DOCSLIB.ORG

University of Florida Thesis Or Dissertation Formatting

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
University of Florida Thesis Or Dissertation Formatting SELECTED PATHOGENS OF ORNAMENTAL FISHES By PREEYANAN SRIWANAYOS A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE UNIVERSITY OF FLORIDA 2012 1 © 2012 Preeyanan Sriwanayos 2 To my parents for all their love and support throughout my life 3 ACKNOWLEDGMENTS I thank my advisor, Dr. Ruth Francis-Floyd, for her guidance and support during my 3 years of study. I also thank my co-advisor, Dr. Denise Petty, for her tutelage and help throughout my study. Additionally, I gratefully acknowledge the enthusiastic supervision of the other members of my supervisory committee throughout this work: Drs. Thomas Waltzek, Andrew Kane, and Jeffrey Hill. I am grateful to the Royal Thai Government for financial support throughout my study. I thank Segrest Farms, Gibsonton, Florida and Sun Pet Ltd., Atlanta, Georgia for their generous contributions. I thank Drs. Jim Wellehan, Galaxia Cortes-Hinojosa, and Ms. Linda Archer of the Aquatic Pathobiology Laboratory, University of Florida for their assistance and use of their laboratories. I am appreciative to Dr. Mark Stidworthy of International Zoo Veterinary Group, UK for his pathological expertise. I am in debt to Ms. Heather Maness, Dr. Natalie Steckler, and Dr. Claire Erlacher-Reid for their help with data collection. I thank Dr. Charles Cichra of the University of Florida, Department of Fisheries and Aquatic Sciences and Mr. James Colee of the University of Florida, Institute of Food and Agricultural Sciences (IFAS) Statistics Department for their statistical guidance. I also thank Ms. Karen Kelly for her mentoring and guidance in the area of electron microscopy. I am appreciative of my best friends, Atthapol Charoenkietkrai, Pasicha Chaikaew, Suwussa Bamrungsab, and Akeapot Srifa, for their encouragement and friendship provided throughout my study aboard time and especially during this project. And finally to my parents, thank you for your support and love over the many years. Both of them helped me became the woman I am today. 4 TABLE OF CONTENTS page ACKNOWLEDGMENTS .................................................................................................. 4 LIST OF TABLES ............................................................................................................ 7 LIST OF FIGURES .......................................................................................................... 8 ABSTRACT ................................................................................................................... 10 CHAPTER 1 INTRODUCTION .................................................................................................... 12 Ornamental Fish Trade ........................................................................................... 12 Diseases of Ornamental Fishes .............................................................................. 13 Tetrahymena Infection in Imported Guppies ........................................................... 16 Guppies ............................................................................................................ 16 Tetrahymena .................................................................................................... 19 Megalocytivirus Infection in Fishes, with Emphasis on the Orbiculate Batfish (Platax orbicularis) ............................................................................................... 23 Orbiculate Batfish ............................................................................................. 23 Megalocytivirus ................................................................................................. 24 2 PRELIMINARY ASSESSMENT OF PARASITE LOAD IN SELECTED IMPORTED GUPPIES (POECILIA RETICULATA) ................................................. 37 Materials and Methods............................................................................................ 39 Imported Guppy Population .............................................................................. 39 Gross Examination ........................................................................................... 40 Collection of Tissues for Evaluation of Microscopically Visible Parasites ......... 