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Biobutanol Production from Hexose and Pentose Sugars

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Biobutanol Production from Hexose and Pentose Sugars 193 A publication of CHEMICAL ENGINEERING TRANSACTIONS The Italian Association VOL. 38, 2014 of Chemical Engineering www.aidic.it/cet Guest Editors: Enrico Bardone, Marco Bravi, Taj Keshavarz Copyright © 2014, AIDIC Servizi S.r.l., ISBN 978-88-95608-29-7; ISSN 2283-9216 DOI: 10.3303/CET1438033 Biobutanol Production from Hexose and Pentose Sugars a a a,b,* Francesca Raganati , Alessandra Procentese , Giuseppe Olivieri , Piero Salatino a, Antonio Marzocchellaa aDipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriali - Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli – Italy bBioprocess Engineering, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, the Netherlands [email protected] The Acetone-Butanol-Ethanol (ABE) fermentation is receiving renewed interest as a way to upgrade renewable resources for the production of products with high added value as chemicals and fuels. Main pre-requisites of fermentation feedstocks are abundance and un-competitiveness with food sources and they are fulfilled by lignocellulosic biomass. This contribution reports about the characterization of the ABE fermentation by Clostridium acetobutylicum DSM 792 adopting sugars representative for hydrolysis products of lignocellulosic biomass: glucose, mannose, arabinose, and xylose. Batch fermentation tests with binary mixtures of sugars were performed to assess the possible crossed/coupled effects of the investigated sugars on the fermentation performances. The mass ratio of sugars in binary mixture tests was set at 1:1 and the total initial concentration was set at 60 g/L. The conversion process was characterized as a function of the time in terms of biomass, acids, and solvents concentrations as well as of pH and total organic compounds. The simultaneously fermentation of binary mixture of sugars enhances the conversion of the investigated sugars into butanol/solvents. The xylose fermentation appears to be improved when it is mixed with the investigated sugars. 1. Introduction The interest in biofuel production is continuously increasing in the last decades for various reasons, among which: the exhaustion of worldwide oil deposits, the awareness in greenhouse gas emissions during combustion of fossil fuels, and the impacts of the anthropic activity on global warming. Butanol produced via ABE fermentation from renewable feedstocks using Clostridium acetobutylicum is a potential candidate as a biofuel. Main features of butanol are: low vapour pressure, blending with both gasoline and diesel at any fraction, energy density close to that of the gasoline, fuelled to current configuration of engines without any retrofitting, managing in the current infrastructures (Cascone, 2008). Butanol is currently produced from the petroleum according to the Oxo process (reaction of alkenes with carbon monoxide and hydrogen). It may also be produced from renewable resources (biomass) by the acetone-butanol-ethanol (ABE) fermentation. The main actors of this fermentation process are the clostridia (saccharolytic butyric acid-producing bacteria). Several strains are capable to produce significant amounts of neutral solvents during the later stage of batch fermentations under selected operating conditions (Clostridium saccharoperbutylacetonicum, C. acetobutylicum, C. beijerinckii, C. aurantibutyricum). Solventogenic clostridia are anaerobic strains able to metabolize a great variety of substrates: pentoses, hexoses, mono-, di- and polysaccharides (Flickinger and Drew, 1999). Typically, the strains selected in industrial fermentation depend on the nature of the raw material available on the market, the required ratio between the solvents, the need for additional nutrients and phage resistance (Ross, 1961). C. acetobutylicum has been successfully adopted for the production of acetone and butanol. Under batch conditions the fermentation process of solvent-producing clostridium strains starts with the production of cells, hydrogen, carbon dioxide, acetic acid and butyric acid during the initial growth phase (acidogenesis). As the acid concentrations increase (pH decrease), the metabolism of cells shifts to solvent production (solventogenesis) and the acidogenic cells – able to reproduce themselves - shift to the Please cite this article as: Raganati F., Procentese A., Olivieri G., Salatino P., Marzocchella A., 2014, Biobutanol production from hexose and pentose sugars, Chemical Engineering Transactions, 38, 193-198 DOI: 10.3303/CET1438033 194 solventogenesis state with a morphological change. During solventogenesis the active cells become endospores unable to reproduce themselves. Two different physiological states must be taken into account for