Monoclonal Anti-VPS4A, Clone VPS4-110

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Anti-VPS4A antibody, Mouse monoclonal clone VPS4-110, purified from hybridoma cell culture Product Number SAB4200215 Product Description Reagent Monoclonal Anti-VPS4A (mouse IgG1 isotype) is Supplied as a solution in 0.01 M phosphate buffered derived from the hybridoma VPS4-110 produced by saline, pH 7.4, containing 15 mM sodium azide as a the fusion of mouse myeloma cells and splenocytes preservative. from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human VPS4A Antibody concentration: 1.0 mg/mL (GeneID: 27183), conjugated to KLH. The corresponding sequence is identical in mouse and rat Precautions and Disclaimer and differs by 3 amino acids in human VPS4B.The For R&D use only. Not for drug, household, or other isotype is determined by ELISA using Mouse uses. Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The antibody is purified from culture Storage/Stability supernatant of hybridoma cells grown in a bioreactor. For continuous use, store at 2–8 C for up to one month. For extended storage, freeze at –20 oC in Monoclonal Anti-VPS4A recognizes human, mouse, working aliquots. Repeated freezing and thawing, or rat, bovine, and canine VPS4A. The antibody may be storage in “frost-free” freezers, is not recommended. If used in several immunochemical techniques including slight turbidity occurs upon prolonged storage, clarify immunoblotting (50 kDa) and immunoprecipitation. the solution by centrifugation before use. Working Detection of the VPS4A band by immunoblotting is dilution samples should be discarded if not used within specifically inhibited by the immunizing peptide. 12 hours. Vps4A is a member of the AAA protein family Product Profile (ATPases associated with diverse cellular activities), Immunoblotting: a working antibody concentration of and is the homolog of the yeast Vps4 protein. In 1-2 g/mL is recommended using whole extracts of humans, two paralogs of the yeast protein have been mouse 3T3 or human HEK-293 cells. identified, Vps4A and Vps4B, that share a high degree of amino acid sequence similarity with each other, and Note: In order to obtain best results in various also with yeast Vps4 and mouse Skd1 proteins. The techniques and preparations, it is recommended to mouse Skd1 (suppressor of K+ transport defect 1) has determine optimal working dilutions by titration. been shown to be actually a yeast Vps4 ortholog. References Functional studies indicate that both human paralogs 1. Yoshimori, T., et al., Mol. Biol. Cell, 11, 747-763 associate with the endosomal compartments, and are (2000). involved in intracellular protein trafficking, similar to 2. Stuchell-Brereton, M.D., et al., Nature, 449, 740- Vps4 protein in yeast. Vps4 mediates endosomal 744 (2007). membrane protein sorting, retroviral budding, and 3. Lata, S., et al., Science, 321, 1354-1357 (2008). cytokinesis by recognizing membrane-associated 4. Obita, T., et al., Nature, 449, 735-739 (2007). ESCRT-III assemblies and catalyzing their 5. Wollert, T., et al., Nature, 458, 172-177 (2009). disassembly. The gene encoding Vps4A has been mapped to chromosome 16 while the gene for Vps4B RC,VS,ST,KAA,PHC,MAM 12/20-1 1-5 resides on chromosome 18. ©2020 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip. .

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Anti-VPS4A Antibody (ARG43067)
Product datasheet [email protected] ARG43067 Package: 50 μg anti-VPS4A antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes VPS4A Tested Reactivity Hu, Ms, Rat Tested Application FACS, IHC-P, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name VPS4A Antigen Species Human Immunogen Recombinant protein corresponding to M1-K71 of Human VPS4A. Conjugation Un-conjugated Alternate Names SKD2; SKD1; hVPS4; Protein SKD2; SKD1A; Vacuolar protein sorting-associated protein 4A; VPS4-1; EC 3.6.4.6; VPS4 Application Instructions Application table Application Dilution FACS 1:150 - 1:500 IHC-P 1:200 - 1:1000 WB 1:500 - 1:2000 Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 49 kDa Properties Form Liquid Purification Affinity purification with immunogen. Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. Preservative 0.05% Sodium azide Stabilizer 4% Trehalose Concentration 0.5 mg/ml Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed www.arigobio.com 1/3 before use. Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol VPS4A Gene Full Name vacuolar protein sorting 4 homolog A (S.