40 Parasite Identification Protocols ....................................................................... 41 Data Analyses .................................................................................................. 41 Results .................................................................................................................... 42 Discussion .............................................................................................................. 43 3 PRELIMINARY STUDY OF TRANSMISSION OF TETRAHYMENA FROM INFECTED TO TETRAHYMENA-FREE GUPPIES (POECILIA RETICULATA) ..... 51 Materials and Methods............................................................................................ 52 Experimental Animals ....................................................................................... 52 Tetrahymena-free fish ................................................................................ 52 Tetrahymena-infected fish ......................................................................... 54 Experimental Infection ...................................................................................... 55 Histological Examination .................................................................................. 57 Statistical Analysis ............................................................................................ 57 5 Results .................................................................................................................... 57 Experimental Infection ...................................................................................... 57 Histological Examination .................................................................................. 59 Discussion .............................................................................................................. 59 4 MEGALOCYTIVIRUS INFECTION IN ORBICULATE BATFISH (PLATAX ORBICULARIS) ...................................................................................................... 72 Materials and Methods............................................................................................ 75 Clinical History .................................................................................................. 75 Histopathology .................................................................................................. 76 Transmission Electron Microscopy ................................................................... 76 DNA Extraction, Polymerase Chain Reaction Amplification, and Sequencing.. 77 BLASTN, Molecular Dataset, Sequence Alignment, and Phylogenetic Analysis ......................................................................................................... 77 Results .................................................................................................................... 78 Histopathology .................................................................................................. 78 Transmission Electron Microscopy ................................................................... 79 Sequencing, BLASTP, Molecular Dataset, and Phylogenetic Analysis ............ 79 Discussion .............................................................................................................. 80 LIST OF REFERENCES ............................................................................................... 89 BIOGRAPHICAL SKETCH .......................................................................................... 101 6 LIST OF TABLES Table page 1-1 Reports of viral diseases in ornamental fishes ................................................... 28 1-2 Comparison of known megalocytiviruses infecting fishes. .................................. 29 2-1 Microscopically visible parasite were found on external surfaces and within the gastrointestinal tract of guppies imported to the U.S. from Singapore .......... 47 2-2 Determining the number of fish that need to be sampled in order to assess pathogen prevalence in a lot (or population) of fish ............................................ 48 3-1 Classification of parasite intensity per field of view (FOV) at predetermined magnifications ..................................................................................................... 63 3-2 Mortality rates, infection rates, and biopsy findings of 42 exposed Tetrahymena-free female guppies from each treatment during the 14-day experimental period. ........................................................................................... 