Clostridia: one for the acidogenic phase, and one for the solventogenic phase (Jones and Woods, 1986). The fermentation substrate plays a fundamental role in the cost of butanol production. The possibility to select feedstocks plentiful, inexpensive, and not edible is the success key for any process voted to produce biofuels from renewable resources (Qureshi and Blaschek, 2000; Kumar and Gayen, 2011; Raganati et al., 2013a). Lignocellulose is the most plentiful renewable resource on the planet, it is made of potential fermentable sugars, and it is not useful as food resources. Despite the advantages in sustainability and availability, processes based on lignocellulosic biomass are still far to be commercialized. Main obstacles for process commercialization are the pre-treatment steps and the fermentation of sugars mixtures produced during the pre-treatments. Apart from the mechanical comminution of the biomass, particular attention is paid at the hydrolysis of hemicellulose and cellulose that produces mixtures of five- and six-carbon sugars. Moreover, the fermentation of a such reach sugar mixtures is not an easy task because yield and kinetics related to the components of these mixtures range over wide spectra (Raganati et al., 2012; Qureshi, 2007). Typically, diauxic fermentation is observed when glucose and other sugars are present in the feedstock: the latter sugars are converted after glucose depletion. From an economic point of view, it would be preferred that pentose and hexose sugars are converted simultaneously (Ezeji et al., 2003). Although several scientific contributions are available in literature on the ability of Clostridia to produce ABE by conversion of sugars obtained from lignocellulosic biomass (Ezeji and Blaschek, 2008), an exhaustive systematic investigation has not been carried out yet. A research program is active in Napoli regarding production of butanol by ABE fermentation (Napoli et al., 2010; Napoli et al, 2012; Raganati et al., 2013). First attempts to characterize the ABE production by Clostridium acetobutylicum DSM 792 fermentation of sugars typically present in lignocellulosic biomass hydrolysed have been reported (Raganati et al., 2012). Preliminary tests carried out according to the procedure reported by Napoli et al. (2009) were realized adopting single sugars - glucose, mannose, arabinose, and xylose - as carbon source to investigate the ability of the microorganism to convert them. Previously, we examined the effect of each individual sugar on C. acetobutylicum growth and butanol production (Raganati et al., 2013b). These preliminary batch fermentation tests with single sugars were aimed to assess the ability of C. acetobutylicum to ferment sugars typically present in the hydrolyzed fraction of lignocellulosic biomass. The tested sugars included glucose, mannose, arabinose, and xylose. The initial sugar concentration was 60 g/L (before inoculation) in all experiments. Main results of the preliminary tests are reported in Table 1. It is evident that: i) C. acetobutylicum was able to convert the sugars typically present in lignocellulosic biomass hydrolysates into solvents (ABE); ii) glucose was confirmed as the sugar characterized by the best performance (13.2 g/L of butanol); iii) the fermentation performances of the other sugars decreased with the order mannose, arabinose and xylose. Table 1 also shows that the residual acid concentration depended on the sugar species. In particular, the residual acid concentration was very high during tests with mannose, arabinose and xylose and the ABE yield decreased significantly in these tests with respect to the glucose fermentation. These observations suggest an higher capacity of C. acetobutylicum to uptake and recycle the acids when glucose was the carbon source. Table 1: Fermentation tests of C. acetobutylicum. The reported data are the maximum value measured during the fermentation tests with single sugars (60 g/L) (after Raganati et al., 2012). hexoses pentoses Glucose Mannose Arabinose Xylose Cell (gDM/L) 3.91 3.03 2.66 4.28 Cell yield (gDM/g) 0.07 0.05 0.05 0.10 Butanol (g/L) 13.19 8.91 8.7 7.82 This contribution moves a step further along this activity and reported results on the fermentation of mixtures of couple of sugars to assess the crossed/coupled effects of the investigated sugars on the fermentation performances. The total initial sugar concentration was set at 60 g/L. The mass ratio of sugars in binary mixture tests was set at 1:1. The conversion process was characterized as a function of the time in terms of biomass, acids, solvents concentrations and pH. Data were processed to assess sugar conversion and the kinetics of cell growth and of the ABE production. The yields of cells, acids and solvents on the carbon source were also assessed. 195 2. Materials and methods 2.1
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