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VPS4A (NM 013245) Human Tagged ORF Clone Lentiviral Particle Product Data
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The Vacuolar Protein Sorting Genes in Insects: a Comparative Genome View
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Data Sheet for and VPS4B
Anti-VPS4 produced in rabbit, affinity isolated antibody Product Number SAB4200025 Product Description Reagent Anti-VPS4 is produced in rabbit using as the Supplied as a solution in 0.01 M phosphate buffered immunogen a synthetic peptide corresponding to a saline, pH 7.4, containing 15 mM sodium azide as a fragment of human VPS4A (GeneID: 27183), preservative. conjugated to KLH. The corresponding sequence is identical in mouse and rat, and differs by 3 amino acids Antibody concentration: 1.0 mg/mL in human VPS4B. The antibody is affinity-purified using the immunizing peptide immobilized on agarose. Precautions and Disclaimer For R&D use only. Not for drug, household, or other Anti-VPS4 recognizes human, mouse, and rat VPS4A uses. Please consult the Safety Data Sheet for and VPS4B. The antibody may be used in various information regarding hazards and safe handling immunochemical techniques including immunoblotting practices. (50 kDa) and immunoprecipitation. Detection of the VPS4A and VPS4B bands by immunoblotting is Storage/Stability specifically inhibited by the immunizing peptide. Store at –20 C. For continuous use, the product may be stored at 2–8 C for up to one month. For extended Vps4A is a member of the AAA protein family (ATPases storage, freeze in working aliquots at –20 C. Repeated associated with diverse cellular activities) and is the freezing and thawing is not recommended. If slight homolog of the yeast Vps4 protein. In humans, two turbidity occurs upon prolonged storage, clarify the paralogs of the yeast protein have been identified, solution by centrifugation before use. Working dilution Vps4A and Vps4B, that share a high degree of amino samples should be discarded if not used within acid sequence similarity with each other, and also with 12 hours.
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Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance Eiji Moritaa, Leremy A. Colfa, Mary Anne Karrena, Virginie Sandrina, Christopher K. Rodeschb, and Wesley I. Sundquista,1 aDepartment of Biochemistry and bFluorescence Microscopy Core Facility, University of Utah School of Medicine, Salt Lake City, UT 84112-5650 Edited by Robert A. Lamb, Northwestern University, Evanston, IL, and approved June 7, 2010 (received for review April 30, 2010) The ESCRT pathway helps mediate the ﬁnal abscission step of cyto- for abscission. This translocation event is one of many emerging kinesis in mammals and archaea. In mammals, two early acting pro- links between centrosomes and cytokinesis (reviewed in ref. 19). teins of the ESCRT pathway, ALIX and TSG101, are recruited to the Indeed, it has been proposed that centrosomes may regulate midbody through direct interactions with the phosphoprotein mammalian pathway(s) that help control the transition from mi- CEP55. CEP55 resides at the centrosome through most of the cell tosis to cytokinesis, as is the case for the “mitotic exit network,” cycle but then migrates to the midbody at the start of cytokinesis, which is organized by yeast spindle pole bodies (20). CEP55 binds suggesting that the ESCRT pathway may also have centrosomal and recruits the early-acting ALIX and ESCRT-I proteins to the links. Here, we have systematically analyzed the requirements for midbody, and these interactions are required for efﬁcient abscis- late-acting mammalian ESCRT-III and VPS4 proteins at different sion (3, 4, 21). The roles of late-acting ESCRT factors in cytoki- stages of mitosis and cell division.
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UC Berkeley UC Berkeley Electronic Theses and Dissertations
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Human and Mouse Essentiality Screens As a Resource for Disease Gene Discovery
The Jackson Laboratory The Mouseion at the JAXlibrary Faculty Research 2020 Faculty Research 1-31-2020 Human and mouse essentiality screens as a resource for disease gene discovery. Pilar Cacheiro Violeta Muñoz-Fuentes Stephen A. Murray Mary E Dickinson Maja Bucan See next page for additional authors Follow this and additional works at: https://mouseion.jax.org/stfb2020 Part of the Life Sciences Commons, and the Medicine and Health Sciences Commons Authors Pilar Cacheiro, Violeta Muñoz-Fuentes, Stephen A. Murray, Mary E Dickinson, Maja Bucan, Lauryl M J Nutter, Kevin A Peterson, Hamed Haselimashhadi, Ann M Flenniken, Hugh Morgan, Henrik Westerberg, Tomasz Konopka, Chih-Wei Hsu, Audrey Christiansen, Denise G Lanza, Arthur L Beaudet, Jason D Heaney, Helmut Fuchs, Valerie Gailus-Durner, Tania Sorg, Jan Prochazka, Vendula Novosadova, Christopher J Lelliott, Hannah Wardle-Jones, Sara Wells, Lydia Teboul, Heather Cater, Michelle Stewart, Tertius Hough, Wolfgang Wurst, Radislav Sedlacek, David J Adams, John R Seavitt, Glauco Tocchini-Valentini, Fabio Mammano, Robert E Braun, Colin McKerlie, Yann Herault, Martin Hrabě de Angelis, Ann-Marie Mallon, K C Kent Lloyd, Steve D M Brown, Helen Parkinson, Terrence F Meehan, Damian Smedley, Genomics England Research Consortium, and International Mouse Phenotyping Consortium ARTICLE https://doi.org/10.1038/s41467-020-14284-2 OPEN Human and mouse essentiality screens as a resource for disease gene discovery Pilar Cacheiro et al.# The identiﬁcation of causal variants in sequencing studies remains a considerable challenge that can be partially addressed by new gene-speciﬁc knowledge. Here, we integrate measures of how essential a gene is to supporting life, as inferred from viability and phenotyping 1234567890():,; screens performed on knockout mice by the International Mouse Phenotyping Consortium and essentiality screens carried out on human cell lines.
