Anti-VPS4A antibody, Mouse monoclonal clone VPS4-110, purified from hybridoma cell culture Product Number SAB4200215 Product Description Reagent Monoclonal Anti-VPS4A (mouse IgG1 isotype) is Supplied as a solution in 0.01 M phosphate buffered derived from the hybridoma VPS4-110 produced by saline, pH 7.4, containing 15 mM sodium azide as a the fusion of mouse myeloma cells and splenocytes preservative. from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human VPS4A Antibody concentration: 1.0 mg/mL (GeneID: 27183), conjugated to KLH. The corresponding sequence is identical in mouse and rat Precautions and Disclaimer and differs by 3 amino acids in human VPS4B.The For R&D use only. Not for drug, household, or other isotype is determined by ELISA using Mouse uses. Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The antibody is purified from culture Storage/Stability supernatant of hybridoma cells grown in a bioreactor. For continuous use, store at 2–8 C for up to one month. For extended storage, freeze at –20 oC in Monoclonal Anti-VPS4A recognizes human, mouse, working aliquots. Repeated freezing and thawing, or rat, bovine, and canine VPS4A. The antibody may be storage in “frost-free” freezers, is not recommended. If used in several immunochemical techniques including slight turbidity occurs upon prolonged storage, clarify immunoblotting (50 kDa) and immunoprecipitation. the solution by centrifugation before use. Working Detection of the VPS4A band by immunoblotting is dilution samples should be discarded if not used within specifically inhibited by the immunizing peptide. 12 hours. Vps4A is a member of the AAA protein family Product Profile (ATPases associated with diverse cellular activities), Immunoblotting: a working antibody concentration of and is the homolog of the yeast Vps4 protein. In 1-2 g/mL is recommended using whole extracts of humans, two paralogs of the yeast protein have been mouse 3T3 or human HEK-293 cells. identified, Vps4A and Vps4B, that share a high degree of amino acid sequence similarity with each other, and Note: In order to obtain best results in various also with yeast Vps4 and mouse Skd1 proteins. The techniques and preparations, it is recommended to mouse Skd1 (suppressor of K+ transport defect 1) has determine optimal working dilutions by titration. been shown to be actually a yeast Vps4 ortholog. References Functional studies indicate that both human paralogs 1. Yoshimori, T., et al., Mol. Biol. Cell, 11, 747-763 associate with the endosomal compartments, and are (2000). involved in intracellular protein trafficking, similar to 2. Stuchell-Brereton, M.D., et al., Nature, 449, 740- Vps4 protein in yeast. Vps4 mediates endosomal 744 (2007). membrane protein sorting, retroviral budding, and 3. Lata, S., et al., Science, 321, 1354-1357 (2008). cytokinesis by recognizing membrane-associated 4. Obita, T., et al., Nature, 449, 735-739 (2007). ESCRT-III assemblies and catalyzing their 5. Wollert, T., et al., Nature, 458, 172-177 (2009). disassembly. The gene encoding Vps4A has been mapped to chromosome 16 while the gene for Vps4B RC,VS,ST,KAA,PHC,MAM 12/20-1 1-5 resides on chromosome 18. ©2020 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip. .