64 3-3 Mortality rates, infection rates, and biopsy findings of infected male guppies during the 14-day experimental period. .............................................................. 65 4-1 Primers used to amplify fragments of the major capsid protein (MCP) gene sequence from the orbiculate batfish iridovirus (OBIV) ....................................... 82 7 LIST OF FIGURES Figure page 1-1 An example of a fancy guppy (Poecilia reticulata) .............................................. 30 1-2 A photomicrograph of Tetrahymena sp. on the skin mucus of a guppy .............. 31 1-3 A diagram shows the standard shapes of guppy caudal fin ................................ 32 1-4 Differentiation of male and
Load more

Recommended publications
  • §4-71-6.5 LIST of CONDITIONALLY APPROVED ANIMALS November
    §4-71-6.5 LIST OF CONDITIONALLY APPROVED ANIMALS November 28, 2006 SCIENTIFIC NAME COMMON NAME INVERTEBRATES PHYLUM Annelida CLASS Oligochaeta ORDER Plesiopora FAMILY Tubificidae Tubifex (all species in genus) worm, tubifex PHYLUM Arthropoda CLASS Crustacea ORDER Anostraca FAMILY Artemiidae Artemia (all species in genus) shrimp, brine ORDER Cladocera FAMILY Daphnidae Daphnia (all species in genus) flea, water ORDER Decapoda FAMILY Atelecyclidae Erimacrus isenbeckii crab, horsehair FAMILY Cancridae Cancer antennarius crab, California rock Cancer anthonyi crab, yellowstone Cancer borealis crab, Jonah Cancer magister crab, dungeness Cancer productus crab, rock (red) FAMILY Geryonidae Geryon affinis crab, golden FAMILY Lithodidae Paralithodes camtschatica crab, Alaskan king FAMILY Majidae Chionocetes bairdi crab, snow Chionocetes opilio crab, snow 1 CONDITIONAL ANIMAL LIST §4-71-6.5 SCIENTIFIC NAME COMMON NAME Chionocetes tanneri crab, snow FAMILY Nephropidae Homarus (all species in genus) lobster, true FAMILY Palaemonidae Macrobrachium lar shrimp, freshwater Macrobrachium rosenbergi prawn, giant long-legged FAMILY Palinuridae Jasus (all species in genus) crayfish, saltwater; lobster Panulirus argus lobster, Atlantic spiny Panulirus longipes femoristriga crayfish, saltwater Panulirus pencillatus lobster, spiny FAMILY Portunidae Callinectes sapidus crab, blue Scylla serrata crab, Samoan; serrate, swimming FAMILY Raninidae Ranina ranina crab, spanner; red frog, Hawaiian CLASS Insecta ORDER Coleoptera FAMILY Tenebrionidae Tenebrio molitor mealworm,
    [Show full text]
  • Petition to List Eight Species of Pomacentrid Reef Fish, Including the Orange Clownfish and Seven Damselfish, As Threatened Or Endangered Under the U.S
    BEFORE THE SECRETARY OF COMMERCE PETITION TO LIST EIGHT SPECIES OF POMACENTRID REEF FISH, INCLUDING THE ORANGE CLOWNFISH AND SEVEN DAMSELFISH, AS THREATENED OR ENDANGERED UNDER THE U.S. ENDANGERED SPECIES ACT Orange Clownfish (Amphiprion percula) photo by flickr user Jan Messersmith CENTER FOR BIOLOGICAL DIVERSITY SUBMITTED SEPTEMBER 13, 2012 Notice of Petition Rebecca M. Blank Acting Secretary of Commerce U.S. Department of Commerce 1401 Constitution Ave, NW Washington, D.C. 20230 Email: [email protected] Samuel Rauch Acting Assistant Administrator for Fisheries NOAA Fisheries National Oceanographic and Atmospheric Administration 1315 East-West Highway Silver Springs, MD 20910 E-mail: [email protected] PETITIONER Center for Biological Diversity 351 California Street, Suite 600 San Francisco, CA 94104 Tel: (415) 436-9682 _____________________ Date: September 13, 2012 Shaye Wolf, Ph.D. Miyoko Sakashita Center for Biological Diversity Pursuant to Section 4(b) of the Endangered Species Act (“ESA”), 16 U.S.C. § 1533(b), Section 553(3) of the Administrative Procedures Act, 5 U.S.C. § 553(e), and 50 C.F.R.§ 424.14(a), the Center for Biological Diversity hereby petitions the Secretary of Commerce and the National Oceanographic and Atmospheric Administration (“NOAA”), through the National Marine Fisheries Service (“NMFS” or “NOAA Fisheries”), to list eight pomacentrid reef fish and to designate critical habitat to ensure their survival. The Center for Biological Diversity (“Center”) is a non-profit, public interest environmental organization dedicated to the protection of imperiled species and their habitats through science, policy, and environmental law. The Center has more than 350,000 members and online activists throughout the United States.
