WNT3, ID (WNT3, INT4, Proto-oncogene Wnt-3, Proto- oncogene Int-4 homolog) (AP)

Catalog number 043890-AP Supplier United States Biological

The WNT family consists of structurally related which encode secreted signaling . These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the 17q21 region. Applications Suitable for use in Western Blot, ELISA Recommended Dilution ELISA: 1:1,000 Western Blot: 1:100-500 Storage and Stability Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note Applications are based on unconjugated antibody. Immunogen KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 155-184 in the central region of human WNT3, UniProt Accession #P56703. Species : chicken, mouse, xenopus Formulation Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity Purified by Protein A and peptide affinity chromatography. Specificity Recognizes human WNT3. Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications E WB Crossreactivity Hu Storage 4°C Do Not Freeze Detection Method AP Reference Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010) Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010) Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010) Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010) Memarian, A., et al. Leuk. Lymphoma 50(12):2061-2070(2009)

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