SANTA CRUZ BIOTECHNOLOGY, INC. TMEM51 shRNA Plasmid (m): sc-154476-SH

BACKGROUND PRODUCT TMEM51 (transmembrane protein 51) is a 175 amino acid protein encoded TMEM51 shRNA Plasmid (m) is a pool of 3 target-specific lentiviral vector by a mapping to human 1. is the largest plasmids each encoding 19-25 nt (plus hairpin) shRNAs designed to knock human chromosome spanning about 260 million base pairs and making up down gene expression. Each plasmid contains a puromycin resistance gene 8% of the . There are about 3,000 on chromosome 1, for the selection of cells stably expressing shRNA. Each vial contains 20 µg and considering the great number of genes there are also a large number of of lyophilized shRNA plasmid DNA. Suitable for up to 20 transfections. Also diseases associated with chromosome 1. Notably, the rare aging disease see TMEM51 siRNA (m): sc-154476 and TMEM51 shRNA (m) Lentiviral Hutchinson-Gilford progeria is associated with the LMNA gene which Particles: sc-154476-V as alternate gene silencing products. encodes Lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The mechanism of STORAGE AND RESUSPENSION rapidly enhanced aging is unclear and is a topic of continuing exploration. Store lyophilized shRNA plasmid DNA at 4° C with desiccant. Stable for The MUTYH gene is located on chromosome 1 and is partially responsible at least one year from the date of shipment. Once resuspended, store at for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher 4° C for short term storage or -80° C for long term storage. Avoid repeated disease and Usher syndrome are also associated with chromosome 1. A freeze thaw cycles. breakpoint has been identified in 1q which disrupts the DISC1 gene and is linked to schizophrenia. Aberrations in chromosome 1 are found in a variety Resuspend lyophilized shRNA plasmid DNA in 200 µl of the deionized water of cancers including head and neck cancer, malignant melanoma and multi- provided. Resuspension of the shRNA plasmid DNA in 200 µl of deionized ple myeloma. water makes a 0.1 µg/µl solution in a 10 mM Tris, 1 mM EDTA buffered solution. REFERENCES APPLICATIONS 1. Watson, M.L., et al. 1990. Genomic organization of the selectin family of leukocyte adhesion molecules on human and mouse chromosome 1. J. TMEM51 shRNA Plasmid (m) is recommended for the inhibition of TMEM51 Exp. Med. 172: 263-272. expression in mouse cells.

2. Blackwood, D.H., et al. 2001. Schizophrenia and affective disorders— SUPPORT REAGENTS cosegregation with a translocation at chromosome 1q42 that directly dis- rupts brain-expressed genes: clinical and P300 findings in a family. Am. J. For optimal shRNA Plasmid transfection efficiency, Santa Cruz Biotechnology’s Hum. Genet. 69: 428-433. shRNA Plasmid Transfection Reagent: sc-108061 (0.2 ml) and shRNA Plasmid Transfection Medium: sc-108062 (20 ml) are recommended. Control shRNAs 3. Weise, A., et al. 2005. New insights into the evolution of chromosome 1. are available as 20 µg lyophilized plasmid DNA. Each encodes a scrambled Cytogenet. Genome Res. 108: 217-222. shRNA sequence that will not lead to the specific degradation of any known 4. Lans, H. and Hoeijmakers, J.H. 2006. Cell biology: aging nucleus gets out cellular mRNA. Control shRNA Plasmids include: sc-108060, sc-108065 and of shape. Nature 440: 32-34. sc-108066.

5. Hennah, W., et al. 2006. Genes and schizophrenia: beyond schizophrenia: RT-PCR REAGENTS the role of DISC1 in major mental illness. Schizophr. Bull. 32: 409-416. Semi-quantitative RT-PCR may be performed to monitor TMEM51 gene 6. Gregory, S.G., et al. 2006. The DNA sequence and biological annotation of expression knockdown using RT-PCR Primer: TMEM51 (m)-PR: sc-154476-PR human chromosome 1. Nature 441: 315-321. (20 µl). Annealing temperature for the primers should be 55-60° C and the 7. Marzin, Y., et al. 2006. Chromosome 1 abnormalities in multiple myeloma. extension temperature should be 68-72° C. Anticancer Res. 26: 953-959. RESEARCH USE 8. McClintock, D., et al. 2006. Hutchinson-Gilford progeria mutant lamin A primarily targets human vascular cells as detected by an anti-Lamin A The purchase of this product conveys to the buyer the nontransferable right G608G antibody. Proc. Natl. Acad. Sci. USA 103: 2154-2159. to use the purchased amount of the product and all replicates and derivatives for research purposes conducted by the buyer in his laboratory only (whether 9. Scaffidi, P. and Misteli, T. 2006. Lamin A-dependent nuclear defects in the buyer is an academic or for-profit entity). The buyer cannot sell or other- human aging. Science 312: 1059-1063. wise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party, or otherwise use this product CHROMOSOMAL LOCATION or its components or materials made using this product or its components Genetic locus: Tmem51 (mouse) mapping to 4 E1. for Commercial Purposes.

PROTOCOLS See our web site at www.scbt.com for detailed protocols and support products.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com