    [Show full text]
  • RAJA AMPAT MARINE PARK, INDONESIA: the Heart of the Coral Triangle — Conservation Atlas 12/26/18, 10:08 AM
    RAJA AMPAT MARINE PARK, INDONESIA: The Heart of the Coral Triangle — Conservation Atlas 12/26/18, 10:08 AM DECEMBER 12, 2018 RAJA AMPAT MARINE PARK, INDONESIA: The Heart of the Coral Triangle Text: Andreea Lotak; Photographs: Justin & Andreea Lotak • 10 min read The stunning beauty of the Fam Islands as seen from above, Raja Ampat There aren’t many places left on Earth with ecosystems as healthy as those in the Raja Ampat Marine Park. There are over 1,700 species of fish here — with a few dozens of them found nowhere else — swimming among what represents 76% of the world’s coral diversity. The park’s crystal waters protect an area stretching across 35,000 sq km (13,500 sq mi), where large gatherings of manta rays, sharks, whales, mollusks, fish and other aquatic organisms are attracted by the rich nutrients brought by strong oceanic currents. Among all this natural beauty there are roughly 50,000 residents living in villages, settlements and the small town of Waisai, surrounded by limestone cliffs shooting from the turquoise waters and by dense forests where the loud birdsong creates a perfect musical background for a tropical paradise. https://www.conservationatlas.org/blog/raja-ampat-marine-park-indonesia-the-heart-of-the-coral-triangle2018 Page 1 of 15 RAJA AMPAT MARINE PARK, INDONESIA: The Heart of the Coral Triangle — Conservation Atlas 12/26/18, 10:08 AM The Coral Triangle is located at the confluence of the Western Pacific and Indian Oceans, spread across the waters and coastlines of six countries: Indonesia, the Philippines, Papua New Guinea, Malaysia, Solomon Islands and Timor Leste.
    [Show full text]
  • Megalocytivirus Induces Complicated Fish Immune Response at Multiple RNA Levels Involving Mrna, Mirna, and Circrna
    International Journal of Molecular Sciences Article Megalocytivirus Induces Complicated Fish Immune Response at Multiple RNA Levels Involving mRNA, miRNA, and circRNA Qian Wu 1,2,3, Xianhui Ning 1 and Li Sun 1,2,3,* 1 CAS and Shandong Province Key Laboratory of Experimental Marine Biology, CAS Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China; [email protected] (Q.W.); [email protected] (X.N.) 2 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, China 3 College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China * Correspondence: [email protected]; Tel.: +86-532-82898829 Abstract: Megalocytivirus is an important viral pathogen to many farmed fishes, including Japanese flounder (Paralichthys olivaceus). In this study, we examined megalocytivirus-induced RNA responses in the spleen of flounder by high-throughput sequencing and integrative analysis of various RNA- seq data. A total of 1327 microRNAs (miRNAs), including 368 novel miRNAs, were identified, among which, 171 (named DEmiRs) exhibited significantly differential expressions during viral infection in a time-dependent manner. For these DEmiRs, 805 differentially expressed target mRNAs (DETmRs) were predicted, whose expressions not only significantly changed after megalocytivirus infection but were also negatively correlated with their paired DEmiRs. Integrative analysis of Citation: Wu, Q.; Ning, X.; Sun, L. Megalocytivirus Induces immune-related DETmRs and their target DEmiRs identified 12 hub DEmiRs, which, together with Complicated Fish Immune Response their corresponding DETmRs, formed an interaction network containing 84 pairs of DEmiR and at Multiple RNA Levels Involving DETmR.
    [Show full text]
  • Luth Wfu 0248D 10922.Pdf
    SCALE-DEPENDENT VARIATION IN MOLECULAR AND ECOLOGICAL PATTERNS OF INFECTION FOR ENDOHELMINTHS FROM CENTRARCHID FISHES BY KYLE E. LUTH A Dissertation Submitted to the Graduate Faculty of WAKE FOREST UNIVERSITY GRADAUTE SCHOOL OF ARTS AND SCIENCES in Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY Biology May 2016 Winston-Salem, North Carolina Approved By: Gerald W. Esch, Ph.D., Advisor Michael V. K. Sukhdeo, Ph.D., Chair T. Michael Anderson, Ph.D. Herman E. Eure, Ph.D. Erik C. Johnson, Ph.D. Clifford W. Zeyl, Ph.D. ACKNOWLEDGEMENTS First and foremost, I would like to thank my PI, Dr. Gerald Esch, for all of the insight, all of the discussions, all of the critiques (not criticisms) of my works, and for the rides to campus when the North Carolina weather decided to drop rain on my stubborn head. The numerous lively debates, exchanges of ideas, voicing of opinions (whether solicited or not), and unerring support, even in the face of my somewhat atypical balance of service work and dissertation work, will not soon be forgotten. I would also like to acknowledge and thank the former Master, and now Doctor, Michael Zimmermann; friend, lab mate, and collecting trip shotgun rider extraordinaire. Although his need of SPF 100 sunscreen often put our collecting trips over budget, I could not have asked for a more enjoyable, easy-going, and hard-working person to spend nearly 2 months and 25,000 miles of fishing filled days and raccoon, gnat, and entrail-filled nights. You are a welcome camping guest any time, especially if you do as good of a job attracting scorpions and ants to yourself (and away from me) as you did on our trips.
