Monoclonal Antibody to LGI1 / EPT (37-113) - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AM60048PU-N Monoclonal Antibody to LGI1 / EPT (37-113) - Purified

Alternate names: Epitempin-1, Leucine-rich glioma-inactivated protein 1 Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist. Uniprot ID: Q9JIA1 NCBI: NP_064674.1 GeneID: 56839 Host / Isotype: Mouse / IgG2a Recommended Isotype AM03096PU-N Controls: Clone: S283-7 Immunogen: Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1 /EPT, SwissProt: Q9JIA1.1. Format: State: Liquid purified IgG fraction Purification: Protein G chromatography Buffer System: PBS pH 7.4, 50% Glycerol, 0,09% Sodium azide Applications: Western blot: 1/1000; 1μg/ml was sufficient for detection of LGI1 / EPT in 20 μg of rat brain lysate by colorimetric immunoblot using HRP conjugated secondary antibody. Immunocytochemistry. Immunohistochemistr: Free floating sections, fixed in formaldehyde. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody detects a 60 kDa protein corresponding to the molecular weight of LGI1 / EPT. It will recognize only the longer form, Isoform 1. (~50% identity with LGI2, LGI3 and LGI4.) Species Reactivity: Tested: Mouse, Rat. Expected from sequence similarity: Human (98%). Storage: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. BK/20140206 1 / 2 AM60048PU-N: Monoclonal Antibody to LGI1 / EPT (37-113) - Purified

General Readings: 1. Chernova OB, Somerville RP, Cowell JK. A novel gene, LGI1, from 10q24 is rearranged and downregulated in malignant brain tumors. Oncogene. 1998 Dec 3;17(22):2873-81. PubMed PMID: 9879993. 2. Fialka F, Gruber RM, Hitt R, Opitz L, Brunner E, Schliephake H, et al. CPA6, FMO2, LGI1, SIAT1 and TNC are differentially expressed in early- and late-stage oral squamous cell carcinoma--a pilot study. Oral Oncol. 2008 Oct;44(10):941-8. doi: 10.1016/j.oraloncology.2007.10.011. Epub 2008 Jan 29. PubMed PMID: 18234543. 3. Gu W, Gibert Y, Wirth T, Elischer A, Bloch W, Meyer A, et al. Using gene-history and expression analyses to assess the involvement of LGI genes in human disorders. Mol Biol Evol. 2005 Nov;22(11):2209-16. Epub 2005 Jul 13. PubMed PMID: 16014869. 4. Mironov OL, Bilobrov VM, Kovrizhkina OS, Shtoda LA. [The use of trimethine dye to stain proteins following cellulose acetate electrophoresis]. Lab Delo. 1990;(6):9-11. PubMed PMID: 1699055.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. BK/20140206 2 / 2