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New Genetic Vulnerability Found in Upwards of a Third of All Cancers 16 December 2020, by Tom Ulrich
New genetic vulnerability found in upwards of a third of all cancers 16 December 2020, by Tom Ulrich deleted or turned off. Such genes normally prevent damaged cells from growing unchecked. "Tumor suppressors are hard to target therapeutically," said Brenton Paolella, a research scientist with the DepMap team and the Broad Cancer Program and Dana-Farber, and co-first author of the study with postdoctoral fellow Jasper Neggers. "But we realized we had an opportunity to find new targets by looking for other genes associated with tumor suppressor loss." The researchers turned their attention to VPS4A and VPS4B after examining cell lines that had lost one of two tumor suppressor genes, CDH1 and SMAD4, that are commonly deleted in cancer. They found that VPS4A is often deleted along with Credit : Lauren Solomon, Broad Communications CDH1, which sits close by it on chromosome 16. They also discovered a similar relationship between VPS4B with SMAD4, which occupy the same neighborhood on chromosome 18. Cancer mutations often incapacitate critically important genes, forcing tumors to depend heavily Interestingly, the research team noted that cell lines on other genes for survival. Such genetic representing a wide variety of cancers relied on one dependencies are attractive targets for new cancer of the two VPS4 genes for their survival, including therapies. pediatric rhabdomyosarcoma and bile duct, bladder, breast, colorectal, esophageal, gastric, In a study published in Cell Reports, scientists from liver, lung, ovarian, and pancreatic cancers. In total, the Cancer Dependency Map (DepMap) project at cell lines representing approximately a third of all the Broad Institute of MIT and Harvard and the cancers harbored a VPS4A or B dependency.
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Article in Press
ARTICLE IN PRESS Gene xx (2003) 1–13 www.elsevier.com/locate/gene Comparative sequence and expression analyses of four mammalian VPS4 genes Andreas Beyera, Sibylle Scheuringa, Sibylle Mu¨llera, Antoaneta Minchevab, Peter Lichterb, Karl Ko¨hrera,* aBiologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universita¨tDu¨sseldorf, Moorenstraße 5, D-40225 Duësseldorf, Germany bDeutsches Krebsforschungszentrum, Abt. Organisation komplexer Genome, DKFZ, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany Received 19 August 2002; received in revised form 21 November 2002; accepted 4 December 2002 Received by B. Dujon Abstract The VPS4 gene is a member of the AAA-family; it codes for an ATPase which is involved in lysosomal/endosomal membrane trafficking. VPS4 genes are present in virtually all eukaryotes. Exhaustive data mining of all available genomic databases from completely or partially sequenced organisms revealed the existence of up to three paralogues, VPS4a, -b, and -c. Whereas in the genome of lower eukaryotes like yeast only one VPS4 representative is present, we found that mammals harbour two paralogues, VPS4a and VPS4b. Most interestingly, the Fugu fish contains a third VPS4 paralogue (VPS4c ). Sequence comparison of the three VPS4 paralogues indicates that the Fugu VPS4c displays sequence features intermediate between VPS4a and VPS4b. Using complete mammalian VPS4a and VPS4b cDNA clones as probes, genomic clones of both VPS4 paralogues in human and mouse were identified and sequenced. The chromosomal loci of all four VPS4 genes were determined by independent methods. A BLAST search of the human genome database with the human VPS4A sequence yielded a double match, most likely due to a faulty assembly of sequence contigs in the human draft sequence.
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Vaccinia Virus Hijacks ESCRT-Mediated Multivesicular Body Formation for Virus Egress
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