    [Show full text]
  • Understanding Transformative Forces of Aquaculture in the Marine Aquarium Trade
    The University of Maine DigitalCommons@UMaine Electronic Theses and Dissertations Fogler Library Summer 8-22-2020 Senders, Receivers, and Spillover Dynamics: Understanding Transformative Forces of Aquaculture in the Marine Aquarium Trade Bryce Risley University of Maine, [email protected] Follow this and additional works at: https://digitalcommons.library.umaine.edu/etd Part of the Marine Biology Commons Recommended Citation Risley, Bryce, "Senders, Receivers, and Spillover Dynamics: Understanding Transformative Forces of Aquaculture in the Marine Aquarium Trade" (2020). Electronic Theses and Dissertations. 3314. https://digitalcommons.library.umaine.edu/etd/3314 This Open-Access Thesis is brought to you for free and open access by DigitalCommons@UMaine. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of DigitalCommons@UMaine. For more information, please contact [email protected]. SENDERS, RECEIVERS, AND SPILLOVER DYNAMICS: UNDERSTANDING TRANSFORMATIVE FORCES OF AQUACULTURE IN THE MARINE AQUARIUM TRADE By Bryce Risley B.S. University of New Mexico, 2014 A THESIS Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science (in Marine Policy and Marine Biology) The Graduate School The University of Maine May 2020 Advisory Committee: Joshua Stoll, Assistant Professor of Marine Policy, Co-advisor Nishad Jayasundara, Assistant Professor of Marine Biology, Co-advisor Aaron Strong, Assistant Professor of Environmental Studies (Hamilton College) Christine Beitl, Associate Professor of Anthropology Douglas Rasher, Senior Research Scientist of Marine Ecology (Bigelow Laboratory) Heather Hamlin, Associate Professor of Marine Biology No photograph in this thesis may be used in another work without written permission from the photographer.
    [Show full text]
  • An Invasive Fish and the Time-Lagged Spread of Its Parasite Across the Hawaiian Archipelago
    An Invasive Fish and the Time-Lagged Spread of Its Parasite across the Hawaiian Archipelago Michelle R. Gaither1,2*, Greta Aeby2, Matthias Vignon3,4, Yu-ichiro Meguro5, Mark Rigby6,7, Christina Runyon2, Robert J. Toonen2, Chelsea L. Wood8,9, Brian W. Bowen2 1 Ichthyology, California Academy of Sciences, San Francisco, California, United States of America, 2 Hawai’i Institute of Marine Biology, University of Hawai’i at Ma¯noa, Kane’ohe, Hawai’i, United States of America, 3 UMR 1224 Ecobiop, UFR Sciences et Techniques Coˆte Basque, Univ Pau and Pays Adour, Anglet, France, 4 UMR 1224 Ecobiop, Aquapoˆle, INRA, St Pe´e sur Nivelle, France, 5 Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Japan, 6 Parsons, South Jordan, Utah, United States of America, 7 Marine Science Institute, University of California Santa Barbara, Santa Barbara, California, United States of America, 8 Department of Biology, Stanford University, Stanford, California, United States of America, 9 Hopkins Marine Station, Stanford University, Pacific Grove, California, United States of America Abstract Efforts to limit the impact of invasive species are frustrated by the cryptogenic status of a large proportion of those species. Half a century ago, the state of Hawai’i introduced the Bluestripe Snapper, Lutjanus kasmira, to O’ahu for fisheries enhancement. Today, this species shares an intestinal nematode parasite, Spirocamallanus istiblenni, with native Hawaiian fishes, raising the possibility that the introduced fish carried a parasite that has since spread to naı¨ve local hosts. Here, we employ a multidisciplinary approach, combining molecular, historical, and ecological data to confirm the alien status of S.
