between 11 and 18 weeks of age, feed conversion ratio of pre-laying hens, in terms of feed intake per unit body weight gain, was marginally reduced (P < 0.05) on the soybean diet but not on the lupin and pea diet (6.66 vs. 6.85 and 7.27, respectively). During the experimental period no case of mortality was recorded among dietary treatments. In conclusion, dehulled and micronized sweet white lupin and pea seeds result a suitable dietary protein source for pullets in pre-laying phase that can fully replace traditionally used protein sources like soybean without adverse effects on the growth performance parameters. 1 Table 2.Effect ofexperimentaldietsongrowthperformancepre-layinghens(betweenthe11- to18-weeksofage). 2 Co; 15,000mgZn;200I;20Se.. mg 40 Mn; mg 30,000 Cu; mg 1,000 Fe; mg 10,000 D-biotin; mg 20 acid; Folic mg 150 Vitmg B12; 3 Vit B6; Vitmg 500 E; V 1 Table 1.Compositionandchemicalanalysisofexperimentaldiets(%asfedunlessotherwisestated) SE, standarderror. a-b, Meanswithinacolumnwith different superscriptdiffer significantly( 1,4-beta-xylanase 2,600,000U Provided/kg of product: 2,500.000 IU Vit A; 300,000 IU Vit D3; 7,500 mcg 25-hydroxycholecalciferol; 6,000 mg Provided/kg of product: Endo-1,4-beta-glucanase, 800,000 U; Endo-1,3(4)-beta-glucanase 1,800,000 U; Endo- U; 1,800,000 Endo-1,3(4)-beta-glucanase U; 800,000 Endo-1,4-beta-glucanase, product: of Provided/kg not det ME, kcal/kg Chemical analysis,% Ingredients, % Dry matter Durum wheatmiddlings Choline chloride Enzyme Crude protein Durum wheatbran Crude fiber Pea Ether extract Lupin Ash Soybean meal,44%CP Calcium Calcium carbonate Available P Soybean oil Sodium Dicalcium phosphate Lys Sodium chloride Met +Cys Sodium dicarbonate Yeast DL-Met L-Lys Vitamin-Mineral premix Treatment Soybean diet Lupin diet Pea diet ±SE ected 2 it K3; 4,000 mg 4,000 K3; it Vitmg 300 PP; Vitmg 1,000 B1; Vitmg 400 acid; D-pantothenic mg 2,000 B2; 1 . 2702 Soybean diet 0.10 0.40 90.88 68.82 0.10 17.05 10.00 3.68 - 4.51 - 8.16 11.50 2.08 3.70 0.45 2.20 0.17 2.15 0.80 0.20 0.65 0.20 0.10 0.16 0.37 Live body weight (g, at18weeks) 1504 1564 1552 5.39 Body weight gain (g/day) 11.51 12.78 12.49 0.04 2719 Lupin diet 0.10 0.40 91.06 63.65 0.10 17.11 14.55 3.42 - 4.33 12.00 8.20 - 2.07 3.80 0.45 2.00 0.17 2.00 0.80 0.20 0.65 0.20 0.10 0.35 0.55 P < 0.05). Feed intake (g/day) 62.72a 71.99b 71.31b 0.13 FCR (g/g) 6.66a 6.85ab 7.27b 0.03 2702 Pea diet 0.10 0.40 90.92 55.70 0.10 17.02 14.55 3.11 20.00 3.30 - 7.98 - 2.08 3.80 0.45 2.20 0.17 2.05 0.81 0.20 0.65 0.20 0.10 0.25 0.35 Mortality (%) nd1 nd nd

Book of Proceedings 497 Evaluation of Dehulled and Micronized Lupin (Lupinus Albus L.) and Pea (Pisum Sativum L.) Seed Meal as an Alternative Protein Source in Diets for Pre-Laying Hens Book of Proceedings 2nd Mediterraean Summit of WPSA VALENCIA, D.G., SERRANO, M.P., CENTENO, C., LÁZARO, R. and MATEOS, G.G. (2008) Pea protein as a substitute of SAS Institute(1990)User’s GuideStatistics5thed.SASInstitute.Cary. NC. P.F.Mannion, and R.A., Perez-Maldonado, and J.D. Summers, E.T., Moran, , G. Fusconi, M., oschini, M F., Masoero, M., Morlacchini, D., Diaz, and CastanonJ.I.R. , References diets: effect ofextrusionongrowthperformance. sativum), faba (Vicia faba var. minor) and lupin seeds (Lupinus albus var. multitalia) as protein sources in broiler soya beanproteinindietsforyoungpigs:Effects onproductivityanddigestivetraits. 40: 667-673. experiments. layer and composition Chemical I. diets. poultry in lupins sweet and peas chick Canadian Journalof Animal Science value. nutritional improve to means practical a as pelleting steam temperature high on emphasis with hen laying and laying Leghornhens. faba), sweet lupins (Lupinus albus), cull peas Pisum ( sativum) and vetches (Vicia sativa) in diets for high performance Perez-Lanza, British PoultryScience (1968) Field pea a major dietary protein source for the growing chick growing the for source protein dietary major a pea Field (1968) G.E. Jones, (1990) Substitution of fixed amounts of soybean meal for field beans (Viciabeans field for meal soybean of amounts fixed of Substitution (1990) c.J. 48:47-55. 31:173-180. F (1999) Optimum inclusion of field peas, faba beans, faba peas, field of inclusion Optimum (1999) D.J. arrell, Italian Journalof Animal Science (2006) Pea seeds (Pisum seeds Pea (2006) PivaG. and , 5:43-53. Livestock Science British Poultry Science Poultry British 118: 1-10.