    [Show full text]
  • Download Download
    Journal ofThreatened JoTT TaxaBuilding evidence for conservation globally 10.11609/jott.2020.12.10.16195-16406 www.threatenedtaxa.org 26 July 2020 (Online & Print) Vol. 12 | No. 10 | Pages: 16195–16406 ISSN 0974-7907 (Online) | ISSN 0974-7893 (Print) PLATINUM OPEN ACCESS Dedicated to Dr. P. Lakshminarasimhan ISSN 0974-7907 (Online); ISSN 0974-7893 (Print) Publisher Host Wildlife Information Liaison Development Society Zoo Outreach Organization www.wild.zooreach.org www.zooreach.org No. 12, Thiruvannamalai Nagar, Saravanampatti - Kalapatti Road, Saravanampatti, Coimbatore, Tamil Nadu 641035, India Ph: +91 9385339863 | www.threatenedtaxa.org Email: [email protected] EDITORS English Editors Mrs. Mira Bhojwani, Pune, India Founder & Chief Editor Dr. Fred Pluthero, Toronto, Canada Dr. Sanjay Molur Mr. P. Ilangovan, Chennai, India Wildlife Information Liaison Development (WILD) Society & Zoo Outreach Organization (ZOO), 12 Thiruvannamalai Nagar, Saravanampatti, Coimbatore, Tamil Nadu 641035, Web Development India Mrs. Latha G. Ravikumar, ZOO/WILD, Coimbatore, India Deputy Chief Editor Typesetting Dr. Neelesh Dahanukar Indian Institute of Science Education and Research (IISER), Pune, Maharashtra, India Mr. Arul Jagadish, ZOO, Coimbatore, India Mrs. Radhika, ZOO, Coimbatore, India Managing Editor Mrs. Geetha, ZOO, Coimbatore India Mr. B. Ravichandran, WILD/ZOO, Coimbatore, India Mr. Ravindran, ZOO, Coimbatore India Associate Editors Fundraising/Communications Dr. B.A. Daniel, ZOO/WILD, Coimbatore, Tamil Nadu 641035, India Mrs. Payal B. Molur, Coimbatore, India Dr. Mandar Paingankar, Department of Zoology, Government Science College Gadchiroli, Chamorshi Road, Gadchiroli, Maharashtra 442605, India Dr. Ulrike Streicher, Wildlife Veterinarian, Eugene, Oregon, USA Editors/Reviewers Ms. Priyanka Iyer, ZOO/WILD, Coimbatore, Tamil Nadu 641035, India Subject Editors 2016–2018 Fungi Editorial Board Ms.
    [Show full text]
  • Conservation and Management of Ornamental Coral Reef Wildlife: Successes, Shortcomings, and Future Directions Q ⇑ Laura E
    Biological Conservation 169 (2014) 225–237 Contents lists available at ScienceDirect Biological Conservation journal homepage: www.elsevier.com/locate/biocon Review Conservation and management of ornamental coral reef wildlife: Successes, shortcomings, and future directions q ⇑ Laura E. Dee a, Stephanie S. Horii a, Daniel J. Thornhill b, a Bren School of Environmental Science & Management, University of California, Santa Barbara, 2400 Bren Hall, Santa Barbara, CA 93106, USA b Department of Conservation Science and Policy, Defenders of Wildlife, 1130 17th Street NW, Washington, DC 20036, USA article info abstract Article history: Trade in ornamental coral reef wildlife supports a multi-million dollar industry but in some places threat- Received 23 August 2013 ens vulnerable coral reef species and ecosystems due to unsustainable practices and lack of effective reg- Received in revised form 12 November 2013 ulation. To supply this trade, fishers sometimes deplete fish populations and rely on practices, such as Accepted 14 November 2013 cyanide fishing, that harm coral reef organisms and habitats. The number of countries involved, dispersed fishing localities, and the diversity of species in trade present considerable impediments to conservation and management. For instance, traditional fisheries management techniques such as stock assessments Keywords: and total catch limits may not be feasible for ornamental fisheries, which are characterized by limited Aquarium trade data on population dynamics, stock status, and collection effort, as well as instances of illegal, underre- Coral reef conservation Coral reef fisheries ported, and unregulated fishing. A number of strategies to monitor, regulate, and manage the trade have Ornamental fisheries been implemented with varying efficacy. In order to learn from previous attempts and identify promising Sustainable fisheries management approaches, we reviewed selected management practices and regulations from diverse settings, with attention to the effectiveness of each approach.
    [Show full text]
  • A New Cryptic Species Of
    Zoosyst. Evol. 95 (2) 2019, 345–360 | DOI 10.3897/zse.95.34069 A new cryptic species of Hyphessobrycon Durbin, 1908 (Characiformes, Characidae) from the Eastern Amazon, revealed by integrative taxonomy Erick Cristofore Guimarães1,3,4, Pâmella Silva de Brito1,3,4, Leonardo Manir Feitosa2, Luis Fernando Carvalho Costa3, Felipe Polivanov Ottoni1,4,5,6 1 Universidade Federal do Maranhão, Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal. Av. dos Portugueses 1966, Cidade Universitária do Bacanga, CEP 65080-805, São Luís, MA, Brazil 2 Universidade Federal de Pernambuco, Programa de Pós-Graduação em Biologia Animal. Av. Professor Moraes Rego 1235, Cidade Universitária, CEP 50670-901, Recife, PE, Brazil 3 Universidade Federal do Maranhão, Departamento de Biologia, Laboratório de Genética e Biologia Molecular, Av. dos Portugueses 1966, Cidade Universitária do Bacanga, CEP 65080-805, São Luís, MA, Brazil 4 Universidade Federal do Maranhão, Laboratório de Sistemática e Ecologia de Organismos Aquáticos, Centro de Ciências Agrárias e Ambientais, Campus Universitário, CCAA, BR-222, KM 04, S/N, Boa Vista, CEP 65500-000, Chapadinha, MA, Brazil 5 Universidade Federal do Maranhão, Programa de Pós-Graduação em Biodiversidade e Conservação. Av. dos Portugueses 1966, Cidade Universitária do Bacanga, CEP 65080-805, São Luís, MA, Brazil 6 Universidade Federal do Maranhão, Programa de Pós-graduação em Ciências Ambientais, Centro de Ciências Agrárias e Ambientais, Campus Universitário, CCAA, BR-222, KM 04, S/N, Boa Vista, CEP 65500-000, Chapadinha, MA, Brazil http://zoobank.org/E45AD907-EFD2-46B9-B056-31250BC8BFEE Corresponding author: Erick Cristofore Guimarães ([email protected]) Academic editor: Nicolas Hubert ♦ Received 25 February 2019 ♦ Accepted 7 May 2019 ♦ Published 12 June 2019 Abstract Hyphessobrycon caru sp.
    [Show full text]
  • IIIIIHIIIIIIINII 3 0692 1078 60« 1' University of Ghana
    University of Ghana http://ugspace.ug.edu.gh SITY Of OMAN* IIBRADV QL391.N4 B51 blthrC.1 G365673 The Balme Library IIIIIHIIIIIIINII 3 0692 1078 60« 1' University of Ghana http://ugspace.ug.edu.gh GUINEA WORM: SOCIO-CULTURAL STUDIES, MORPHOMETRY, HISTOMORPHOLOGY, VECTOR SPECIES AND DNA PROBE FOR DRACUNCULUS SPECIES. A thesis Presented to the Board of Graduate Studies, University of Ghana, Legon. Ghana. In fulfillment of the Requirements for the Degree of Doctor of Philosophy (Ph.D.) (Zoology). Langbong Bimi M. Phil. Department of Zoology, Faculty of Science, University of Ghana Legon, Accra, Ghana. September 2001 University of Ghana http://ugspace.ug.edu.gh DECLARATION I do hereby declare that except for references to other people’s investigations which I duly acknowledged, this exercise is the result of my own original research, and that this thesis, either in whole, or in part, has not been presented for another degree elsewhere. PRINCIPAL INVESTIGATOR SIGNATURE DATE LANGBONG BIMI -==4=^". (STUDENT) University of Ghana http://ugspace.ug.edu.gh 1 DEDICATION This thesis is dedicated to the Bimi family in memory of our late father - Mr. Combian Bimi Dapaah (CBD), affectionately known as Nanaanbuang by his admirers. You never limited us with any preconceived notions of what we could or could not achieve. You made us to understand that the best kind of knowledge to have is that which is learned for its own sake, and that even the largest task can be accomplished if it is done one step at a time. University of Ghana http://ugspace.ug.edu.gh SUPERVISORS NATU DATE 1.
    [Show full text]
  • Hlístice Vybraných Druhů Studenokrevných Obratlovců Západní Afriky Diplomová Práce
    MASARYKOVA UNIVERZITA Přírodovědecká fakulta Ústav botaniky a zoologie Hlístice vybraných druhů studenokrevných obratlovců západní Afriky Diplomová práce Brno 2008 autor: Bc. Šárka Mašová Vedoucí DP: RNDr. Božena Koubková, Ph.D. PROHLÁŠENÍ Souhlasím s uložením této diplomové práce v knihovně Ústavu botaniky a zoologie PřF MU v Brně, případně v jiné knihovně MU, s jejím veřejným půjčováním a využitím pro vědecké, vzdělávací nebo jiné veřejně prospěšné účely, a to za předpokladu, že převzaté informace budou řádně citovány a nebudou využívány komerčně. Brno, 19. května 2008 …………………………….. PODĚKOVÁNÍ Ráda bych poděkovala vedoucí mé diplomové práce RNDr. Boženě Koubkové, Ph.D. za její odborné vedení a praktické rady. Velice děkuji svému konzultantovi prof. Ing. Vlastimilu Barušovi, DrSc. za cenné rady a pomoc při zpracování problematiky taxonomie nematod, prof. RNDr. Františkovi Tenorovi, DrSc. za pomoc s determinací tasemnic, dále Mgr. Ivetě Matějusové, Ph.D. za molekulární analýzy, Mgr. Ivetě Hodové za zasvěcení do SEM a Mgr. Radimovi Sonnekovi za zasvěcení do CLSM a v neposlední řadě Doc. RNDr. Petru Koubkovi, CSc. za poskytnutí studijního materiálu. Také děkuji všem, kteří mi jakýmkoliv způsobem pomohli při zpracování této diplomové práce a svým nejbližším za podporu. Práce byla finančně podporována Grantovou agenturou AV ČR, grant číslo IAA6093404 a výzkumným záměrem Masarykovy university v Brně číslo MSM 0021622416. ABSTRAKT Za účelem studia parazitických hlístic ryb Senegalu bylo v letech 2004 – 2006 vyšetřeno 330 jedinců náležejících ke 49 sladkovodním druhům ryb. Většina vyšetřených ryb pocházela z národního parku Nikolo Koba ve východním Senegalu. Celkem byly determinovány 3 rody parazitických hlístic ve 24 druzích ryb (prevalence 71%) z 9 čeledí. Nalezená fauna hlístic se skládala většinou ze zástupců čeledi Camallanidae.
    [Show full text]